LOCUS       pC2HB-Cdc3         10613 bp ds-DNA     circular SYN 15-APR-2019
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct

COMMENT     VNTDATE|874972800|
COMMENT     VNTDBDATE|874972800|
COMMENT     VNTNAME|pC2HB-Cdc3|
FEATURES             Location/Qualifiers
     misc_feature    321..2503
                     /label=CmLEU2
     misc_feature    2741..3854
                     /label=IntegSeq
     misc_feature    4499..5402
                     /label=KanR
     misc_feature    4528..4530
                     /label=KanR start codon
     promoter        5563..7002
                     /label=MET3p
     primer          6932..6950
                     /label=6372
                     /note="sequence: cttcaacttccaagtgttg"
     misc_feature    7003..7008
                     /label=BspEI
     misc_feature    7009..7011
                     /label=Start codon
     misc_feature    7021..7041
                     /label=SV40
     misc_feature    7045..7662
                     /label=LexA
     misc_feature    7687..7713
                     /label=HA tag
     misc_feature    7714..7731
                     /label=Glycine linker
     misc_feature    7744..9140
                     /label=Cdc3
     terminator      9169..9682
                     /label=CaACT1term
     misc_feature    9812..10485
                     /label=pUCori
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
      301 ctagtaacgg ccgccagtgt gctggaattc gcccttggat ccaatcatca ctggtggggg
      361 tcaagaaaga ggtttgagat caggtacttt ggcaccacca ttagtagctg ggtttggtga
      421 agctgcaaga ttaatgaaac aagaatctgc atttgacaag aaacatattg agaggttatc
      481 tactaaattg aagaacggtt tgttatccat tccatctact cagtttaacg gttgtaataa
      541 cccaacatat caatacccag gttgtgtgaa tgtttccttt gcatacattg aaggggaatc
      601 tttgttaatg gcattgaaag atattgcatt gagttctggt tctgcatgta cctctgcatc
      661 tttggaacca tcgtatgttt tacatgcttt gggtgccgat gatgccttgg ctcattcttc
      721 cattagattc ggtattggta gattcaccac tgaagcagaa gttgactatg ttattcaagc
      781 aattaatgaa agagttgatt tcttgagaaa gatgtctcca ttatgggaaa tggttcaaga
      841 aggtattgac ttgaactcta tcgaatggag tggacattaa ggttttttgt ttttttgtaa
      901 tttgtgttgt tatgtgtaat tatcgtgtat agagtaattt cggaaattgt actttcgttc
      961 atgtatctga accagatgaa taatacgttc gtattgttga agaatgacta actcctgtaa
     1021 cggcgtgcgt gggttccgca gccgggttca attttttttt tcttgttcac tataaaaata
     1081 tataataaac gaaaaaaatc cgagggactc aggctatgaa atttttcact caagaatttt
     1141 ttttccttca tccttaattc ctttattcac tacccaaacc cacaatgtca gttaaaacta
     1201 aaactattac aattttacca ggtgatcacg tcggtaccga aattgtcaat gaagctatca
     1261 aagttttaga agcaattgaa gctgccactc cttaccaaaa aatccatttc gatttcaaac
     1321 atcatttaat cggtggtgct gccattgatg ccactggtgt cccattgcca gatgacgcac
     1381 ttgaaagtgc caaaaaatct gatgccgtct tattgggtgc tgttggtgga ccaaaatggg
     1441 ggactggcgc cgttcgtcca gaacaaggtt tattaaagat tcgtaaagaa ttgaaccttt
     1501 atgctaacat cagaccatgt aactttgcca gtgactcctt attggaatta tccccattga
     1561 gaccagaagt cgttaaaggt acaaacttga tcattgttcg tgaattagtt ggtggtattt
     1621 atttcggtga tcgtgaagaa caagaagaaa gtgaagataa acaaaccgca tgggatactg
     1681 aaaaatacac cgtcgatgaa gtcaccagaa tcacccgtat ggctgccttc atggctttac
     1741 aacataaccc accattacca atctggtcat tggataaggc taatgtttta gcttcttcta
     1801 gattatggag aagaactgtc gataaagtga tttctgaaga gttcccaact ttgtctgttc
     1861 aacatcaatt gattgactcc gccgccatga ttttaattca aaacccaacc aaattgaatg
     1921 gtataatcat cacttctaac atgtttggtg atatcatttc cgatgaagct tcagttattc
