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Antifungal susceptibility testing

BCCM/IHEM performs antifungal susceptibility testing following the EUCAST (European Committee on Antimicrobial Susceptibility Testing) guidelines. The method utilizes broth microdilution plates to determine the minimum inhibitory concentration (MIC) values for a series of antifungal drugs.

Tested drugs are:

  1. For yeasts: amphotericin B, fluconazole, itraconazole, voriconazole, caspofungin, micafungin and anidulafungin
  2. For moulds: amphotericin B, fluconazole, itraconazole, voriconazole, ketoconazole, flucytosine and terbinafine
  3. For dermatophytes: fluconazole, itraconazole, voriconazole, ketoconazole, terbinafine, griseofulvin and ciclopirox olamine

If available, breakpoint values provided by the EUCAST can classify strains as susceptible or resistant. If these breakpoint values are not available, MIC values are compared to data published in the scientific literature in order to suggest the susceptible/resistant status of the strain.

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