LOCUS       pEXPR-myr-FLAG-hTRIM48        6176 bp    DNA     circular      2-JUN-2016
DEFINITION  LMBP 10373.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10373
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11214269|
COMMENT     VNTDBDATE|-11264448|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEXPR-myr-FLAG-hTRIM48|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "DNA 'pEXPR-myr-FLAG-hTRIM48'" 1) 
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FEATURES             Location/Qualifiers
     CDS             complement(5180..6040)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             2879..3673
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      3849..3923
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(3849..3923)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(4237..4639)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(4062..4145)
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     rep_origin      2712..2787
                     /vntifkey="33"
                     /label=SV40\ori
     rep_origin      2041..2469
                     /vntifkey="33"
                     /label=f1\ori
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     968..991
                     /vntifkey="22"
                     /label=FLAG\epitope
     terminator      1771..1995
                     /vntifkey="43"
                     /label=BGH\polyA
     promoter        2474..2755
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             914..952
                     /vntifkey="4"
                     /label=mLck\myristoylation-targeting\sequence
     CDS             1082..1707
                     /vntifkey="4"
                     /label=hTRIM48
     attenuator      1058..1081
                     /vntifkey="0"
                     /label=attB1
     attenuator      1708..1731
                     /vntifkey="0"
                     /label=attB2
BASE COUNT     1465 a      1588 c      1573 g      1550 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 
      901 taagcttggt accatgggct gtgtctgcag ctcaaaccct gaagatgact gggatcagct 
      961 taccatggat tacaaggacg atgacgataa ggtacctaag gatccagtgt ggtggaattc 
     1021 tgcagatatc cagcacagtg gcggccgctc gagcatcaca agtttgtaca aaaaagttgg 
     1081 catgaattct ggaatctcgc aagtcttcca gagggaactc acctgcccca tctgcatgaa 
     1141 ctacttcata gacccggtca ccatagactg tgggcacagc ttttgcaggc cctgtttcta 
     1201 cctcaactgg caagacatcc caattcttac tcagtgcttt gaatgcataa agacaataca 
     1261 gcagagaaac ctcaaaacta acattcgatt gaagaagatg gcttcccttg ccagaaaagc 
     1321 cagtctctgg ctattcctga gctctgagga gcaaatgtgt ggcattcaca gggagacaaa 
     1381 gaagatgttc tgtgaagtgg acaggagcct gctctgtttg ctgtgctcca gctctcagga 
     1441 gcaccggtat cacagacact gtcccgctga gtgggctgct gaggaacact gggagaagct 
     1501 gttaaagaaa atgcagtctt tatgggaaaa agcttgtgaa aatcagagaa acctgaatgt 
     1561 ggaaaccacc agaatcagcc actggaaggc ttttggagac atattataca ggagtgagtc 
     1621 cgtgctgctg cacatgcccc agcctctgaa tctagcgctc agggcagggc ccatcactgg 
     1681 actgagggac aggctcaacc aattctgccc aactttcttg tacaaagtgg tgatctagag 
     1741 ggcccgttta aacccgctga tcagcctcga ctgtgccttc tagttgccag ccatctgttg 
     1801 tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact gtcctttcct 
     1861 aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt ctggggggtg 
     1921 gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat gctggggatg 
     1981 cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctctagg gggtatcccc 
     2041 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg 
     2101 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca 
     2161 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta 
     2221 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc 
     2281 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg 
     2341 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat 
     2401 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta 
     2461 acgcgaatta attctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc 
     2521 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc 
     2581 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat 
     2641 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc 
     2701 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct ctgcctctga 
     2761 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctccc 
     2821 gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat cgtttcgcat 
     2881 gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga ggctattcgg 
     2941 ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc ggctgtcagc 
     3001 gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga atgaactgca 
     3061 ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg cagctgtgct 
     3121 cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc cggggcagga 
     3181 tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg atgcaatgcg 
     3241 gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga aacatcgcat 
     3301 cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc tggacgaaga 
     3361 gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca tgcccgacgg 
     3421 cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg tggaaaatgg 
     3481 ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct atcaggacat 
     3541 agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg accgcttcct 
     3601 cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc gccttcttga 
     3661 cgagttcttc tgagcgggac tctggggttc gcgaaatgac cgaccaagcg acgcccaacc 
     3721 tgccatcacg agatttcgat tccaccgccg ccttctatga aaggttgggc ttcggaatcg 
     3781 ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg gagttcttcg 
     3841 cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa 
     3901 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca 
     3961 atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg taatcatggt 
     4021 catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac atacgagccg 
     4081 gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca ttaattgcgt 
     4141 tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat taatgaatcg 
     4201 gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc tcgctcactg 
     4261 actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca aaggcggtaa 
     4321 tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca aaaggccagc 
     4381 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc 
     4441 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat 
     4501 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc 
     4561 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct 
     4621 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg 
     4681 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc 
     4741 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga 
     4801 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa 
     4861 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta 
     4921 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc 
     4981 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg 
     5041 acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga 
     5101 tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg 
     5161 agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct 
     5221 gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg 
     5281 agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc 
     5341 cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa 
     5401 ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc 
     5461 cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt 
     5521 cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc 
     5581 ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt 
     5641 tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc 
     5701 catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt 
     5761 gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata 
     5821 gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga 
     5881 tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag 
     5941 catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa 
     6001 aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt 
     6061 attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga 
     6121 aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct gacgtc 
//

