LOCUS       pNIM1A-tTA              9273 bp    DNA     circular     14-JUL-2020
DEFINITION  LMBP 10509.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 10509
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 14-JUL-2020
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12122609|
COMMENT     VNTDBDATE|-13429032|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pNIM1A-tTA|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|
COMMENT     VNTREPLTYPE|Plasmid
COMMENT     VNTREPLTYPE|Phage
COMMENT     VNTEXTCHREPL|Bacteria
COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pNIM1A-tTA" 0 9273 
COMMENT       (CDBMol 0 0 1 1 1 0 0 0 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 1 0 0 3 75) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList "BamHI" "HindIII" "NdeI" "PvuI") 
COMMENT        (CStringList "atg" "gtg") (CStringList "taa" "tga" "tag") (CObList) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 0 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 862 853 1 9273 0 0 0 0 0 0 0 0) (CStringList) 
COMMENT       (CStringList) (CObList) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (CObList #0=(CRSite (CStringList) "BamHI" "ggatcc" 2 0 1 1393 0 0 "") 
COMMENT        #1=(CRSite (CStringList) "HindIII" "aagctt" 2 0 3 1287 0 5997 0 6748 0 0 
COMMENT            "") 
COMMENT        #2=(CRSite (CStringList) "NdeI" "catatg" 3 0 4 2113 0 2894 0 3422 0 5503 
COMMENT            0 0 "") 
COMMENT        #3=(CRSite (CStringList) "PvuI" "cgatcg" 5 0 2 502 0 8729 0 0 "")) 
COMMENT       (CObList 
COMMENT        #4=(CFSignal (CObList) "T7 promoter" 30 0 0 627 643 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "627..643") 
COMMENT        #5=(CFSignal (CObList) "T3 promoter" 30 0 1 7087 7103 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(7087..7103)") 
COMMENT        #6=(CFSignal (CObList) "lac promoter" 30 0 1 7167 7250 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(7167..7250)") 
COMMENT        #7=(CFSignal (CObList) "pMB1 ori" 33 0 1 7342 7744 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(7342..7744)") 
COMMENT        #8=(CFSignal (CObList) "ampicillin resistance" 4 0 1 8285 9145 0 
COMMENT            (CStringList) (CStringList) 1 1 1 1 "complement(8285..9145)") 
COMMENT        #9=(CFSignal (CObList) "f1 ori" 33 0 1 4 459 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(4..459)") 
COMMENT        #10=(CFSignal (CObList) "CaADH1 promoter" 29 0 0 668 1532 0 
COMMENT             (CStringList) (CStringList "/regulatory_class=\"promoter\"") 1 1 1 
COMMENT             1 "668..1532") 
COMMENT        #11=(CFSignal (CObList) "CatTA" 4 0 0 1533 2516 0 (CStringList) 
COMMENT             (CStringList "/gene=\"catTA\"" "/codon_start=1" "/transl_table=12" 
COMMENT              "/product=\"tetracycline regulated transactivator\"" 
COMMENT              "/protein_id=\"ART66047.1\"") 1 1 1 1 "1533..2516") 
COMMENT        #12=(CFSignal (CObList) "CaQCR7 bidirectional terminator" 43 0 0 2540 
COMMENT             2861 0 (CStringList) 
COMMENT             (CStringList "/regulatory_class=\"terminator\"") 1 1 1 1 
COMMENT             "2537..2891") 
COMMENT        #13=(CFSignal (CObList #14=(CLocSeg 0 562) #15=(CLocSeg 1217 9)) 
COMMENT             "nourseothricin resistance" 4 0 1 2898 4124 0 (CStringList) 
COMMENT             (CStringList "/gene=\"CaSAT1\"" "/codon_start=1" "/transl_table=12" 
COMMENT              "/product=\"nourseothricin resistance marker\"" 
COMMENT              "/protein_id=\"ART66048.1\"") 1 1 1 1 
COMMENT             "complement(2898..3460,4115..4124)") 
COMMENT        #16=(CFSignal (CObList) "CaACT1 promoter" 29 0 1 4125 4622 0 
COMMENT             (CStringList) (CStringList "/regulatory_class=\"promoter\"") 1 1 1 
COMMENT             1 "complement(4125..4622)") 
COMMENT        #17=(CFSignal (CObList) "CaACT1 terminator" 43 0 1 4744 5011 0 
COMMENT             (CStringList) (CStringList "/regulatory_class=\"terminator\"") 1 1 
COMMENT             1 1 "complement(4744..5011)") 
