LOCUS       pEXPR-myr-FLAG-hTRIM2        7784 bp    DNA     circular      1-JUN-2016
DEFINITION  LMBP 10358.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10358
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11214150|
COMMENT     VNTDBDATE|-11263028|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEXPR-myr-FLAG-hTRIM2|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "DNA 'pEXPR-myr-FLAG-hTRIM2'" 1) 
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COMMENT                          "Created: 04/28/16 01:45" 1) 
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FEATURES             Location/Qualifiers
     CDS             complement(6788..7648)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             4487..5281
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      5457..5531
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(5457..5531)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(5845..6247)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(5670..5753)
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     rep_origin      4320..4395
                     /vntifkey="33"
                     /label=SV40\ori
     rep_origin      3649..4077
                     /vntifkey="33"
                     /label=f1\ori
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     968..991
                     /vntifkey="22"
                     /label=FLAG\epitope
     terminator      3379..3603
                     /vntifkey="43"
                     /label=BGH\polyA
     promoter        4082..4363
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             914..952
                     /vntifkey="4"
                     /label=mLck\myristoylation-targeting\sequence
     CDS             1082..3315
                     /vntifkey="4"
                     /label=hTRIM2
     attenuator      1058..1081
                     /vntifkey="0"
                     /label=AttB1
     attenuator      3316..3339
                     /vntifkey="0"
                     /label=attB2
BASE COUNT     1897 a      1993 c      2007 g      1887 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 
      901 taagcttggt accatgggct gtgtctgcag ctcaaaccct gaagatgact gggatcagct 
      961 taccatggat tacaaggacg atgacgataa ggtacctaag gatccagtgt ggtggaattc 
     1021 tgcagatatc cagcacagtg gcggccgctc gagcatcaca agtttgtaca aaaaagttgg 
     1081 catggccagt gaaggcacca acatcccaag tcctgtggtg cgccagattg acaagcagtt 
     1141 tctgatttgc agtatatgcc tggaacggta caagaatccc aaggttctcc cctgtctgca 
     1201 cactttctgc gagaggtgcc tgcagaacta cattcctgcc cacagtttaa ccctctcctg 
     1261 cccagtgtgc cgccagacct ccatcctgcc cgagaaaggg gtggccgcgc tccagaacaa 
     1321 tttcttcatc acaaacctga tggacgtgct gcagcgaact ccaggcagca acgctgagga 
     1381 gtcttccatc ctggagacag tcactgctgt ggctgcggga aagcctctct cttgcccaaa 
     1441 ccacgatggg aatgtgatgg aattttactg ccagtcctgt gagactgcca tgtgtcggga 
     1501 gtgcacggag ggggagcacg cagagcaccc cacagttcca ctcaaggatg tggtggaaca 
     1561 gcacaaggcc tcgctccagg tccagctgga tgctgtcaac aaaaggctcc cagaaataga 
     1621 ttctgctctt cagttcatct ctgaaatcat tcatcagtta accaaccaaa aggccagcat 
     1681 cgtggatgac attcattcca cctttgatga gctccagaag actttaaatg tgcgcaagag 
     1741 tgtgctgctt atggaattgg aggtcaacta tggcctcaaa cacaaagtcc tccagtcgca 
     1801 gctggatact ctgctccagg ggcaggagag cattaagagc tgcagcaact tcacagcgca 
     1861 ggccctcaac catggcacgg agaccgaggt cctactggtg aagaagcaga tgagcgagaa 
     1921 gctgaacgag ctggccgacc aggacttccc cttgcacccg cgggagaacg accagctgga 
     1981 tttcatcgtg gaaaccgagg ggctgaagaa gtccatccac aacctcggga cgatcttaac 
     2041 caccaacgcc gttgcctcag agacagtggc cacgggcgag gggctgcggc agaccatcat 
     2101 cgggcagccc atgtccgtca ccatcaccac caaggacaaa gacggtgagc tgtgcaaaac 
     2161 cggcaacgcc tacctcaccg ccgaactgag cacccccgac gggagcgtgg cagacgggga 
     2221 gatcctggac aacaagaacg gcacctatga gtttttgtac actgtccaga aggaagggga 
     2281 ctttaccctg tctctgagac tctatgacca gcacatccga ggcagcccgt ttaagctgaa 
     2341 agtgatccga tccgctgatg tgtctcccac cacagaaggc gtgaagaggc gcgttaagtc 
     2401 cccggggagc ggccacgtca agcagaaagc tgtgaaaaga cccgcaagca tgtacagcac 
     2461 tggaaaacga aaagagaatc ccatcgaaga cgatttgatc tttcgagtgg gtaccaaagg 
     2521 aagaaataaa ggagagttta caaatcttca gggggtagct gcatctacaa atggaaagat 
     2581 attaattgca gacagtaaca accaatgtgt gcagatattt tccaatgatg gccagttcaa 
     2641 aagtcgtttt ggcatacggg gacgctctcc ggggcagctg cagcggccca caggagtggc 
     2701 tgtacatccc agtggggaca taatcattgc cgattatgat aataaatggg tcagcatttt 
     2761 ctcctccgat gggaaattta agacaaaaat tggatcagga aagctgatgg gacccaaagg 
     2821 agtttctgtg gaccgcaatg ggcacattat tgttgtggac aacaaggcgt gctgcgtgtt 
     2881 tatcttccag ccaaacggga aaatagtcac caggtttggt agccgaggaa atggggacag 
     2941 gcagtttgca ggtccccatt ttgcagctgt aaatagcaat aatgagatta ttattacaga 
     3001 tttccataat cattctgtca aggtgtttaa tcaggaagga gaattcatgt tgaagtttgg 
     3061 ctcaaatgga gaaggaaatg ggcagtttaa tgctccaaca ggtgtagcag tggattcaaa 
     3121 tggaaacatc attgtggccg actggggaaa cagcaggatc caggtttttg atgggagtgg 
     3181 atcatttttg tcctacatta acacatctgc tgacccactc tatggccccc aaggcctggc 
     3241 cctaacttca gatggtcatg ttgtggttgc agactctgga aatcactgtt tcaaagtcta 
