LOCUS       pCAGGS-Fpre             6415 bp    DNA     circular     17-AUG-2018
DEFINITION  LMBP 10251.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10251
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12425068|
COMMENT     VNTDBDATE|-12425068|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCAGGS-Fpre|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark-Zwijnaarde 71|
COMMENT     VNTOAUTHORAD3|B-9052 Gent Belgium|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        (CObList) -1 "") 
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COMMENT            0 1705 1796 0 (CStringList) (CStringList) 1 1 1 1 "91..182") 
COMMENT        #1=(CFSignal (CObList) "3' flanking region of rabbit \337-globin" 21 0 0 
COMMENT            1797 2153 0 (CStringList) (CStringList) 1 1 1 1 "183..539") 
COMMENT        #2=(CFSignal (CObList) "lac promoter" 30 0 1 2231 2313 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(617..699)") 
COMMENT        #3=(CFSignal (CObList) "SV40 ori" 33 0 0 2455 2530 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "841..916") 
COMMENT        #4=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2561 2635 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "947..1021") 
COMMENT        #5=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2561 2635 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(947..1021)") 
COMMENT        #6=(CFSignal (CObList) "pMB1 ori" 33 0 1 2750 3151 0 (CStringList) 
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COMMENT        #7=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3692 4552 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(2078..2938)") 
COMMENT        #8=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4688 5065 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "3074..3451") 
COMMENT        #9=(CFSignal (CObList) "AG promoter" 29 0 0 5071 6412 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "3457..4798") 
COMMENT        #10=(CFSignal (CObList) "intron AG promoter" 15 0 0 5440 6364 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "3826..4750") 
COMMENT        #11=(CFSignal (CObList) "Fpre" 4 0 0 18 1703 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pCAGGS-Fpre'" 1) 
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COMMENT                          "LMBP 10251." 1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
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COMMENT                          "Created: 08/17/18 11:43" 1) 
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COMMENT                          "Last Modified: 08/17/18 11:43" 1) 
COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6415 bp" 1) 
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COMMENT                          "Modification Date in the Original DB: 30-SEP-2013" 1))) 
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COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.genecorner@UGent.be</a>" 
COMMENT                          1) 
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COMMENT                          "WWW: <a href=\"http://www.genecorner.ugent.be\">www.genecorner.ugent.be</a>" 
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COMMENT                                   "intron AG promoter" "@N" 1 -10.568 10.454 0 
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COMMENT                                   "hCMV-IE enhancer" "@N" 1 -16.1225 0 0 -10 
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COMMENT                                   "3' UTR of rabbit \337-globin, incl. polyA" 
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FEATURES             Location/Qualifiers
     terminator      1705..1796
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_feature    1797..2153
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     promoter        complement(2231..2313)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     rep_origin      2455..2530
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     terminator      2561..2635
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(2561..2635)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     rep_origin      complement(2750..3151)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(3692..4552)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        4688..5065
