LOCUS       BiFC3                   9743 bp    DNA     circular SYN 24-NOV-2017
DEFINITION  LMBP 10466.
ACCESSION   .
VERSION     .
KEYWORDS    BiFC3.
SOURCE      synthetic DNA construct.
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Friday, Aug 4, 2017 from SnapGene 3.2.0
            http://www.snapgene.com
COMMENT     Accession number: LMBP 10466
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 24-NOV-2017
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12042029|
COMMENT     VNTDBDATE|-12042462|
COMMENT     LSOWNER|
COMMENT     VNTNAME|BiFC3|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|UNKNOWN|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
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COMMENT        #2=(CRSite (CStringList) "ScaI" "agtact" 4 0 1 8021 0 0 "") 
COMMENT        #3=(CRSite (CStringList) "XmaI" "cccggg" 2 0 1 7996 0 0 "") 
COMMENT        #4=(CRSite (CStringList) "BspEI" "tccgga" 2 0 1 7743 0 0 "") 
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COMMENT                          "Currently local object. Original author: UNKNOWN" 1) 
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COMMENT                          "Modification Date in the Original DB: 18-DEC-2015" 1) 
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COMMENT                          "Original Accession Number(s): <a href=\"ncbilink:dnarna/.\">.</a>" 
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COMMENT                          "Organizm(s): <a href=\"ncbilink:taxonomy/synthetic+DNA+construct\">synthetic DNA construct(synthetic DNA construct)</a>" 
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FEATURES             Location/Qualifiers
     source          9734..9733
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
                     /vntifkey="98"
     promoter        85..168
                     /vntifkey="30"
                     /label=lac\promoter
     promoter        229..245
                     /vntifkey="30"
                     /label=SP6\promoter
     gene            296..3498
                     /vntifkey="60"
                     /note="CdARG4"
     misc_feature    3596..4453
                     /vntifkey="21"
                     /label=integration\sequence
     CDS             4602..4904
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /vntifkey="4"
                     /label=ccdB
     CDS             5253..6047
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /vntifkey="4"
                     /label=Tn5\neomycin\resistance
     promoter        6302..7741
                     /vntifkey="29"
                     /label=CaMET3\promoter
     CDS             7748..7948
                     /codon_start=1
                     /vntifkey="4"
                     /label=yEmVenus\fragment
                     /note="yEmVenus aa178-238"
     misc_feature    7955..7984
                     /vntifkey="21"
                     /label=GGGGSGGGGS-linker
     misc_feature    8009..8011
                     /vntifkey="21"
                     /label=stop\codon
                     /note="STOP CODON"
     terminator      8052..8324
                     /vntifkey="43"
                     /label=ACT1\terminator
     rep_origin      9334..9736
                     /direction=RIGHT
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        6120..6215
                     /vntifkey="30"
                     /label=amp\promoter
     CDS             1321..2715
                     /vntifkey="4"
                     /label=CdARG4
BASE COUNT     2836 a      1883 c      1930 g      3094 t 
ORIGIN
        1 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt 
       61 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta 
      121 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg 
      181 ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat ttaggtgaca 
      241 ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca ctagtttctg 