     3301 tcgatactta cagtacccaa ctttcttgta caaagtggtg atctagaggg cccgtttaaa 
     3361 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc 
     3421 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg 
     3481 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg 
     3541 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta 
     3601 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta 
     3661 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca 
     3721 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct 
     3781 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc 
     3841 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat 
     3901 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc 
     3961 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc 
     4021 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat 
     4081 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag 
     4141 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc 
     4201 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct 
     4261 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg 
     4321 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa 
     4381 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat 
     4441 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga 
     4501 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc 
     4561 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc 
     4621 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc 
     4681 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac 
     4741 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc 
     4801 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac 
     4861 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg 
     4921 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct 
     4981 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt 
     5041 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg 
     5101 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac 
     5161 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg 
     5221 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg 
     5281 agcgggactc tggggttcgc gaaatgaccg accaagcgac gcccaacctg ccatcacgag 
     5341 atttcgattc caccgccgcc ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg 
     5401 ccggctggat gatcctccag cgcggggatc tcatgctgga gttcttcgcc caccccaact 
     5461 tgtttattgc agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata 
     5521 aagcattttt ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc 
     5581 atgtctgtat accgtcgacc tctagctaga gcttggcgta atcatggtca tagctgtttc 
     5641 ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt 
     5701 gtaaagcctg gggtgcctaa tgagtgagct aactcacatt aattgcgttg cgctcactgc 
     5761 ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg 
     5821 ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac tcgctgcgct 
     5881 cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca 
     5941 cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga 
     6001 accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc 
     6061 acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg 
     6121 cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat 
     6181 acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt 
     6241 atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc 
     6301 agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg 
     6361 acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg 
     6421 gtgctacaga gttcttgaag tggtggccta actacggcta cactagaaga acagtatttg 
     6481 gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg 
     6541 gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca 
     6601 gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga 
     6661 acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga 
     6721 tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt 
     6781 ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt 
     6841 catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat 
     6901 ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag 
     6961 caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct 
     7021 ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt 
     7081 tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg 
     7141 cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca 
     7201 aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt 
     7261 tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat 
     7321 gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac 
     7381 cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc agaactttaa 
     7441 aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt 
     7501 tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt 
     7561 tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa 
     7621 gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt 
     7681 atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa 
     7741 taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtc 
//

