LOCUS       BiFC1                   9065 bp    DNA     circular SYN 24-NOV-2017
DEFINITION  LMBP 10464.
ACCESSION   .
VERSION     .
KEYWORDS    BiFC1.
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9065)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 4, 2017 from SnapGene 3.2.0
            http://www.snapgene.com
COMMENT     Accession number: LMBP 10464
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 24-NOV-2017
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDBDATE|-12042472|
COMMENT     LSOWNER|
COMMENT     VNTNAME|BiFC1|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "BiFC1" 0 9065 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
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COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
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COMMENT       (CMolPar 0 0 1 3446 3445 1 9065 0 0 0 0 0 0 0 0) (CStringList) 
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COMMENT        #1=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 3242 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 7592 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 7567 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BspEI" "tccgga" 2 0 1 6999 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 7584 0 0 "")) 
COMMENT       (CObList 
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COMMENT            (CStringList "/codon_start=1") 1 1 1 1 "321..2503") 
COMMENT        #7=(CFSignal (CObList) "integration sequence" 21 0 0 2731 3848 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2741..3858") 
COMMENT        #8=(CFSignal (CObList) "ccdB" 4 0 0 3858 4160 0 (CStringList) 
COMMENT            (CStringList "/codon_start=1" "/gene=\"ccdB\"" 
COMMENT             "/product=\"CcdB, a bacterial toxin that poisons DNA gyrase\"") 1 1 
COMMENT            1 1 "3868..4170") 
COMMENT        #9=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 0 4509 5303 0 
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COMMENT             "/product=\"aminoglycoside phosphotransferase from Tn5\"") 1 1 1 1 
COMMENT            "4519..5313") 
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COMMENT        #11=(CFSignal (CObList) "yEmVenus fragment" 4 0 0 7004 7519 0 
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COMMENT             (CStringList) (CStringList) 1 1 1 1 "7621..8134") 
COMMENT        #15=(CFSignal (CObList) "lac promoter" 30 0 0 85 168 0 (CStringList) 
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COMMENT        #16=(CFSignal (CObList) "SP6 promoter" 30 0 0 229 245 0 (CStringList) 
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COMMENT        #17=(CFSignal (CObList) "T7 promoter" 30 0 1 2588 2604 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #18=(CFSignal (CObList) "ampicillin resistance promoter" 30 0 0 5376 
COMMENT             5471 0 (CStringList) (CStringList) 1 1 1 1 "") 
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                          "Creation Date: ? (no data)" 1) 
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COMMENT                          "length: 9065 bp" 1) 
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COMMENT                          "Original Source Database: GenBank" 1) 
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COMMENT                          "Division: SYN" 1) 
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COMMENT                          "Modification Date in the Original DB: 18-DEC-2015" 1) 
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COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/.\">.</a>" 
COMMENT                          1) 
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COMMENT                          "Version in the Original DB: ." 1) 
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COMMENT                          "Sequence Source: synthetic DNA construct" 1) 
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COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/synthetic+DNA+construct\">synthetic DNA construct(synthetic DNA construct)</a>" 
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COMMENT                          "            http://www.snapgene.com" 1))) 
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FEATURES             Location/Qualifiers
