Last data update:
24 January 2024 16:39 CET
1 medium found
| Name: | ABM2 (Alkaline Buffered medium 2) |
| Medium number: | 316 |
| Recipe: | Yeast extract 5 g Triptone 5 g Alpha-ketoglutaric acid monopotassium salt (Sigma) 1 g Macronutrients solution 10x 100 ml Micronutrients solution 100x 10 ml Carbonate-bicarbonate buffer solution 1 M pH 9 100 ml Agar 15 g Distilled water up to 1 L Adjust pH to 9 with NaOH. Autoclave at 121°C for 15 minutes. Macronutrients solution 10x: Nitrilotriacetic acid 1 g CaSO4 x 2H2O 0,6 g MgSO4 x 7H2O 1 g NaCl 0,8 g KNO3 1,03 g NaNO3 6,89 g Na2HPO4 1,11 g Distilled water 1 L Micronutrients solution 100x: MnSO4 x H2O 220 mg ZnSO4 x 7H2O 50 mg H3BO3 50 mg CuSO4 x 5H2O 2,5 mg Na2MoO4 x 2H2O 2,5 mg CoCl2 x 6H2O 4,6 mg Distilled water 1 L |