      301 taccgcaaat gtatcgactt tttcgatggt gtgttattgt tgttttgttg cgaaatataa 
      361 tagcttctgc tttatatcct ttgtgttgat ccttcccctc ctttcatact tgtaaaatta 
      421 caattggtat tttcaaccaa tattaccaca cgtcatgcac taactcaaat gggcaaaaaa 
      481 aagagagata ggaattacgt cattttatta tgtttttacg tggactagaa ggttaagata 
      541 atttctggtt attttgttga taattgagtt ttgttcgacc tccaccaatc tttaaactct 
      601 cgttgagtat tcattttaat gattttttga aatcagaagg ggtgtttcta acccaggatt 
      661 gttttattga gaggttgaaa catcaatgtg ttagtttatt gatttgggtt ggtttaatag 
      721 aaaaatatca gtaggtgttc aataattgta gtttattttc ttggctaaaa ttgttgcgaa 
      781 aaattgacaa aataataaca aaaagtattt tagatgctaa aacataatag taggaatatc 
      841 tacctgaacc atttacattc aacaactggc aagaaccttc ctaataattt gcaagagtaa 
      901 tcacaattaa accaaaatca aagtttaccg tgtatatagt tggacagaaa gctgtcacat 
      961 tggtttaata catagttgaa tatgtatgca tgaaacaaca acaatacaat tacagctcat 
     1021 gatccttcaa ttaatttcaa cattcaaaca acataacacc ttgtgtaaat gaggttatga 
     1081 gtacatgtga acaacaaatc ttatgactaa actcgggtcg tgtacttttg acgattgagt 
     1141 catttttacc aattaggcaa ttaatttttg ctaaattttc attaaccgcg ctggccatcc 
     1201 caacaaacaa tataaaagct gacaacgaaa aaaaaaaaaa aaaaaaaaaa actccaattc 
     1261 tgaaaaattt ttcttcttct tcatactaaa caaaacaact ataaagataa tcaattaata 
     1321 atgtcacaac aacctagtga aaataaatta tggggtggtc gattcactgg cgccactgat 
     1381 ccattaatgg atttatacaa tgcttcatta ccttatgata aagttatgta tgatgcagat 
     1441 ttaactggta ctaaagtata tactcaagga ttgaacaaat taggattaat taccactgaa 
     1501 gaattgaatc tgattcatca aggtttagaa caaattagac aagaatggca tgataataaa 
     1561 tttattatta aagctggtga tgaagatatt catactgcta atgaacgtag attaggagaa 
     1621 atcattggga aaaacatttc tggtaaagtt catactggta gatccagaaa tgatcaagtt 
     1681 gctactgata tgagaatttt tgttcgtgaa tcattactta atttaagtaa gattttacat 
     1741 caattcatta cagcaattct tgaacgagct cataaagaaa ttgatgtatt aatgcctggt 
     1801 tatactcatt tacaaaaagc tcaacctatt agatgggctc attggttaag ttcatatgca 
     1861 acttatttca ctgaagatta taaacgttta caagaaatca ttactcgtgt taatcaatct 
     1921 ccattaggat ctggtgcttt agctggtcat ccctacggta ttgatcgtga atttttagct 
     1981 aaaggattag gatttgatgg tgttattggg aattctttaa ctgcagtttc tgatcgtgat 
     2041 tttgttgttg aaagtttatt ttggagtaca ttatttatga atcatatttc tcgattttca 
     2101 gaagatttaa ttatttattc tagtggtgaa tttggattta taaagttagc tgatgcttac 
     2161 tctactggtt catcattaat gccacaaaag aaaaatcctg attcattaga attattacgt 
     2221 gggaaaagtg gcagagtgtt tggtcagtta tctgggtttt taatgtcaat taaatccatt 
     2281 ccatcaacct ataataaaga tatgcaagaa gataaagaac cattatttga tgcattgact 
     2341 accgtagaac attcaatttt aattgctact ggtgtgattt ctactttact gattgataaa 
     2401 caaaatatgg aaaaagcttt aactatggat atgttagcta ctgatttagc agattattta 
     2461 gttagaaaag gtgttccatt tagagaaact catcatattt ctggtgaatg tgttagaaaa 
     2521 gctgaagaag aaaaattgtc gggaattgac caattatcat ttgaacaatt tcaacaaatt 
     2581 gattcacgtt ttgaaaaaga tgttatggaa acttttgatt ttgaagctag tgtggaaaga 
     2641 agagatgcta ttggtggtac tgctaagagc gctgttttaa aacagttaga aaatttgaaa 
     2701 tcaattttaa attaatttat aaataatcat atagtattca atgttttgtt ttgttttttt 
     2761 tttttttttt atggaaatgt acaaacagta tataatcaat taatagaaag ataccctgta 
     2821 ttccattgta tcgcctttgg gatgtctata ataccaactt tcaagaatga gaaattaaac 
     2881 aacaatttct ttcaccaatc aactcgtcta tattcaataa actcattctg gtcttttcaa 
     2941 atatttaatg ttatcctgat caaacttaca aatcaaaaat gtatcaaaat cattcattag 
     3001 ttcttcttct tttgttatct ttgtcactat atttctctcc atccagacca aaaatacttc 
     3061 cattgtgaag atcgtctgaa tcataatcag caactacctc ttccatttca gagaattcag 
     3121 atttttcaaa ataggtatca cgggccaagt acacacaaga caaacgatac gagtgctggg 
     3181 atatttcttt caatgtatca ctaaattcgt tgagatttaa agagagccct ttgactgctt 
     3241 tcattacttt cctcacatca tgagcatcca cattcaaatt tcctgtagcc ctttattctt 
     3301 agttttcttt tcttattctt ttcttcgtac attgaaatca caaatggtga tatcaaacga 
     3361 aggtcacact gacttatgtc taatacctcc aatgatgata ctggtaactt aaccaggtct 
