GREAT AT SMALL THINGS

LMG media details

Last data update: 24 May 2019 04:16 CEST  

1 medium found

Name: SSE/HD1:10, pH 7

Medium number: 429

Recipe: Solution A:

Peptone 0,5 g

Yeast extract 0,25 g

Glucose 0,1 g

HEPES (10mM concentration) 1,95 g

SSE (2x) (see below) 500 ml

Adjust pH to 7 autoclave

Solution B:

Agar (purified) 15 g

Distilled water 500 ml

Do not adjust pH, autoclave

After autoclaving, let cool solution A and B to 55°C, combine and add 1ml SL10 and 1 ml 10-vitamins-solution (100x) (see below)



SSE (2x)

CaCl2 x 2H2O 0,2938 g

NH4Cl 0,1069 g

MgCl2 x 6H2O 0,2036 g

(NH4) 2SO4 0,1983 g

MgSO4 x 7H2O 0,7390 g

CaSO4 x 2H2O 0,8606 g

Ca(NO3)2 x 4H2O 0,2360 g

NaNO3 0,4240 g

KH2PO4 (100mM solution) 0,5 ml

FeSO4 x 7H2O 0,0111 g

K2SO4 0,0870 g

Distilled water 1000 ml

Stir for minimum 5h and autoclave in case of no direct use (storage).

SL10

Hydrochloric acid (25%) 10 ml

FeCl2 x 4H2O 1,5 g

CoCl2 x 6H2O 0,190 g

MnCl2 x 2H2O 0,100 g

ZnCl2 0,070 g

NiCl2 x 6H2O 0,024 g

Na2MoO4 x 2H2O 0,036 g

H3BO3 0,006 g

CuCl2 x 2H2O 0,002 g

Distilled water 1000 ml

First solve FeCl2 in hydrochloric acid, add some water and the other components, ajust to 1L. Do not adjust pH (is about 1,15), filter sterilize.

10-vitamins-solution (100x)

Biotin 2 mg

Folic acid 2 mg

Pyridoxinhydrochloride 10 mg

Thiaminhydrochloride 5 mg

Riboflavin 5 mg

Nicotinic acid 5 mg

DL-calcium pantothenate 5 mg

Vitamin B12 0,1 mg

p-aminobenzoic acid 5 mg

Lipoic acid 5 mg

Distilled water 1000 ml

Filter sterilize