The Standardized Genome Architecture platform allows for predictable expression levels and rapid interchange of functional modules in Escherichia coli. These well-characterized modules were optimized for robust performance in the context of the bacterial genome.
SEGA makes genomic engineering of E. coli extremely accessible, even if you have no prior lab experience. In its simplest form, only two reagents are required for genome engineering: a (synthetic) DNA fragment and a stock solution of bacterial SEGA cells.
The SEGA landing pad
The SEGA strains were published by Bayer et al. (2021). More info can also be found on sega-genomes.com.
And indeed, SEGA was inspired by the SEVA plasmids initiative, of which the most important plasmids are also available at BCCM/GeneCorner. Check out the SEVA plasmids as well!
|
You can order strains individually, order the entire SEGA set, or select one of the subsets at a strong discount (see price list). |
Add the entire SEGA set to your shopping cart | |
| Strain information can be downloaded for the entire set: | Sequence files (GenBank) |
| Subset "Untagged expression" | Subset "Building new functions" | Subset "J23100" | Subset "PTrc" | Subset "PRha" | Subset "T7" |
| SEGA N° | Accession N° | E. coli strain | Locus | Upstream gadget |
C1 | C2 | Middle gadget |
Cargo | Downstream gadget |
|---|---|---|---|---|---|---|---|---|---|
| SEGA001 | LMBP 12837 | MG1655 | glmS-pstS | none | J23100 | none | none | none | 3'tetA |
| SEGA002 | LMBP 12838 | MG1655 | glmS-pstS | none | Ptrc | none | none | none | 3'tetA |
| SEGA003 | LMBP 12839 | MG1655 | glmS-pstS | none | PrhaBAD | none | none | none | 3'tetA |
| SEGA004 | LMBP 12840 | MG1655(DE3) | glmS-pstS | none | PT7 | none | none | none | 3'tetA |
| SEGA005 | LMBP 12841 | MG1655 | glmS-pstS | none | J23100 | low | BCD | gfp | tetA |
| SEGA006 | LMBP 12842 | MG1655 | glmS-pstS | none | J23100 | medium | BCD | gfp | tetA |
| SEGA007 | LMBP 12843 | MG1655 | glmS-pstS | none | J23100 | high | BCD | gfp | tetA |
| SEGA008 | LMBP 12844 | MG1655 | glmS-pstS | none | J23100 | low | Ubiquitin | gfp | tetA |
| SEGA009 | LMBP 12845 | MG1655 | glmS-pstS | none | J23100 | medium | Ubiquitin | gfp | tetA |
| SEGA010 | LMBP 12846 | MG1655 | glmS-pstS | none | J23100 | high | Ubiquitin | gfp | tetA |
| SEGA011 | LMBP 12847 | MG1655 | glmS-pstS | none | J23100 | low | TEV | gfp | tetA |
| SEGA012 | LMBP 12848 | MG1655 | glmS-pstS | none | J23100 | medium | TEV | gfp | tetA |
| SEGA013 | LMBP 12849 | MG1655 | glmS-pstS | none | J23100 | high | TEV | gfp | tetA |
| SEGA014 | LMBP 12850 | MG1655 | glmS-pstS | none | J23100 | low | PROTi | gfp | tetA |
| SEGA015 | LMBP 12851 | MG1655 | glmS-pstS | none | J23100 | medium | PROTi | gfp | tetA |
| SEGA016 | LMBP 12852 | MG1655 | glmS-pstS | none | J23100 | high | PROTi | gfp | tetA |
| SEGA017 | LMBP 12853 | MG1655 | glmS-pstS | none | J23100 | low | PelBss | sfgfp | tetA |
| SEGA018 | LMBP 12854 | MG1655 | glmS-pstS | none | J23100 | medium | PelBss | sfgfp | tetA |
| SEGA019 | LMBP 12855 | MG1655 | glmS-pstS | none | J23100 | high | PelBss | sfgfp | tetA |
| SEGA020 | LMBP 12856 | MG1655 | glmS-pstS | none | Ptrc | low | BCD | gfp | tetA |
| SEGA021 | LMBP 12857 | MG1655 | glmS-pstS | none | Ptrc | medium | BCD | gfp | tetA |
| SEGA022 | LMBP 12858 | MG1655 | glmS-pstS | none | Ptrc | high | BCD | gfp | tetA |
| SEGA023 | LMBP 12859 | MG1655 | glmS-pstS | none | Ptrc | low | Ubiquitin | gfp | tetA |
| SEGA024 | LMBP 12860 | MG1655 | glmS-pstS | none | Ptrc | medium | Ubiquitin | gfp | tetA |
| SEGA025 | LMBP 12861 | MG1655 | glmS-pstS | none | Ptrc | high | Ubiquitin | gfp | tetA |
| SEGA026 | LMBP 12862 | MG1655 | glmS-pstS | none | Ptrc | low | TEV | gfp | tetA |