     1981 caggttcctt gggtttatta ccatctgcat ctttggcttc attgccagat accaacaccg
     2041 cttttggtct ttatgaacca tgtcacggtt ctgctcctga tttaccagcc aataaagtta
     2101 acccaattgc tactatttta tctgctgctt ctatgttgag attatctttg gattgtgtga
     2161 aagaagctga agctttagaa gaagccgtga aacaagtctt ggataaaggt atcagaactg
     2221 cagatttaag aggtaccagt tcagctactg aagttggtga cgcgattgtc gaagctgtta
     2281 ctaaaatctt aaaagaaaaa gcttaagtaa agtatacaca aaatttaacg aatattatta
     2341 atacaaaatg aacaccacct aaatactttt ttttaaaaat gtaccgactc ttctaaaaat
     2401 tgcgataatt caatatcgga aatttctctc accggtttac ttggatcttc ggaaataaat
     2461 ctatttgaag taatggtagc ctcacttgat tctgattggc tctttctaga catgggtagg
     2521 taagggcgaa ttctgcagat atccatcaca ctggcggccg gccgctcgag catgcatcta
     2581 gagggcccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
     2641 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     2701 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg accagatcaa aatgctagaa
     2761 caaattacgt ctgcatcaac agtcatcttc cttatttgca taaatgctaa aatggcaaac
     2821 ctgtaaaccg tttgaaaagc aaattgagat agttagctcg gttttttttt cacgtggcca
     2881 taatccaaca aaaaaaagga atttaagggg ggagagaagg aggcttgaat ttgtaccaca
     2941 gagcgagcaa gaaattaata atatacttgg cttagctgaa atgagaatga aaaagcaaat
     3001 ttcaagtgaa acaccactac tagccaagta aattggccaa tttgctctcg tgctttaatt
     3061 tcctaacaag cttttgagac cgaaaaaaat caaccaatga tgatggaaaa atacttccgg
     3121 gagtcaattt atccataatt tcacgtcgtt ttttttttgc acatggcaaa tatattggaa
     3181 tcctgagatt ttcttggagt cgtgtacaat aacaccaggc gggaacatgc cgttatgatc
     3241 cagcatagcg gccgctatgt gttcaaatat tgaaaccgtg aaaccagttt gatacagctg
     3301 atattgtgtg ctaaaaaaag atgagcaccg aaagagtctt tagtttattg ccaagtaaag
     3361 ggtcaaagaa tacctattct ttatgaacac agacttgctc taatggtgaa ttagtgtgga
     3421 gtaatagaga aggaggcgaa acaaaaataa ttggttaaac actcgaatgt cacacaatcc
     3481 taaataggtt aaattcagtt accgccgtga tgcgggaaca aagccgagcg gtaatatttt
     3541 tggattctat gtttttttgg tgtatattgc acgacttggt gccaacaact ggtgttccaa
     3601 cgcacaaaac taggttcaac tggatactgt tttctagaaa ttatttgcgg caattcatga
     3661 ttacggtcct aaatttgaaa taaaagtttc caattatgtg tcttgacaat ttccattggg
     3721 ttcaaaaaga tctagaatgt atgtaattac gcaacaattt gcatgcaaag aaataaaaac
     3781 aaacctaaaa tccaactgct gttgttctgg tgatgatgta gtaaaataag agcacggcca
     3841 agaacaaggt gatttacgta cggcagttta aggtttacac ctataaaaga gagagccgtt
     3901 atcgtctgtt tgtggatgta cagagtgata ttattgacac gccggggcga cggatggtga
     3961 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg
     4021 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct
     4081 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
     4141 ttaacctgat gttctgggga atataaatgt caggcatgag attatcaaaa aggatcttca
     4201 cctagatcct tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg
     4261 tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt
     4321 gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg
     4381 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg
     4441 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag ctctgatcaa gagacaggat
