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BCCM/GeneCorner Plasmid Collection

 

The BCCM/GeneCorner Plasmid Collection accepts plasmids from and distributes plasmids to researchers worldwide. Funding by the Belgian Science Policy (Belspo) allowed BCCM/GeneCorner to evolve into a unique plasmid repository in Europe.

Embedding

BCCM/GeneCorner is embedded in the Department of Biomedical Molecular Biology (DBMB, Ghent University) and the VIB-UGent Center for Inflammation Research (IRC, VIB), which together form the host laboratory for BCCM/GeneCorner. 

The BCCM/GeneCorner activities are continuously supported by the research activities of its host laboratory, which focus on molecular and cellular mechanisms that are at the origin of chronic inflammation, autoimmune diseases, infectious diseases and cancer. These mechanisms are mainly studied in cellular and animal model systems by using the most advanced methods of molecular biology, cell biology and immunology. Expression plasmids for human and mouse genes are frequently generated and extensively used, offering an ideal environment for BCCM/GeneCorner. Research of the host laboratory is multidisciplinary with molecular biologists, immunologists, cell biologists, and bioinformaticians collaborating with clinicians. It is the ambition of the host laboratory to translate basic research into innovative ways for diagnosis and therapy of human diseases.

Click here for a list of publications of the host laboratory.

BCCM/GeneCorner has already performed a number of research projects to improve plasmid DNA storage and to enlarge its scope of basic vectors and genes in view of the needs of the academic and industrial communities, and is continuously interested in project-based scientific collaborations on these issues.

 

Scope of the biological material

The BCCM/GeneCorner Plasmid Collection warrants the long-term storage and distribution of plasmids, microbial host strains and DNA libraries of fundamental, biotechnological, educational or general scientific importance.

The focus is on the collection of recombinant plasmids that can replicate in a microbial host strain, with main interest for:

  • empty cloning plasmids useful for recombinant (over)expression of proteins of interest in bacteria, yeast, fungi, insect cells, plant and animal cells;
  • (expression) plasmids carrying specific protein-coding genes and derivatives from a wide range of organisms (human, mouse, various bacterial species, ...);
  • expression plasmids with specific non-coding genes such as shRNA and miRNA;
  • plasmids for mouse genetic engineering, allowing the generation of transgenic mice.

BCCM/GeneCorner also accepts natural and genetically modified animal or human cell lines, including hybridomas, as well as other genetic material, in the safe deposit and patent deposit collections.

 

Quality

BCCM/GeneCorner is ISO 9001:2015 certified for accession, control, preservation, storage and supply of biological material and related information in the frame of public deposits, safe deposits and patent deposits.

 

Quality control by Next-Generation Sequencing @ GeneCorner

BCCM/GeneCorner has implemented Next-Generation Sequencing as a default quality control for the authenticity of its publicly available plasmids. Since 2019, plasmids deposited in the public collection will be analysed by NGS. This way, BCCM/GeneCorner can deliver even higher quality plasmids!

In times past, authenticity checks on GeneCorner’s plasmids were performed via restriction enzyme pattern analysis, which was carried out upon receipt of an order. Whenever this analysis was inconclusive, or showed discrepancies with the sequence obtained from the depositor, a more in-depth check was performed via Sanger sequencing.

At present, plasmids that are newly deposited in the public collection, are checked for their authenticity via Next-Generation Sequencing. In case of plasmid libraries (same backbone vector, different cloned genes), a number of plasmids is selected for NGS. The bulk of the library is checked by Sanger sequencing of the insert.

All sequence information obtained by GeneCorner is made publically accessible through our plasmid catalogue.

Plasmids that have not been sequenced (yet) only show the sequence provided by the depositor. Sanger and NGS results are made available as fasta files. Once all plasmid data have been processed to the full data set, the plasmid sequence, as modified and annotated by GeneCorner, is available for download, both in Genbank (.gb) and EMBL (.txt) format, along with a visual plasmid map (.pdf).

 

A project by Uchrony