LOCUS       pGB-dummy 07;08         2709 bp ds-DNA     circular SYN 29-JUL-2016
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2709)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Thursday, Nov 28, 2019 from SnapGene Viewer 5.0.6 to Vector
            NTI(R) format
            https://www.snapgene.com
COMMENT     VNTDATE|776649600|
COMMENT     VNTDBDATE|776649600|
COMMENT     VNTNAME|pGB-dummy 07;08|
FEATURES             Location/Qualifiers
     rep_origin      1..683
                     /label=ColE1 origin
     misc_binding    1023..1045
                     /label=LacO
     primer_bind     1053..1074
                     /label=SP6
     misc_feature    1086..1091
                     /label=BsaI site
     misc_feature    1093..1096
                     /label=overhang 07
     misc_feature    1097..1119
                     /label=dummy
     misc_feature    1120..1123
                     /label=overhang 08
     misc_feature    complement(1125..1130)
                     /label=BsaI site(1)
                     /label=BsaI site
     primer_bind     complement(1140..1160)
                     /label=T7
     CDS             1248..1316
                     /label=LacZ alpha
     CDS             1755..2615
                     /label=Beta lactamase
ORIGIN
        1 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
       61 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
      121 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
      181 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
      241 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
      301 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
      361 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
      421 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
      481 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
      541 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
      601 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
      661 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
      721 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
      781 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
      841 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
      901 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
      961 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     1021 gtggaattgt gagcggataa caatttcaca cgagctattt aggtgacact atagaagtga
     1081 agcttggtct cacctgggct gagtaggcaa gatgttctgc ggatgagacc gaattctcgc
     1141 cctatagtga gtcgtattac ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     1201 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     1261 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc
     1321 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
     1381 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     1441 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     1501 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     1561 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     1621 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     1681 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     1741 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     1801 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     1861 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     1921 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     1981 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     2041 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     2101 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     2161 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     2221 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     2281 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     2341 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     2401 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     2461 gtgagcgtgg ctctcgaggt atcattgcag cactggggcc agatggtaag ccctcccgta
     2521 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     2581 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     2641 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     2701 ttgataatc
//
