LOCUS       BiFC2                   9068 bp    DNA     circular SYN 24-NOV-2017
DEFINITION  LMBP 10465.
ACCESSION   .
VERSION     .
KEYWORDS    BiFC2.
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9068)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 4, 2017 from SnapGene 3.2.0
            http://www.snapgene.com
COMMENT     Accession number: LMBP 10465
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 24-NOV-2017
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12032363|
COMMENT     VNTDBDATE|-12042466|
COMMENT     LSOWNER|
COMMENT     VNTNAME|BiFC2|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "BiFC2" 0 9068 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "NheI" "NotI" "ScaI" "XmaI" "BspEI" "MluI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 1 5327 5326 1 9068 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "NheI" "gctagc" 2 0 1 7062 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "NotI" "gcggccgc" 3 0 1 3242 0 0 "") 
COMMENT        #2=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 7595 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 7018 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BspEI" "tccgga" 2 0 1 6999 0 0 "") 
COMMENT        #5=(CRSite (CStringList) "MluI" "acgcgt" 2 0 1 7587 0 0 "")) 
COMMENT       (CObList 
COMMENT        #6=(CFSignal (CObList) "lac promoter" 30 0 0 85 168 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "143..173") 
COMMENT        #7=(CFSignal (CObList) "SP6 promoter" 30 0 0 229 245 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "239..257") 
COMMENT        #8=(CFSignal (CObList) "T7 promoter" 30 0 1 2588 2604 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2596..2614)") 
COMMENT        #9=(CFSignal (CObList) "integration sequence" 21 0 0 2731 3848 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "2741..3858") 
COMMENT        #10=(CFSignal (CObList) "ccdB" 4 0 0 3858 4160 0 (CStringList) 
COMMENT             (CStringList "/codon_start=1" "/gene=\"ccdB\"" 
COMMENT              "/product=\"CcdB, a bacterial toxin that poisons DNA gyrase\"") 1 
COMMENT             1 1 1 "3868..4170") 
COMMENT        #11=(CFSignal (CObList) "Tn5 neomycin resistance" 4 0 0 4509 5303 0 
COMMENT             (CStringList) 
COMMENT             (CStringList "/codon_start=1" "/gene=\"aph(3')-II (or nptII)\"" 
COMMENT              "/product=\"aminoglycoside phosphotransferase from Tn5\"") 1 1 1 1 
COMMENT             "4519..5313") 
COMMENT        #12=(CFSignal (CObList) "CaMET3 promoter" 29 0 0 5558 6997 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "5568..7007") 
COMMENT        #13=(CFSignal (CObList) "start codon" 21 0 0 7004 7006 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "7014..7016") 
COMMENT        #14=(CFSignal (CObList) "GGGGSGGGGS-linker" 21 0 0 7031 7060 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "7041..7070") 
COMMENT        #15=(CFSignal (CObList) "yEmVenus fragment" 4 0 0 7067 7585 0 
COMMENT             (CStringList) (CStringList "/codon_start=1") 1 1 1 1 "7077..7595") 
COMMENT        #16=(CFSignal (CObList) "ACT1 terminator" 43 0 0 7614 7886 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "7624..8137") 
COMMENT        #17=(CFSignal (CObList) "pMB1 ori" 33 0 0 8659 9061 0 (CStringList) 
COMMENT             (CStringList "/direction=RIGHT") 1 1 1 1 "8299..8887") 
COMMENT        #18=(CFSignal (CObList) "CmLEU2" 4 0 0 1175 2296 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #19=(CFSignal (CObList) "amp promoter" 30 0 0 5376 5471 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #20=(CFSignal (CObList) "LMBP 10465" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #21=(CFSignal (CObList) "Last modified: 24-NOV-2017" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
COMMENT        #22=(CGroupPar (CParagraph 0 (0 0) 1 2 0 0 180) 
COMMENT             (CObjectList 
COMMENT              #23=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "BiFC2" 2) 5 "" 0 
COMMENT                   4) 
COMMENT              #24=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 1 (0 0) 1 2 0 1 178) 
COMMENT                    (CObjectList 
COMMENT                     #25=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid 'BiFC2'" 1) 
COMMENT                     #26=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) "LMBP 10465" 
COMMENT                          1) 
COMMENT                     #27=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
COMMENT                     #28=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 11/17/17 05:38" 1) 
COMMENT                     #29=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 11/24/17 06:01" 1) 
COMMENT                     #30=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 9068 bp" 1) 
COMMENT                     #31=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
COMMENT                     #32=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "form: Circular" 1))) "General Description") 
COMMENT              #33=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 2 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Keywords: BiFC2" 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Source Database: GenBank" 1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Division: SYN" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Modification Date in the Original DB: 18-DEC-2015" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/.\">.</a>" 
COMMENT                          1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Version in the Original DB: ." 1) 
COMMENT                     #40=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Sequence Source: synthetic DNA construct" 1) 
COMMENT                     #41=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/synthetic+DNA+construct\">synthetic DNA construct(synthetic DNA construct)</a>" 
COMMENT                          1) 
COMMENT                     #42=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Associated Genes:  ccdB;  aph(3')-II (or nptII)" 1))) 
COMMENT                   "Standard Fields") 
COMMENT              #43=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 3 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "REFERENCE   1  (bases 1 to 9068)" 1) 
COMMENT                     #45=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "  AUTHORS   ." 1) 
COMMENT                     #46=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "  TITLE     Direct Submission" 1) 
COMMENT                     #47=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "  JOURNAL   Exported Friday, Aug 4, 2017 from SnapGene 3.2.0" 
COMMENT                          1) 
COMMENT                     #48=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "            http://www.snapgene.com" 1))) 
COMMENT                   "References") 
COMMENT              #49=(CRefLinePar 
COMMENT                   (CLinePar (CParagraph 0 (0 0) 0 2 0 0 233) "Comments" 2) 1 "" 
COMMENT                   0 0) 
COMMENT              #50=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 8 (0 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #51=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #52=(CLinePar (CParagraph 0 (15 #20# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "LMBP 10465") 
COMMENT                     #53=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 2 (16 0) 1 2 1 0 179) 
COMMENT                           (CObjectList 
COMMENT                            #54=(CLinePar (CParagraph 0 (15 #21# 0) 1 2 2 0 180) 
COMMENT                                 "" 1))) "Last modified: 24-NOV-2017"))) 
COMMENT                   "Annotations") 
COMMENT              #55=(CFolderPar 
