Projects at consortium level
Projects on collection level
- BCCM/LMBP Plasmid Collection
- BCCM/LMG Bacteria Collection
- BCCM/IHEM Biomedical Fungi and Yeasts Collection
- BCCM/MUCL Agro-food & Environmental Fungal Collection
- BCCM/ULC Cyanobacteria Collection
TRUST - TRansparent User-friendly System of Transfer, implementing the Nagoya Protocol in microbiology
MOSAICS - Development of a system for appropriate management of access to and transfer of microbiological resources
Cloning and functional analysis of genes from bacterial defense systems. We focus on Salmonella typhimurium and Pseudomonas aeruginosa, for which several type II, III, IV and VI secretion system genes are already available in the public collection.
Construction of unique gene family plasmid libraries. Our current focus is on the creation of a tagged expression library for membrane targeting of different proteins. This expression library is derived from plasmids of the human ORFeome database described by Yang et al. (2011; PMID: 21706014), which is publicly available at BCCM/LMBP.
Optimisation and validation of long-term plasmid DNA storage methods.
Collaboration with the VIB Microscopy Core Facility (VIB, Ghent, Belgium) for the construction of plasmids expressing APEX-tagged proteins which are useful for electron microscopy. A Gateway entry vector containing the APEX gene for the creation of N-terminally tagged protein expression vectors is available in the public collection.
Arabidopsis Genomic RNAi Knock-out Line Analysis (AGRIKOLA). Collaboration with the Department of Plant Systems Biology (VIB, Ghent, Belgium) to validate a genome-scale library of Gateway plasmids for gene silencing in Arabidopsis thaliana. The entire library is publicly available via BCCM/LMBP.
Screening and genetic manipulation of fungi for improved protein secretion and N-glycosylation. Collaboration with BCCM/MUCL and the Center for Transgene Technology and Gene Therapy (KUL, Leuven, Belgium) for the analysis of the N-glycan profile of yeast and fungi and the conversion to more human-like structures. Yeast Surface Display was implemented in Pichia pastoris as a tool to increase secretion of proteins that are difficult to express.
- The gut-renal axis: link between plasma levels of intestinally generated uraemic toxins and gut microbiome profiles in consecutive stages of chronic kidney disease (FWO; 2015-2018)
Partners: Prof. G. Glorieux (UZ Gent, promoter), Prof. G. Huys (UGent & BCCM/LMG; co-promoter), Prof. M. Joossens (VIB-KU Leuven; co-promoter)
- Diversity and nutritional modulation of fecal microbiota in the captive cheetah (BOF-UGent; 2010-2015)
Partners: Prof. G. Janssens (UGent, promoter), Prof. G. Huys (UGent & BCCM/LMG; co-promoter), Prof. M. Hesta (UGent; co-promoter)
- Development of a software package for the prediction of microbial shelf life in packed cooked meat products: a tool for innovation and product development in the meat product sector (IWT-TETRA; 2013-2015)
Partners: Prof. F. Devlieghere (UGent, promoter), Prof. G. Huys (UGent & BCCM/LMG; co-promoter), Prof. J. Van Impe (KU Leuven; co-promoter)
- A 12 years trend analysis of candidaemia in Belgium: genotyping, drug resistance and antifungal consumption (WIV-ISP grant, 2014-2017)
Partners: Prof. K. Lagrou (KUL, academic promoter), Dr. B. Catry (WIV-ISP)
- Identification of filamentous fungi by MALDI-TOF mass spectrometry: reference database, online identification and epidemiology
Partners: Prof. R. Piarroux (University of Marseille)
Antibiotics and anti-quorum sensing compounds from African fungal endophytes inhabiting medicinal plants and cultures of macromycetes – ERANET (2014-2018).
BENEFECT - Valorization of beneficial soil microorganisms as agro-ecological alternative to mineral fertilizers in maize production systems – Operational Directorate for Agriculture, Natural Resources and Environment (01/02/2017 - 31/01/2019).
CUDROP - Blight control in potato by the use of agents/microbial compounds and implementation of innovative application processes – Operational Directorate for Agriculture, Natural Resources and Environment (2016-31/01/2018).
Reinforcement of the fungal expertise in Ecuador via case studies of fungal plants interactions in selected ecosystems and the development of biotechnology-oriented fungal resource centres – CIUF (2011-2016)
- Collaboration with Prof. Emmanuelle Javaux (Geology Dept, Ulg) for the FRFC project « Fossilisation des cyanobactéries : approches phylogénétique, micropaléontologique et sédimentologique et implications pour l’évolution de la biosphère primitive » ; 2009-12.
- Masters thesis. Yasmine Ould Amer ‘ Etude du potentiel de synthèse de composés secondaires bioactifs par des Cyanobactéries’ (Master in Biochemistry, Molecular and Cellular Biology, U. Liège, Belgium) ; June 2012.
- Bilateral cooperation project WBI/Poland on the Application of ‘Next Generation Sequencing’ (NGS) approaches for the design of genetic engineering tools for Arthrospira strains (cyanobacterium).; 2013-14
- Bilateral cooperation (FNRS-CNPq) with Prof. Fiore Marli (CENA, Piracicaba, Brazil) to study the production of bioactive compounds of Antarctic strains; 2012.
- We have a bilateral cooperation project with Prof. Albertano and Dr. Billi (University Tor Vergata, Rome, Italy) concerning the phylogeny of Chroococidiopsis, a cyanobacterial genus found in cold and hot deserts
- Since November 2013, Dr Julia Kleinteich has obtained a 2-year BeIPD‐COFUND‐IPD fellowship to work on the production of cyanotoxins and secondary metabolites by BCCM/ULC strains.