LOCUS       pAT153cI857             4129 bp    DNA     circular     19-MAR-1980
DEFINITION  LMBP 1065.
SOURCE      
  ORGANISM  
COMMENT     VNTNAME|pAT153cI857|
COMMENT     VNTOAUTHORNAME|BCCM/GeneCorner Plasmid Collection|
COMMENT     VNTOAUTHORTEL|0032-9-33-13-843|
COMMENT     VNTOAUTHOREML|bccm.genecorner@UGent.be|
COMMENT     VNTOAUTHORWWW|www.genecorner.ugent.be|
COMMENT     VNTOAUTHORAD1|Ghent University|
COMMENT     VNTOAUTHORAD2|Technologiepark 927|
COMMENT     VNTOAUTHORAD3|B-9052 Gent-Zwijnaarde Belgium|

COMMENT     Vector_NTI_Display_Data_(Do_Not_Edit!)
COMMENT     (SXF 
COMMENT      (CGexDoc "pAT153cI857" 0 4129 
COMMENT       (CDBMol 0 0 1 1 1 0 0 1 0 "" "" 0 0 0 0 (CObList) (CObList) (CObList) 
COMMENT        (CObList) -1 "") 
COMMENT       (CDocSetData 1 1 0 0 1 0 " Anneleen" 1 1 1 1 0 0 1 1 0 1 10 6 4000 50 0 1 
COMMENT        0 (CHomObj 0 0 0 3 85) (CWordArray 0 4 15 20 21 22 29 30 33 43 62 32 59) 
COMMENT        (CWordArray) (CStringList) (CStringList "atg" "gtg") 
COMMENT        (CStringList "taa" "tga" "tag") 
COMMENT        (CObList 
COMMENT         #0=(COligo "CaURA3" 
COMMENT             "ATGACAGTCAACACTAAGACCTATAGTGAGAGAGCAGAAACTCATGCCTCACCAGTAGCACAGCGATTATTTCGATTAATGGAACTGAAGAAAACCAATTTATGTGCATCAATTGACGTTGATACCACTAAGGAGTTCCTCGAGTTAATTGATAAATTAGGTCCTTATGTATGCTTAATCAAGACTCATATTGATATAATCAATGATTTTTCCTATGAATCCACTATTGAACCATTATTAGAACTTTCACGTAAACATCAATTTATGATTTTTGAAGATAGAAAATTTGCTGATATTGGTAATACCGTAAAGAAACAATATATTGGTGGAGTTTATAAAATTAGTAGTTGGGCAGATATTACCAATGCTCATGGTGTCACTGGGAATGGAGTGGTTGAAGGATTAAAACAGGGAGCTAAAGAAACCACCACCAACCAAGAGCCAAGAGGGTTATTGATGTTAGCTGAATTATCATCAGTGGGATCATTAGCATATGGAGAATATTCTCAAAAAACTGTTGAAATTGCTAAATCCGATAAGGAATTTGTTATTGGATTTATTGCCCAACGTGATATGGGTGGCCAAGAAGAAGGATTTGATTGGCTTATTATGACACCTGGAGTTGGATTAGATGATAAAGGTGATGGATTAGGACAACAATATAGAACTGTTGATGAAGTTGTTAGCACTGGAACTGATATTATCATTGTTGGTAGAGGATTGTTTGGTAAAGGAAGAGATCCAGATATTGAAGGTAAAAGGTATAGAAATGCTGGTTGGAATGCTTATTTGAAAAAGACTGGCCAATTATAA" 
COMMENT             "Fragment of pGAL1PSiST-1; Length: 813" 0 (CStringList) 0) 
COMMENT         #1=(COligo "PpURA3" 
COMMENT             "ATGGCTCGCAGTTATGCCGAGAGAGCAAATACTCATCAATCACCTGTGGCACGACGACTGTTTGCGCTTATGGAACAGAAACAGAGTAACCTATGCGCATCAGTCGACGTGAGAACAACTAAAGAATTATTGGAGCTTCTAGATAAATTGGGCCCATTTATCTGTTTGGCCAAGACTCATATCGACATAATTGATGACTTCACGTATGATGGAACTATTCTGCCTTTATTGGAACTATCAAAGAAACACAAGTTTTTAATTTTTGAGGACAGAAAGTTTGCTGATATAGGCAACACTGTCAAGCATCAATATCAAGGAGGTGTCTACAAGATTGCACAATGGGCAGATATTACAAATGCTCATGGTGTCATTGGTAGTGGAATTGTAAAGGGTCTAAAGGAGGCAGCCACTGAGACAACAGATCAACCAAGGGGACTATTGATGTTGGCTGAACTGTCGTCAAAGGGATCAATTGCCCATGGTAAGTACACCGAAGAAACTGTAGAAATTGCAAAATCAGACAAGGAACTCGTCATTGGGTTTATTGCTCAAAATTCTATGGGAGGACAAGATGAAGGGTTCGATTGGATTATTATGACACCAGGTGTTGGTTTGGATGACACTGGTGATGCTCTAGGCCAACAATATCGAACAGTGAGTCAAGTATTTTCCACTGGCACTGACATCATAATCGTAGGTCGTGGTTTGTTTGGCAAGGGCAGAGATCCCTTAAAAGAAGGTGAACGGTATAGAAAAGCTGGGTGGGAAGCTTACCAAAATATTCTGAGGTAA" 
COMMENT             "Fragment of pGlycoSwitchM5-URA3; Length: 792" 0 (CStringList) 0) 
COMMENT         #2=(COligo "URA3" 
COMMENT             "ATGTCGAAAGCTACATATAAGGAACGTGCTGCTACTCATCCTAGTCCTGTTGCTGCCAAGCTATTTAATATCATGCACGAAAAGCAAACAAACTTGTGTGCTTCATTGGATGTTCGTACCACCAAGGAATTACTGGAGTTAGTTGAAGCATTAGGTCCCAAAATTTGTTTACTAAAAACACATGTGGATATCTTGACTGATTTTTCCATGGAGGGCACAGTTAAGCCGCTAAAGGCATTATCCGCCAAGTACAATTTTTTACTCTTCGAAGACAGAAAATTTGCTGACATTGGTAATACAGTCAAATTGCAGTACTCTGCGGGTGTATACAGAATAGCAGAATGGGCAGACATTACGAATGCACACGGTGTGGTGGGCCCAGGTATTGTTAGCGGTTTGAAGCAGGCGGCAGAAGAAGTAACAAAGGAACCTAGAGGCCTTTTGATGTTAGCAGAATTGTCATGCAAGGGCTCCCTATCTACTGGAGAATATACTAAGGGTACTGTTGACATTGCGAAGAGCGACAAAGATTTTGTTATCGGCTTTATTGCTCAAAGAGACATGGGTGGAAGAGATGAAGGTTACGATTGGTTGATTATGACACCCGGTGTGGGTTTAGATGACAAGGGAGACGCATTGGGTCAACAGTATAGAACCGTGGATGATGTGGTCTCTACAGGATCTGACATTATTATTGTTGGAAGAGGACTATTTGCAAAGGGAAGGGATGCTAAGGTAGAGGGTGAACGTTACAGAAAAGCAGGCTGGGAAGCATATTTGAGAAGATGCGGCCAGCAAAACTAA" 