COMMENT                   (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                    (CObjectList 
COMMENT                     #56=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 4 (7 4 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #57=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 1175 (3 #18# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #58=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 1175  End: 2296" 1) 
COMMENT                                   #59=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Candida maltosa LEU2" 1))) "CmLEU2") 
COMMENT                            #60=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 3858 (3 #10# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #61=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 3858  End: 4160" 1) 
COMMENT                                   #62=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #63=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   3868..4170" 1) 
COMMENT                                   #64=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Qualifiers:" 1) 
COMMENT                                   #65=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /codon_start=1" 1) 
COMMENT                                   #66=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /gene=\"ccdB\"" 1) 
COMMENT                                   #67=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /product=\"CcdB, a bacterial toxin that poisons DNA gyrase\"" 
COMMENT                                        1))) "ccdB") 
COMMENT                            #68=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 4509 (3 #11# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #69=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 4509  End: 5303" 1) 
COMMENT                                   #70=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #71=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   4519..5313" 1) 
COMMENT                                   #72=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Qualifiers:" 1) 
COMMENT                                   #73=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /codon_start=1" 1) 
COMMENT                                   #74=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /gene=\"aph(3')-II (or nptII)\"" 1) 
COMMENT                                   #75=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /product=\"aminoglycoside phosphotransferase from Tn5\"" 
COMMENT                                        1))) "Tn5 neomycin resistance") 
COMMENT                            #76=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 7067 (3 #15# 0) 1 2 2 0 327) 
COMMENT                                  (CObjectList 
COMMENT                                   #77=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7067  End: 7585" 1) 
COMMENT                                   #78=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "yEmVenus aa1-172" 1) 
COMMENT                                   #79=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #80=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   7077..7595" 1) 
COMMENT                                   #81=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Qualifiers:" 1) 
COMMENT                                   #82=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   /codon_start=1" 1))) 
COMMENT                                 "yEmVenus fragment"))) "CDS (4 total)") 
COMMENT                     #83=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 21 (7 21 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #84=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 2731 (3 #9# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #85=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 2731  End: 3848" 1) 
COMMENT                                   #86=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #87=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   2741..3858" 1))) 
COMMENT                                 "integration sequence") 
COMMENT                            #88=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 7004 (3 #13# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #89=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7004  End: 7006" 1) 
COMMENT                                   #90=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "START CODON" 1) 
COMMENT                                   #91=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #92=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   7014..7016" 1))) "start codon") 
COMMENT                            #93=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 7031 (3 #14# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #94=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 7031  End: 7060" 1) 
COMMENT                                   #95=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Original Location Description:" 1) 
COMMENT                                   #96=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "   7041..7070" 1))) "GGGGSGGGGS-linker"))) 
COMMENT                          "Misc. Feature (3 total)") 
COMMENT                     #97=(CFolderPar 
COMMENT                          (CGroupPar (CParagraph 29 (7 29 0) 1 1 1 0 178) 
COMMENT                           (CObjectList 
COMMENT                            #98=(CFolderPar 
COMMENT                                 (CGroupPar 
COMMENT                                  (CParagraph 5558 (3 #12# 0) 1 2 2 0 194) 
COMMENT                                  (CObjectList 
COMMENT                                   #99=(CLinePar 
COMMENT                                        (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                        "Start: 5558  End: 6997" 1) 
COMMENT                                   #100=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "Original Location Description:" 1) 
COMMENT                                   #101=(CLinePar 
COMMENT                                         (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                         "   5568..7007" 1))) "CaMET3 promoter"))) 
COMMENT                          "Promoter Eukaryotic (1 total)") 
COMMENT                     #102=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 30 (7 30 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #103=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 85 (3 #6# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #104=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 85    End: 168 " 1) 
COMMENT                                     #105=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #106=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   143..173" 1))) "lac promoter") 
COMMENT                             #107=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 229 (3 #7# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #108=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 229   End: 245 " 1) 
COMMENT                                     #109=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #110=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   239..257" 1))) "SP6 promoter") 
COMMENT                             #111=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 2588 (3 #8# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #112=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 2588  End: 2604 (Complementary)" 
COMMENT                                           1) 
COMMENT                                     #113=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #114=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   complement(2596..2614)" 1))) 
COMMENT                                   "T7 promoter") 
