Last data update:
24 January 2024 16:39 CET
1 medium found
| Name: | PYG medium (modifed) |
| Medium number: | 454 |
| Recipe: | Trypticase peptone 5 g Peptone 5 g Yeast extract 10 g Beef extract 5 g Glucose 5 g K2HPO4 2 g Tween 80 1 ml Cysteine-HCl x H2O 0,5 g Resazurin 1 mg Salt solution (see below) 40 ml Distilled water 950 ml Haemin solution (see below) 10 ml Vitamin K1 solution (see below) 0,2 ml The vitamin K1, haemin and cysteine are added after the medium has been boiled and cooled under N2. Adjust to pH 7,2 using NaOH. Distribute under N2 and autoclave. Salt solution: CaCl2 x 2H2O 0,25 g MgSO4 x 7H2O 0,50 g K2HPO4 1 g KH2PO4 1 g NaHCO3 10 g NaCl 2 g Distilled water 1 L Haemin solution: Dissolve 50 mg haemin in 1 ml NaOH, make up to 100 ml with distilled water. Store refrigerated. Vitamin K1 solution: Dissolve 0,1 ml of vitamin K1 in 20 ml 95% ethanol and filter sterilize. Store refrigerated in a brown bottle. For solid medium add 4 g gellan gum per 500 ml medium |