| SEGA027 | LMBP 12863 | MG1655 | glmS-pstS | none | Ptrc | medium | TEV | gfp | tetA |
| SEGA028 | LMBP 12864 | MG1655 | glmS-pstS | none | Ptrc | high | TEV | gfp | tetA |
| SEGA029 | LMBP 12865 | MG1655 | glmS-pstS | none | Ptrc | low | PROTi | gfp | tetA |
| SEGA030 | LMBP 12866 | MG1655 | glmS-pstS | none | Ptrc | medium | PROTi | gfp | tetA |
| SEGA031 | LMBP 12867 | MG1655 | glmS-pstS | none | Ptrc | high | PROTi | gfp | tetA |
| SEGA032 | LMBP 12868 | MG1655 | glmS-pstS | none | Ptrc | low | PelBss | sfgfp | tetA |
| SEGA033 | LMBP 12869 | MG1655 | glmS-pstS | none | Ptrc | medium | PelBss | sfgfp | tetA |
| SEGA034 | LMBP 12870 | MG1655 | glmS-pstS | none | Ptrc | high | PelBss | sfgfp | tetA |
| SEGA035 | LMBP 12871 | MG1655 | glmS-pstS | none | Ptrc | low | YebF | sfgfp | tetA |
| SEGA036 | LMBP 12872 | MG1655 | glmS-pstS | none | Ptrc | medium | YebF | sfgfp | tetA |
| SEGA037 | LMBP 12873 | MG1655 | glmS-pstS | none | Ptrc | high | YebF | sfgfp | tetA |
| SEGA038 | LMBP 12874 | MG1655 | glmS-pstS | none | PrhaBAD | low | BCD | gfp | tetA |
| SEGA039 | LMBP 12875 | MG1655 | glmS-pstS | none | PrhaBAD | medium | BCD | gfp | tetA |
| SEGA040 | LMBP 12876 | MG1655 | glmS-pstS | none | PrhaBAD | high | BCD | gfp | tetA |
| SEGA041 | LMBP 12877 | MG1655 | glmS-pstS | none | PrhaBAD | low | Ubiquitin | gfp | tetA |
| SEGA042 | LMBP 12878 | MG1655 | glmS-pstS | none | PrhaBAD | medium | Ubiquitin | gfp | tetA |
| SEGA043 | LMBP 12879 | MG1655 | glmS-pstS | none | PrhaBAD | high | Ubiquitin | gfp | tetA |
| SEGA044 | LMBP 12880 | MG1655 | glmS-pstS | none | PrhaBAD | low | TEV | gfp | tetA |
| SEGA045 | LMBP 12881 | MG1655 | glmS-pstS | none | PrhaBAD | medium | TEV | gfp | tetA |
| SEGA046 | LMBP 12882 | MG1655 | glmS-pstS | none | PrhaBAD | high | TEV | gfp | tetA |
| SEGA047 | LMBP 12883 | MG1655 | glmS-pstS | none | PrhaBAD | low | PROTi | gfp | tetA |
| SEGA048 | LMBP 12884 | MG1655 | glmS-pstS | none | PrhaBAD | medium | PROTi | gfp | tetA |
| SEGA049 | LMBP 12885 | MG1655 | glmS-pstS | none | PrhaBAD | high | PROTi | gfp | tetA |
| SEGA050 | LMBP 12886 | MG1655 | glmS-pstS | none | PrhaBAD | low | PelBss | sfgfp | tetA |
| SEGA051 | LMBP 12887 | MG1655 | glmS-pstS | none | PrhaBAD | medium | PelBss | sfgfp | tetA |
| SEGA052 | LMBP 12888 | MG1655 | glmS-pstS | none | PrhaBAD | high | PelBss | sfgfp | tetA |
| SEGA053 | LMBP 12889 | MG1655 | glmS-pstS | none | PrhaBAD | low | YebF | sfgfp | tetA |
| SEGA054 | LMBP 12890 | MG1655 | glmS-pstS | none | PrhaBAD | medium | YebF | sfgfp | tetA |
| SEGA055 | LMBP 12891 | MG1655 | glmS-pstS | none | PrhaBAD | high | YebF | sfgfp | tetA |
| SEGA056 | LMBP 12892 | MG1655(DE3) | glmS-pstS | none | PT7 | low | BCD | gfp | tetA |
| SEGA057 | LMBP 12893 | MG1655(DE3) | glmS-pstS | none | PT7 | medium | BCD | gfp | tetA |
| SEGA058 | LMBP 12894 | MG1655(DE3) | glmS-pstS | none | PT7 | high | BCD | gfp | tetA |
| SEGA059 | LMBP 12895 | MG1655(DE3) | glmS-pstS | none | PT7 | low | Ubiquitin | gfp | tetA |
| SEGA060 | LMBP 12896 | MG1655(DE3) | glmS-pstS | none | PT7 | medium | Ubiquitin | gfp | tetA |
| SEGA061 | LMBP 12897 | MG1655(DE3) | glmS-pstS | none | PT7 | high | Ubiquitin | gfp | tetA |
| SEGA062 | LMBP 12898 | MG1655(DE3) | glmS-pstS | none | PT7 | low | TEV | gfp | tetA |
| SEGA063 | LMBP 12899 | MG1655(DE3) | glmS-pstS | none | PT7 | medium | TEV | gfp | tetA |
| SEGA064 | LMBP 12900 | MG1655(DE3) | glmS-pstS | none | PT7 | high | TEV | gfp | tetA |
| SEGA065 | LMBP 12901 | MG1655(DE3) | glmS-pstS | none | PT7 | low | PROTi | gfp | tetA |
| SEGA066 | LMBP 12902 | MG1655(DE3) | glmS-pstS | none | PT7 | medium | PROTi | gfp | tetA |