COMMENT             "Fragment of pEMBLyex4; Length: 804" 0 (CStringList) 0)) 1 
COMMENT        "{(0,1),2}" 0 0 "" 0 4294967295 0 0 0 0 0 0 1 "MAIN" 0 0 30 0 
COMMENT        (CProteinMotifSearchObject 100 10 1 1 1 1 1 0 1 0 0 0 0 0)) 
COMMENT       (CMolPar 0 0 0 0 0 1 4129 0 0 0 0 0 0 0 0) (CStringList) (CStringList) 
COMMENT       (CObList) (COAPar 25 250 50 0 6 4 3 7) (COAPar 25 250 50 0 6 4 3 7) 
COMMENT       (COAPar 25 250 50 0 6 4 3 7) (CObList) 
COMMENT       (CObList 
COMMENT        #3=(CFSignal (CObList) "PRM" 30 0 0 35 90 0 (CStringList) (CStringList) 
COMMENT            1 1 1 1 "35..90") 
COMMENT        #4=(CFSignal (CObList) "PR" 30 0 1 9 75 0 (CStringList) (CStringList) 1 
COMMENT            1 1 1 "complement(9..75)") 
COMMENT        #5=(CFSignal (CObList) "ORI" 33 0 1 2120 2520 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(2120..2520)") 
COMMENT        #6=(CFSignal (CObList) "AMP" 4 0 1 3061 3921 0 (CStringList) 
COMMENT            (CStringList) 1 1 1 1 "complement(3061..3921)") 
COMMENT        #7=(CFSignal (CObList) "lambda cl857" 4 0 0 91 804 0 (CStringList) 
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COMMENT                         "DNA 'pAT153cI857'" 1) 
COMMENT                    #12=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Foreign object. Author: UNKNOWN. Original author: BCCM/GeneCorner Plasmid Collection" 
COMMENT                         1) 
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COMMENT                         "Creation Date: ? (no data)" 1) 
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COMMENT                         "Last Modification Date: ? (no data)" 1) 
COMMENT                    #15=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "length: 4129 bp" 1) 
COMMENT                    #16=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "storage type: Basic" 1) 
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COMMENT                         "form: Circular" 1))) "General Description") 
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COMMENT                         "Original Source Database: GenBank" 1) 
COMMENT                    #20=(CLinePar (CParagraph 0 (0 0) 1 2 1 0 180) 
COMMENT                         "Modification Date in the Original DB: 19-MAR-1980" 1))) 
COMMENT                  "Standard Fields") 
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COMMENT                  "Annotations") 
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COMMENT                   (CObjectList 
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COMMENT                                 (CObjectList 
COMMENT                                  #26=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "Start: 91    End: 804 " 1))) 
COMMENT                                "lambda cl857") 
COMMENT                           #27=(CFolderPar 
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COMMENT                                       "Start: 3061  End: 3921 (Complementary)" 
COMMENT                                       1) 
COMMENT                                  #29=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "antibiotic resistance gene; coding sequence" 
COMMENT                                       1) 
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COMMENT                                       "Original Location Description:" 1) 
COMMENT                                  #31=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "   complement(3061..3921)" 1))) "AMP"))) 
COMMENT                         "CDS (2 total)") 
