Last data update: 31 March 2020 04:18 CEST
Plasmid name: pCAGGS (LMBP 2453)
|Price category:||Cat. 2 (cf. price list)|
|Status:||GeneCorner core plasmid|
(View with Genome Compiler)
|Promoter:||Chicken β-actin/rabbit β-globin hybrid promoter (AG)
Human cytomegalovirus immediate early promoter (CMV-IE); enhancer only
Escherichia coli lac operon promoter
|Terminator:||Rabbit β-globin polyadenylation signal (β-globin polyA)
Simian virus 40 polyadenylation signal (SV40 polyA)
|Selection marker:||Ampicillin (amp)|
|Replicon:||Escherichia coli plasmid pMB1 origin
Simian virus 40 bidirectional origin (SV40)
|Host range:||Escherichia coli
Mammalian cells; SV40 permissive cells
|Parental clone:||pUC13; pAGS-3|
|Further information:||This plasmid is useful for highly efficient expression of genes under the control of the AG promoter and the human CMV-IE enhancer in various mammalian cells.
The AG promoter sequence consists of the chicken β-actin promoter, the first exon and part of the first intron (that seems to have a strong enhancer-like activity) linked to a rabbit β-globin fragment, consisting of a 3' part of the second intron (inclusive a branch point which is required for normal splicing reactions) and a 5' part of the third exon.
When cloning a fragment downstream from the lac promoter it may be advisable to use lacI(q) strains in order to prevent fortuitous expression of a possibly noxious polypeptide.
The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number LT727518.1 except for the adjustments due to the implementation of the next-generation sequencing results.
|EMBL Accession number:||LT727518.1, view at EMBL, GenBank, DDBJ|
|Latest sequence update:||05/11/2019|
|Authenticity test:||Restriction enzyme pattern analysed at GeneCorner: BglII, EcoRI, NcoI, SacII and XhoI.
This plasmid has also been fully sequenced but the NGS sequence data were unfortunately incomplete.
|History of deposit:||This plasmid was deposited by Prof. Dr J. Miyazaki(1).
(1) University of Tokyo, Japan
|Plasmid reference:||Niwa et al., Gene 108 (1991), 193-200 [PMID: 1660837]
|Related plasmid reference:||Staal et al. (2017) [DOI: 10.1101/228767]
Verhelst et al., EMBO J. 31 (2012), 3845-3855 [PMID: 23032186] [DOI: 10.1038/emboj.2012.240]
Thorrez et al., Mol. Ther. 14 (2006), 442-451 [PMID: 16750937] [DOI: 10.1016/j.ymthe.2006.03.019]
Pattabriaman et al., J. Cell Biol. 208 (2015), 53-69 [PMID: 25547156] [DOI: 10.1083/jcb.201404109]
Lee et al., Mol. Cancer 10 (2011), 113 [PMID: 21917150] [DOI: 10.1186/1476-4598-10-113]
|Restricted distribution:||- BCCM MTA|
|Distributed as:||H/P active culture or plasmid DNA|
|Host for distribution:||Escherichia coli K12 MC1061 (LMBP 09560)|
|Host reference:||Casadaban et al., J. Mol. Biol. 138 (1980), 179-207 [PMID: 6997493]
|Related host reference:||Brigé et al., Biochem. J. 394 (2006), 335-344 [PMID: 16293111]
|Cultivation medium:||LB-Lennox + ampicillin (100 μg/ml)|
|Other culture collection numbers:||-|
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.