     3421 actcttttca aacgatttct cattagtgtc aatttacgta ggttcttcaa cctttcaaac 
     3481 gatgcaaata gcttttgtaa cggccgccag tgtgctggaa ttgcccttaa gggcgaattc 
     3541 tgcagatatc catcacactg gcggccggcc gctcgagcat gcatctagag ggcccttgaa 
     3601 gaattggaag aagttggcag ttaaaaaaca atgggaaaga tttaatcttt tttttcttct 
     3661 ttcaaaaatt tttaaccgct gatgatatat taatctctct ccaccactct cgttaaacac 
     3721 cacaattatt aataataaga tatctctcct actaaatatt tgttagtaag tatattgttg 
     3781 attgattggt tgggttaaaa attttttttt tcataaagaa aataatgaaa gtgggaaaaa 
     3841 tttaaacccc acattacata ctcaacacaa cactacacac acgcatttaa caatcatcat 
     3901 cagttggaat atattttaca aacaattacg attgtttaat gaaaactatt tctgttatta 
     3961 ttatttaacc tttttttttt cttgttgggt tgaccctttt tctattaact atctaactac 
     4021 cacataacat ttgaagttga actattgaac cttaatttgc ggccgcaaat ttaatcttct 
     4081 ttcttcttcc accttttttt tatttttttg aaatgggggg tggtgggaac tttaggcgtg 
     4141 ttaaatttgt cacatctgtt tcacttgctt ctattcgtat aattttaatt gtcttgtgag 
     4201 atcaatcttg ttcttgctct tgttttttct tgctttgatt aactaccatt attattagta 
     4261 tgcaatgctg tttattacca tttcataact tatattataa ctcattgatt tcaatacctg 
     4321 ctcaacaatg agtcgttatt attactgtgc tctgtattct gtactgctgc acgtacgtta 
     4381 tgtccattga agacaagcaa agaaagacca acattcaatt gtatctgctt accagagttg 
     4441 tttacctctg ttaccaatat ttggaaatta attttttggt ggtggacgtt tcttcctaaa 
     4501 acacctagat gtgctagtat aattgcacaa ccagcacgca cactgcgtct ggccggtcct 
     4561 ggactacatt ttgtctctaa cattcgtagc tgcacgtacg gcagtttaag gtttacacct 
     4621 ataaaagaga gagccgttat cgtctgtttg tggatgtaca gagtgatatt attgacacgc 
     4681 cggggcgacg gatggtgatc cccctggcca gtgcacgtct gctgtcagat aaagtctccc 
     4741 gtgaacttta cccggtggtg catatcgggg atgaaagctg gcgcatgatg accaccgata 
     4801 tggccagtgt gccggtctcc gttatcgggg aagaagtggc tgatctcagc caccgcgaaa 
     4861 atgacatcaa aaacgccatt aacctgatgt tctggggaat ataaatgtca ggcatgagat 
     4921 tatcaaaaag gatcttcacc tagatccttt tcacgtagaa agccagtccg cagaaacggt 
     4981 gctgaccccg gatgaatgtc agctactggg ctatctggac aagggaaaac gcaagcgcaa 
     5041 agagaaagca ggtagcttgc agtgggctta catggcgata gctagactgg gcggttttat 
     5101 ggacagcaag cgaaccggaa ttgccagctg gggcgccctc tggtaaggtt gggaagccct 
     5161 gcaaagtaaa ctggatggct ttctcgccgc caaggatctg atggcgcagg ggatcaagct 
     5221 ctgatcaaga gacaggatga ggatcgtttc gcatgattga acaagatgga ttgcacgcag 
     5281 gttctccggc cgcttgggtg gagaggctat tcggctatga ctgggcacaa cagacaatcg 
     5341 gctgctctga tgccgccgtg ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca 
     5401 agaccgacct gtccggtgcc ctgaatgaac tgcaagacga ggcagcgcgg ctatcgtggc 
     5461 tggccacgac gggcgttcct tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg 
     5521 actggctgct attgggcgaa gtgccggggc aggatctcct gtcatctcac cttgctcctg 
     5581 ccgagaaagt atccatcatg gctgatgcaa tgcggcggct gcatacgctt gatccggcta 
     5641 cctgcccatt cgaccaccaa gcgaaacatc gcatcgagcg agcacgtact cggatggaag 
     5701 ccggtcttgt cgatcaggat gatctggacg aagagcatca ggggctcgcg ccagccgaac 
     5761 tgttcgccag gctcaaggcg agcatgcccg acggcgagga tctcgtcgtg acccatggcg 
     5821 atgcctgctt gccgaatatc atggtggaaa atggccgctt ttctggattc atcgactgtg 
     5881 gccggctggg tgtggcggac cgctatcagg acatagcgtt ggctacccgt gatattgctg 
     5941 aagagcttgg cggcgaatgg gctgaccgct tcctcgtgct ttacggtatc gccgctcccg 
     6001 attcgcagcg catcgccttc tatcgccttc ttgacgagtt cttctgaatt attaacgctt 
     6061 acaatttcct gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac 
     6121 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca 
     6181 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatagcacgt 
     6241 gaggagggcc accatggcca agttgaccag tgccgttccg gtgctcaccg cgcgcgacgt 
     6301 cttccaatta gaggttgtgg atgcaaaatt gaaaaaaaaa tgactattgg tagcgtgttc 
     6361 tatttttctt atgcgctgaa aaactacgaa caattgtcta ttccaagcct gtggcttttt 
     6421 ttggtgattc acaatacata aattggcaaa tacctcttct atagacttta acctttgatg 
     6481 aaatggtatg gataaaccac ttttgtggtc agggtatata tatttttatt ccaatctggc 
