Maldi-Tof MS technology has proven to be a fast and reliable method for the identification of bacteria and yeasts.
However, for the identification of filamentous fungi, this technology is not yet routinely used.
The reason is firstly, that no simple standardized procedure currently exists that permits reproducible results for
the whole range of fungal species and secondly, the fact that the database with reference spectra that is currently
commercially available, is rather limited.
BCCM/IHEM is currently constructing a Maldi-Tof MS reference database for clinically relevant filamentous fungi
in collaboration with 'Laboratoire de Parasitologie-Mycologie' of the CHU Timone in Marseille (France).
Reference strains of the BCCM/IHEM collection are used to build a reference database,
with a particular architecture allowing to face the problem of intra-species variability.
Next to that, clinical isolates are used, to improve the reliability of the constructed database.
The performance of the database will be validated using both clinical isolates and other reference strains
from the BCCM/IHEM collection.
Finally, the aim is to validate this database and this technology for the quality control of our strains according
to the ISO9001 standard, and to offer the rapid identification of filamentous fungi and yeasts as a service for our customers.