Last data update: 24 January 2024 16:39 CET
Plasmid name: pC2HP-Pho85 (LMBP 10593)
New search | Print data sheet |
Price category: | Cat. 1 (cf. price list) |
Status: | GeneCorner non-core plasmid |
Depositor's sequence: | p10593.gb |
Sequence analysis results Genecorner: |
- |
Cloned DNA: |
Candida albicans cyclin-dependent serine/threonine-protein kinase cDNA (PHO85, GeneID 3646148) Viral protein 16 activation domain (VP16ad, AD VP16); N-terminal SV40 large T-antigen nuclear localization signal (NLS); N-terminal FLAG epitope tag; N-terminal |
Promoter: | Candida albicans sulfate adenylyltransferase promoter (MET3) Phage SP6 promoter Escherichia coli lac operon promoter Escherichia coli ß-lactamase promoter (amp) |
Ribosome binding site: |
- |
Terminator: | Candida albicans actin terminator (ACT1) |
Selection marker: | Neomycin (neo; kanamycin (kan)) Candida dubliniensis ARG4 (CdARG4); auxotrophic |
Replicon: | Escherichia coli plasmid pMB1 origin |
Host range: | Escherichia coli Candida albicans; arg4(-), integrative |
Parental clone: | pC2HP-GC |
Further information: | The plasmid was constructed by cloning the C. albicans PHO85 coding sequence into the pC2HP-GC vector via Gateway LR recombination. This is a prey plasmid for yeast two-hybrid analysis. The plasmid can be linearised by NotI restriction to allow genomic integration. |
EMBL Accession number: | - |
Latest sequence update: | 07/08/2018 |
Authenticity test: | The plasmid still needs to be subjected to the authenticity test. |
Class: | Recombinant plasmid |
Type: | Plasmid |
History of deposit: | This plasmid was deposited by Prof. Dr P. Van Dijck(1)(2). It was constructed by F. Schoeters(1)(2). (1) VIB-KU Leuven Center for Microbiology, VIB, Leuven, Belgium (2) Laboratory of Molecular Cell Biology, Institute of Botany and Microbiology, KU Leuven, Leuven, Belgium |
Plasmid reference: | Schoeters et al., mSphere 3 (2018), e00391-18 [PMID: 30135223] [DOI: 10.1128/mSphere.00391-18] |
Related plasmid reference: | Schoeters et al., Front. Microbiol. 10 (2019), 1792 [PMID: 31440220] [DOI: 10.3389/fmicb.2019.01792] Legrand et al., Nucleic Acids Res. 46 (2018), 6935-6949 [PMID: 29982705] [DOI: 10.1093/nar/gky594] |
Restricted distribution: | - BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
Distributed as: | H/P active culture or plasmid DNA |
Host for distribution: | Escherichia coli K12 MC1061 |
Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
Cultivation medium: | LB-Lennox + kanamycin (50 μg/ml) |
Cultivation temperature: | 37°C |
Biosafety level: | L1 |
Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pC2HP-Pho85 (LMBP 10593) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr P. Van Dijck and was published in Schoeters et al., 2018. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.