GREAT AT SMALL THINGS

LMG media details

Last data update: 15 November 2019 04:07 CET  

1 medium found

Name:

Medium number: 60

Recipe: MgSO4 x 7H2O 0,1 g

KNO3 0,1 g

CaCl2 x 2H2O 0,1 g

Sodium-alpha-

glycerophosphate 0,1 g

Tris buffer 1 g

Thiamine 1 mg

Casamino acids (Difco 0230) 1 g

Cobalamin (= vitamin B12) 1 µg

Trace element solution 1 ml

(see below)

Agar 15 g

Distilled water up to 1 L

pH 7,5

Trace element solution:

H3BO3 2,85 g

MnCl2 x 4H2O 1,8 g

FeSO4 x 7H2O 2,49 g

CuCl2 x 2H2O 26,9 mg

ZnCl2 20,8 mg

CoCl2 x 6H2O 40,4 mg

Na2MoO4 x 2H2O 25,2 mg

Sodium tartrate 1,77 g

Distilled water 1 L

After sterilization, aseptically add 10 ml of a filter-sterilized 10% glucose solution per liter medium.