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GeneCorner plasmid details

Last data update: 24 May 2019 04:16 CEST  


Name: pViridis   Add to cart
Accession number: LMBP 9846
Sequence file: LMBP sequence: p9846.gb, p9846.txt     View with Genome Compiler
Circular map: p9846.pdf
Status: GeneCorner core plasmid
Cloned DNA: -
Promoter: Cauliflower mosaic virus (CaMV) 35S promoter
Escherichia coli lac operon promoter
Phage T7 gene 10 promoter (T7g10)
Phage T3 promoter
Ribosome
binding site:
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ)
Terminator: Cauliflower mosaic virus 35S terminator (CaMV 35S)
Selection marker: Hygromycin (hyg)
Neomycin (neo; kanamycin (kan))
Replicon: Escherichia coli plasmid pMB1 origin
Escherichia coli plasmid pSa origin
Host range: Escherichia coli
Plant cells
Parental clone: pGreenII
Further information: The plasmid was constructed by deleting a 1.3 kb Bal31 fragment from pGreenII (version 0179), removing much of the IS5 element and extended into the ColE1 origin of replication.
The plasmid is derived from pGreenII which is widely used to produce plant transformants via a process that involves propagation in E. coli.
pGreenII-based constructs can be unstable in E. coli as a consequence of them hampering cell division and promoting cell death.
pViridis does not cause these effects, thereby removing the selective pressure for mutation. pViridis should help to ensure the integrity of genes destined for study in plants, while they are propagated and manipulated in E. coli.
The nucleotide sequence of the T-DNA region of the pGreenII (version 0179) vector corresponds with the EMBL Nucleotide Sequence Database accession number EU048866.1.
EMBL Accession number: EU048866.1, view at EMBL, GenBank, DDBJ
Latest sequence update: 14/07/2016
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: EcoRI, PvuI and XmnI.

This plasmid has also been fully sequenced but the NGS sequence data still needs to be implemented.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: The plasmid was deposited by Prof. Dr P. Meyer(1). It was constructed by M. Watson (1).
(1) Plant Genetics Group, Centre for Plant Sciences, School of Biology, Faculty of Biological Sciences, University of Leeds, Leeds, United Kingdom.
Plasmid reference: Watson et al., G3 (Bethesda) (2016), Epub [PMID: 27194805] [DOI: 10.1534/g3.116.029405]
Restricted distribution: - BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + kanamycin (50 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

 

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