Last data update: 24 January 2024 16:39 CET
Plasmid name: pACYC184 (LMBP 992)
| New search | Print data sheet |
| Price category: | Cat. 1 (cf. price list) |
| Status: | GeneCorner core plasmid |
| GeneCorner sequence: |
p992.gb
(View with Genome Compiler) p992.txt p992.pdf |
| Sequence analysis results Genecorner: |
- |
| Cloned DNA: |
- |
| Promoter: | - |
| Ribosome binding site: |
- |
| Terminator: | - |
| Selection marker: | Chloramphenicol (cam) Tetracycline (tet) |
| Replicon: | Escherichia coli plasmid p15A origin |
| Copy number: | Low copy number |
| Host range: | Escherichia coli |
| Parental clone: | p15A; pSC101; pKT002 |
| Further information: | This multicopy plasmid is compatible with ColE1- or pMB1-derived plasmids. pACYC184 can be amplified 10-fold in the presence of spectinomycin. The chloramphenicol resistance gene is excisable as a HaeII fragment. The nucleotide sequence of this plasmid corresponds with the EMBL Nucleotide Sequence Database accession number X06403.1. |
| EMBL Accession number: | X06403.1, view at EMBL, GenBank, DDBJ |
| Latest sequence update: | 28/10/1994 |
| Authenticity test: | Restriction enzyme pattern analysed at GeneCorner: BamHI/HindIII, EcoRI/XmnI, HindIII and RsaI. |
| Class: | Recombinant plasmid |
| Type: | Plasmid |
| History of deposit: | This plasmid was deposited by Dr S. Cohen(1). (1) Stanford University School of Medicine, USA |
| Plasmid reference: | Chang et al., J. Bacteriol. 134 (1978), 1141-1156 [PMID: 149110] |
| Restricted distribution: | - BCCM MTA |
| Distributed as: | H/P active culture or plasmid DNA |
| Host for distribution: | Escherichia coli K12 K514(λ) |
| Host reference: | Zabeau et al., EMBO J. 1 (1982), 1217-1224 [PMID: 6327257] |
| Cultivation medium: | LB-Lennox + chloramphenicol (25 μg/ml) + tetracycline (10 μg/ml)* |
| Cultivation temperature: | 37°C |
| Biosafety level: | L1 |
| Cultivation remark: | *: selection of transformants on chloramphenicol; subsequent cultivation of a single colony in liquid medium with tetracycline. |
| Other culture collection numbers: | DSM 4439 ATCC 37033 NCIB 12392 NRRL B-14221 NCCB 3011 PC-V 3010 |
Refer in your Materials and Methods: |
pACYC184 (LMBP 992) is available at BCCM/GeneCorner. This plasmid was deposited by Dr S. Cohen and was published in Chang et al., 1978. |
Note: Up-to-date, validated data are enclosed with the biological material. Nevertheless, these data are a snapshot at a given moment; further updates are always possible.