COMMENT                             #115=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 5376 (3 #19# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #116=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 5376  End: 5471" 1))) 
COMMENT                                   "amp promoter"))) 
COMMENT                           "Promoter Prokaryotic (4 total)") 
COMMENT                     #117=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 33 (7 33 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #118=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 8659 (3 #17# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #119=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 8659  End: 9061" 1) 
COMMENT                                     #120=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #121=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   8299..8887" 1) 
COMMENT                                     #122=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Qualifiers:" 1) 
COMMENT                                     #123=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   /direction=RIGHT" 1))) "pMB1 ori"))) 
COMMENT                           "Replication Origin (1 total)") 
COMMENT                     #124=(CFolderPar 
COMMENT                           (CGroupPar (CParagraph 43 (7 43 0) 1 1 1 0 178) 
COMMENT                            (CObjectList 
COMMENT                             #125=(CFolderPar 
COMMENT                                   (CGroupPar 
COMMENT                                    (CParagraph 7614 (3 #16# 0) 1 2 2 0 194) 
COMMENT                                    (CObjectList 
COMMENT                                     #126=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Start: 7614  End: 7886" 1) 
COMMENT                                     #127=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "Original Location Description:" 1) 
COMMENT                                     #128=(CLinePar 
COMMENT                                           (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                           "   7624..8137" 1))) 
COMMENT                                   "ACT1 terminator"))) "Terminator (1 total)"))) 
COMMENT                   "Feature Map") 
COMMENT              #129=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 12 (6 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #130=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 60 (0 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #131=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 311 (0 0) 1 2 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #132=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Start: 311   End: 2493" 1) 
COMMENT                                      #133=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "CmLEU2 CmLEU2:   C. maltosa LEU2 gene. Complements  leu2-Delta/leu2-Delta deletion of C. albicans" 
COMMENT                                            1) 
COMMENT                                      #134=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Original Location Description:" 1) 
COMMENT                                      #135=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   321..2503" 1))) "Gene_1"))) 
COMMENT                            "Gene (1 total)") 
COMMENT                      #136=(CFolderPar 
COMMENT                            (CGroupPar (CParagraph 98 (0 0) 1 1 1 0 178) 
COMMENT                             (CObjectList 
COMMENT                              #137=(CFolderPar 
COMMENT                                    (CGroupPar 
COMMENT                                     (CParagraph 9059 (0 0) 1 2 2 0 178) 
COMMENT                                     (CObjectList 
COMMENT                                      #138=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Start: 9059  End: 9058" 1) 
COMMENT                                      #139=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Original Location Description:" 1) 
COMMENT                                      #140=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   1..9068" 1) 
COMMENT                                      #141=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "Qualifiers:" 1) 
COMMENT                                      #142=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /organism=\"synthetic DNA construct\"" 
COMMENT                                            1) 
COMMENT                                      #143=(CLinePar 
COMMENT                                            (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                            "   /mol_type=\"other DNA\"" 1))) 
COMMENT                                    "Source_1"))) "Source (1 total)"))) 
COMMENT                    "Imported Features Not Shown on Map") 
COMMENT              #144=(CFolderPar 
COMMENT                    (CGroupPar (CParagraph 13 (0 0) 1 1 0 0 178) 
COMMENT                     (CObjectList 
COMMENT                      #145=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #146=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #0# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #147=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #0# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 7062 " 1))))) "NheI: 1 site") 
COMMENT                            1 5 "GCTAGC" "CGATCG") 
COMMENT                      #148=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #149=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #1# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #150=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #1# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 3242 " 1))))) "NotI: 1 site") 
COMMENT                            2 6 "GCGGCCGC" "CGCCGGCG") 
COMMENT                      #151=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #152=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #2# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #153=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #2# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 7595 " 1))))) "ScaI: 1 site") 
COMMENT                            3 3 "AGTACT" "TCATGA") 
COMMENT                      #154=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #155=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #3# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #156=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #3# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 7018 " 1))))) "XmaI: 1 site") 
COMMENT                            1 5 "CCCGGG" "GGGCCC") 
COMMENT                      #157=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #158=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #4# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #159=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #4# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 6999 " 1))))) "BspEI: 1 site") 
COMMENT                            1 5 "TCCGGA" "AGGCCT") 
COMMENT                      #160=(CRSFolderPar 
COMMENT                            (CFolderPar 
COMMENT                             (CGroupPar (CParagraph 1 (8 0) 1 1 1 0 178) 
COMMENT                              (CObjectList 
COMMENT                               #161=(CGroupPar 
COMMENT                                     (CParagraph 0 (10 #5# 0) 1 2 2 0 180) 
COMMENT                                     (CObjectList 
COMMENT                                      #162=(CLinePar 
COMMENT                                            (CParagraph 0 (1 #5# 1) 1 2 2 0 191) 
COMMENT                                            " N1: 7587 " 1))))) "MluI: 1 site") 
COMMENT                            1 5 "ACGCGT" "TGCGCA"))) 