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="human CMV-IE enhancer"
     promoter        5071..6412
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     intron          5440..6364
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid intron"
     CDS             18..1703
                     /vntifkey="4"
                     /label=Fpre
BASE COUNT     1427 a      1778 c      1761 g      1449 t 
ORIGIN
        1 ttcgcggccg cgccaccatg gaactgctga tcctgaaggc caacgccatc accaccatcc 
       61 tgaccgccgt gaccttctgc tttgccagcg gccagaacat caccgaggaa ttctaccaga 
      121 gcacctgtag cgccgtgtcc aagggctacc tgagcgccct gagaaccggc tggtacacca 
      181 gcgtgatcac catcgagctg agcaacatca aagaaaacaa gtgcaacggc accgacgcca 
      241 aagtgaagct gatcaagcag gaactggaca agtacaagaa tgccgtgacc gaactgcagc 
      301 tgctgatgca gagcaccccc gccaccaaca accgggccag aagagaactg cccagattca 
      361 tgaactacac cctgaacaac gccaaaaaga ccaacgtgac cctgagcaag aagcggaagc 
      421 ggcggttcct gggctttctg ctgggagtgg gaagcgccat tgctagcgga gtggccgtgt 
      481 gcaaggtgct gcacctggaa ggcgaagtga acaagatcaa gtccgccctg ctgagcacca 
      541 acaaggccgt ggtgtctctg agcaacggcg tgtccgtgct gacctttaag gtgctggatc 
      601 tgaagaacta catcgacaaa cagctgctgc ccatcctgaa caagcagagc tgcagcatca 
      661 gcaacatcga gacagtgatc gagttccagc agaagaacaa ccggctgctg gaaatcaccc 
      721 gcgagttcag cgtgaacgct ggcgtgacca cccccgtgtc cacctacatg ctgaccaaca 
      781 gcgagctgct gtccctgatc aacgacatgc ccatcaccaa cgaccagaaa aagctgatga 
      841 gcaacaacgt gcagatcgtg cggcagcaga gctactccat catgtgcatt atcaaagaag 
      901 aggtgctggc ctacgtggtg cagctgcctc tgtacggcgt gatcgacacc ccctgctgga 
      961 agctgcacac cagccctctg tgcaccacca acaccaaaga gggctccaac atctgcctga 
     1021 cccggaccga cagaggctgg tactgcgata atgccggctc cgtctcattc tttccacaag 
     1081 ccgagacatg caaagtgcag agcaaccggg tgttctgcga caccatgaac agcctgaccc 
     1141 tgccctccga agtgaatctg tgcaacgtgg acatcttcaa ccctaagtac gactgcaaga 
     1201 tcatgaccag caagaccgac gtgtccagct ccgtgatcac aagcctgggc gccatcgtgt 
     1261 cctgctacgg caagaccaag tgcaccgcca gcaacaagaa ccggggcatc atcaagacct 
     1321 tcagcaacgg ctgcgactac gtgtccaaca agggggtgga caccgtgtct gtgggcaaca 
     1381 ccctgtacta cgtgaacaaa caggaaggca agagcctgta cgtgaagggc gagcccatca 
     1441 tcaacttcta cgaccccctg gtgttcccca gcgacgagtt cgatgccagc atctcccaag 
     1501 tgaacgagaa gatcaaccag agcctggcct tcatcagaaa gtccgatgag ctgctgggcg 
     1561 gctacatccc tgaggcccct agagatggcc aggcctatgt gcggaaggac ggcgaatggg 
     1621 tgctgctgtc taccttcctg ctggtgccca ggggctccca ccatcatcat caccactggt 
     1681 cccaccccca gttcgagaag tgaagatctt tttccctctg ccaaaaatta tggggacatc 
     1741 atgaagcccc ttgagcatct gacttctggc taataaagga aatttatttt cattgcaata 
     1801 gtgtgttgga attttttgtg tctctcactc ggaaggacat atgggagggc aaatcattta 
     1861 aaacatcaga atgagtattt ggtttagagt ttggcaacat atgcccatat gctggctgcc 
     1921 atgaacaaag gttggctata aagaggtcat cagtatatga aacagccccc tgctgtccat 
     1981 tccttattcc atagaaaagc cttgacttga ggttagattt tttttatatt ttgttttgtg 
     2041 ttattttttt ctttaacatc cctaaaattt tccttacatg ttttactagc cagatttttc 
     2101 ctcctctcct gactactccc agtcatagct gtccctcttc tcttatggag atccctcgac 
     2161 ctgcagccca agcttggcgt aatcatggtc atagctgttt cctgtgtgaa attgttatcc 
     2221 gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct gggtgcctaa 
     2281 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac 
     2341 ctgtcgtgcc agcggatccg catctcaatt agtcagcaac catagtcccg cccctaactc 
     2401 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa 
     2461 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt 
     2521 gaggaggctt ttttggaggc ctaggctttt gcaaaaagct aacttgttta ttgcagctta 
     2581 taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact 
     2641 gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggatccgctg 
     2701 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 
     2761 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 
     2821 tcaaaggcgg taatacggtt atccacagaa tcagggataa cgcaggaaag aacatgtgag 
     2881 caaaaggcca gcaaaaggcc aggaaccgta aaaaggccgc gttgctggcg tttttccata 
     2941 ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg tggcgaaacc 
     3001 cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg cgctctcctg 
