Last data update: 24 January 2024 16:39 CET
Plasmid name: pViridis (LMBP 9846)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p9846.gb
(View with Genome Compiler) p9846.txt p9846.pdf |
| Sequence analysis results Genecorner: |
Sanger: 136495.fasta Sanger: 136496.fasta NGS: f04-gc-dec2018.fasta |
| Cloned DNA: |
- |
| Promoter: | Cauliflower mosaic virus (CaMV) 35S promoter Phage T7 gene 10 promoter (T7g10) Phage T3 promoter Escherichia coli lac operon promoter |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | Cauliflower mosaic virus 35S terminator (CaMV 35S) |
| Selection marker: | Hygromycin (hyg) Neomycin (neo; kanamycin (kan)) |
| Replicon: | Escherichia coli plasmid pMB1 origin Broad-host-range plasmid pSa replication origin |
| Host range: | Escherichia coli Plant cells |
| Parental clone: | pGreenII |
| Further information: | The plasmid was constructed by deleting a 1.3 kb Bal31 fragment from pGreenII (version 0179), removing much of the IS5 element and extended into the ColE1 origin of replication. The plasmid is derived from pGreenII which is widely used to produce plant transformants via a process that involves propagation in E. coli. pGreenII-based constructs can be unstable in E. coli as a consequence of them hampering cell division and promoting cell death. pViridis does not cause these effects, thereby removing the selective pressure for mutation. pViridis should help to ensure the integrity of genes destined for study in plants, while they are propagated and manipulated in E. coli. The nucleotide sequence of the T-DNA region of the pGreenII (version 0179) vector corresponds with the EMBL Nucleotide Sequence Database accession number EU048866.1. |
| EMBL Accession number: | EU048866.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 14/07/2016 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: EcoRI, PvuI and XmnI. This plasmid has also been fully sequenced but the NGS sequence data still need to be implemented. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by Prof. Dr P. Meyer(1). It was constructed by M. Watson (1). (1) Plant Genetics Group, Centre for Plant Sciences, School of Biology, Faculty of Biological Sciences, University of Leeds, Leeds, United Kingdom. |
| Plasmid reference: | Watson et al., G3 (Bethesda) 6 (2016), 2195-2201 [PMID: 27194805] [DOI: 10.1534/g3.116.029405] |
| Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pViridis (LMBP 9846) is available at BCCM/GeneCorner. The plasmid was deposited by Prof. Dr P. Meyer and was published in Watson et al., 2016. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.