GREAT AT SMALL THINGS

LMG media details

Last data update: 15 July 2019 04:12 CEST  

1 medium found

Name: Thiomonas medium:

Medium number: 299

Recipe: Solution A:

K2HPO4 0,5 g

KH2PO4 0,5 g

NaCl 0,5 g

(NH4)2SO4 0,05 g

Yeast extract 0,2 g

Trace element solution 1 ml

see below

Agar 15 g

Distilled water 500 ml

Adjust the pH to 6 with H2SO 4.

Solution B:

CaCl2 0,1 g

MgSO4 x 7H2O 0,1 g

Distilled water 500 ml

Sterilize solution A and solution B by autoclaving. After cooling, mix solutions A and B.

Trace element solution:

HCl 25% 6,5 ml

FeCl2 1,5 g

H3BO3 60 mg

MnSO4 x H2O 117 mg

CoCl2 x 6H2O 25 mg

ZnCl2 70 mg

NiCl2 x 6H2O 25 mg

CuCl2 x 2H2O 15 mg

Na2MoO4 x 2H2O 25 mg

Distilled water 1 L