COMMENT        #18=(CFSignal (CObList) "CaGFP" 4 0 1 5018 5734 0 (CStringList) 
COMMENT             (CStringList "/gene=\"GFP\"" "/codon_start=1" "/transl_table=12" 
COMMENT              "/product=\"green fluorescent protein\"" 
COMMENT              "/protein_id=\"ART66049.1\"") 1 1 1 1 "complement(5018..5734)") 
COMMENT        #19=(CFSignal (CObList) "CaOPS4T promoter" 29 0 1 5745 6399 0 
COMMENT             (CStringList) (CStringList "/regulatory_class=\"promoter\"") 1 1 1 
COMMENT             1 "complement(5745..6110)") 
COMMENT        #20=(CFSignal (CObList) "3' CaADH1 integration site" 21 0 0 6414 7060 0 
COMMENT             (CStringList) (CStringList) 1 1 1 1 "6414..7060") 
COMMENT        #21=(CFSignal (CObList) "tetO" 21 0 1 6255 6273 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #22=(CFSignal (CObList) "tetO" 21 0 1 6171 6189 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #23=(CFSignal (CObList) "tetO" 21 0 1 6129 6147 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #24=(CFSignal (CObList) "tetO" 21 0 1 6213 6231 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #25=(CFSignal (CObList) "tetO" 21 0 1 6297 6315 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #26=(CFSignal (CObList) "tetO" 21 0 1 6339 6357 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "") 
COMMENT        #27=(CFSignal (CObList) "tetO" 21 0 1 6381 6399 0 (CStringList) 
COMMENT             (CStringList) 1 1 1 1 "")) (CObList) (CObList) (CObList) (CObList) 
COMMENT       (CObList 
COMMENT        #28=(CFSignal (CObList) "LMBP 10509" 2 0 0 0 0 0 (CStringList) 
COMMENT             (CStringList) 1 1 0 0 "") 
COMMENT        #29=(CFSignal (CObList) "Last modified: 14-JUL-2020" 2 0 0 0 0 0 
COMMENT             (CStringList) (CStringList) 1 1 0 0 "")) (CObList) 
COMMENT       (CTextView 0 
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COMMENT                     #33=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "DNA Plasmid Phage 'pNIM1A-tTA'" 1) 
COMMENT                     #34=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "LMBP 10509." 1) 
COMMENT                     #35=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Currently local object. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                          1) 
COMMENT                     #36=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Created: 01/19/18 09:44" 1) 
COMMENT                     #37=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Last Modified: 07/14/20 04:27" 1) 
COMMENT                     #38=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "length: 9273 bp" 1) 
COMMENT                     #39=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "storage type: Basic" 1) 
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COMMENT                          "form: Circular" 1))) "General Description") 
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COMMENT                          "Original Source Database: GenBank" 1) 
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COMMENT                          "Modification Date in the Original DB: 13-JUL-2020" 1) 
COMMENT                     #44=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "Associated Genes:  catTA;  CaSAT1;  GFP" 1))) 
COMMENT                   "Standard Fields") 
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COMMENT                          "Ghent University" 1) 
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COMMENT                          "Technologiepark 927" 1) 
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COMMENT                          "B-9052 Gent-Zwijnaarde Belgium" 1) 
COMMENT                     #50=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "TEL: 0032-9-33-13-843  " 1) 
COMMENT                     #51=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                          "EMAIL: <a href=\"mailto:bccm.genecorner@UGent.be\">bccm.genecorner@UGent.be</a>" 
COMMENT                          1) 
