GREAT AT SMALL THINGS

GeneCorner plasmid details

Last data update: 15 July 2019 04:12 CEST  


Name: pdcDNA3-R4R3   Add to cart
Accession number: LMBP 9009
Sequence file: Depositor's sequence: p9009.gb     View with Genome Compiler
Status: GeneCorner non-core plasmid
Cloned DNA: B gene of the control of cell death locus of the Escherichia coli F plasmid (ccdB, lethal gene)
Promoter: Human cytomegalovirus immediate early promoter (CMV-IE) and enhancer
Simian virus 40 early promoter (SV40 early)
Phage T7 gene 10 promoter (T7g10)
Phage SP6 promoter
Escherichia coli lac operon promoter
Escherichia coli lac operon promoter; mutant (lacUV5)
Ribosome
binding site:
-
Terminator: Bovine growth hormone polyadenylation signal (BGH polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
Selection marker: Ampicillin (amp)
Chloramphenicol (cam)
Neomycin (neo; G418)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Simian virus 40 bidirectional origin (SV40)
Host range: Escherichia coli; use a ccdB-resistant strain for propagation
Mammalian cells
Parental clone: pcDNA3; pLenti6 pDEST pLenti + CMV MGW
Further information: This multisite Gateway destination vector was constructed by inserting the PCR-amplified attR4-R3 multisite Gateway cassette into the HindIII/XhoI-opened pcDNA3 plasmid. The Gateway multisite cassette was PCR-amplified from pLenti6 pDEST pLenti + CMV MGW.
EMBL Accession number: -
Latest sequence update: 28/02/2014
Authenticity test: Restriction enzyme pattern analysed at GeneCorner: EcoRI and NcoI.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: This plasmid was deposited by Dr M. De Ceuleneer(1) and Prof. Dr R. Beyaert(1).
(1) BCCM/GeneCorner, Department of Biomedical Molecular Biology, Ghent University, Ghent, Belgium
Plasmid reference: -
Restricted distribution: - BCCM MTA
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 28°C
Biosafety level: L1
Cultivation remark: -
Other culture collection numbers: -

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

 

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