     source          9056..9055
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /vntifkey="98"
     CDS             1175..2296
                     /codon_start=1
                     /vntifkey="4"
                     /label=CmLEU2
                     /note="Candida maltosa LEU2"
     misc_feature    2731..3848
                     /vntifkey="21"
                     /label=integration\sequence
     CDS             3858..4160
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /vntifkey="4"
                     /label=ccdB
     CDS             4509..5303
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     promoter        5558..6997
                     /vntifkey="29"
                     /label=CaMET3\promoter
     CDS             7004..7519
                     /codon_start=1
                     /vntifkey="4"
                     /label=yEmVenus\fragment
                     /note="yEmVenus aa1-172"
     misc_feature    7526..7555
                     /vntifkey="21"
                     /label=GGGGSGGGGS-linker
     misc_feature    7580..7582
                     /vntifkey="21"
                     /label=stop\codon
     terminator      7611..7883
                     /vntifkey="43"
                     /label=ACT1\terminator
     promoter        85..168
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        229..245
                     /vntifkey="30"
                     /label=SP6\promoter
     promoter        complement(2588..2604)
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        5376..5471
                     /vntifkey="30"
                     /label=ampicillin\resistance\promoter
     rep_origin      8656..9058
                     /vntifkey="33"
                     /label=pMB1\ori
BASE COUNT     2616 a      1819 c      1937 g      2693 t 
ORIGIN
        1 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 
       61 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 
      121 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg 
      181 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat ttaggtgaca 
      241 ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca ctagtaacgg 
      301 ccgccagtgt gctggaattc gcccttggat ccaatcatca ctggtggggg tcaagaaaga 
      361 ggtttgagat caggtacttt ggcaccacca ttagtagctg ggtttggtga agctgcaaga 
      421 ttaatgaaac aagaatctgc atttgacaag aaacatattg agaggttatc tactaaattg 
      481 aagaacggtt tgttatccat tccatctact cagtttaacg gttgtaataa cccaacatat 
      541 caatacccag gttgtgtgaa tgtttccttt gcatacattg aaggggaatc tttgttaatg 
      601 gcattgaaag atattgcatt gagttctggt tctgcatgta cctctgcatc tttggaacca 
      661 tcgtatgttt tacatgcttt gggtgccgat gatgccttgg ctcattcttc cattagattc 
      721 ggtattggta gattcaccac tgaagcagaa gttgactatg ttattcaagc aattaatgaa 
      781 agagttgatt tcttgagaaa gatgtctcca ttatgggaaa tggttcaaga aggtattgac 
      841 ttgaactcta tcgaatggag tggacattaa ggttttttgt ttttttgtaa tttgtgttgt 
      901 tatgtgtaat tatcgtgtat agagtaattt cggaaattgt actttcgttc atgtatctga 
      961 accagatgaa taatacgttc gtattgttga agaatgacta actcctgtaa cggcgtgcgt 
     1021 gggttccgca gccgggttca attttttttt tcttgttcac tataaaaata tataataaac 
     1081 gaaaaaaatc cgagggactc aggctatgaa atttttcact caagaatttt ttttccttca 
     1141 tccttaattc ctttattcac tacccaaacc cacaatgtca gttaaaacta aaactattac 
     1201 aattttacca ggtgatcacg tcggtaccga aattgtcaat gaagctatca aagttttaga 
     1261 agcaattgaa gctgccactc cttaccaaaa aatccatttc gatttcaaac atcatttaat 
     1321 cggtggtgct gccattgatg ccactggtgt cccattgcca gatgacgcac ttgaaagtgc 
     1381 caaaaaatct gatgccgtct tattgggtgc tgttggtgga ccaaaatggg ggactggcgc 
     1441 cgttcgtcca gaacaaggtt tattaaagat tcgtaaagaa ttgaaccttt atgctaacat 
     1501 cagaccatgt aactttgcca gtgactcctt attggaatta tccccattga gaccagaagt 
     1561 cgttaaaggt acaaacttga tcattgttcg tgaattagtt ggtggtattt atttcggtga 
     1621 tcgtgaagaa caagaagaaa gtgaagataa acaaaccgca tgggatactg aaaaatacac 
     1681 cgtcgatgaa gtcaccagaa tcacccgtat ggctgccttc atggctttac aacataaccc 
     1741 accattacca atctggtcat tggataaggc taatgtttta gcttcttcta gattatggag 
     1801 aagaactgtc gataaagtga tttctgaaga gttcccaact ttgtctgttc aacatcaatt 
     1861 gattgactcc gccgccatga ttttaattca aaacccaacc aaattgaatg gtataatcat 
     1921 cacttctaac atgtttggtg atatcatttc cgatgaagct tcagttattc caggttcctt 
