GREAT AT SMALL THINGS

LMG media details

Last data update: 25 April 2019 04:11 CEST  

1 medium found

Name: ISP Medium 4 (Difco 0772)

Medium number: 92

Recipe: Soluble starch 10 g

(NH4)2SO4 2 g

MgSO4 x 7H2O 1 g

K2HPO4 1 g

NaCl 1 g

CaCO3 2 g

Trace salts solution 1 ml

(see below)

Agar 20 g

Distilled water 1 L

pH 7,0-7,4

Trace salts solution:

FeSO4 x 7H2O 0,1 g

MnCl2 x 4H2O 0,1 g

ZnSO4 x 7H2O 0,1 g

Distilled water 100 ml

Make a paste of the starch with a small amount of cold distilled water and bring to a volume of one liter, then add other ingredients.