Last data update:
24 January 2024 16:39 CET
1 medium found
Name: | |
Medium number: | 137 |
Recipe: | NH4Cl 1 g MgCl2 0,5 g MgSO4 0,3 g KH2PO4 0,4 g K2HPO4 0,6 g FeCl3 20 mg Na2S2O3 10 g Brom thymol blue 30 mg Heavy Metal Solution 30 ml (see below) Yeast extract 5,0 g Distilled water 1,0 L pH 6,8 Heavy Metal Solution: Ethylene diamine tetraacetate (EDTA should be dissolved first) 1,5 g Trace Element Solution (Hoagland) (see below) 6 ml FeSO4 x 7H2O 0,2 g ZnSO4 x H2O 0,1 g MnCl2 x 4H2O 0,02 g Distilled water 1 L Modified Hoagland Trace Element Solution: AlCl3 1 g Kl 1 g KBr 0,5 g LiCl 0,5 g MnCl2 x 4H2O 7 g H3BO3 11 g ZnCl2 1 g CuCl2 1 g NiCl2 1 g CoCl2 1 g SnCL2 x 2H2O 0,5 g BaCl2 0,5 g Na2MoO4 0,5 g NaVO3 x H2O 0,1 g Se-salt 0,5 g Dissolve each salt in distilled water before mixing. Adjust the pH of each solution to below 7,0. Adjust the final pH to 3-4. The total final volume is 3.6 liters. The flaky yellow precipitate which is formed after mixing transforms after a few days into a very fine white precipitate. Mix the solution thoroughly before use. |