This handbook is describing the molecular monitoring of toxigenicity of cyanobacteria (blue-green algae) in surface waters including lakes, rivers, drinking water reservoirs and food supplements. In water bodies, cyanobacteria mass developments and cyanobacteria harmful algal blooms (cHAB) are highly favoured by eutrophication and global temperature rise.
Thus, a number of introductory chapters covering the various subjects is followed by a series of Standard Operational Procedures (SOPs). The list of SOPs contains the molecular tests that could be routinely used in monitoring. The introductory chapters are concise papers written according to an a priori given outline, covering the purpose, methodological details and advantages of the various tools. Although through each introductory chapter the individual SOPs are referred to, each SOP should be considered as an independent unit with its own (essential) references section. All published SOPs have been used in the lab of the respective authors for years and are considered robust. They are based on reference strains which are reliably available from international culture collections.
The book is intended to be used by trained professionals analyzing toxigenicity of water samples in the laboratory in both academic and governmental institutions, as well as technical offices and agencies which are in charge of water body surveillance and monitoring. Students will learn important methods’ standards of essential protocols including steps from sampling until results evaluation. More generally the book will benefit the reader by (i) increasing the knowledge of the currently available molecular tool box, and (ii) enable the reader to carry out state-of-the-art analyses on the toxigenicity of cyanobacteria in surface water.