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COMMENT                          "WWW: <a href=\"http://www.genecorner.ugent.be\">www.genecorner.ugent.be</a>" 
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COMMENT                                 "" 1))) "LMBP 10509") 
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COMMENT                                   #80=(CLinePar 
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COMMENT                                  "3' CaADH1 integration site"))) 
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COMMENT                                   "CaADH1 promoter") 
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COMMENT                           "Promoter Eukaryotic (3 total)") 
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COMMENT                                   "CaACT1 promoter" "@N" 1 6.81704 -13.6541 0 
COMMENT                                   -10 7.93268 1.12054 #351#) 
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FEATURES             Location/Qualifiers
     promoter        627..643
                     /vntifkey="30"
                     /label=T7\promoter
     promoter        complement(7087..7103)
                     /vntifkey="30"
                     /label=T3\promoter
     promoter        complement(7167..7250)
                     /vntifkey="30"
                     /label=lac\promoter
     rep_origin      complement(7342..7744)
                     /vntifkey="33"
                     /label=pMB1\ori
     CDS             complement(8285..9145)
                     /vntifkey="4"
                     /label=ampicillin\resistance
     rep_origin      complement(4..459)
                     /vntifkey="33"
                     /label=f1\ori
     promoter        668..1532
                     /regulatory_class="promoter"
                     /vntifkey="29"
                     /label=CaADH1\promoter
     CDS             1533..2516
                     /gene="catTA"
                     /codon_start=1
                     /transl_table=12
                     /product="tetracycline regulated transactivator"
                     /protein_id="ART66047.1"
                     /vntifkey="4"
                     /label=CatTA
     terminator      2540..2861
                     /regulatory_class="terminator"
                     /vntifkey="43"
                     /label=CaQCR7\bidirectional\terminator
     CDS             complement(2898..3460,4115..4124)
                     /gene="CaSAT1"
                     /codon_start=1
                     /transl_table=12
                     /product="nourseothricin resistance marker"
                     /protein_id="ART66048.1"
                     /vntifkey="4"
                     /label=nourseothricin\resistance
     promoter        complement(4125..4622)
                     /regulatory_class="promoter"
                     /vntifkey="29"
                     /label=CaACT1\promoter
     terminator      complement(4744..5011)
                     /regulatory_class="terminator"
                     /vntifkey="43"
                     /label=CaACT1\terminator
     CDS             complement(5018..5734)
                     /gene="GFP"
                     /codon_start=1
                     /transl_table=12
                     /product="green fluorescent protein"
                     /protein_id="ART66049.1"
                     /vntifkey="4"
                     /label=CaGFP
     promoter        complement(5745..6399)
                     /regulatory_class="promoter"
                     /vntifkey="29"
                     /label=CaOPS4T\promoter
     misc_feature    6414..7060
                     /vntifkey="21"
                     /label=3'\CaADH1\integration\site
     misc_feature    complement(6255..6273)
                     /vntifkey="21"
                     /label=tetO
     misc_feature    complement(6171..6189)
                     /vntifkey="21"
                     /label=tetO
     misc_feature    complement(6129..6147)