     6541 ctttataaat tcaaatttcc aaggggactc tgctaaacct tctgatataa ctataactct 
     6601 aataatggct gtaaaaagtg tgaaaaaaag atgatattca caaaaaagat aaaggggatt 
     6661 tcatattggg tatctagatt taaacatcgt atttcaacaa tgaaactaga atcaatacga 
     6721 aatccagggc acaactgtgt tcattccctt gggaattata ctgagtaatg ggaagaatgt 
     6781 gcaaaaaata cccgtgtctt atataatttc catttgtgta gggcttaaaa gtggacaact 
     6841 tcaggttacg aaaccacgaa aatgtctaaa cgtttgatca aattcgtaac tcatggaaaa 
     6901 tcacagcaac aaattaagga tggatatatc cggtacttac aaataaaaac actaaagaag 
     6961 tataaatttt gaactgaaat tctatagaaa atcttttttt ttgggtaaaa ttatgcgatt 
     7021 gtggctcata gtaacgtaat catacagttc aacaaacatc attttgtcaa aggatttcta 
     7081 atcctattct ttttctactt ggcttttgag tatatatata tatatatata catcctttgt 
     7141 tcatcatcat gttcgattga atttttttct tggttgttat tagattgtct acttgagttt 
     7201 ttcttggtgc aaattgtggt gtttttctaa caaatttttc aacatttaaa gtatttctgg 
     7261 aacttttcta caaggaacga catagatcca ttaatgcgcc cctctaaaac aatacccata 
     7321 atgtcttctt aagtatgcat ctgcttataa atgtttctgg atttacttta gttagaatgt 
     7381 cgacacgaca atgaactatt gctgtagatc acgtgcgatc actgaattat gagtttcttt 
     7441 ttatttttct ttttgggctt ttttttttgc aatttttttt ttccaccttt gccaattgcg 
     7501 tagtcacata taatgtatac aatttttgca actctgacac tataattgat ccaataataa 
     7561 agtatataat agatcatcaa gtatacgtaa tctccccaaa aaaaaaattg cataaaataa 
     7621 aatctataaa ccttttttag atttttagtt gttccttttt tctttctatt cttcaacttc 
     7681 caagtgttgt cactttcttc ctgttaacta tatttaaaag taaataccct ccccagaaaa 
     7741 ctccggaatg gatggtggtg ttcaattagc tgaccattat caacaaaata ctccaattgg 
     7801 tgatggtcca gtcttgttac cagacaacca ttacttatcc tatcaatcta aattatccaa 
     7861 agatccaaac gaaaagagag accacatggt cttgttagaa tttgttactg ctgctggtat 
     7921 tacccatggt atggatgaat tgtacaaagc tagcggaggt ggaggttctg gtggaggtgg 
     7981 ttcaagccta gggacccggg ggcgcgccta aacgcgtagt acttaagatc ctgaagacgg 
     8041 gatcgttcga agagtgaaat tctggaaatc tggaaatctg gttttgtatt cttgttattc 
     8101 ttctttttgt tattacatat ataacttgtt acttttttaa aaaaatcttt gtttatttta 
     8161 taaatatata aaactaaatt taagaaaaag agaaaaatgt tttatttgag agattgaaat 
     8221 tttacttgaa tttagcttag cttttataaa gtattattat gtaaaaaaac aaaacaaata 
     8281 tacattaaaa agttaagact ataaaatagc cacccaaggc atttctatat cttgttgttg 
     8341 ttgttttcat cttctgtatc agaggaactt attttattat tttcgtcacg ggtattttct 
     8401 cttgtttgat gattcatccc attcattcca tcataaaatt ctagaaggac cacctttgat 
     8461 tgtaaatagt aataattacc acccttattt aattatttat ttaacttatt tatttattta 
     8521 ttatacatat atacaaatct aataaagtga aaatctcccc cttcacccgg tgtggtccgg 
     8581 gacgacgtga ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg 
     8641 gcctgggtgt gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc 
     8701 acgaacttcc gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg 
     8761 cgggagttcg ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag 
     8821 gactgacacg tgctaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt 
     8881 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc 
     8941 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg 
     9001 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact 
     9061 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg 
     9121 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg 
     9181 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac 
     9241 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca 
     9301 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga 
     9361 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc 
     9421 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct 
     9481 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg 
     9541 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctgggctt ttgctggcct 
     9601 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc 
     9661 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc 
     9721 gaggaagcgg aagagcgccc aat 
//
