LOCUS       pCAGGS-F-V278A          6457 bp    DNA     circular     17-AUG-2018
DEFINITION  LMBP 10256.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10256
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            
            
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12425257|
COMMENT     VNTDBDATE|-12425259|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pCAGGS-F-V278A|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark-Zwijnaarde 71|
COMMENT     VNTOAUTHORAD3|B-9052 Gent Belgium|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     VNTEXTCHREPL|Animal/Other Eukaryotic
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #2=(CFSignal (CObList) "lac promoter" 30 0 1 2273 2355 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(617..699)") 
COMMENT        #3=(CFSignal (CObList) "SV40 ori" 33 0 0 2497 2572 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "841..916") 
COMMENT        #4=(CFSignal (CObList) "SV40 polyA early" 43 0 0 2603 2677 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "947..1021") 
COMMENT        #5=(CFSignal (CObList) "SV40 polyA late" 43 0 1 2603 2677 0 
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COMMENT        #6=(CFSignal (CObList) "pMB1 ori" 33 0 1 2792 3193 0 (CStringList) 
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COMMENT        #7=(CFSignal (CObList) "ampicillin resistance" 4 0 1 3734 4594 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(2078..2938)") 
COMMENT        #8=(CFSignal (CObList) "hCMV-IE enhancer" 29 0 0 4730 5107 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "3074..3451") 
COMMENT        #9=(CFSignal (CObList) "AG promoter" 29 0 0 5113 6454 0 (CStringList) 
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COMMENT        #10=(CFSignal (CObList) "intron AG promoter" 15 0 0 5482 6406 0 
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COMMENT        #12=(CFSignal (CObList) "V278A" 62 0 0 849 851 0 (CStringList) 
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COMMENT                          "DNA Plasmid 'pCAGGS-F-V278A'" 1) 
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COMMENT                          "LMBP 10256." 1) 
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COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 
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COMMENT                          "Created: 08/17/18 02:52" 1) 
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COMMENT                     #21=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 6457 bp" 1) 
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COMMENT                                   -6.96932 0 -10 8.03237 1.16519 #236#) 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "F mutant" "@N" 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "intron AG promoter" "@N" 1 -10.568 10.454 0 
COMMENT                                   -10 7.71008 1.16519 #241#)) (CObjectList)) 
COMMENT                      #243=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil -959 100) 
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COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "3' flanking region of rabbit \337-globin" 
COMMENT                                   "@N" 1 18.6426 -5.22699 0 -10 13.8087 1.16519 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "hCMV-IE enhancer" "@N" 1 -16.1134 1.74233 0 
COMMENT                                   -10 7.53654 1.16519 #247#) 
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COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #9# 0) 1 2 0 0 #166# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    0.0029146 1.30671 1 0.082322) 0.8 1.8 0) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
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COMMENT                                    "Arial") 1.74233 0.666667 0 "AG promoter" 
COMMENT                                   "@N" 1 -11.4633 8.71165 0 -10 5.23096 1.16519 
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COMMENT                      #251=(CGroupWidget 
COMMENT                            (CWidget 30 (7 30 0) 1 2 0 0 Nil -608 100) 
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COMMENT                                    (CWidget 0 (3 #2# 0) 1 2 0 0 #190# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 2 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    3.98523 4.06586 1 0.082322) 0.8 1.8 1) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "lac promoter" 
COMMENT                                   "@N" 1 12.7756 -6.96932 0 -10 5.05742 1.16519 
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COMMENT                      #254=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil -1099 100) 
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COMMENT                                    (CWidget 0 (3 #3# 0) 1 2 0 0 #115# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    3.7744 3.84824 1 0.082322) 0.8 1.8 0) 
COMMENT                             #256=(CLabel (CWidget 0 (0 0) 1 2 0 0 #179# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "SV40 ori" "@N" 
COMMENT                                   1 10.6183 -8.71165 0 -10 3.56994 1.16519 
COMMENT                                   #255#) 
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COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    3.17108 3.56164 1 0.082322) 0.8 1.8 1) 
COMMENT                             #258=(CLabel (CWidget 0 (0 0) 1 2 0 0 #179# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pMB1 ori" "@N" 
COMMENT                                   1 5.52834 -13.9386 0 -10 3.56994 1.16519 
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COMMENT                      #259=(CGroupWidget 
