PROGRAM

DAY 1: September 14

'Managing a culture collection'

Full day seminar

Information management
Quality control
Shipment
Nagoya protocol
Introduction on the use of molecular tools (presentation GeneCorner)

DAY 2-4: September 15-17

3 days training on handling and preservation of an organism type of your choice

Bacteria up to biosafety level 2
	1. Handling of bacteria: GLP	Isolation and culturing Purity check and quality controls Working under ISO9001
	2. Freeze-drying	Concept and overview Residual Moisture Content determination Hands on training Data management
	3. Cryopreservation	Concept and overview Hands on training Data management
	4. Safeguarding strictly anaerobic cultures	Concept and overview
	5. Encapsulation dehydration vitrification	Concept and overview
	6. DNA bank	Concept and overview
	7. Viability	Concept and overview
	8. General discussion	Question and Answer session
(Myco)bacteria up to biosafety level 3
	1. Handling of Bacteria: GLP	Isolation and culturing Purity check and quality controls Working under ISO9001
	2. Freeze-drying	Concept and overview Residual Moisture Content determination Hands on training Data management
	3. Viability & purity checks	Methods for viability & purity assessment Data management
	4. Cryopreservation (liquid nitrogen)	Concept and overview Demonstration
	5. Cryopreservation (-80°C)	Concept and overview Hands-on training Data management
	6. DNA-bank	Concept and overview
	7. General discussion	Question and Answer session
Cyanobacteria
	1. Handling of Cyanobacteria: GLP	Working under ISO9001
	2. Microscopical observations and taxonomy	Hands-on training
	3. Culturing and culture media	Sterile transfers for preservation of living cultures Hands-on training
	4. Isolation from environmental samples and purification	Purity controls Hands-on training
	5. Cryopreservation (-80°C)	Concept and overview Hands-on training
	6. DNA bank	Concept and overview
	5. General discussion	Question and Answer session
Fungi
	1. Handling of Fungi: GLP	Isolation Culture media Culture
	2. Freeze-drying	Concept and overview Hands on training
	3. Cryopreservation	Concept and overview Hands on training
	4. Cryopreservation on cryobeads	Concept and overview Hands on training
	5. Preservation under water	Concept and overview Hands on training
	6. General discussion	Question and Answer session Future perspectives
Microalgae specialised in diatoms
	1. Handling of Microalgae: GLP	Isolation Culture media Culture
	2. Stock cultures	Concept and overview Hands on training
	3. Diatom life cycle	Concept and overview Hands on training
	4. Cryopreservation and thawing	Concept and overview Hands on training
	5. General discussion	Question and Answer session Future perspectives