COMMENT                    "Restriction/Methylation Map")))) 
COMMENT       (CGraphView 
COMMENT        (CWStyleSheet 
COMMENT         (CObjectList 
COMMENT          #163=(CWidgetStyle "RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #164=(CWidgetStyle "Signal Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #165=(CWidgetStyle "Molecule Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 16 
COMMENT                "@L bp" 0) 
COMMENT          #166=(CWidgetStyle "Molecule Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 "@N" 0) 
COMMENT          #167=(CWidgetStyle "Shape 3" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 255 0) 0 
COMMENT                0 0) 
COMMENT          #168=(CWidgetStyle "Shape 1" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 0 0 0) 
COMMENT          #169=(CWidgetStyle "Axis" 1 (LOGPEN 0 0 0) 2 1 (LOGBRUSH 0 12632256 0) 
COMMENT                0 0 0) 
COMMENT          #170=(CWidgetStyle "Line 2" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #171=(CWidgetStyle "RSite" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #172=(CWidgetStyle "Short Signal" 1 (LOGPEN 0 0 8388608) 10 0 0 0 0) 
COMMENT          #173=(CWidgetStyle "Uniq RSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 0 1 5 "@N (@S)" 0) 
COMMENT          #174=(CWidgetStyle "Vanilla" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 18 "Times New Roman") 0.8 0 
COMMENT                1 2 "?" 0) 
COMMENT          #175=(CWidgetStyle "Mark 1" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 2 7 48 2 2 "Windings") 0.7 0 1 2 "?" 
COMMENT                0) 
COMMENT          #176=(CWidgetStyle "Motif Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.64 0 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #177=(CWidgetStyle "Fragment Label 2" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 49 "Courier New") 1.05 0 1 48 
COMMENT                "@F bp (molecule @L bp)" 0) 
COMMENT          #178=(CWidgetStyle "Fragment Label 1" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.91 0 1 1 
COMMENT                "Fragment of @N" 0) 
COMMENT          #179=(CWidgetStyle "Shape 4" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 2 8388608 5) 0 0 0) 
COMMENT          #180=(CWidgetStyle "Shape 2" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 128 0) 0 
COMMENT                0 0) 
COMMENT          #181=(CWidgetStyle "Shape 0" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 0 0 0) 0 0 
COMMENT                0) #182=(CWidgetStyle "ORF" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #183=(CWidgetStyle "Line 4" 1 (LOGPEN 0 0 32768) 8 0 0 0 0) 
COMMENT          #184=(CWidgetStyle "Line 3" 1 (LOGPEN 0 0 16711680) 8 0 0 0 0) 
COMMENT          #185=(CWidgetStyle "Line 1" 1 (LOGPEN 0 0 16711680) 1 0 0 0 0) 
COMMENT          #186=(CWidgetStyle "Short Promoter" 1 (LOGPEN 0 0 128) 6 0 0 0 0) 
COMMENT          #187=(CWidgetStyle "Motif" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #188=(CWidgetStyle "Line 0" 1 (LOGPEN 0 0 0) 8 0 0 0 0) 
COMMENT          #189=(CWidgetStyle "Void" 0 0 0 0 0) 
COMMENT          #190=(CWidgetStyle "General Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                1 3 "@T @N " 0) 
COMMENT          #191=(CWidgetStyle "Position" 1 (LOGPEN 0 0 0) 1 0 0 0 0) 
COMMENT          #192=(CWidgetStyle "Annotation" 0 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 0.91 0 
COMMENT                0 0) 
COMMENT          #193=(CWidgetStyle "Position Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #194=(CWidgetStyle "Range" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #195=(CWidgetStyle "Range Label" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #196=(CWidgetStyle "ORF Label" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.888889 0 1 
COMMENT                65535 "@N" 0) 
COMMENT          #197=(CWidgetStyle "CDS Label" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.555556 255 1 1 
COMMENT                "@N" 0) 
COMMENT          #198=(CWidgetStyle "Shape 5" 1 (LOGPEN 0 0 3342336) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #199=(CWidgetStyle "CDS" 1 (LOGPEN 0 0 0) 1 1 (LOGBRUSH 2 39423 3) 0 0 
COMMENT                1 (LOGSHAPE 9 1 0.8 1.8 0)) 
COMMENT          #200=(CWidgetStyle "Label 2" 1 (LOGPEN 0 0 4227264) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.944444 8388608 
COMMENT                1 1 "@N" 0) 
COMMENT          #201=(CWidgetStyle "Label 3" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 700 255 0 0 0 3 2 1 34 "Arial") 0.833333 255 1 
COMMENT                5 "@N (@S)" 0) 
COMMENT          #202=(CWidgetStyle "Label 4" 1 (LOGPEN 0 0 8437824) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.722222 0 1 5 
COMMENT                "@N (@S)" 0) 
COMMENT          #203=(CWidgetStyle "Label 10" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #204=(CWidgetStyle "Promoter_E" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #205=(CWidgetStyle "Shape 10" 1 (LOGPEN 0 0 0) 3 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #206=(CWidgetStyle "Shape 13" 1 (LOGPEN 0 0 10040064) 6 1 
COMMENT                (LOGBRUSH 0 10040064 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #207=(CWidgetStyle "Label 20" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #208=(CWidgetStyle "Label 21" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #209=(CWidgetStyle "Shape 18" 1 (LOGPEN 0 0 0) 2 1 
COMMENT                (LOGBRUSH 0 8388736 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #210=(CWidgetStyle "Label 26" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #211=(CWidgetStyle "Shape GAP" 1 (LOGPEN 1 0 0) 3 1 
COMMENT                (LOGBRUSH 0 16777215 0) 0 0 0) 
COMMENT          #212=(CWidgetStyle "Label 27" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #213=(CWidgetStyle "Shape 20" 1 (LOGPEN 0 0 0) 1 1 
COMMENT                (LOGBRUSH 0 16750848 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #214=(CWidgetStyle "Label 28" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #215=(CWidgetStyle "Label 29" 1 (LOGPEN 0 0 8388608) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.63 8388608 1 1 
COMMENT                "@N" 0) 
COMMENT          #216=(CWidgetStyle "Mutation Label" 1 (LOGPEN 0 0 32896) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #217=(CWidgetStyle "Label 30" 1 (LOGPEN 0 0 0) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 1 0 0 3 2 1 34 "Arial") 0.666667 0 1 1 
COMMENT                "@N" 0) 
COMMENT          #218=(CWidgetStyle "MSite Label" 1 (LOGPEN 0 0 8421376) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 34 "Arial") 0.611111 255 1 -1 
COMMENT                "@N (@H%)" 0) 
COMMENT          #219=(CWidgetStyle "Label 34" 1 (LOGPEN 0 0 16724787) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 16711680 1 5 "@N (@S)" 0) 
COMMENT          #220=(CWidgetStyle "Label 45" 1 (LOGPEN 0 0 3381657) 1 0 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 3 2 1 18 "Times New Roman") 
COMMENT                0.611111 32896 1 5 "@N (@S)" 0) 
COMMENT          #221=(CWidgetStyle "Shape 22" 1 (LOGPEN 0 0 39321) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0) 
COMMENT          #222=(CWidgetStyle "Shape 23" 1 (LOGPEN 0 0 16724736) 1 1 
COMMENT                (LOGBRUSH 0 16777215 0) 1 
COMMENT                (LOGFONT 0 0 0 0 400 0 0 0 0 7 48 2 50 "Arial") 0.9 0 0 0)) 
COMMENT         0.164644 1.74233 0.164644 2.53336 
COMMENT         (15 (CShapeMapEntry 0 "Shape 5" 1 "Label 20") 9 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Position Label") 4 
COMMENT          (CShapeMapEntry 0 "Shape 3" 1 "Label 20") 0 
COMMENT          (CShapeMapEntry 8 "Shape 20" 1 "Label 21") 30 
COMMENT          (CShapeMapEntry 0 "Shape 18" 1 "Label 20") 29 
