Last data update:
24 January 2024 16:39 CET
1 medium found
| Name: | RAE Medium (Reinforced AE Medium) |
| Medium number: | 239 |
| Recipe: | Glucose 40 g Yeast extract 10 g Peptone 10 g Na2HPO4 x 12H2O 3,38 g Citric acid x H2O 1,5 g Ethanol 20 ml Acetic acid 10 ml Distilled water 1 L The ethanol and acetic acid are sterilized separately by filtration and added aseptically to the sterile medium. A double layer system has to be used. A layer of growth medium containing 1% agar is poured into a plate and allowed to solidify. A thin layer of the growth medium containing 2% agar is then poured on top. |