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COMMENT                                   4.57689) 
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COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "3' UTR of rabbit \337-globin, incl. polyA" 
COMMENT                                   "@N" 1 19.5658 -1.74233 0 -10 14.3046 1.16519 
COMMENT                                   #260#) 
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COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "SV40 polyA early" "@N" 1 10.8118 -10.454 0 
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COMMENT                                   "SV40 polyA late" "@N" 1 10.6329 -12.1963 0 
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COMMENT                                   (LOGPEN 0 8 8388608) 10 1 5.44933 1.9 
COMMENT                                   0.082322 1) 
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COMMENT                                   (LOGPEN 0 0 32896) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.611111 0 "V278A" "@N" 1 
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COMMENT                     (CObjectList) (CObjectList)) 
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COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 0 
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FEATURES             Location/Qualifiers
     terminator      1747..1838
                     /vntifkey="43"
                     /label=3'\UTR\of\rabbit\ß-globin,\incl.\polyA
     misc_feature    1839..2195
                     /vntifkey="21"
                     /label=3'\flanking\region\of\rabbit\ß-globin
     promoter        complement(2273..2355)
                     /vntifkey="30"
                     /label=lac\promoter
                     /note="E. coli lac operon promoter"
     rep_origin      2497..2572
                     /vntifkey="33"
                     /label=SV40\ori
                     /note="bidirectional origin of SV40"
     terminator      2603..2677
                     /vntifkey="43"
                     /label=SV40\polyA\early
                     /note="polyA signal of the SV40 early region"
     terminator      complement(2603..2677)
                     /vntifkey="43"
                     /label=SV40\polyA\late
                     /note="polyA signal of the SV40 late region"
     rep_origin      complement(2792..3193)
                     /vntifkey="33"
                     /label=pMB1\ori
                     /note="origin plasmid"
     CDS             complement(3734..4594)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     promoter        4730..5107
                     /vntifkey="29"
                     /label=hCMV-IE\enhancer
                     /note="human CMV-IE enhancer"
     promoter        5113..6454
                     /vntifkey="29"
                     /label=AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid promoter"
     intron          5482..6406
                     /vntifkey="15"
                     /label=intron\AG\promoter
                     /note="chicken ß-actin/rabbit ß-globin hybrid intron"
     CDS             18..1745
                     /vntifkey="4"
                     /label=F\mutant
     mutation        849..851
                     /vntifkey="62"
                     /label=V278A
                     /note="WT: GTG"
BASE COUNT     1441 a      1770 c      1790 g      1456 t 
ORIGIN
        1 ttcgcggccg cgccaccatg gagctgccta tcctgaaggc caacgccatc accacaattc 
       61 tggccgccgt gaccttctgt tttgccagca gccagaacat caccgaggag ttctaccaga 
      121 gcacctgtag cgccgtgagc aagggctatc tgagcgccct gagaaccggc tggtacacca 
      181 gcgtgatcac catcgagctg agcaacatca aggagaacaa gtgcaacggc accgacgcca 
      241 aggtgaagct gatgaagcag gagctggaca agtacaagaa cgccgtgacc gaactgcagc 
      301 tgctgatgca gtctacccct gccgccaaca acagagccag acgggagctg ccccggttca 
      361 tgaactacac cctgaacaac accaagaaaa ccaacgtgac cctgagcaag aagcggaagc 
      421 ggagattcct gggctttctg ctgggagtgg gctctgccat cgcctctggc atcgccgtgt 
      481 ctaaggtgct gcacctggag ggagaggtga acaagatcaa gagcgccctg ctgagcacca 
      541 ataaggccgt ggtgagcctg agcaatggcg tgagcgtgct gacaagcaag gtgctggacc 
      601 tcaagaacta catcgacaag cagctgctgc ccatcgtgaa caagcagagc tgccggatca 
      661 gcaacatcga gaccgtgatc gagttccagc agaagaacaa ccggctgctg gagatcacca 
      721 gggagttcag cgtgaatgtg ggcgtgacca cccctgtgag cacctacatg ctgaccaaca 
      781 gcgagctgct gagcctgatc aacgacatgc ccatcaccaa cgaccagaag aagctgatgt 
      841 ccaacaacgc gcagatcgtg cggcagcaga gctacagcat catgtccatc atcaaggagg 
      901 aggtgctggc ttacgtggtg cagctgcctc tgtacggcgt gatcgacacc ccttgctgga 
      961 agctgcacac cagccctctg tgcaccacca ataccaagga gggcagcaac atctgcctga 
     1021 ccaggaccga tagaggctgg tactgcgaca atgccggcag cgtgagcttc tttccacagg 
     1081 ccgagacctg taaggtgcag agcaaccggg tgttctgcga caccatgaac agcctgaccc 
     1141 tgccttctga ggtgaacctg tgcaacgtgg acatcttcaa ccccaagtac gactgcaaga 
     1201 tcatgaccag caagaccgac gtgagcagca gcgtgattac aagcctgggc gccatcgtga 
     1261 gctgttacgg caagaccaag tgcaccgcca gcaacaagaa ccgcggcatc atcaagacct 
     1321 tcagcaacgg ctgcgactac gtgagcaaca agggcgtgga tacagtgagc gtgggcaaca 
     1381 ccctgtacta cgtcaacaag caggagggca agagcctgta cgtgaagggc gagcccatca 
     1441 tcaacttcta cgaccccctg gtgttcccta gcgacgagtt cgatgccagc atcagccagg 
     1501 tgaacgagaa gatcaaccag agcctggcct tcatcaggaa gagcgacgag ctgctgcaca 
     1561 atgtgaacgc cggcaagagc accaccaaca tcatgatcac caccatcatc atcgtgatca 
     1621 tcgtcatcct gctgtccctg attgctgtgg gcctgctgct gtactgtaag gccagaagca 
     1681 cccccgtgac cctgtctaag gatcagctga gcggcatcaa caacatcgcc ttctccaact 
     1741 gataaagatc tttttccctc tgccaaaaat tatggggaca tcatgaagcc ccttgagcat 
