Last data update:
24 January 2024 16:39 CET
1 medium found
Name: | Modified R3 medium |
Medium number: | 317 |
Recipe: | Yeast extract 1 g Proteose Peptone 1 g Casamino Acids 1 g Glucose 1 g K2HPO4 0,6 g MgSO4 x 7H2O 0,1 g Pyruvate 0,5 g Macronutrients solution 10x 50 ml Micronutrients solution 100x 5 ml Tris buffer solution 1 M pH 8 100 ml Agar 15 g Distilled water up to 1 L Adjust pH to 8 with NaOH. Autoclave at 121°C for 15 minutes. Thermus macronutrients solution 10x: Nitrilotriacetic acid 1 g CaSO4 x 2H2O 0,6 g MgSO4 x 7H2O 1 g NaCl 0,8 g KNO3 1,03 g NaNO3 6,89 g Na2HPO4 1,11 g Distilled water 1 L Thermus micronutrients solution 100x: MnSO4 x H2O 220 mg ZnSO4 x 7H2O 50 mg H3BO3 50 mg CuSO4 x 5H2O 2,5 mg Na2MoO4 x 2H2O 2,5 mg CoCl2 x 6H2O 4,6 mg Distilled water 1 L |