     1981 gggtttatta ccatctgcat ctttggcttc attgccagat accaacaccg cttttggtct 
     2041 ttatgaacca tgtcacggtt ctgctcctga tttaccagcc aataaagtta acccaattgc 
     2101 tactatttta tctgctgctt ctatgttgag attatctttg gattgtgtga aagaagctga 
     2161 agctttagaa gaagccgtga aacaagtctt ggataaaggt atcagaactg cagatttaag 
     2221 aggtaccagt tcagctactg aagttggtga cgcgattgtc gaagctgtta ctaaaatctt 
     2281 aaaagaaaaa gcttaagtaa agtatacaca aaatttaacg aatattatta atacaaaatg 
     2341 aacaccacct aaatactttt ttttaaaaat gtaccgactc ttctaaaaat tgcgataatt 
     2401 caatatcgga aatttctctc accggtttac ttggatcttc ggaaataaat ctatttgaag 
     2461 taatggtagc ctcacttgat tctgattggc tctttctaga catgggtagg taagggcgaa 
     2521 ttctgcagat atccatcaca ctggcggccg gccgctcgag catgcatcta gagggcccaa 
     2581 ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg 
     2641 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg 
     2701 gcgtaatagc gaagaggccc gcaccgatcg accagatcaa aatgctagaa caaattacgt 
     2761 ctgcatcaac agtcatcttc cttatttgca taaatgctaa aatggcaaac ctgtaaaccg 
     2821 tttgaaaagc aaattgagat agttagctcg gttttttttt cacgtggcca taatccaaca 
     2881 aaaaaaagga atttaagggg ggagagaagg aggcttgaat ttgtaccaca gagcgagcaa 
     2941 gaaattaata atatacttgg cttagctgaa atgagaatga aaaagcaaat ttcaagtgaa 
     3001 acaccactac tagccaagta aattggccaa tttgctctcg tgctttaatt tcctaacaag 
     3061 cttttgagac cgaaaaaaat caaccaatga tgatggaaaa atacttccgg gagtcaattt 
     3121 atccataatt tcacgtcgtt ttttttttgc acatggcaaa tatattggaa tcctgagatt 
     3181 ttcttggagt cgtgtacaat aacaccaggc gggaacatgc cgttatgatc cagcatatgg 
     3241 cggccgccat atgtgttcaa atattgaaac cgtgaaacca gtttgataca gctgatattg 
     3301 tgtgctaaaa aaagatgagc accgaaagag tctttagttt attgccaagt aaagggtcaa 
     3361 agaataccta ttctttatga acacagactt gctctaatgg tgaattagtg tggagtaata 
     3421 gagaaggagg cgaaacaaaa ataattggtt aaacactcga atgtcacaca atcctaaata 
     3481 ggttaaattc agttaccgcc gtgatgcggg aacaaagccg agcggtaata tttttggatt 
     3541 ctatgttttt ttggtgtata ttgcacgact tggtgccaac aactggtgtt ccaacgcaca 
     3601 aaactaggtt caactggata ctgttttcta gaaattattt gcggcaattc atgattacgg 
     3661 tcctaaattt gaaataaaag tttccaatta tgtgtcttga caatttccat tgggttcaaa 
     3721 aagatctaga atgtatgtaa ttacgcaaca atttgcatgc aaagaaataa aaacaaacct 
     3781 aaaatccaac tgctgttgtt ctggtgatga tgtagtaaaa taagagcacg gccaagaaca 
     3841 aggtgattta cgtacggcag tttaaggttt acacctataa aagagagagc cgttatcgtc 
     3901 tgtttgtgga tgtacagagt gatattattg acacgccggg gcgacggatg gtgatccccc 
     3961 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata 
     4021 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta 
     4081 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc 
     4141 tgatgttctg gggaatataa atgtcaggca tgagattatc aaaaaggatc ttcacctaga 
     4201 tccttttcac gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct 
     4261 actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg 
     4321 ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc 
     4381 cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct 
     4441 cgccgccaag gatctgatgg cgcaggggat caagctctga tcaagagaca ggatgaggat 
     4501 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga 
     4561 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc 
     4621 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga 
     4681 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg 
     4741 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc 
     4801 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg 
     4861 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga 
     4921 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc 
     4981 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca 
     5041 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg 
     5101 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct 
     5161 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg 
     5221 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc 
     5281 gccttcttga cgagttcttc tgaattatta acgcttacaa tttcctgatg cggtattttc 
     5341 tccttacgca tctgtgcggt atttcacacc gcatacaggt ggcacttttc ggggaaatgt 
     5401 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 
     5461 acaataaccc tgataaatgc ttcaataata gcacgtgagg agggccacca tggccaagtt 
     5521 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcttc caattagagg ttgtggatgc 