     1801 ctgacttctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg gaattttttg 
     1861 tgtctctcac tcggaaggac atatgggagg gcaaatcatt taaaacatca gaatgagtat 
     1921 ttggtttaga gtttggcaac atatgcccat atgctggctg ccatgaacaa aggttggcta 
     1981 taaagaggtc atcagtatat gaaacagccc cctgctgtcc attccttatt ccatagaaaa 
     2041 gccttgactt gaggttagat tttttttata ttttgttttg tgttattttt ttctttaaca 
     2101 tccctaaaat tttccttaca tgttttacta gccagatttt tcctcctctc ctgactactc 
     2161 ccagtcatag ctgtccctct tctcttatgg agatccctcg acctgcagcc caagcttggc 
     2221 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa 
     2281 catacgagcc ggaagcataa agtgtaaagc ctgggtgcct aatgagtgag ctaactcaca 
     2341 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagcggatc 
     2401 cgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa 
     2461 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag 
     2521 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag 
     2581 gcctaggctt ttgcaaaaag ctaacttgtt tattgcagct tataatggtt acaaataaag 
     2641 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt 
     2701 gtccaaactc atcaatgtat cttatcatgt ctggatccgc tgcattaatg aatcggccaa 
     2761 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg 
     2821 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg 
     2881 ttatccacag aatcagggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg 
     2941 ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg 
     3001 agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat 
     3061 accaggcgtt tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta 
     3121 ccggatacct gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct 
     3181 gtaggtatct cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc 
     3241 ccgttcagcc cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa 
     3301 gacacgactt atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg 
     3361 taggcggtgc tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag 
     3421 tatttggtat ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt 
     3481 gatccggcaa acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta 
     3541 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc 
     3601 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca 
     3661 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa 
     3721 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat 
     3781 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct 
     3841 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt 
     3901 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat 
     3961 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta 
     4021 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg 
     4081 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt 
     4141 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg 
     4201 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg 
     4261 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc 
     4321 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa 
     4381 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac 
     4441 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt 
     4501 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg 
     4561 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa 
     4621 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata 
     4681 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgggtcg acattgatta 
     4741 ttgactagtt attaatagta atcaattacg gggtcattag ttcatagccc atatatggag 
     4801 ttccgcgtta cataacttac ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc 
     4861 ccattgacgt caataatgac gtatgttccc atagtaacgc caatagggac tttccattga 
     4921 cgtcaatggg tggagtattt acggtaaact gcccacttgg cagtacatca agtgtatcat 
     4981 atgccaagta cgccccctat tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc 
     5041 cagtacatga ccttatggga ctttcctact tggcagtaca tctacgtatt agtcatcgct 
     5101 attaccatgg tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc 
     5161 ccacccccaa ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg 
     5221 gggggggggg ggcccccccc aggcggggcg gggcggggcg aggggcgggg cggggcgagg 
     5281 cggaaaggtg cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg 
     5341 aggcggcggc ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgttgcg 
     5401 cgctgccttc cccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga 
     5461 ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat 
     5521 tagcgcttgg tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg 
     5581 ctccgggagg gccctttgtg cggggggagc ggctcggggg gtgcgtgcgt gtgtgtgtgc 
     5641 gtggggagcg ccgcgtgcgg ctccgcgctg cccggcggct gtgagcgctg cgggcgcggc 
     5701 gcggggcttt gtgcgctccg cagtgtgcgc gaggggagcg cggccggggg cggtgccccg 
     5761 cggtgcgggg ggggctgcga ggggaacaaa ggctgcgtgc ggggtgtgtg cgtggggggg 
     5821 tgagcagggg gtgtgggcgc gtcggtcggg ctgcaacccc ccctgcaccc ccctccccga 
     5881 gttgctgagc acggcccggc ttcgggtgcg gggctccgta cggggcgtgg cgcggggctc 
     5941 gccgtgccgg gcggggggtg gcggcaggtg ggggtgccgg gcggggcggg gccgcctcgg 
     6001 gccggggagg gctcggggga aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg 
     6061 cgcggcgagc cgcagccatt gccttttatg gtaatcgtgc gagagggcgc agggacttcc 
     6121 tttgtcccaa atctgtgcgg agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg 
     6181 gcgcggggcg aagcggtgcg gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc 
     6241 gtcgccgcgc cgccgtcccc ttctccctct ccagcctcgg ggctgtccgc ggggggacgg 
     6301 ctgccttcgg gggggacggg gcagggcggg gttcggcttc tggcgtgtga ccggcggctc 
     6361 tagagcctct gctaaccatg ttcatgcctt cttctttttc ctacagctcc tgggcaacgt 
     6421 gctggttatt gtgctgtctc atcattttgg caaagaa 
//