COMMENT          (CShapeMapEntry 0 "Promoter_E" 1 "Label 20") 26 
COMMENT          (CShapeMapEntry 0 "Line 0" 1 "Position Label") 25 
COMMENT          (CShapeMapEntry 0 "Line 4" 1 "Position Label") 22 
COMMENT          (CShapeMapEntry 1 "Shape 13" 1 "Label 20") 21 
COMMENT          (CShapeMapEntry 5 "Line 2" 1 "Label 21") 43 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 30") 33 
COMMENT          (CShapeMapEntry 0 "Shape 4" 1 "Label 20") 32 
COMMENT          (CShapeMapEntry 0 "Line 3" 1 "Label 21") 59 
COMMENT          (CShapeMapEntry 0 "Shape 10" 1 "Label 27") 62 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Mutation Label") 52 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 29") 50 
COMMENT          (CShapeMapEntry 0 "Line 2" 1 "Label 28") 69 
COMMENT          (CShapeMapEntry 0 "Motif" 1 "MSite Label") 70 
COMMENT          (CShapeMapEntry 0 "Shape 23" 1 "Label 34") 68 
COMMENT          (CShapeMapEntry 0 "Shape 22" 1 "Label 45") 86 
COMMENT          (CShapeMapEntry 8 "Shape 1" 1 "Label 26") 85 
COMMENT          (CShapeMapEntry 0 "Shape 1" 1 "Label 10")) 40.3368 40.3368 39 39 0.1 
COMMENT         -3261) 1 1 0 1 1 
COMMENT        (mapper: 23.4656 -16.9914 40.3368 40.3368 0.01 10 -1 9068 9068 1 0 0) 
COMMENT        #223=(CGroupWidget (CWidget 0 (0 0) 1 2 0 0 Nil 544367970 100) 
COMMENT              (CObjectList 
COMMENT               #224=(CGroupWidget (CWidget 1 (0 0) 1 2 0 0 Nil -326 100) 
COMMENT                     (CObjectList 
COMMENT                      #225=(CAxis 
COMMENT                            (CWideLine 
COMMENT                             (CWidget 0 (0 0) 1 2 0 0 #169# 73958396 0) 
COMMENT                             (LOGPEN 0 0 0) 2 (LOGBRUSH 0 12632256 0) 1 6.27488 
COMMENT                             6.27288 1 0.0214037) 0.0691794) 
COMMENT                      #226=(CLabel 
COMMENT                            (CWidget 1001 (0 0) 1 2 0 0 #166# 1953784163 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 63 24 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.91 0 "BiFC2" "@N" 1 0.0743738 2.28427 0 
COMMENT                            -10 3.94181 1.56185 Nil) 
COMMENT                      #227=(CLabel 
COMMENT                            (CWidget 1002 (0 0) 1 2 0 0 #165# 2082603850 100) 
COMMENT                            (LOGPEN 0 0 8388608) 1 
COMMENT                            (LOGFONT 73 27 0 0 400 0 0 0 0 3 2 1 49 
COMMENT                             "Courier New") 1.74233 1.05 0 "9068 bp" "@L bp" 16 
COMMENT                            0.0743738 0.294024 0 -10.8 4.78471 1.80976 Nil)) 
COMMENT                     (CObjectList)) 
COMMENT               #228=(CGroupWidget (CWidget 10 (6 0) 1 2 0 0 Nil -116 100) 
COMMENT                     (CObjectList 
COMMENT                      #229=(CGroupWidget 
COMMENT                            (CWidget 4 (7 4 0) 1 2 0 0 Nil -186 100) 
COMMENT                            (CObjectList 
COMMENT                             #230=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #10# 0) 1 2 0 0 #167# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    3.39532 3.60494 1 0.082322) 0.8 1.8 0) 
COMMENT                             #231=(CLabel (CWidget 0 (0 0) 1 2 0 0 #207# 1 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "ccdB" "@N" 1 
COMMENT                                   5.88276 -12.4442 0 -10 2.15684 1.16519 #230#) 
COMMENT                             #232=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #11# 0) 1 2 0 0 #167# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 2.6046 
COMMENT                                    3.15458 1 0.082322) 0.8 1.8 0) 
COMMENT                             #233=(CLabel (CWidget 0 (0 0) 1 2 0 0 #207# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "Tn5 neomycin resistance" "@N" 1 -9.09884 
COMMENT                                   -13.2623 0 -10 10.09 1.16519 #232#) 
COMMENT                             #234=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #15# 0) 1 2 0 0 #167# 861 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    1.02593 1.38497 1 0.082322) 0.8 1.8 0) 
COMMENT                             #235=(CLabel (CWidget 0 (0 0) 1 2 0 0 #207# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "yEmVenus fragment" "@N" 1 -16.7029 5.48184 0 
COMMENT                                   -10 8.20591 1.16519 #234#) 
COMMENT                             #236=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #18# 0) 1 2 0 0 #167# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 255 0) 1 
COMMENT                                    4.68483 5.46102 1 0.082322) 0.8 1.8 0) 
COMMENT                             #237=(CLabel (CWidget 0 (0 0) 1 2 0 0 #207# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "CmLEU2" "@N" 1 
COMMENT                                   14.7031 5.22699 0 -10 3.3964 1.16519 #236#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #238=(CGroupWidget 
COMMENT                            (CWidget 21 (7 21 0) 1 2 0 0 Nil -748 100) 
COMMENT                            (CObjectList 
COMMENT                             #239=(CLine 
COMMENT                                   (CWidget 0 (3 #9# 0) 1 2 0 0 #170# 878 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.835356 3.61116 
COMMENT                                   4.38459) 
COMMENT                             #240=(CLabel (CWidget 0 (0 0) 1 2 0 0 #208# 49 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "integration sequence" "@N" 1 12.9475 -9.5367 
COMMENT                                   0 -10 8.45382 1.16519 #239#) 
COMMENT                             #241=(CScratch 
COMMENT                                   (CWidget 0 (3 #13# 0) 1 2 0 0 #170# 580 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 1 1.42855 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #242=(CLabel (CWidget 0 (0 0) 1 2 0 0 #208# 0 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "start codon" 
COMMENT                                   "@N" 1 -15.7876 0.421451 0 -10 4.63597 
COMMENT                                   1.16519 #241#) 
COMMENT                             #243=(CScratch 
COMMENT                                   (CWidget 0 (3 #14# 0) 1 2 0 0 #170# 461 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 1 1.40988 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #244=(CLabel (CWidget 0 (0 0) 1 2 0 0 #208# 37 100) 
COMMENT                                   (LOGPEN 0 0 8388608) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "GGGGSGGGGS-linker" "@N" 1 -17.2517 2.98883 0 
COMMENT                                   -10 8.94964 1.16519 #243#)) (CObjectList)) 
COMMENT                      #245=(CGroupWidget 
COMMENT                            (CWidget 29 (7 29 0) 1 2 0 0 Nil -116 100) 
COMMENT                            (CObjectList 
COMMENT                             #246=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #12# 0) 1 2 0 0 #204# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 0 8388736 0) 
COMMENT                                    0.998345 1.4327 2.42889 1 0.082322) 0.8 1.8 
COMMENT                                   0) 
COMMENT                             #247=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #207# 780683472 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "CaMET3 promoter" "@N" 1 -16.0794 -5.57407 0 
COMMENT                                   -10 7.11509 1.16519 #246#)) (CObjectList)) 
COMMENT                      #248=(CGroupWidget 
COMMENT                            (CWidget 30 (7 30 0) 1 2 0 0 Nil -1100 100) 
COMMENT                            (CObjectList 
COMMENT                             #249=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #6# 0) 1 2 0 0 #209# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 2 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    6.15696 6.21507 1 0.082322) 0.8 1.8 0) 