     5581 aaaattgaaa aaaaaatgac tattggtagc gtgttctatt tttcttatgc gctgaaaaac 
     5641 tacgaacaat tgtctattcc aagcctgtgg ctttttttgg tgattcacaa tacataaatt 
     5701 ggcaaatacc tcttctatag actttaacct ttgatgaaat ggtatggata aaccactttt 
     5761 gtggtcaggg tatatatatt tttattccaa tctggccttt ataaattcaa atttccaagg 
     5821 ggactctgct aaaccttctg atataactat aactctaata atggctgtaa aaagtgtgaa 
     5881 aaaaagatga tattcacaaa aaagataaag gggatttcat attgggtatc tagatttaaa 
     5941 catcgtattt caacaatgaa actagaatca atacgaaatc cagggcacaa ctgtgttcat 
     6001 tcccttggga attatactga gtaatgggaa gaatgtgcaa aaaatacccg tgtcttatat 
     6061 aatttccatt tgtgtagggc ttaaaagtgg acaacttcag gttacgaaac cacgaaaatg 
     6121 tctaaacgtt tgatcaaatt cgtaactcat ggaaaatcac agcaacaaat taaggatgga 
     6181 tatatccggt acttacaaat aaaaacacta aagaagtata aattttgaac tgaaattcta 
     6241 tagaaaatct ttttttttgg gtaaaattat gcgattgtgg ctcatagtaa cgtaatcata 
     6301 cagttcaaca aacatcattt tgtcaaagga tttctaatcc tattcttttt ctacttggct 
     6361 tttgagtata tatatatata tatatacatc ctttgttcat catcatgttc gattgaattt 
     6421 ttttcttggt tgttattaga ttgtctactt gagtttttct tggtgcaaat tgtggtgttt 
     6481 ttctaacaaa tttttcaaca tttaaagtat ttctggaact tttctacaag gaacgacata 
     6541 gatccattaa tgcgcccctc taaaacaata cccataatgt cttcttaagt atgcatctgc 
     6601 ttataaatgt ttctggattt actttagtta gaatgtcgac acgacaatga actattgctg 
     6661 tagatcacgt gcgatcactg aattatgagt ttctttttat ttttcttttt gggctttttt 
     6721 ttttgcaatt ttttttttcc acctttgcca attgcgtagt cacatataat gtatacaatt 
     6781 tttgcaactc tgacactata attgatccaa taataaagta tataatagat catcaagtat 
     6841 acgtaatctc cccaaaaaaa aaattgcata aaataaaatc tataaacctt ttttagattt 
     6901 ttagttgttc cttttttctt tctattcttc aacttccaag tgttgtcact ttcttcctgt 
     6961 taactatatt taaaagtaaa taccctcccc agaaaactcc ggaatgtcta aaggtgaaga 
     7021 attattcact ggtgttgtcc caattttggt tgaattagat ggtgatgtta atggtcacaa 
     7081 attttctgtc tccggtgaag gtgaaggtga tgctacttac ggtaaattga ccttaaaatt 
     7141 gatttgtact actggtaaat tgccagttcc atggccaacc ttagtcacta ctttaggtta 
     7201 tggtttgcaa tgttttgcta gatacccaga tcatatgaaa caacatgact ttttcaagtc 
     7261 tgccatgcca gaaggttatg ttcaagaaag aactattttt ttcaaagatg acggtaacta 
     7321 caagaccaga gctgaagtca agtttgaagg tgatacctta gttaatagaa tcgaattaaa 
     7381 aggtattgat tttaaagaag atggtaacat tttaggtcac aaattggaat acaactataa 
     7441 ctctcacaat gtttacatca ctgctgacaa acaaaagaat ggtatcaaag ctaacttcaa 
     7501 aattagacac aacattgaag ctagcggagg tggaggttct ggtggaggtg gttcaagcct 
     7561 agggacccgg gggcgcgcct aaacgcgtag tacttaaccg cgccgtcgac gagtgaaatt 
     7621 ctggaaatct ggaaatctgg ttttgtattc ttgttattct tctttttgtt attacatata 
     7681 taacttgtta cttttttaaa aaaatctttg tttattttat aaatatataa aactaaattt 
     7741 aagaaaaaga gaaaaatgtt ttatttgaga gattgaaatt ttacttgaat ttagcttagc 
     7801 ttttataaag tattattatg taaaaaaaca aaacaaatat acattaaaaa gttaagacta 
     7861 taaaatagcc acccaaggca tttctatatc ttgttgttgt tgttttcatc ttctgtatca 
     7921 gaggaactta ttttattatt ttcgtcacgg gtattttctc ttgtttgatg attcatccca 
     7981 ttcattccat cataaaattc tagaaggacc acctttgatt gtaaatagta ataattacca 
     8041 cccttattta attatttatt taacttattt atttatttat tatacatata tacaaatcta 
     8101 ataaagtgaa aatctccccc ttcacacgtg gccgaggagc aggactgaca cgtgctaaaa 
     8161 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa 
     8221 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga 
     8281 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg 
     8341 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact 
     8401 ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac 
     8461 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg 
     8521 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg 
     8581 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga 
     8641 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc 
     8701 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg 
     8761 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc 
     8821 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc 
     8881 agcaacgcgg cctttttacg gttcctgggc ttttgctggc cttttgctca catgttcttt 
     8941 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc 
     9001 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc 
     9061 ccaat 
//
