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GeneCorner plasmid details

Last data update: 24 January 2024 16:39 CET

Plasmid name: pET-Duet-Hth-CCSα/β (LMBP 11134)

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Price category: Cat. 3 (cf. price list)
Status: GeneCorner non-core plasmid
Depositor's sequence: p11134.gb
Sequence
analysis results
Genecorner:

NGS: g02-gc-dec2018.fasta

Cloned DNA: Hydrogenobacter thermophilus citryl-CoA synthetase cDNA (CCS)
Escherichia coli lac repressor gene (lacI)
Histidine tag (His-tag); C-terminal
Promoter: Phage T7 gene 10 promoter (T7g10) with lac operator (T7lac)
Ribosome
binding site:
Ribosome binding site (RBS) of the phage T7 gene 10 (T7g10)
Terminator: Phage T7 gene 10 terminator (T7g10)
Selection marker: Ampicillin (amp)
Replicon: Escherichia coli plasmid pMB1 origin
Phage f1 origin
Host range: Escherichia coli
Parental clone: pETDuet-1
Further information: The plasmid was constructed by cloning codon optimized cDNA fragments encoding the H. thermophilus CCSα (Uniprot ID Q75VW6) and CCSβ (Uniprot ID D3DK29) subunits coding sequences into the pETDuet-1 vector, with the CCSβ fragment carrying a C-terminal His-tag.
Other names of the plasmid are 357_CCSbeta_Htherm_CCSalpha_Htherm_pETDuet-1 and pETDuet1-HthermCCSbeta-HthermCCSalpha.
EMBL Accession number: -
Latest sequence update: 11/02/2019
Authenticity test: This plasmid has been fully sequenced.
Class: Recombinant plasmid
Type: Plasmid
History of deposit: The plasmid was deposited by A. Dansercoer(1)(2), Dr K. Verstraete(1)(2) and Prof. Dr S. Savvides(1)(2).
1) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium
(2) VIB-UGent Center for Inflammation Research, VIB, Ghent, Belgium
Plasmid reference: Verschueren et al., Nature 568 (2019), 571-575 [PMID: 30944476] [DOI: 10.1038/s41586-019-1095-5]
Restricted distribution: - VIB/BCCM MTA
- The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC.
Distributed as: H/P active culture or plasmid DNA
Host for distribution: Escherichia coli K12 DH5α
Host reference: Focus 8 (1986), 9
Related host reference: Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660]
Rodriguez-Quinones et al., Focus 15 (1993), 110-112
Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8]
Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187]
Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051]
Cultivation medium: LB-Lennox + ampicillin (100 μg/ml)
Cultivation temperature: 37°C
Biosafety level: L1
Other culture collection numbers: -

Refer in your Materials and Methods:

pET-Duet-Hth-CCSα/β (LMBP 11134) is available at BCCM/GeneCorner. The plasmid was deposited by A. Dansercoer, Dr K. Verstraete and Prof. Dr S. Savvides and was published in Verschueren et al., 2019.

Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.

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