COMMENT                             #250=(CLabel (CWidget 0 (0 0) 1 2 0 0 #207# 9 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "lac promoter" 
COMMENT                                   "@N" 1 3.96888 14.6824 0 -10 5.05742 1.16519 
COMMENT                                   #249#) 
COMMENT                             #251=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #7# 0) 1 2 0 0 #186# 0 100) 
COMMENT                                    (LOGPEN 0 4 128) 6 1.16464 6.02819 6.11546) 
COMMENT                                   0.4 0.082322 1.08464 1.8 1.8 0) 
COMMENT                             #252=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #207# 1900 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "SP6 promoter" 
COMMENT                                   "@N" 1 5.38183 13.0392 0 -10 5.47887 1.16519 
COMMENT                                   #251#) 
COMMENT                             #253=(CPromoterArrow 
COMMENT                                   (CLine 
COMMENT                                    (CWidget 0 (3 #8# 0) 1 2 0 0 #186# 0 100) 
COMMENT                                    (LOGPEN 0 4 128) 6 0.835356 4.48351 4.57078) 
COMMENT                                   0.4 0.082322 0.915356 1.8 1.8 1) 
COMMENT                             #254=(CLabel (CWidget 0 (0 0) 1 2 0 0 #207# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "T7 promoter" 
COMMENT                                   "@N" 1 14.3823 -3.08106 0 -10 4.88388 1.16519 
COMMENT                                   #253#) 
COMMENT                             #255=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #19# 0) 1 2 0 0 #209# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 2 (LOGBRUSH 0 8388736 0) 1 
COMMENT                                    2.48838 2.55479 1 0.082322) 0.8 1.8 0) 
COMMENT                             #256=(CLabel (CWidget 0 (0 0) 1 2 0 0 #207# 1 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "amp promoter" 
COMMENT                                   "@N" 1 -10.1719 -11.0242 0 -10 5.55324 
COMMENT                                   1.16519 #255#)) (CObjectList)) 
COMMENT                      #257=(CGroupWidget 
COMMENT                            (CWidget 33 (7 33 0) 1 2 0 0 Nil -678 100) 
COMMENT                            (CObjectList 
COMMENT                             #258=(CWideArrow 
COMMENT                                   (CWideLine 
COMMENT                                    (CWidget 0 (3 #17# 0) 1 2 0 0 #179# 0 100) 
COMMENT                                    (LOGPEN 0 0 0) 1 (LOGBRUSH 2 8388608 5) 1 
COMMENT                                    0.00484256 0.283635 1 0.082322) 0.8 1.8 0) 
COMMENT                             #259=(CLabel (CWidget 0 (0 0) 1 2 0 0 #207# 0 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 "pMB1 ori" "@N" 
COMMENT                                   1 -4.69274 13.9386 0 -10 3.56994 1.16519 
COMMENT                                   #258#)) (CObjectList)) 
COMMENT                      #260=(CGroupWidget 
COMMENT                            (CWidget 43 (7 43 0) 1 2 0 0 Nil -889 100) 
COMMENT                            (CObjectList 
COMMENT                             #261=(CLine 
COMMENT                                   (CWidget 0 (3 #16# 0) 1 2 0 0 #170# 878 100) 
COMMENT                                   (LOGPEN 0 6 16711680) 8 0.835356 0.8177 
COMMENT                                   1.00656) 
COMMENT                             #262=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #217# 257 100) 
COMMENT                                   (LOGPEN 0 0 0) 1 
COMMENT                                   (LOGFONT 46 17 0 0 400 0 1 0 0 3 2 1 34 
COMMENT                                    "Arial") 1.74233 0.666667 0 
COMMENT                                   "ACT1 terminator" "@N" 1 -13.2635 9.56844 0 
COMMENT                                   -10 6.54489 1.16519 #261#)) (CObjectList))) 
COMMENT                     (CObjectList)) 
COMMENT               #263=(CGroupWidget (CWidget 11 (8 0) 1 2 0 0 Nil -608 100) 
COMMENT                     (CObjectList 
COMMENT                      #264=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #0# 0) 1 2 0 0 Nil -1029 100) 
COMMENT                            (CObjectList 
COMMENT                             #265=(CScratch 
COMMENT                                   (CWidget 1 (1 #0# 1) 1 2 0 0 #222# 580 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 1.38843 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #266=(CLabel (CWidget 0 (0 0) 1 2 0 0 #219# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Nhe}I (7062)" "@N (@S)" 5 -15.1019 
COMMENT                                   4.23534 0 -10 4.16493 0.99165 #265#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #267=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #1# 0) 1 2 0 0 Nil 1212 100) 
COMMENT                            (CObjectList 
COMMENT                             #268=(CScratch 
COMMENT                                   (CWidget 1 (1 #1# 1) 1 2 0 0 #222# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 4.03108 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #269=(CLabel (CWidget 0 (0 0) 1 2 0 0 #219# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Not}I (3242)" "@N (@S)" 5 11.7326 
COMMENT                                   -8.21582 0 -10 4.09056 0.99165 #268#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #270=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #2# 0) 1 2 0 0 Nil -467 100) 
COMMENT                            (CObjectList 
COMMENT                             #271=(CScratch 
COMMENT                                   (CWidget 1 (1 #2# 1) 1 2 0 0 #222# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 1.0197 1.9 0.082322 
COMMENT                                   1) 
COMMENT                             #272=(CLabel (CWidget 0 (0 0) 1 2 0 0 #219# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Sca}I (7595)" "@N (@S)" 5 -13.5817 
COMMENT                                   8.07402 0 -10 4.01618 0.99165 #271#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #273=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #3# 0) 1 2 0 0 Nil -1170 100) 
COMMENT                            (CObjectList 
COMMENT                             #274=(CScratch 
COMMENT                                   (CWidget 1 (1 #3# 1) 1 2 0 0 #222# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 1.41887 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #275=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #219# 780681412 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Xma}I (7018)" "@N (@S)" 5 -15.6205 
COMMENT                                   1.74233 0 -10 4.33847 0.99165 #274#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #276=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #4# 0) 1 2 0 0 Nil -467 100) 
COMMENT                            (CObjectList 
COMMENT                             #277=(CScratch 
COMMENT                                   (CWidget 1 (1 #4# 1) 1 2 0 0 #222# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 1.43201 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #278=(CLabel (CWidget 0 (0 0) 1 2 0 0 #219# 1 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Bsp}EI (6999)" "@N (@S)" 5 -15.4463 
COMMENT                                   -0.924218 0 -10 4.58638 0.99165 #277#)) 
COMMENT                            (CObjectList)) 
COMMENT                      #279=(CGroupWidget 
COMMENT                            (CWidget 1 (10 #5# 0) 1 2 0 0 Nil -1170 100) 
COMMENT                            (CObjectList 
COMMENT                             #280=(CScratch 
COMMENT                                   (CWidget 1 (1 #5# 1) 1 2 0 0 #222# 0 100) 