                     /vntifkey="21"
                     /label=tetO
     misc_feature    complement(6213..6231)
                     /vntifkey="21"
                     /label=tetO
     misc_feature    complement(6297..6315)
                     /vntifkey="21"
                     /label=tetO
     misc_feature    complement(6339..6357)
                     /vntifkey="21"
                     /label=tetO
     misc_feature    complement(6381..6399)
                     /vntifkey="21"
                     /label=tetO
BASE COUNT     2827 a      1871 c      1884 g      2691 t 
ORIGIN
        1 ctgaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct 
       61 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg 
      121 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact 
      181 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac 
      241 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga 
      301 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga 
      361 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca 
      421 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca ttcaggctgc 
      481 gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag 
      541 ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt 
      601 gtaaaacgac ggccagtgag cgcgcgtaat acgactcact atagggcgaa ttggagctcc 
      661 accgcggttg agatggagcc gtcaaatatc caaaacactc ttctttcaac taatttgccg 
      721 taaactatct ccatcgtacg attaagataa aaaaaaacaa ttatttctta caatatttga 
      781 tagagaccca atgcaaagcc agcgagaaag aggagtattg gcattgttgg gtagtagatg 
      841 aatgagtaat cttctagcca aaacaaaata aaccacaaga acagtaggca aaactgagta 
      901 cttgatagcg cttgtagtct ttgtatttaa aaaaacctaa ttgtccaaaa attgaaaatc 
      961 atgagttgca aatttgttga aatcaccagg taaatagatc atttagctat atcgccttat 
     1021 cattcttcgt acaagaatta tacacaaata ccaccaccac accacccaga aattttacac 
     1081 gtgttttcta aattattact ttccgtggcc aatcaaattg aaatttttct tgttcgaaac 
     1141 ggcagtgcct caaacattat agtagcaagt attgcctaaa gagtttccgt ctcgtgcccc 
     1201 ccccctatcc ccttttgtaa tttgccaaaa tagctcaaaa aggataatcc cgcacttttt 
     1261 ttttccgagt tctcgtcatg caagcaagct tattcagaat tttcagaggt gcgagtctca 
     1321 ccactaccac tgcagctgca tcttttacag caacagcaac agcaggtgcc accacggcaa 
     1381 agacattgtc tggatccact gtgctccgaa aactgtataa aaggacctat tcatccctgg 
     1441 tcttatcttc tccagaatta tttttttttc atcagtttaa caacaacaaa cgttattgtc 
     1501 atacaacaac aacaacaaat acaaaaacaa ttatgtctag attagataaa agtaaagtga 
     1561 ttaacagcgc attagagttg cttaatgagg tcggaatcga aggtttaaca acccgtaaac 
     1621 tcgcccagaa gctaggtgta gagcagccta cattgtattg gcatgtaaaa aataagcggg 
     1681 ctttgctcga cgccttagca attgagatgt tagataggca ccatactcac ttttgccctt 
     1741 tagaagggga aagctggcaa gattttttac gtaataacgc taaaagtttt agatgtgctt 
     1801 tactaagtca tcgcgatgga gcaaaagtac atttaggtac acggcctaca gaaaaacagt 
     1861 atgaaactct cgaaaatcaa ttagcctttt tatgccaaca aggtttttca ctagagaatg 
     1921 cattatatgc actcagcgca gtggggcatt ttactttagg ttgcgtattg gaagatcaag 
     1981 agcatcaagt cgctaaagaa gaaagggaaa cacctactac tgatagtatg ccgccattat 
     2041 tacgacaagc tatcgaatta tttgatcacc aaggtgcaga gccagccttc ttattcggcc 
     2101 ttgaattgat catatgcgga ttagaaaaac aacttaaatg tgaaagtggg tcctcgactt 
     2161 tgtttggtgg cgccaatttt aatcaaagtg ggaatattgc tgatagctca ttgtccttca 
     2221 ctttcactaa cagtagcaac ggtccgaacc tcataacaac tcaaacaaat tctcaagcgc 
     2281 tttcacaacc aattgcctcc tctaacgttc atgataactt catgaataat gaaatcacgg 
     2341 ctagtaaaat tgatgatggt aataattcaa aaccattgtc acctggttgg acggaccaaa 
     2401 ctgcgtataa cgcgtttgga atcactacag ggatgtttaa taccactaca atggatgatg 
     2461 tatataacta tctattcgat gatgaagata ccccaccaaa cccaaaaaaa gagtaaggat 
     2521 ctaaattctg gaaatctaag taagtaagag gaggaggagg aggaagaaga tgagaaggtc 
     2581 aaacattgaa ataggaaaca agaaagcagg agacaagttt aaagaatgaa taataaatat 
     2641 tcttatgaaa gttatattta tttttgttag ttttctttta aatatattcg taatgtcaat 
     2701 ataaaacaat ttacaaaaag agattgtcaa aaagaagaaa cacatattat aatctaagtc 
