High quality genomic DNA at BCCM/LMG

In addition to the supply of bacteria in freeze-dried form, in frozen form, or as active cultures, BCCM/LMG also provides high quality genomic DNA from any publicly available strain on request. Genomic DNA is obtained using a protocol that includes at least two chloroform extraction steps. Quality control is performed spectrophotometrically and by agarose gel electrophoresis. A minimum amount of 2,5 µg of high molecular mass (average length over 10 kbp) genomic DNA with an A_260nm/_A280nm ratio of 1.7-2.2 is guaranteed to the customer. Larger amounts of 25 µg can also be ordered. The DNAs are stored in 0.1 x TE buffer (1 mM Tris pH 8, 0.1 mM EDTA pH 8) at -20°C and are generally sent at room temperature by courier.