Last data update: 24 January 2024 16:39 CET
Plasmid name: pTrc-His2-hACLY (LMBP 11277)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p11277.gb
(View with Genome Compiler) p11277.txt p11277.pdf |
| Sequence analysis results Genecorner: |
NGS: a02-gc-dec2019.fasta |
| Cloned DNA: |
Human ATP citrate lyase cDNA (ACLY, ATPCL, CLATP, ACL, GeneID 47) Escherichia coli lac repressor gene (lacI) Myc epitope; C-terminal Histidine tag (His-tag); C-terminal |
| Promoter: | Escherichia coli hybrid tryptophan/lacUV5 promoter (trc) Escherichia coli lac repressor promoter; mutant (lacI(q)) |
| Ribosome binding site: |
Ribosome binding site (RBS) of the Escherichia coli lac Z gene (lacZ) |
| Terminator: | Escherichia coli rrnB operon T1 terminator Escherichia coli rrnB operon T2 terminator |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin |
| Host range: | Escherichia coli |
| Parental clone: | pTrcHis2-TOPO |
| Further information: | The plasmid was constructed by preparing the human ACLY (Uniprot ID P53396-2) coding sequence from poly A+ RNA from human liver (Clontech) and cloning it into the pTrcHis2-TOPO vector, in frame with the C-terminal Myc epitope and His-tag. The human ACLY coding sequence corresponds with isoform 2, which differs from the canonical sequence in the fact that it is shorter at the N-terminus and is lacking an internal segment. The nucleotide sequence of the human ACLY coding sequence corresponds with the Genbank accession number NM_198830.1. Other names of the plasmid are pTRcHis2-hACL_full_length, pTrc-His2-hACL and pTRcHis2-hACL_WT. |
| EMBL Accession number: | NM_198830.1, view at GenBank |
| Latest sequence update: | 13/05/2020 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI and NcoI. The plasmid has also been fully sequenced. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | The plasmid was deposited by A. Dansercoer(1)(2), Dr K. Verstraete(1)(2) and Prof. Dr S. Savvides(1)(2). (1) Department of Biochemistry and Microbiology, Ghent University, Ghent, Belgium (2) VIB-UGent Center for Inflammation Research, VIB, Ghent, Belgium |
| Plasmid reference: | Verschueren et al., Nature 568 (2019), 571-575 [PMID: 30944476] [DOI: 10.1038/s41586-019-1095-5] |
| Restricted distribution: | - VIB/BCCM MTA - The depositor will be informed of the customer's identity upon release of a sample outside the depositor's department or outside the departments in which BCCM/GeneCorner is embedded, namely UGent-DBMB and VIB-IRC. |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 DH5α |
| Host reference: | Focus 8 (1986), 9 |
| Related host reference: | Woodcock et al., Nucleic Acids Res. 17 (1989), 3469-3478 [PMID: 2657660] Rodriguez-Quinones et al., Focus 15 (1993), 110-112 Hanahan, J. Mol. Biol. 166 (1983), 557-580 [PMID: 6345791] [DOI: 10.1016/s0022-2836(83)80284-8] Hanahan, in 'DNA Cloning: A Practical Approach Volume I', IRL Press, Oxford (1985), 109-135 [ISSN/ISBN: 0947946187] Grant et al., Proc. Natl. Acad. Sci. U.S.A. 87 (1990), 4645-4649 [PMID: 2162051] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pTrc-His2-hACLY (LMBP 11277) is available at BCCM/GeneCorner. The plasmid was deposited by A. Dansercoer, Dr K. Verstraete and Prof. Dr S. Savvides and was published in Verschueren et al., 2019. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.