LOCUS       pLenti6-hRIPK4-K51R-HA-puro        9520 bp    DNA     circular     17-OCT-2016
DEFINITION  LMBP 10155.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10155
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            
            
COMMENT     VNTDATE|-11462000|
COMMENT     VNTNAME|pLenti6-hRIPK4-K51R-HA-puro|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT            (CStringList) (CStringList) 1 1 1 1 "") 
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COMMENT        #4=(CFSignal (CObList) "RSV/HIV-1 5' LTR" 29 0 0 4766 5094 0 
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COMMENT                             #216=(CLabel (CWidget 0 (0 0) 1 2 0 0 #170# 0 100) 
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COMMENT                                   #222#) 
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COMMENT                                   #224#) 
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FEATURES             Location/Qualifiers
     promoter        120..402
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             511..1113
                     /vntifkey="4"
                     /label=puromycin\resistance
     rep_origin      359..434
                     /vntifkey="33"
                     /label=SV40\ori
     terminator      1510..1584
                     /vntifkey="43"
                     /label=SV40\polyA\early
     promoter        4766..5094
                     /vntifkey="29"
                     /label=RSV/HIV-1\5'\LTR
     rep_origin      2013..2468
                     /vntifkey="33"
                     /label=f1\origin
     promoter        6491..7007
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     CDS             2599..3459
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      4000..4402
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        4494..4577
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        4641..4657
                     /vntifkey="30"
                     /label=T3\promoter
     terminator      complement(1510..1584)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      1720..1795
                     /vntifkey="33"
                     /label=SV40\ori
     promoter        complement(1829..1845)
                     /vntifkey="30"
                     /label=T7\promoter
     misc_feature    5205..5249
                     /vntifkey="21"
                     /label=HIV-1\psi\packaging\signal
     misc_feature    5759..5992
                     /vntifkey="21"
                     /label=HIV-1\Rev\response\element
     terminator      1204..1438
                     /vntifkey="43"
                     /label=RSV/HIV-1\dU3/3'\LTR
     misc_feature    1188..1203
                     /vntifkey="21"
                     /label=HIV-1\cPPT
     attenuator      7124..7148
                     /vntifkey="0"
                     /label=attB1
     attenuator      complement(9510..14)
                     /vntifkey="0"
                     /label=attB2
     misc_marker     58..84
                     /vntifkey="22"
                     /label=HA\epitope
     CDS             7154..9505
                     /vntifkey="4"
                     /label=hRIPK4\mutant
     mutation        7304..7306
                     /vntifkey="62"
                     /label=K51R
                     /note="WT: AAG"
BASE COUNT     2300 a      2520 c      2606 g      2094 t 
ORIGIN
        1 ttgtacaaag tggttgatat ccagcacagt ggcggccgct cgagtctaga gggcccgtac 
       61 ccctacgacg tgcccgacta cgccacgcgt accggttagt aatgagtttg gaattaattc 
      121 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccaggc aggcagaagt 
      181 atgcaaagca tgcatctcaa ttagtcagca accaggtgtg gaaagtcccc aggctcccca 
      241 gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccatagt cccgccccta 
      301 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga 
      361 ctaatttttt ttatttatgc agaggccgag gccgcctctg cctctgagct attccagaag 
      421 tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa agctcccggg agcttgtata 
      481 tccattttcg gatctgatca gcacgtgacc atggccaccg agtacaagcc cacggtgcgc 
      541 ctcgccaccc gcgacgacgt cccccgggcc gtacgcaccc tcgccgccgc gttcgccgac 
      601 taccccgcca cgcgccacac cgtcgacccg gaccgccaca tcgagcgggt caccgagctg 
      661 caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt cgcggacgac 
      721 ggcgccgcgg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc 
      781 gagatcggct cgcgcatggc cgagttgagc ggttcccggc tggccgcgca gcaacagatg 
      841 gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc caccgtcggc 
      901 gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc cggagtggag 
      961 gcggccgagc gcgctggggt gcccgccttc ctggagacct ccgcgccccg caacctcccc 
     1021 ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc 
     1081 acctggtgca tgacccgcaa gcccggtgcc tgagctaagc acaattcgag ctcggtacct 
     1141 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg 
     1201 ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg 
     1261 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg 
     1321 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt 
     1381 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt 
     1441 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag 
     1501 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca 
     1561 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc 
     1621 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc 
     1681 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg 
     1741 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg 
     1801 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc 
     1861 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc 
     1921 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc 
     1981 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc 
     2041 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc 
     2101 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct 
     2161 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa 
     2221 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc 
     2281 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact 
     2341 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg 
     2401 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct 
     2461 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc 
     2521 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa 
     2581 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt 
     2641 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct 
     2701 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc 
     2761 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta 
     2821 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac 
     2881 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc 
     2941 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac 
     3001 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg 
     3061 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac 
     3121 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc 
     3181 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt 
     3241 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga 
     3301 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc 