     2761 tattaaatat ctctataaaa aaataatgtt atacttgaac aaaagcacta ttctttttgt 
     2821 ttatctatat ctatatatct atctatcaaa tatctttgtt tttaatctga tcgactcatt 
     2881 cctaatttaa acatatgtta ggcgtcatcc tgtgctcccg agaaccagta ccagtacatc 
     2941 gctgtttcgt tcgagacttg aggtctagtt ttatacgtga agaggtcaat gccgccgaga 
     3001 gtaaagccac attttgcgta caaattgcag gcaggtacat tgttcgtttg tgtctctaat 
     3061 cgtatgccaa ggagctgtct gcttagtgcc cactttttcg caaattcgat gagactgtgc 
     3121 gcgactcctt tgcctcggtg cgtgtgcgac acaacaatgt gttcgataga ggctagatcg 
     3181 ttccatgttg agttgagttc aatcttcccg acaagctctt ggtcgatgaa tgcgccatag 
     3241 caagcagagt cttcatcaga gtcatcatcc gagatgtaat ccttccggta ggggctcaca 
     3301 cttctggtag atagttcaaa gccttggtcg gataggtgca catcgaacac ttcacgaaca 
     3361 atgaaatggt tctcagcatc caatgtttcc gccacctgct cagggatcac cgaaattttc 
     3421 atatgagaac cgttatcgat aactaaagca gcaacttctt ctataaaaat gggttagtat 
     3481 gacagtcatt taaataagga atttttcagt tggcttggtt tcaattcaat gttcgttttt 
     3541 tttttttctt gctgtgtttg tgtttgtgtt gtttatagtt gtgtgcactg atcgtcgaaa 
     3601 aaaaaaattc atagtgagcc gggaaatctg tatagcccag ataacaacac aagtccaaac 
     3661 tagaaactcg tcaaacacca aaagcaatgt tgaatcaatt gccttgcaca agtacacgta 
     3721 ggaaaacata aaacattgca attttgaata ttgagccttt tgtcgtaaca ttgattgata 
     3781 ggattactca ccgaatggtt ttgaaaccac tgccgacaga tcaatcaatc aatcaaaaaa 
     3841 cgtgaacttt gaaaaagggg aagaacagat acattgaagt tagccatttc cactgatcgt 
     3901 cacaacatat ctgataaatt actttcaaaa ttataagctg atgtgtgtgt attattaatg 
     3961 tgacagtaac atcccaaacg agaaatatta tctcgacaac aaaaaagttt gatctgaatt 
     4021 gaaaatgaag ttttcccacc ctacccattt gtcatattga aaccaatcaa ctgattaatc 
     4081 aatcaattag aattgaagct aaactaaaac ataccaccgt ccattttgaa tgattatatt 
     4141 tttttaatat taatatcgag ataatgtttc taagaaagaa agaaaaccag gagtgaaaat 
     4201 tagaaaagga aaggaaagga aaaaaagaaa aatctgaaaa tatataaaaa aaaattgttt 
     4261 cgttggcaat aaatcttggt gagaacagcg accgaaagca aataagaaca aaatatgagt 
     4321 gtattacgtt gaacaactaa ttaacgtgtg tgtatggatc tttttttctt ttttctcttt 
     4381 aaccgactat aaacaacaaa catttttggg cagtgcacac actacttaat atacacagca 
     4441 taaattacac gattagaaac aaattagctt attaaaataa cctaatcaaa ccgaatattt 
     4501 tatggtatta tgagtaaact atataatata aatagcacac acccacaaca acaacaaagg 
     4561 aaaactaaaa ggttttttct ttttgaaaag atcgttttct ttattattct ctagttttga 
     4621 cgctcgacat tttatgatgg aatgaatggg atgaatcatc aaacaagaga aaatacccgt 
     4681 gacgaaaata ataaaataag ttcctctgat acagaagatg aaaacaacaa caacaagata 
     4741 tagaaatgcc ttgggtggct attttatagt cttaactttt taatgtatat ttgttttgtt 
     4801 tttttacata ataatacttt ataaaagcta agctaaattc aagtaaaatt tcaatctctc 
     4861 aaataaaaca tttttctctt tttcttaaat ttagttttat atatttataa aatatacaaa 
     4921 gattttttta aaaaagtaac aagttatata tgtaataaca aaaagaagaa taacaagaat 
     4981 acaaaaccag atttccagat ttccagaatt tagatcttta tttgtatagt tcatccatgc 
     5041 catgtgtaat cccagcagct gttacaaact caagaaggac catgtggtct ctcttttcgt 
     5101 tgggatcttt cgaaagggca gattgtgtgg acaagtaatg gttgtctggt aaaaggacag 
     5161 ggccatcgcc aattggagta ttttgttgat aatggtctgc tagttgaacg cttccatctt 
     5221 caatgttgtg tctaattttg aagttaactt tgattccatt cttttgtttg tctgccatga 
     5281 tgtatacatt gtgtgagtta tagttgtatt ccaatttgtg tccaagaatg tttccatctt 
     5341 ctttaaaatc aatacctttt aactcgattc tattaacaag ggtatcacct tcaaacttga 
     5401 cttcagcacg tgtcttgtag ttcccgtcat ctttgaaaaa tatagttctt tcctgtacat 
     5461 aaccttcggg catggcactc ttgaaaaagt catgccgttt catatgatct gggtatctcg 
     5521 caaagcattg aagaccatac gcgaaagtag tgacaagtgt tggccatgga acaggtagtt 
     5581 ttccagtagt gcaaataaat ttaagggtaa gttttccgta tgttgcatca ccttcaccct 
     5641 ctccactgac agaaaatttg tgcccattaa catcaccatc taattcaaca agaattggga 
     5701 caactccagt gaaaagttct tctcccttac tcatctttgt cgactattta tatttgtatg 
     5761 tgtgtaggag ttaacgtagt agtagacgta gttggtttta ttgtagttga tattgatttc 
     5821 tttctgagaa gttgaaagag tttgttgatt tgatttgata atgatataaa agaaataaaa 
