Last data update: 24 January 2024 16:39 CET
Plasmid name: pCAGGS (LMBP 2453)
| New search | Print data sheet |
| Price category: | Cat. 3 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p2453.gb
(View with Genome Compiler) p2453.txt p2453.pdf |
| Sequence analysis results Genecorner: |
|
| Cloned DNA: |
- |
| Promoter: | Chicken β-actin/rabbit β-globin hybrid promoter (AG) Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only Escherichia coli lac operon promoter |
| Ribosome binding site: |
- |
| Terminator: | Rabbit β-globin polyadenylation signal (β-globin polyA) Simian virus 40 polyadenylation signal (SV40 polyA) |
| Selection marker: | Ampicillin (amp) |
| Replicon: | Escherichia coli plasmid pMB1 origin Simian virus 40 bidirectional origin (SV40) |
| Copy number: | High copy number |
| Host range: | Escherichia coli Mammalian cells; SV40 permissive cells |
| Parental clone: | pUC13; pAGS-3 |
| Further information: | This plasmid is useful for highly efficient expression of genes under the control of the AG promoter and the human CMV-IE enhancer in various mammalian cells. The AG promoter sequence consists of the chicken β-actin promoter, the first exon and part of the first intron (that seems to have a strong enhancer-like activity) linked to a rabbit β-globin fragment, consisting of a 3' part of the second intron (inclusive a branch point which is required for normal splicing reactions) and a 5' part of the third exon. When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727518.1 except for the adjustments due to the implementation of the next-generation sequencing results. |
| EMBL Accession number: | LT727518.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 05/11/2019 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BglII, EcoRI, NcoI, SacII and XhoI. This plasmid has also been fully sequenced. For some nucleotides, different NGS results contradict each other, in which case the sequence info from the depositor was kept. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Prof. Dr J. Miyazaki(1). (1) University of Tokyo, Japan |
| Plasmid reference: | Niwa et al., Gene 108 (1991), 193-200 [PMID: 1660837] |
| Related plasmid reference: | Lee et al., Mol. Cancer 10 (2011), 113 [PMID: 21917150] [DOI: 10.1186/1476-4598-10-113] Thorrez et al., Mol. Ther. 14 (2006), 442-451 [PMID: 16750937] [DOI: 10.1016/j.ymthe.2006.03.019] Verhelst et al., EMBO J. 31 (2012), 3845-3855 [PMID: 23032186] [DOI: 10.1038/emboj.2012.240] Staal et al., bioRxiv (2017), 1-25 [DOI: 10.1101/228767] Pattabriaman et al., J. Cell Biol. 208 (2015), 53-69 [PMID: 25547156] [DOI: 10.1083/jcb.201404109] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 MC1061 |
| Host reference: | Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493] |
| Related host reference: | Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111] |
| Cultivation medium: | LB-Lennox + ampicillin (100 μg/ml) |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | - |
| Other culture collection numbers: | - |
Refer in your Materials and Methods: |
pCAGGS (LMBP 2453) is available at BCCM/GeneCorner. This plasmid was deposited by Prof. Dr J. Miyazaki and was published in Niwa et al., 1991. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.