GREAT AT SMALL THINGS

0

LMG media details

Last data update: 24 January 2024 16:39 CET  

1 medium found

Name: Modified R3 medium

Medium number: 317

Recipe: Yeast extract 1 g

Proteose Peptone 1 g

Casamino Acids 1 g

Glucose 1 g

K2HPO4 0,6 g

MgSO4 x 7H2O 0,1 g

Pyruvate 0,5 g

Macronutrients

solution 10x 50 ml

Micronutrients

solution 100x 5 ml

Tris buffer solution 1 M pH 8 100 ml

Agar 15 g

Distilled water up to 1 L

Adjust pH to 8 with NaOH.

Autoclave at 121°C for 15 minutes.

Thermus macronutrients solution 10x:

Nitrilotriacetic acid 1 g

CaSO4 x 2H2O 0,6 g

MgSO4 x 7H2O 1 g

NaCl 0,8 g

KNO3 1,03 g

NaNO3 6,89 g

Na2HPO4 1,11 g

Distilled water 1 L

Thermus micronutrients solution 100x:

MnSO4 x H2O 220 mg

ZnSO4 x 7H2O 50 mg

H3BO3 50 mg

CuSO4 x 5H2O 2,5 mg

Na2MoO4 x 2H2O 2,5 mg

CoCl2 x 6H2O 4,6 mg

Distilled water 1 L