     4501 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     4561 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     4621 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     4681 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     4741 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     4801 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     4861 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     4921 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     4981 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     5041 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     5101 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     5161 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     5221 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     5281 tctatcgcct tcttgacgag ttcttctgaa ttattaacgc ttacaatttc ctgatgcggt
     5341 attttctcct tacgcatctg tgcggtattt cacaccgcat caggtggcac ttttcgggga
     5401 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5461 atgagacaat aaccctgata aatgcttcaa taatagcacg tgaggagggc caccatggcc
     5521 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcttccaatt agaggttgtg
     5581 gatgcaaaat tgaaaaaaaa atgactattg gtagcgtgtt ctatttttct tatgcgctga
     5641 aaaactacga acaattgtct attccaagcc tgtggctttt tttggtgatt cacaatacat
     5701 aaattggcaa atacctcttc tatagacttt aacctttgat gaaatggtat ggataaacca
     5761 cttttgtggt cagggtatat atatttttat tccaatctgg cctttataaa ttcaaatttc
     5821 caaggggact ctgctaaacc ttctgatata actataactc taataatggc tgtaaaaagt
     5881 gtgaaaaaaa gatgatattc acaaaaaaga taaaggggat ttcatattgg gtatctagat
     5941 ttaaacatcg tatttcaaca atgaaactag aatcaatacg aaatccaggg cacaactgtg
     6001 ttcattccct tgggaattat actgagtaat gggaagaatg tgcaaaaaat acccgtgtct
     6061 tatataattt ccatttgtgt agggcttaaa agtggacaac ttcaggttac gaaaccacga
     6121 aaatgtctaa acgtttgatc aaattcgtaa ctcatggaaa atcacagcaa caaattaagg
     6181 atggatatat ccggtactta caaataaaaa cactaaagaa gtataaattt tgaactgaaa
     6241 ttctatagaa aatctttttt tttgggtaaa attatgcgat tgtggctcat agtaacgtaa
     6301 tcatacagtt caacaaacat cattttgtca aaggatttct aatcctattc tttttctact
     6361 tggcttttga gtatatatat atatatatat acatcctttg ttcatcatca tgttcgattg
     6421 aatttttttc ttggttgtta ttagattgtc tacttgagtt tttcttggtg caaattgtgg
     6481 tgtttttcta acaaattttt caacatttaa agtatttctg gaacttttct acaaggaacg
     6541 acatagatcc attaatgcgc ccctctaaaa caatacccat aatgtcttct taagtatgca
     6601 tctgcttata aatgtttctg gatttacttt agttagaatg tcgacacgac aatgaactat
     6661 tgctgtagat cacgtgcgat cactgaatta tgagtttctt tttatttttc tttttgggct
     6721 tttttttttg caattttttt tttccacctt tgccaattgc gtagtcacat ataatgtata
     6781 caatttttgc aactctgaca ctataattga tccaataata aagtatataa tagatcatca
     6841 agtatacgta atctccccaa aaaaaaaatt gcataaaata aaatctataa acctttttta
     6901 gatttttagt tgttcctttt ttctttctat tcttcaactt ccaagtgttg tcactttctt
     6961 cctgttaact atatttaaaa gtaaataccc tccccagaaa actccggaat ggctgctcct
     7021 ccaaaaaaga agagaaaggt agctagagaa ttaacaaaac gacaaagcga aatatataac
     7081 tatattaaac aagttgttca aatgaaaggt tatccgccta gtgttcgcga aattggtgag
     7141 gcagttggct tagcatccag ttcaactgtt catggtcacc tttcacgtct tgaagaaaaa
     7201 ggctatataa gaagagatcc aacgaaacca cgtgctatag aaattgtaag tgatcaaaca
     7261 aatgataata ttaatatgga agaaacgatt catgtgccag ttattggtaa agtcacagca
     7321 ggtgttccta ttaccgcagt agaaaatatt gaagaatatt ttccattacc tgaacactta
     7381 acatcgacac acaatagcga catattcata ttaaacgtcg taggcgacag tatgattgag
     7441 gctggtatat tagacggaga caaagtaatt gttcgcagtc aaaccatagc agaaaatgga
     7501 gacattattg ttgctatgac tgaggaagat gaagcaactg tcaaacgctt ctataaagaa
     7561 aaaaatcgtt atcgattaca acctgaaaat agtacaatgg agccaattta cctcgacaat
     7621 gttgctgtaa tcgggaaagt aattggtttg taccgcgaaa tgagatctgg tgctggtggt
     7681 gttggttatc cgtatgatgt gcctgactac gcaggtgctg gtggtgttgg tgctagcagg