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COMMENT                                       "Start: 9     End: 75   (Complementary)" 
COMMENT                                       1) 
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COMMENT                                       "phage \361 major rightward promoter" 1) 
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COMMENT                                       "Original Location Description:" 1) 
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COMMENT                                  #40=(CLinePar (CParagraph 0 (0 0) 1 2 3 0 180) 
COMMENT                                       "phage \361 promoter for repressor maintenance" 
COMMENT                                       1) 
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COMMENT                                       "Original Location Description:" 1) 
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COMMENT                         "Promoter Prokaryotic (2 total)") 
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COMMENT                                       "Original Location Description:" 1) 
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FEATURES             Location/Qualifiers
     promoter        35..90
                     /vntifkey="30"
                     /label=PRM
                     /note="phage ñ promoter for repressor maintenance"
     promoter        complement(9..75)
                     /vntifkey="30"
                     /label=PR
                     /note="phage ñ major rightward promoter"
     rep_origin      complement(2120..2520)
                     /vntifkey="33"
                     /label=ORI
                     /note="origin plasmid"
     CDS             complement(3061..3921)
                     /vntifkey="4"
                     /label=AMP
                     /note="antibiotic resistance gene; coding sequence"
     CDS             91..804
                     /vntifkey="4"
                     /label=lambda\cl857
BASE COUNT      979 a      1070 c      1086 g       994 t 
ORIGIN
        1 ttccacatgc aaccattatc accgccagag gtaaaatagt caacacgcac ggtgttagat 
       61 atttatccct tgcggtgata gatttaacgt atgagcacaa aaaagaaacc attaacacaa 
      121 gagcagcttg aggacgcacg tcgccttaaa gcaatttatg aaaaaaagaa aaatgaactt 
      181 ggcttatccc aggaatctgt cgcagacaag atggggatgg ggcagtcagg cgttggtgct 
      241 ttatttaatg gcatcaatgc attaaatgct tataacgccg cattgcttgc aaaaattctc 
      301 aaagttagcg ttgaagaatt tagcccttca atcgccagag aaatctacga gatgtatgaa 
      361 gcggttagta tgcagccgtc acttagaagt gagtatgagt accctgtttt ttctcatgtt 
      421 caggcaggga tgttctcacc tgagcttaga acctttacca aaggtgatgc ggagagatgg 
      481 gtaagcacaa ccaaaaaagc cagtgattct gcattctggc ttgaggttga aggtaattcc 
      541 atgaccgcac caacaggctc caagccaagc tttcctgacg gaatgttaat tctcgttgac 
      601 cctgagcagg ctgttgagcc aggtgatttc tgcatagcca gacttggggg tgatgagttt 
      661 accttcaaga aactgatcag ggatagcggt caggtgtttt tacaaccact aaacccacag 
      721 tacccaatga tcccatgcaa tgagagttgt tccgttgtgg ggaaagttat cgctagtcag 
      781 tggcctgaag agacgtttgg ctgatcggca aggtgttctg gtcggcgggg gggggggggg 
      841 gggggggatc ctctacgccg gacgcatcgt ggccggcatc accggcgcca caggtgcggt 
      901 tgctggcgcc tatatcgccg acatcaccga tggggaagat cgggctcgcc acttcgggct 
      961 catgagcgct tgtttcggcg tgggtatggt ggcaggcccc gtggccgggg gactgttggg 
     1021 cgccatctcc ttgcatgcac cattccttgc ggcggcggtg ctcaacggcc tcaacctact 
     1081 actgggctgc ttcctaatgc aggagtcgca taagggagag cgtcgaccga tgcccttgag 
     1141 agccttcaac ccagtcagct ccttccggtg ggcgcggggc atgactatcg tcgccgcact 
     1201 tatgactgtc ttctttatca tgcaactcgt aggacaggtg ccggcagcgc tctgggtcat 
