GREAT AT SMALL THINGS

LMG media details

Last data update: 25 April 2019 04:11 CEST  

1 medium found

Name:

Medium number: 33

Recipe: Peptone 5 g

Casitone 5 g

Yeast extract 10 g

(NH4)2SO4 0,5 g

Glucose 5 g

Resazurin 1 mg

Mineral solution (see below) 40 ml

Fatty acid mixture 3,1 ml

(see below)

Distilled water up to 1 L

Mineral solution:

CaCl2 x 2H2O 0,3 g

MgSO4 x 7H2O 0,48 g

K2HPO4 1 g

KH2PO4 1 g

NaHCO3 10 g

NaCl 2 g

Distilled water 1 L

Fatty acid mixture:

Acetic acid 17 ml

Propionic acid 6 ml

n -Butyric acid 4 ml

n -Valeric acid 1 ml

iso -Valeric acid 1 ml

iso -Butyric acid 1 ml

DL-2-Methylbutyric acid 1 ml

Dissolve ingredients, boil for 5 minutes, cool to room temperature while gassing with 100% CO2, and add 5 mg haemin (dissolved in 0,5 ml 1N NaOH), 0,2 ml vitamin K1 and 0,5 g cysteine hydrochloride per liter medium. Adjust pH to 6,9 with 8N NaOH while gassing is continued. Switch to 100% N2 gas after pH has reached the desired value, distribute in anaerobic tubes under N2 and sterilize. If needed, add 15 g agar per liter medium.