LOCUS pGB-03;04_ccdB 3470 bp ds-DNA circular SYN 10-DEC-2018 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 3470) AUTHORS . TITLE Direct Submission JOURNAL Exported Thursday, Nov 28, 2019 from SnapGene Viewer 5.0.6 to Vector NTI(R) format https://www.snapgene.com COMMENT VNTDATE|860716800| COMMENT VNTDBDATE|860716800| COMMENT VNTNAME|pGB-03;04_ccdB| FEATURES Location/Qualifiers misc_feature 1..224 /label=Tn7R misc_feature 291..824 /label=GmR misc_recomb 1112..1145 /label=LoxP misc_feature 1149..1175 /label=I-CeuI misc_feature 1176..1181 /label=BsaI site misc_feature 1183..1186 /label=overhang 03 misc_feature 1234..1363 /label=polyhedrin promoter misc_feature 1326..1328 /label=2nd start misc_feature 1361 /label=Insertion to outframe 2nd start misc_feature 1370..1380 /label=kozak misc_feature 1390..1479 /label=TwinStrep misc_feature 1489..1512 /label=Prescission 3C misc_feature 1638..1946 /label=ccdB misc_feature 1916..1939 /label=vec_ccdB_F misc_feature 1966..1983 /label=HIS6 polyA_signal complement(2066..2257) /label=SV40 late polyA misc_feature 2382..2385 /label=overhang 04 misc_feature complement(2387..2392) /label=BsaI site(1) /label=BsaI site misc_feature 2393..2558 /label=Tn7L rep_origin 2672..3354 /label=ColE1 origin ORIGIN 1 tgtgggcgga caataaagtc ttaaactgaa caaaatagat ctaaactatg acaataaagt 61 cttaaactag acagaatagt tgtaaactga aatcagtcca gttatgctgt gaaaaagcat 121 actggacttt tgttatggct aaagcaaact cttcattttc tgaagtgcaa attgcccgtc 181 gtattaaaga ggggcgtggc caagggcatg taaagactat attcgcggcg ttgtgacaat 241 ttaccgaaca actccgcggc cgggaagccg atctcggctt gaacgaattg ttaggtggcg 301 gtacttgggt cgatatcaaa gtgcatcact tcttcccgta tgcccaactt tgtatagaga 361 gccactgcgg gatcgtcacc gtaatctgct tgcacgtaga tcacataagc accaagcgcg 421 ttggcctcat gcttgaggag attgatgagc gcggtggcaa tgccctgcct ccggtgctcg 481 ccggagactg cgagatcata gatatagatc tcactacgcg gctgctcaaa cttgggcaga 541 acgtaagccg cgagagcgcc aacaaccgct tcttggtcga aggcagcaag cgcgatgaat 601 gtcttactac ggagcaagtt cccgaggtaa tcggagtccg gctgatgttg ggagtaggtg 661 gctacgtctc cgaactcacg accgaaaaga tcaagagcag cccgcatgga tttgacttgg 721 tcagggccga gcctacatgt gcgaatgatg cccatacttg agccacctaa ctttgtttta 781 gggcgactgc cctgctgcgt aacatcgttg ctgctgcgta acatcgttgc tgctccataa 841 catcaaacat cgacccacgg cgtaacgcgc ttgctgcttg gatgcccgag gcatagactg 901 tacaaaaaaa cagtcataac aagccatgaa aaccgccact gcgccgttac caccgctgcg 961 ttcggtcaag gttctggacc agttgcgtga gcgcatacgc tacttgcatt acagtttacg 1021 aaccgaacag gcttatgtca actgggttcg tgccttcatc cgtttccacg gtgtgcgtca 1081 cccggcaacc ttgggcagca gcgaagtcgc cataacttcg tatagcatac attatacgaa 1141 gttatctgta actataacgg tcctaaggta gcgaaggtct caggctgttt aaacactagt 1201 atcgattcgc gacctactcc ggaatattaa tagatcatgg agataattaa aatgataacc 1261 atctcgcaaa taaataagta ttttactgtt ttcgtaacag ttttgtaata aaaaaaccta 1321 taaatattcc ggattattca taccgtccca ccatcgggcg acggatcccg gtccgaaacc 1381 atgaaaggga gcgcttggag ccacccgcag ttcgaaaaag gtggaggttc tggcggtgga 1441 tcgggaggtt cagcgtggag ccacccgcag ttcgagaaaa ccgcgggtct ggaagttctg 1501 ttccaggggc ccatggacta atgaggtacc ggatccgaat tcgagctccg tcgacaagct 1561 tgatatcgaa ttcctgcagc ccaaactgca gttgacaaca taaaaacttt gtgttatact 1621 tgtaacgtaa ggaggtaatg attcagttta aggtttacac ctataaaaga gagagccgtt 1681 atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga 1741 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg 1801 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct 1861 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca 1921 ttaacctgat gttctgggga atataagctt gcggccgcac tcgagcacca ccaccaccac 1981 cactgacatg cggtaccaag cttgtcgaga agtactagag gatcataatc agccatacca 2041 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac 2101 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat 2161 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg 2221 gtttgtccaa actcatcaat gtatcttatc atgtctggat ctgatcactg cttgagccta 2281 gaagatccgg ctgctaacaa agcccgaaag gaagctgagt tggctgctgc caccgctgag 2341 caataactat cataacccct agggtatacc catctaattg gtcagtgaga ccaaccagat 2401 aagtgaaatc tagttccaaa ctattttgtc atttttaatt ttcgtattag cttacgacgc 2461 tacacccagt tcccatctat tttgtcactc ttccctaaat aatccttaaa aactccattt 2521 ccacccctcc cagttcccaa ctattttgtc cgcccacaac cggttgactt gggtcaactg 2581 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa 2641 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt 2701 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt 2761 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt 2821 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag 2881 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta 2941 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat 3001 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg 3061 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg 3121 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac 3181 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga 3241 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt 3301 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta 3361 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat 3421 tgacttgggt cgctcttcct gtggatgcgc agatgccctg cgtaagcggg //