LOCUS       pEXPR-myr-FLAG-hTRIM74        6299 bp    DNA     circular      2-JUN-2016
DEFINITION  LMBP 10380.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10380
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-11214326|
COMMENT     VNTDBDATE|-11264551|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pEXPR-myr-FLAG-hTRIM74|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT                          "DNA 'pEXPR-myr-FLAG-hTRIM74'" 1) 
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                          "Created: 04/28/16 04:41" 1) 
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FEATURES             Location/Qualifiers
     CDS             complement(5303..6163)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     CDS             3002..3796
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     terminator      3972..4046
                     /vntifkey="43"
                     /label=SV40\polyA\early
     terminator      complement(3972..4046)
                     /vntifkey="43"
                     /label=SV40\polyA\late
     rep_origin      complement(4360..4762)
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        complement(4185..4268)
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        235..818
                     /vntifkey="29"
                     /label=hCMV-IE\promoter\and\enhancer
     rep_origin      2835..2910
                     /vntifkey="33"
                     /label=SV40\ori
     rep_origin      2164..2592
                     /vntifkey="33"
                     /label=f1\ori
     promoter        863..879
                     /vntifkey="30"
                     /label=T7\promoter
     misc_marker     968..991
                     /vntifkey="22"
                     /label=FLAG\epitope
     terminator      1894..2118
                     /vntifkey="43"
                     /label=BGH\polyA
     promoter        2597..2878
                     /vntifkey="29"
                     /label=SV40\early\promoter
     CDS             914..952
                     /vntifkey="4"
                     /label=mLck\myristoylation-targeting\sequence
     CDS             1082..1830
                     /vntifkey="4"
                     /label=hTRIM74
     attenuator      1831..1854
                     /vntifkey="0"
                     /label=attB2
     attenuator      1058..1081
                     /vntifkey="0"
                     /label=attB1
BASE COUNT     1428 a      1670 c      1656 g      1545 t 
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg 
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt 
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagt 
      901 taagcttggt accatgggct gtgtctgcag ctcaaaccct gaagatgact gggatcagct 
      961 taccatggat tacaaggacg atgacgataa ggtacctaag gatccagtgt ggtggaattc 
     1021 tgcagatatc cagcacagtg gcggccgctc gagcatcaca agtttgtaca aaaaagttgg 
     1081 catggcttgg caggtgagcc tgctggagct ggaggaccgg cttcagtgtc ccatctgcct 
     1141 ggaggtcttc aaggagtccc taatgctaca gtgcggccac tcctactgca agggctgcct 
     1201 ggtttccctg tcctaccacc tggacaccaa ggtgcgctgc cccatgtgct ggcaggtggt 
     1261 ggacggcagc agctccttgc ccaacgtctc cctggcctgg gtgatcgaag ccctgaggct 
     1321 ccctggggac ccggagccca aggtctgcgt gcaccaccgg aacccgctca gccttttctg 
     1381 cgagaaggac caggagctca tctgtggcct ctgcggtctg ctgggctccc accaacacca 
     1441 cccggtcacg cccgtctcca ccatctgcag ccgcatgaag gaggagctcg cagccctctt 
     1501 ctctgagctg aagcaggagc agaagaaggt ggatgagctc atcgccaaac tggtgaacaa 
     1561 ccggacccga atcgtcaatg agtcggatgt cttcagctgg gtgatccgcc gcgagttcca 
     1621 ggagctgcgc cacccggtgg acgaggagaa ggcccgctgc ctggagggga tagggggtca 
     1681 cacccgtggc ctggtggcct ccctggacat gcagctggag caggcccagg gaacccggga 
     1741 gcggctggcc caagccgagt gtgtgctgga acagttcgga aatgaggacc accatgagtt 
     1801 catctggttc cactccatgg cctccaggta cccaactttc ttgtacaaag tggtgatcta 
     1861 gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc cagccatctg 
     1921 ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc actgtccttt 
     1981 cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct attctggggg 
     2041 gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg catgctgggg 
     2101 atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct agggggtatc 
     2161 cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga 
     2221 ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg 
     2281 ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat 
     2341 ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg 
     2401 ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata 
     2461 gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt 
     2521 tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat 
     2581 ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc 
     2641 cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa 
     2701 gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac 
     2761 catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc 
     2821 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctctgcctc 
     2881 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct 
     2941 cccgggagct tgtatatcca ttttcggatc tgatcaagag acaggatgag gatcgtttcg 
     3001 catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt 
     3061 cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc 
     3121 agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact 
     3181 gcaggacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt 
     3241 gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca 
     3301 ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat 
     3361 gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg 
     3421 catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga 
     3481 agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga 
     3541 cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa 
     3601 tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga 
     3661 catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt 
     3721 cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct 
     3781 tgacgagttc ttctgagcgg gactctgggg ttcgcgaaat gaccgaccaa gcgacgccca 
     3841 acctgccatc acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa 
     3901 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct 
     3961 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca 
     4021 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca 
     4081 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat 
     4141 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag 
     4201 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg 
     4261 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa 
     4321 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca 
     4381 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg 
     4441 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc 
     4501 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc 
     4561 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac 
     4621 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc 
     4681 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata 
     4741 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc 
     4801 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca 
     4861 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag 
     4921 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta 
     4981 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg 
     5041 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc 
     5101 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt 
     5161 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa 
     5221 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat 
     5281 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga 
     5341 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac 
     5401 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg 
     5461 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg 
     5521 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt 
     5581 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct 
     5641 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat 
     5701 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta 
     5761 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca 
     5821 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat 
     5881 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac 
     5941 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa 
     6001 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt 
     6061 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg 
     6121 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat 
     6181 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt 
     6241 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc 
//

