GREAT AT SMALL THINGS

BCCM Training 2018 : PROGRAM

The preservation of micro-organisms: a practical approach

Management of a culture collection: seminar

 

PROGRAM

 

DAY 1-3 (18-20 September) : 

3 days training on the handling and preservation of an organism type of your choice 

 

Bacteria (up to biosafety level 2Q)

Topics:

  • General Practices for the handling of Bacteria
    • Culturing, isolation and purity check
    • Working under ISO9001
  • Freeze drying
    • Concept and overview
    • Residual Moisture Content determination 
    • Hands on training
    • Data management
  • Cryopreservation
    • Concept and overview
    • Hands on training
    • Data management
  • Safeguarding stricktly anaerobic bacterial cultures
    • Concept and over
  • Encapsulation dehydratation vitrification
    • Concept and overview
  • DNA bank
    • Concept and overview
  • Viability
    • Methods for viability assessement
  • General discussion
    • Question and Answer session

 

or

 

Bacteria (biosafety level 3, specialised in Mycobacteria)

Topics:

  • General Practices for the handling of Bacteria
    • Culturing, isolation and purity check
    • Working under ISO9001
  • Freeze drying
    • Concept and overview
    • RMC determination (theory)
    • Hands on training
    • Data management
  • Viability & purity checks
    • Methods for viability & purity assessment
    • Data management
  • Cryopreservation (liquid nitrogen)
    • Concept and overview
    • Demonstration
  • Cryopreservation (-80°C)

    • Concept and overview
    • Hands-on training
    • Data management
  • DNA-bank
    • Concept and overview
  • General discussion
    • Question and Answer session

 

or

 

Cyanobacteria

Topics:

  • General Practices for the handling of Cyanobacteria
    • Working under ISO9001
  • Microscopical observations and taxonomy
    • Hands on training
  • Culturing and culture media
    • Sterile transfers for preservation of living cultures
    • Hands on training
  • Isolation from environmental samples and purification
    • Controls of purity
    • Hands on training
  • Cryopreservation
    • Concept and overview
    • Hands on training
  • DNA bank
    • Concept and overview
  • Round Table discussion

 

or

 

Fungi

Topics:

  • General Good Laboratory Practices for the handling of Fungi
    • Culture
    • Culture media
    • Isolation
  • Freeze drying
    • Concept and overview
    • Hands on training
  • Cryopreservation
    • Concept and overview
    • Hands on training
  • Cryopreservation on cryobeads
    • Concept and overview
    • Hands on training
  • Preservation under water or oil
    • Concept and overview
    • Hands on training
  • General discussion
    • Question and Answer session
    • New developments and future perspectives

 

or

 

Microalgae (specialised in diatoms)

Topics:

  • General Good Laboratory Practices for the handling of Microalgae
    • Isolation
    • Culture media
    • Culture
  • Stock cultures
    • Concept and overview
    • Hands on training
  • Diatom life cycle
    • Concept and overview
    • Hands on training
  • Cryopreservation and thawing
    • Concept and overview
    • Hands on training
  • General discussion
    • Question and Answer session
    • Future perspectives

 

Day 4 (21 September) :

Option 1 :  one-day initiation on the handling and preservation of another organism type of your choice (same topics as for day 1-3 but less elaborated and through demo's)

Bacteria

or

Cyanobacteria

or

Fungi

or

Microalgae

 

Option 2 : one-day seminar on 'Management of a culture collection'

All presentations will be given in English.

  • Information management

  • Quality management

  • Shipment

  • Nagoya protocol

  • Presentation of the services of BCCM/GeneCorner

 

SOCIAL EVENT

On Wednesday 19th of September a social event and a dinner will be organised (included in the training fee for day 1-3).

 

Download the registration form for the BCCM training