     5881 aaaaatctaa aacttgaata caagagattt gaatctatat ttatatttca gaaaagccca 
     5941 tcagaaacga caaacatgga caacacttta attgaaaggg ctttattttt aagtgaagct 
     6001 ttttccttaa atcattgtta gtaattccca tagttattga atctattact caatcgagta 
     6061 gtttatattt aatttatcga caatattgcg aaaaataaaa aagaagtttg gagctcgact 
     6121 ttcacttttc tctatcactg atagggagtg gtaaactcga ctttcacttt tctctatcac 
     6181 tgatagggag tggtaaactc gactttcact tttctctatc actgataggg agtggtaaac 
     6241 tcgactttca cttttctcta tcactgatag ggagtggtaa actcgacttt cacttttctc 
     6301 tatcactgat agggagtggt aaactcgact ttcacttttc tctatcactg atagggagtg 
     6361 gtaaactcga ctttcacttt tctctatcac tgatagggag tggtaaactc gacaaggtgc 
     6421 tgaaccaaac tgtggtgaag ctgacttgtc tggttacact cacgatggtt cattcgaaca 
     6481 atacgctact gctgatgctg tccaagccgc taaaattcca gctggtactg atttagccaa 
     6541 tgtcgcacca atcttatgtg ctggtgttac tgtttacaaa gccttaaaga ctgctgactt 
     6601 agcagctggc caatgggttg ctatctccgg tgctggtggt ggtttaggtt ctttggccgt 
     6661 tcaatacgcc agagccatgg gtttgagagt tgtcgctatt gacggtggtg acgaaaaagg 
     6721 tgaatttgtt aaatcattgg gtgctgaagc ttacgttgat ttcaccaaag ataaagatat 
     6781 tgttgaagct gtcaagaaag ctactgatgg tggtccacac ggtgctatca atgtctctgt 
     6841 ctcagaaaga gccattggtc aatccactga atatgtcaga actttaggta aagttgtttt 
     6901 ggttggtttg ccagctggta ctaaaattag taccccagtc tttgatgctg ttatcaagac 
     6961 cattcaaatt aaaggttctt atgtcggtaa cagaaaagat actgctgaag ccgttgattt 
     7021 cttcacaaga ggtttgatca aatgcccaat caagattgtc gggcccggta cccagctttt 
     7081 gttcccttta gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg 
     7141 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta 
     7201 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg 
     7261 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga 
     7321 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg 
     7381 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag 
     7441 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc 
     7501 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca 
     7561 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt 
     7621 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc 
     7681 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc 
     7741 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc 
     7801 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact 
     7861 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg 
     7921 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta 
     7981 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca 
     8041 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa 
     8101 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg 
     8161 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc 
     8221 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg 
     8281 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat 
     8341 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg 
     8401 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa 
     8461 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca 
     8521 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc 
     8581 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt 
     8641 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa 
     8701 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat 
     8761 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct 
     8821 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga 
     8881 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag 
     8941 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga 
     9001 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca 
     9061 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg 
     9121 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc 
     9181 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag 
     9241 gggttccgcg cacatttccc cgaaaagtgc cac 
//