COMMENT                                   (LOGPEN 0 0 16724736) 1 1 1.02524 1.9 
COMMENT                                   0.082322 1) 
COMMENT                             #281=(CLabel 
COMMENT                                   (CWidget 0 (0 0) 1 2 0 0 #219# 780684924 100) 
COMMENT                                   (LOGPEN 0 0 16724787) 1 
COMMENT                                   (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                                    "Times New Roman") 1.74233 0.611111 16711680 
COMMENT                                   "{\\i Mlu}I (7587)" "@N (@S)" 5 -14.2589 
COMMENT                                   6.72835 0 -10 4.16493 0.99165 #280#)) 
COMMENT                            (CObjectList))) (CObjectList)) 
COMMENT               #282=(CGroupWidget (CWidget 14 (16 0) 1 2 0 0 Nil 1169 100) 
COMMENT                     (CObjectList 
COMMENT                      #283=(CLabel 
COMMENT                            (CWidget 0 (15 #20# 0) 1 2 0 0 #192# 1936942450 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 63 24 0 0 400 0 0 0 0 3 2 1 18 
COMMENT                             "Times New Roman") 1.74233 0.91 0 "LMBP 10465" 
COMMENT                            "LMBP 10465" 65535 -3.55271e-015 -1.7106 0 -10 
COMMENT                            7.51175 1.56185 Nil) 
COMMENT                      #284=(CLabel 
COMMENT                            (CWidget 0 (15 #21# 0) 1 2 0 0 #192# 1936942450 100) 
COMMENT                            (LOGPEN 0 0 0) 1 
COMMENT                            (LOGFONT 42 16 0 0 400 0 0 0 0 3 2 1 34 "Arial") 
COMMENT                            1.74233 0.611111 0 "Last modified: 24-NOV-2017" 
COMMENT                            "Last modified: 24-NOV-2017" 65535 0.148748 -3.32203 
COMMENT                            0 -10 11.1809 1.06602 Nil)) (CObjectList)) 
COMMENT               #285=(CGroupWidget (CWidget 12 (0 0) 1 2 0 0 Nil -726 100) 
COMMENT                     (CObjectList) (CObjectList))) (CObjectList))) 
COMMENT       (CSeqView 10 6 (CObjectList) (CObList) 1 (CObList)) (CObList) 53477108 
COMMENT       (CStringList) 936 46968240 (CObList)))
FEATURES             Location/Qualifiers
     source          9059..9058
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /vntifkey="98"
     promoter        85..168
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        229..245
                     /vntifkey="30"
                     /label=SP6\promoter
     gene            311..2493
                     /vntifkey="60"
                     /note="CmLEU2 CmLEU2:   C. maltosa LEU2 gene. Complements  leu2-Delta/leu2-Delta deletion of C. albicans"
     promoter        complement(2588..2604)
                     /vntifkey="30"
                     /label=T7\promoter
     misc_feature    2731..3848
                     /vntifkey="21"
                     /label=integration\sequence
     CDS             3858..4160
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /vntifkey="4"
                     /label=ccdB
     CDS             4509..5303
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     promoter        5558..6997
                     /vntifkey="29"
                     /label=CaMET3\promoter
     misc_feature    7004..7006
                     /vntifkey="21"
                     /label=start\codon
                     /note="START CODON"
     misc_feature    7031..7060
                     /vntifkey="21"
                     /label=GGGGSGGGGS-linker
     CDS             7067..7585
                     /codon_start=1
                     /vntifkey="4"
                     /label=yEmVenus\fragment
                     /note="yEmVenus aa1-172"
     terminator      7614..7886
                     /vntifkey="43"
                     /label=ACT1\terminator
     rep_origin      8659..9061
                     /direction=RIGHT
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             1175..2296
                     /vntifkey="4"
                     /label=CmLEU2
                     /note="Candida maltosa LEU2"
     promoter        5376..5471
                     /vntifkey="30"
                     /label=amp\promoter
BASE COUNT     2617 a      1819 c      1938 g      2694 t 
ORIGIN
        1 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 
       61 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 
      121 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg 
      181 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat ttaggtgaca 
      241 ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca ctagtaacgg 
      301 ccgccagtgt gctggaattc gcccttggat ccaatcatca ctggtggggg tcaagaaaga 
      361 ggtttgagat caggtacttt ggcaccacca ttagtagctg ggtttggtga agctgcaaga 
      421 ttaatgaaac aagaatctgc atttgacaag aaacatattg agaggttatc tactaaattg 
      481 aagaacggtt tgttatccat tccatctact cagtttaacg gttgtaataa cccaacatat 
      541 caatacccag gttgtgtgaa tgtttccttt gcatacattg aaggggaatc tttgttaatg 
      601 gcattgaaag atattgcatt gagttctggt tctgcatgta cctctgcatc tttggaacca 
      661 tcgtatgttt tacatgcttt gggtgccgat gatgccttgg ctcattcttc cattagattc 
      721 ggtattggta gattcaccac tgaagcagaa gttgactatg ttattcaagc aattaatgaa 
      781 agagttgatt tcttgagaaa gatgtctcca ttatgggaaa tggttcaaga aggtattgac 
      841 ttgaactcta tcgaatggag tggacattaa ggttttttgt ttttttgtaa tttgtgttgt 
      901 tatgtgtaat tatcgtgtat agagtaattt cggaaattgt actttcgttc atgtatctga 
      961 accagatgaa taatacgttc gtattgttga agaatgacta actcctgtaa cggcgtgcgt 
     1021 gggttccgca gccgggttca attttttttt tcttgttcac tataaaaata tataataaac 
     1081 gaaaaaaatc cgagggactc aggctatgaa atttttcact caagaatttt ttttccttca 
     1141 tccttaattc ctttattcac tacccaaacc cacaatgtca gttaaaacta aaactattac 
     1201 aattttacca ggtgatcacg tcggtaccga aattgtcaat gaagctatca aagttttaga 
     1261 agcaattgaa gctgccactc cttaccaaaa aatccatttc gatttcaaac atcatttaat 
     1321 cggtggtgct gccattgatg ccactggtgt cccattgcca gatgacgcac ttgaaagtgc 
     1381 caaaaaatct gatgccgtct tattgggtgc tgttggtgga ccaaaatggg ggactggcgc 
     1441 cgttcgtcca gaacaaggtt tattaaagat tcgtaaagaa ttgaaccttt atgctaacat 
     1501 cagaccatgt aactttgcca gtgactcctt attggaatta tccccattga gaccagaagt 
     1561 cgttaaaggt acaaacttga tcattgttcg tgaattagtt ggtggtattt atttcggtga 
     1621 tcgtgaagaa caagaagaaa gtgaagataa acaaaccgca tgggatactg aaaaatacac 
     1681 cgtcgatgaa gtcaccagaa tcacccgtat ggctgccttc atggctttac aacataaccc 
     1741 accattacca atctggtcat tggataaggc taatgtttta gcttcttcta gattatggag 
     1801 aagaactgtc gataaagtga tttctgaaga gttcccaact ttgtctgttc aacatcaatt 
     1861 gattgactcc gccgccatga ttttaattca aaacccaacc aaattgaatg gtataatcat 
     1921 cacttctaac atgtttggtg atatcatttc cgatgaagct tcagttattc caggttcctt 
     1981 gggtttatta ccatctgcat ctttggcttc attgccagat accaacaccg cttttggtct 
     2041 ttatgaacca tgtcacggtt ctgctcctga tttaccagcc aataaagtta acccaattgc 
     2101 tactatttta tctgctgctt ctatgttgag attatctttg gattgtgtga aagaagctga 
     2161 agctttagaa gaagccgtga aacaagtctt ggataaaggt atcagaactg cagatttaag 
     2221 aggtaccagt tcagctactg aagttggtga cgcgattgtc gaagctgtta ctaaaatctt 
     2281 aaaagaaaaa gcttaagtaa agtatacaca aaatttaacg aatattatta atacaaaatg 
     2341 aacaccacct aaatactttt ttttaaaaat gtaccgactc ttctaaaaat tgcgataatt 
     2401 caatatcgga aatttctctc accggtttac ttggatcttc ggaaataaat ctatttgaag 
     2461 taatggtagc ctcacttgat tctgattggc tctttctaga catgggtagg taagggcgaa 
     2521 ttctgcagat atccatcaca ctggcggccg gccgctcgag catgcatcta gagggcccaa 
     2581 ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac gtcgtgactg 
     2641 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg 
     2701 gcgtaatagc gaagaggccc gcaccgatcg accagatcaa aatgctagaa caaattacgt 
     2761 ctgcatcaac agtcatcttc cttatttgca taaatgctaa aatggcaaac ctgtaaaccg 