     1261 tttcggcgag gaccgctttc gctggagcgc gacgatgatc ggcctgtcgc ttgcggtatt 
     1321 cggaatcttg cacgccctcg ctcaagcctt cgtcactggt cccgccacca aacgtttcgg 
     1381 cgagaagcag gccattatcg ccggcatggc ggccgacgcg ctgggctacg tcttgctggc 
     1441 gttcgcgacg cgaggctgga tggccttccc cattatgatt cttctcgctt ccggcggcat 
     1501 cgggatgccc gcgttgcagg ccatgctgtc caggcaggta gatgacgacc atcagggaca 
     1561 gcttcaagga tcgctcgcgg ctcttaccag cctaacttcg atcactggac cgctgatcgt 
     1621 cacggcgatt tatgccgcct cggcgagcac atggaacggg ttggcatgga ttgtaggcgc 
     1681 cgccctatac cttgtctgcc tccccgcgtt gcgtcgcggt gcatggagcc gggccacctc 
     1741 gacctgaatg gaagccggcg gcacctcgct aacggattca ccactccaag aattggagcc 
     1801 aatcaattct tgcggagaac tgtgaatgcg caaaccaacc cttggcagaa catatccatc 
     1861 gcgtccgcca tctccagcag ccgcacgcgg cgcatctcgg gcagcgttgg gtcctggcca 
     1921 cgggtgcgca tgatcgtgct cctgtcgttg aggacccggc taggctggcg gggttgcctt 
     1981 actggttagc agaatgaatc accgatacgc gagcgaacgt gaagcgactg ctgctgcaaa 
     2041 acgtctgcga cctgagcaac aacatgaatg gtcttcggtt tccgtgtttc gtaaagtctg 
     2101 gaaacgcgga agtcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt 
     2161 tcggctgcgg cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc 
     2221 aggggataac gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa 
     2281 aaaggccgcg ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa 
     2341 tcgacgctca agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc 
     2401 ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc 
     2461 cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag 
     2521 ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga 
     2581 ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc 
     2641 gccactggca gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac 
     2701 agagttcttg aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg 
     2761 cgctctgctg aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca 
     2821 aaccaccgct ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa 
     2881 aggatctcaa gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa 
     2941 ctcacgttaa gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt 
     3001 aaattaaaaa tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag 
     3061 ttaccaatgc ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat 
     3121 agttgcctga ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc 
     3181 cagtgctgca atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa 
     3241 ccagccagcc ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca 
     3301 gtctattaat tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa 
     3361 cgttgttgcc attgctgcag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt 
     3421 cagctccggt tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc 
     3481 ggttagctcc ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact 
     3541 catggttatg gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc 
     3601 tgtgactggt gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg 
     3661 ctcttgcccg gcgtcaacac gggataatac cgcgccacat agcagaactt taaaagtgct 
     3721 catcattgga aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc 
     3781 cagttcgatg taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag 
     3841 cgtttctggg tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac 
     3901 acggaaatgt tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg 
     3961 ttattgtctc atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt 
     4021 tccgcgcaca tttccccgaa aagtgccacc tgacgtctaa gaaaccatta ttatcatgac 
     4081 attaacctat aaaaataggc gtatcacgag gccctttcgt cttcaagaa 
//
