GREAT AT SMALL THINGS

LMG media details

Last data update: 25 March 2019 04:20 CET  

1 medium found

Name:

Medium number: 296

Recipe: K2HPO4 0,8 g

KH2PO4 0,2 g

MgSO4 x 7H2O 0,5 g

FeSO4 x 7H2O 0,01 g

(NH4)2SO4 1 g

Trace element solution 1 ml

see below

Vitamin solution 5 ml

see below

Agar 15 g

Distilled water 1 L

Add 500 ┬Ál of hexane (C-source) into the lid of a Petri-dish containg this medium. The strain must grow in the vapour without direct contact with the cells.

Trace element solution:

Na2 EDTA 3 g

MnCl2 x 2H2O 0,05 g

CoCl2 x 6H2O 0,19 g

ZnCl2 0,041 g

H3BO3 0,006 g

NiCl2 x 6H2O 0,024 g

CuCl2 0,002 g

Na2MoO4 x 2H2O 0,018 g

Distilled water 1 L

Adjust pH to 6.

Vitamin solution:

Thiamine 0,01 g

Nicotinic acid 0,02 g

Pyridoxine hydrochloride 0,02 g

p -Aminobenzoic acid 0,01 g

Riboflavin 0,02 g

Panthotein acid 0,02 g

Biotin 0,001 g

Cyanocobalamin 0,001 g

Distilled water 1 L

Adjust pH to 7.