| SEGA067 | LMBP 12903 | MG1655(DE3) | glmS-pstS | none | PT7 | high | PROTi | gfp | tetA |
| SEGA068 | LMBP 12904 | MG1655(DE3) | glmS-pstS | none | PT7 | low | PelBss | sfgfp | tetA |
| SEGA069 | LMBP 12905 | MG1655(DE3) | glmS-pstS | none | PT7 | medium | PelBss | sfgfp | tetA |
| SEGA070 | LMBP 12906 | MG1655(DE3) | glmS-pstS | none | PT7 | high | PelBss | sfgfp | tetA |
| SEGA071 | LMBP 12907 | MG1655(DE3) | glmS-pstS | none | PT7 | low | YebF | sfgfp | tetA |
| SEGA072 | LMBP 12908 | MG1655(DE3) | glmS-pstS | none | PT7 | medium | YebF | sfgfp | tetA |
| SEGA073 | LMBP 12909 | MG1655(DE3) | glmS-pstS | none | PT7 | high | YebF | sfgfp | tetA |
| SEGA074 | LMBP 12910 | MG1655 | glmS-pstS | none | PsaI-RiboJ | low | BCD | gfp | tetA |
| SEGA075 | LMBP 12911 | MG1655 | glmS-pstS | none | PsaI-RiboJ | high | BCD | gfp | tetA |
| SEGA076 | LMBP 12912 | MG1655 | glmS-pstS | none | PsaI-RiboJ | low | BCD | gfp | tetA |
| SEGA077 | LMBP 12913 | MG1655 | glmS-pstS | none | PsaI-RiboJ | high | BCD | gfp | tetA |
| SEGA078 | LMBP 12914 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | low | BCD | gfp | tetA |
| SEGA079 | LMBP 12915 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | medium | BCD | gfp | tetA |
| SEGA080 | LMBP 12916 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | high | BCD | gfp | tetA |
| SEGA081 | LMBP 12917 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | low | Ubiquitin | gfp | tetA |
| SEGA082 | LMBP 12918 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | medium | Ubiquitin | gfp | tetA |
| SEGA083 | LMBP 12919 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | high | Ubiquitin | gfp | tetA |
| SEGA084 | LMBP 12920 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | low | TEV | gfp | tetA |
| SEGA085 | LMBP 12921 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | medium | TEV | gfp | tetA |
| SEGA086 | LMBP 12922 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | high | TEV | gfp | tetA |
| SEGA087 | LMBP 12923 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | low | PROTi | gfp | tetA |
| SEGA088 | LMBP 12924 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | medium | PROTi | gfp | tetA |
| SEGA089 | LMBP 12925 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | high | PROTi | gfp | tetA |
| SEGA090 | LMBP 12926 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | low | PelBss | sfgfp | tetA |
| SEGA091 | LMBP 12927 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | medium | PelBss | sfgfp | tetA |
| SEGA092 | LMBP 12928 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | high | PelBss | sfgfp | tetA |
| SEGA093 | LMBP 12929 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | low | YebF | sfgfp | tetA |
| SEGA094 | LMBP 12930 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | medium | YebF | sfgfp | tetA |
| SEGA095 | LMBP 12931 | MG1655 ∆galK | glmS-pstS | galK | PrhaBAD | high | YebF | sfgfp | tetA |
| SEGA096 | LMBP 12932 | MG1655 | glmS-pstS | none | J23100 | low | BCD | none | 3'tetA |
| SEGA097 | LMBP 12933 | MG1655 | glmS-pstS | none | J23100 | high | BCD | none | 3'tetA |
| SEGA098 | LMBP 12934 | MG1655 | glmS-pstS | none | J23100 | high | BCD | gfp | 3'tetA |
| SEGA099 | LMBP 12935 | MG1655 | glmS-pstS | none | Ptrc | low | BCD | none | 3'tetA |
| SEGA100 | LMBP 12936 | MG1655 | glmS-pstS | none | Ptrc | high | BCD | none | 3'tetA |
| SEGA101 | LMBP 12937 | MG1655 | glmS-pstS | none | PrhaBAD | low | BCD | none | 3'tetA |
| SEGA102 | LMBP 12938 | MG1655 | glmS-pstS | none | PrhaBAD | high | BCD | none | 3'tetA |
| SEGA103 | LMBP 12939 | MG1655(DE3) | glmS-pstS | none | PT7 | low | BCD | none | 3'tetA |
| SEGA104 | LMBP 12940 | MG1655(DE3) | glmS-pstS | none | PT7 | high | BCD | none | 3'tetA |