Last data update:
24 January 2024 16:39 CET
1 medium found
| Name: | Medium for Tundrispaera lichenicola |
| Medium number: | 1037 |
| Recipe: | 0.1 g KH2PO4 0.1g (NH4)2SO4 10 ml Mineral salt sol. (Hutner/Basal Salt) 0.5 g N-acetylglucosamine 0.25 g glucose 0.2 g yeast extract Vitamin solution N°6 (Staley, 1968) pH 5.8-6.2 Vitamin solution N°6 (Staley, 1968) Biotin 4 mg Pyridoxine hydrochloride 20 mg Thiamin hydrochloride 10 mg Ca pantothenate 10 mg p-Aminobenzoic acid 10 mg Folic acid 4 mg Riboflavin 10 mg Nicotinic acid or nicotinamide 10 mg Vitamin B12 0,2 mg Distilled water 1 L Sterilze by filtration only. Mineral salt sol. (Hutner/Basal Salt): NTA 10 g MgSO4 x 7H2O 29,70 g CaCl2 x 2H2O 3,34 g Na2MoO4 x 2H2O 12,67 mg FeSO4 x 7H2O 99 mg Metall salt sol. "44" 50 ml Distilled water add to 1L Dissolve NTA in 700 ml distill water by adjusting pH to 7.2 with KOH. Dissolve all components separately and combine slowly. Sterilize by filtration. Store at 4°C "Metals 44": Na-EDTA 250 mg ZnSO4 x 7H2O 1095 mg FeSO4 x 7H2O 500 mg MnSO4 x H2O 154 mg CuSO4 x 5H2O 39,5 mg CoCl2 x 6H2O 20,3 mg Na2B4O7 x 10H2O 17,7 mg Distilled water 1L Dissolve the EDTA in 700 ml and add a few drops of concentrated H2SO4 to retard precipitation of the heavy metals if necessary. Store at 4°C. Do not autoclave. |