     3361 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag 
     3421 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca 
     3481 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc 
     3541 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca 
     3601 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc 
     3661 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta 
     3721 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt 
     3781 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc 
     3841 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg 
     3901 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg 
     3961 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag 
     4021 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc 
     4081 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat 
     4141 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg 
     4201 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc 
     4261 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt 
     4321 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca 
     4381 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg 
     4441 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac 
     4501 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg 
     4561 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac 
     4621 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa 
     4681 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc 
     4741 aacatgcctt acaaggagag aaaaagcacc gtgcatgccg attggtggaa gtaaggtggt 
     4801 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg 
     4861 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc 
     4921 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta 
     4981 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact 
     5041 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg 
     5101 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg 
     5161 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat 
     5221 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg 
     5281 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa 
     5341 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct 
     5401 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac 
     5461 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca 
     5521 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa 
     5581 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga 
     5641 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag 
     5701 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag 
     5761 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga 
     5821 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc 
     5881 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc 
     5941 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt 
     6001 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta 
     6061 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta 
     6121 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga 
     6181 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa 
     6241 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa 
     6301 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat 
     6361 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag 
     6421 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc 
     6481 gataagcttg ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc 
     6541 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag 
     6601 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac 
     6661 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg 
     6721 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg 
     6781 tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat gggcgtggat 
     6841 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt 
     6901 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc ccattgacgc 
     6961 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc 
     7021 gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga caccgactct 
     7081 agaggatcca ctagtccagt gtggtggaat tctgcagata tcaacaagtt tgtacaaaaa 
     7141 agcaggctcc accatggagg gcgacggcgg gaccccatgg gccctggcgc tgctgcgcac 
     7201 cttcgacgcg ggcgagttca cgggctggga gaaggtgggc tcgggcggct tcgggcaggt 
     7261 gtacaaggta cgccatgtcc actggaagac ctggctggcc atcaggtgct cgcccagcct 
     7321 gcacgtcgac gacagggagc gcatggagct tttggaagaa gccaagaaga tggagatggc 
     7381 caagtttcgc tacatcctgc ctgtgtatgg catctgccgc gaacctgtcg gcctggtcat 
     7441 ggagtacatg gagacgggct ccctggaaaa gctgctggct tcggagccat tgccatggga 
     7501 tctccggttc cgaatcatcc acgagacggc ggtgggcatg aacttcctgc actgcatggc 
     7561 cccgccactc ctgcacctgg acctcaagcc cgcgaacatc ctgctggatg cccactacca 
     7621 cgtcaagatt tctgattttg gtctggccaa gtgcaacggg ctgtcccact cgcatgacct 
     7681 cagcatggat ggcctgtttg gcacaatcgc ctacctccct ccagagcgca tcagggagaa 
     7741 gagccggctc ttcgacacca agcacgatgt atacagcttt gcgatcgtca tctggggcgt 
     7801 gctcacacag aagaagccgt ttgcagatga gaagaacatc ctgcacatca tggtgaaggt 
     7861 ggtgaagggc caccgccccg agctgccgcc cgtgtgcaga gcccggccgc gcgcctgcag 
     7921 ccacctgata cgcctcatgc agcggtgctg gcagggggat ccgcgagtta ggcccacctt 
     7981 ccaagaaatt acttctgaaa ccgaggacct gtgtgaaaag cctgatgacg aagtgaaaga 
     8041 aactgctcat gatctggacg tgaaaagccc cccggagccc aggagcgagg tggtgcctgc 
     8101 gaggctcaag cgggcctctg cccccacctt cgataacgac tacagcctct ccgagctgct 
     8161 ctcacagctg gactctggag tttcccaggc tgtcgagggc cccgaggagc tcagccgcag 
     8221 ctcctctgag tccaagctgc catcgtccgg cagtgggaag aggctctcgg gggtgtcctc 
     8281 ggtggactcc gccttctctt ccagaggatc actgtcgctg tcctttgagc gggaaccttc 
     8341 aaccagcgat ctgggcacca cagacgtcca gaagaagaag cttgtggatg ccatcgtgtc 
     8401 cggggacacc agcaaactga tgaagatcct gcagccgcag gacgtggacc tggcactgga 
     8461 cagcggtgcc agcctgctgc acctggcggt ggaggccggg caagaggagt gcgccaagtg 
     8521 gctgctgctc aacaatgcca accccaacct gagcaaccgt aggggctcca ccccgttgca 
     8581 catggccgtg gagaggaggg tgcggggtgt cgtggagctc ctgctggcgc ggaagatcag 
     8641 tgtcaacgcc aaggatgagg accagtggac agccctccac tttgcagccc agaacgggga 
     8701 cgagtctagc acacggctgc tgttggagaa gaacgcctcg gtcaacgagg tggactttga 
     8761 gggccggacg cccatgcacg tggcctgcca gcacgggcag gagaatatcg tgcgcatcct 
     8821 gctgcgccga ggcgtggacg tgagcctgca gggcaaggat gcctggctgc cactgcacta 
     8881 cgctgcctgg cagggccacc tgcccatcgt caagctgctg gccaagcagc cgggggtgag 
     8941 tgtgaacgcc cagacgctgg atgggaggac gccattgcac ctggccgcac agcgcgggca 
     9001 ctaccgcgtg gcccgcatcc tcatcgacct gtgctccgac gtcaacgtct gcagcctgct 
     9061 ggcacagaca cccctgcacg tggccgcgga gacggggcac acgagcactg ccaggctgct 
     9121 cctgcatcgg ggcgctggca aggaggccgt gacctcagac ggctacaccg ctctgcacct 
     9181 ggctgcccgc aacggacacc tggccactgt caagctgctt gtcgaggaga aggccgatgt 
     9241 gctggcccgg ggacccctga accagacggc gctgcacctg gctgccgccc acgggcactc 
     9301 ggaggtggtg gaggagttgg tcagcgccga tgtcattgac ctgttcgacg agcaggggct 
     9361 cagcgcgctg cacctggccg cccagggccg gcacgcacag acggtggaga ctctgctcag 
     9421 gcatggggcc cacatcaacc tgcagagcct caagttccag ggcggccatg gccccgccgc 
     9481 cacactcctg cggcgaagca agaccttgga cccagctttc 
//