     7741 cctatggctg caggtatgta tattactact actactaata atggtattga ttgatcggta
     7801 atggtagatc aatcatctat aatgacaaga aatcttacta acaaattgtt atttctaata
     7861 tagttgatca ttctaccaca ggagaaattg ttccacaaaa tataaaaatc attaaaaaag
     7921 tgttaaatgg ttatgttggg tttgccaatt taccaaaaca atggcataga aaatctatta
     7981 gaagaggatt cagtttgaac attatggcca ttggtgaaag tggattgggt aaagccacat
     8041 tgattaatac tttgtttaac cgtgatatta taaccctgca acacgattcc gatgaatttg
     8101 acgaaggtga agaagaagat gtttctgtca agatcaaatc aactcaagca gaaattgaag
     8161 aagatggtgt caagttgaaa gtaagtgtta ttactgctcc tggatttggt gaatcaatca
     8221 ataatgtcga ggcttggaaa ccaattgttg atgaaatcaa tagtagattt gattcttatt
     8281 tagaagcaga atcaagaatt aacagaactg ctgttgttga caatagagtt cacgcttttc
     8341 tttatttcat tgaaccaact ggtcattcat tgagagcttt agatattgca ttgatgaagc
     8401 aagttcatga aaaagtcaat ttgattccag ttattgctaa atctgatact ttgactgatg
     8461 aagaaatttt agagttcaaa cacaggatat tagctgatat tagtcatcaa ggaatcaaaa
     8521 tttttaaacc aactgatttt gaatatgatg aagaagaatc cgctaacact agactgatca
     8581 tagatagttt cccatttgcc gttgttggtt caactaatga agttcaaact cctgatggtc
     8641 gtttagttag aggtagaaaa tatccatggg gggttattga agttgataat gaaaaccaca
     8701 atgactttgt taaattacgc caattgttgg ttagaaactt tttagaggaa ttaaaagaac
     8761 atactgccaa tgttctttat gaaaattatc gaactgaaaa attgaagaga atgggtattg
     8821 aacaagataa tactgttttc agagaatttg atcctgctgc taaacaagaa gaagaaagag
     8881 ctttacatga agctaaatta gccaagatgg aagcagaaat gaaactggtt ttccaacaaa
     8941 aagtgagtga aaaggaaaag aaattacaaa gatctgaagc tgatttattt gccagacata
     9001 aagaaatgaa agataaattg actaaacaaa tcaaattatt agaagagaaa aaggctcaat
     9061 tagaaaaaca aaaattatta ccacaagacc caccagcaca accagctcca caaaagagtc
     9121 gtaaaggatt tttacgttag acgcgtagta cttaaccgcg ccgtcgacga gtgaaattct
     9181 ggaaatctgg aaatctggtt ttgtattctt gttattcttc tttttgttat tacatatata
     9241 acttgttact tttttaaaaa aatctttgtt tattttataa atatataaaa ctaaatttaa
     9301 gaaaaagaga aaaatgtttt atttgagaga ttgaaatttt acttgaattt agcttagctt
     9361 ttataaagta ttattatgta aaaaaacaaa acaaatatac attaaaaagt taagactata
     9421 aaatagccac ccaaggcatt tctatatctt gttgttgttg ttttcatctt ctgtatcaga
     9481 ggaacttatt ttattatttt cgtcacgggt attttctctt gtttgatgat tcatcccatt
     9541 cattccatca taaaattcta gaaggaccac ctttgattgt aaatagtaat aattaccacc
     9601 cttatttaat tatttattta acttatttat ttatttatta tacatatata caaatctaat
     9661 aaagtgaaaa tctccccctt cacacgtggc cgaggagcag gactgacacg tgctaaaact
     9721 tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat
     9781 cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc
     9841 ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct
     9901 accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg
     9961 cttcagcaga gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca
    10021 cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc
    10081 tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga
    10141 taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac
    10201 gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga
    10261 agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag
    10321 ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg
    10381 acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag
    10441 caacgcggcc tttttacggt tcctgggctt ttgctggcct tttgctcaca tgttctttcc
    10501 tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc
    10561 tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aag
//