     2821 tttgaaaagc aaattgagat agttagctcg gttttttttt cacgtggcca taatccaaca 
     2881 aaaaaaagga atttaagggg ggagagaagg aggcttgaat ttgtaccaca gagcgagcaa 
     2941 gaaattaata atatacttgg cttagctgaa atgagaatga aaaagcaaat ttcaagtgaa 
     3001 acaccactac tagccaagta aattggccaa tttgctctcg tgctttaatt tcctaacaag 
     3061 cttttgagac cgaaaaaaat caaccaatga tgatggaaaa atacttccgg gagtcaattt 
     3121 atccataatt tcacgtcgtt ttttttttgc acatggcaaa tatattggaa tcctgagatt 
     3181 ttcttggagt cgtgtacaat aacaccaggc gggaacatgc cgttatgatc cagcatatgg 
     3241 cggccgccat atgtgttcaa atattgaaac cgtgaaacca gtttgataca gctgatattg 
     3301 tgtgctaaaa aaagatgagc accgaaagag tctttagttt attgccaagt aaagggtcaa 
     3361 agaataccta ttctttatga acacagactt gctctaatgg tgaattagtg tggagtaata 
     3421 gagaaggagg cgaaacaaaa ataattggtt aaacactcga atgtcacaca atcctaaata 
     3481 ggttaaattc agttaccgcc gtgatgcggg aacaaagccg agcggtaata tttttggatt 
     3541 ctatgttttt ttggtgtata ttgcacgact tggtgccaac aactggtgtt ccaacgcaca 
     3601 aaactaggtt caactggata ctgttttcta gaaattattt gcggcaattc atgattacgg 
     3661 tcctaaattt gaaataaaag tttccaatta tgtgtcttga caatttccat tgggttcaaa 
     3721 aagatctaga atgtatgtaa ttacgcaaca atttgcatgc aaagaaataa aaacaaacct 
     3781 aaaatccaac tgctgttgtt ctggtgatga tgtagtaaaa taagagcacg gccaagaaca 
     3841 aggtgattta cgtacggcag tttaaggttt acacctataa aagagagagc cgttatcgtc 
     3901 tgtttgtgga tgtacagagt gatattattg acacgccggg gcgacggatg gtgatccccc 
     3961 tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg gtggtgcata 
     4021 tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg gtctccgtta 
     4081 tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc 
     4141 tgatgttctg gggaatataa atgtcaggca tgagattatc aaaaaggatc ttcacctaga 
     4201 tccttttcac gtagaaagcc agtccgcaga aacggtgctg accccggatg aatgtcagct 
     4261 actgggctat ctggacaagg gaaaacgcaa gcgcaaagag aaagcaggta gcttgcagtg 
     4321 ggcttacatg gcgatagcta gactgggcgg ttttatggac agcaagcgaa ccggaattgc 
     4381 cagctggggc gccctctggt aaggttggga agccctgcaa agtaaactgg atggctttct 
     4441 cgccgccaag gatctgatgg cgcaggggat caagctctga tcaagagaca ggatgaggat 
     4501 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga 
     4561 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc 
     4621 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga 
     4681 atgaactgca agacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg 
     4741 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc 
     4801 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg 
     4861 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga 
     4921 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc 
     4981 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgagca 
     5041 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg 
     5101 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct 
     5161 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg 
     5221 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc 
     5281 gccttcttga cgagttcttc tgaattatta acgcttacaa tttcctgatg cggtattttc 
     5341 tccttacgca tctgtgcggt atttcacacc gcatacaggt ggcacttttc ggggaaatgt 
     5401 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag 
     5461 acaataaccc tgataaatgc ttcaataata gcacgtgagg agggccacca tggccaagtt 
     5521 gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcttc caattagagg ttgtggatgc 
     5581 aaaattgaaa aaaaaatgac tattggtagc gtgttctatt tttcttatgc gctgaaaaac 
     5641 tacgaacaat tgtctattcc aagcctgtgg ctttttttgg tgattcacaa tacataaatt 
     5701 ggcaaatacc tcttctatag actttaacct ttgatgaaat ggtatggata aaccactttt 
     5761 gtggtcaggg tatatatatt tttattccaa tctggccttt ataaattcaa atttccaagg 
     5821 ggactctgct aaaccttctg atataactat aactctaata atggctgtaa aaagtgtgaa 
     5881 aaaaagatga tattcacaaa aaagataaag gggatttcat attgggtatc tagatttaaa 
     5941 catcgtattt caacaatgaa actagaatca atacgaaatc cagggcacaa ctgtgttcat 
     6001 tcccttggga attatactga gtaatgggaa gaatgtgcaa aaaatacccg tgtcttatat 
     6061 aatttccatt tgtgtagggc ttaaaagtgg acaacttcag gttacgaaac cacgaaaatg 
     6121 tctaaacgtt tgatcaaatt cgtaactcat ggaaaatcac agcaacaaat taaggatgga 
     6181 tatatccggt acttacaaat aaaaacacta aagaagtata aattttgaac tgaaattcta 
     6241 tagaaaatct ttttttttgg gtaaaattat gcgattgtgg ctcatagtaa cgtaatcata 
     6301 cagttcaaca aacatcattt tgtcaaagga tttctaatcc tattcttttt ctacttggct 
     6361 tttgagtata tatatatata tatatacatc ctttgttcat catcatgttc gattgaattt 
     6421 ttttcttggt tgttattaga ttgtctactt gagtttttct tggtgcaaat tgtggtgttt 
     6481 ttctaacaaa tttttcaaca tttaaagtat ttctggaact tttctacaag gaacgacata 
     6541 gatccattaa tgcgcccctc taaaacaata cccataatgt cttcttaagt atgcatctgc 
     6601 ttataaatgt ttctggattt actttagtta gaatgtcgac acgacaatga actattgctg 
     6661 tagatcacgt gcgatcactg aattatgagt ttctttttat ttttcttttt gggctttttt 
     6721 ttttgcaatt ttttttttcc acctttgcca attgcgtagt cacatataat gtatacaatt 
     6781 tttgcaactc tgacactata attgatccaa taataaagta tataatagat catcaagtat 
     6841 acgtaatctc cccaaaaaaa aaattgcata aaataaaatc tataaacctt ttttagattt 
     6901 ttagttgttc cttttttctt tctattcttc aacttccaag tgttgtcact ttcttcctgt 
     6961 taactatatt taaaagtaaa taccctcccc agaaaactcc ggaatgagcc tagggacccg 
     7021 ggggcgcgcc ggaggtggag gttctggtgg aggtggttca gctagcatgt ctaaaggtga 
     7081 agaattattc actggtgttg tcccaatttt ggttgaatta gatggtgatg ttaatggtca 
     7141 caaattttct gtctccggtg aaggtgaagg tgatgctact tacggtaaat tgaccttaaa 
     7201 attgatttgt actactggta aattgccagt tccatggcca accttagtca ctactttagg 
     7261 ttatggtttg caatgttttg ctagataccc agatcatatg aaacaacatg actttttcaa 
     7321 gtctgccatg ccagaaggtt atgttcaaga aagaactatt tttttcaaag atgacggtaa 
     7381 ctacaagacc agagctgaag tcaagtttga aggtgatacc ttagttaata gaatcgaatt 
     7441 aaaaggtatt gattttaaag aagatggtaa cattttaggt cacaaattgg aatacaacta 
     7501 taactctcac aatgtttaca tcactgctga caaacaaaag aatggtatca aagctaactt 
     7561 caaaattaga cacaacattg aataaacgcg tagtacttaa ccgcgccgtc gacgagtgaa 
     7621 attctggaaa tctggaaatc tggttttgta ttcttgttat tcttcttttt gttattacat 
     7681 atataacttg ttactttttt aaaaaaatct ttgtttattt tataaatata taaaactaaa 
     7741 tttaagaaaa agagaaaaat gttttatttg agagattgaa attttacttg aatttagctt 
     7801 agcttttata aagtattatt atgtaaaaaa acaaaacaaa tatacattaa aaagttaaga 
     7861 ctataaaata gccacccaag gcatttctat atcttgttgt tgttgttttc atcttctgta 
     7921 tcagaggaac ttattttatt attttcgtca cgggtatttt ctcttgtttg atgattcatc 
     7981 ccattcattc catcataaaa ttctagaagg accacctttg attgtaaata gtaataatta 
     8041 ccacccttat ttaattattt atttaactta tttatttatt tattatacat atatacaaat 
     8101 ctaataaagt gaaaatctcc cccttcacac gtggccgagg agcaggactg acacgtgcta 
     8161 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 
     8221 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa 
     8281 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca 
     8341 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta 
     8401 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc 
     8461 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca 
     8521 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta 
     8581 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag 
     8641 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt 
     8701 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc 
     8761 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac 
     8821 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac 
     8881 gccagcaacg cggccttttt acggttcctg ggcttttgct ggccttttgc tcacatgttc 
     8941 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat 
     9001 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag 
     9061 cgcccaat 
//
