Last data update:
24 January 2024 16:39 CET
1 medium found
| Name: | YCFA medium |
| Medium number: | 1034 |
| Recipe: | YCFA Medium Before Autoclaving Optional agar 15 g Tryptone 10 g Yeast extract 2.5 g NaHCO3 4.0 g (D)+Glucose 2.0 g (D)+Maltose 2.0 g (D)+Cellobiose 2.0 g L-cysteine 1.0 g Mineral Solution I 150 ml Mineral Solution II 150 ml Resazurin Solution 1.0 ml Haemin Solution 10 ml Vitamin solution I 1.0 ml d.H2O up to 1L VFA mix 6.2 ml NaOH Adjust pH to 7.45 After Autoclaving Vitamin solution II 1.0 ml Resazurin Solution Resazurin 0.1g d.H2O 100ml Mineral Solution I: K2HPO4 3g d.H2O 1L Mineral Solution II: KH2PO4 3g (NH4)2SO4 6g NaCl 6g MgSO4 0.6g CaCl2 (dry) 0.6g d.H2O 1L VFA mix: Acetic acid 17ml Propionic acid 6ml n-Valeric acid 1ml Isovaleric acid 1ml Isobutyric acid 1ml Haemin Solution: KOH 0.28g Ethanol 95% 25ml Haemin 0.1g d.H2O up to 100 ml Vitamin Solution I: Biotin 5mg Cobalamin (Vitamin B12) 5mg PABA (4-Aminobenzoic Acid) 15mg Folic acid 25mg Pyridoxine 75mg d.H2O up to 500ml Vitamin Solution II: Thiamine hydrochloride 25mg Riboflavin 25mg d.H2O up to 500ml |