     3061 ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga agcgtggcgc 
     3121 tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc tccaagctgg 
     3181 gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt aactatcgtc 
     3241 ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact ggtaacagga 
     3301 ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg cctaactacg 
     3361 gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt accttcggaa 
     3421 aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt ggtttttttg 
     3481 tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct ttgatctttt 
     3541 ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg gtcatgagat 
     3601 tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt aaatcaatct 
     3661 aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt gaggcaccta 
     3721 tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc gtgtagataa 
     3781 ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg cgagacccac 
     3841 gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc gagcgcagaa 
     3901 gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg gaagctagag 
     3961 taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca ggcatcgtgg 
     4021 tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga tcaaggcgag 
     4081 ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct ccgatcgttg 
     4141 tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg cataattctc 
     4201 ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca accaagtcat 
     4261 tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata cgggataata 
     4321 ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct tcggggcgaa 
     4381 aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact cgtgcaccca 
     4441 actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa acaggaaggc 
     4501 aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc atactcttcc 
     4561 tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg 
     4621 aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac 
     4681 ctgggtcgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt 
     4741 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga 
     4801 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca 
     4861 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca 
     4921 gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg 
     4981 cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc 
     5041 tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg cttcactctc 
     5101 cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta attattttgt 
     5161 gcagcgatgg gggcgggggg gggggggggg ccccccccag gcggggcggg gcggggcgag 
     5221 gggcggggcg gggcgaggcg gaaaggtgcg gcggcagcca atcagagcgg cgcgctccga 
     5281 aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg aagcgcgcgg 
     5341 cgggcgggag tcgttgcgcg ctgccttccc cccgtgcccc gctccgccgc cgcctcgcgc 
     5401 cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg ggacggccct 
     5461 tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt tctgtggctg 
     5521 cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg ctcggggggt 
     5581 gcgtgcgtgt gtgtgtgcgt ggggagcgcc gcgtgcggct ccgcgctgcc cggcggctgt 
     5641 gagcgctgcg ggcgcggcgc ggggctttgt gcgctccgca gtgtgcgcga ggggagcgcg 
     5701 gccgggggcg gtgccccgcg gtgcgggggg ggctgcgagg ggaacaaagg ctgcgtgcgg 
     5761 ggtgtgtgcg tgggggggtg agcagggggt gtgggcgcgt cggtcgggct gcaacccccc 
     5821 ctgcaccccc ctccccgagt tgctgagcac ggcccggctt cgggtgcggg gctccgtacg 
     5881 gggcgtggcg cggggctcgc cgtgccgggc ggggggtggc ggcaggtggg ggtgccgggc 
     5941 ggggcggggc cgcctcgggc cggggagggc tcgggggaag gggcgcggcg gcccccggag 
     6001 cgccggcggc tgtcgaggcg cggcgagccg cagccattgc cttttatggt aatcgtgcga 
     6061 gagggcgcag ggacttcctt tgtcccaaat ctgtgcggag ccgaaatctg ggaggcgccg 
     6121 ccgcaccccc tctagcgggc gcggggcgaa gcggtgcggc gccggcagga aggaaatggg 
     6181 cggggagggc cttcgtgcgt cgccgcgccg ccgtcccctt ctccctctcc agcctcgggg 
     6241 ctgtccgcgg ggggacggct gccttcgggg gggacggggc agggcggggt tcggcttctg 
     6301 gcgtgtgacc ggcggctcta gagcctctgc taaccatgtt catgccttct tctttttcct 
     6361 acagctcctg ggcaacgtgc tggttattgt gctgtctcat cattttggca aagaa 
//
