LOCUS p416-HAH2-Ptet 4481 bp DNA linear SYN 30-MAY-2017 DEFINITION LMBP 11189. ACCESSION MF048861 VERSION MF048861.1 KEYWORDS . SOURCE synthetic construct (code 12) ORGANISM synthetic construct (code 12) other sequences; artificial sequences. REFERENCE 1 (bases 1 to 4481) AUTHORS Bijlani,S. and Ganesan,K. TITLE Single-Transformation Gene Function Test by Conditional Expression of Both Alleles of Candida albicans Genes JOURNAL Unpublished REFERENCE 2 (bases 1 to 4481) AUTHORS Bijlani,S. and Ganesan,K. TITLE Direct Submission JOURNAL Submitted (04-MAY-2017) Yeast Molecular Biology, CSIR- Institute of Microbial Technology, Sector 39-A, Chandigarh, Chandigarh 160036, India COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..4481 /organism="synthetic construct (code 12)" /mol_type="other DNA" /db_xref="taxon:2005482" /clone="p416-HAH2-Ptet" misc_feature 22..55 /note="LoxLE" gene 60..928 /gene="HIS1" /note="5'region; derived from Candida albicans HIS1" repeat_region 575..928 /gene="HIS1" /rpt_type=direct gene 934..2884 /gene="ARG4" /note="derived from Candida albicans ARG4" CDS 1391..2797 /gene="ARG4" /codon_start=1 /transl_table=12 /product="argininosuccinate lyase" /protein_id="ART66073.1" /translation="MSQQQDKQPSENKLWGGRFTGATDPLMDLYNASLPYDKVMYDAD LTGTKVYTQGLNKLGLITTEELNLIHQGLEQIRQEWHDNKFIIKAGDEDIHTANERRL GEIIGKNISGKVHTGRSRNDQVATDMRIFVRESLLNLSKILHQFITAILERAHKEIDV LMPGYTHLQKAQPIRWAHWLSSYATYFTEDYKRLQEIITRVNQSPLGSGALAGHPYGI DREFLAKGLGFDGVIGNSLTAVSDRDFVVESLFWSTLFMNHISRFSEDLIIYSSGEFG FIKLADAYSTGSSLMPQKKNPDSLELLRGKSGRVFGQLSGFLMSIKSIPSTYNKDMQE DKEPLFDALTTVEHSILIATGVISTLSIDKQNMEKALTMDMLATDLADYLVRKGVPFR ETHHISGECVRKAEEEKLSGIDQLSFEQFQQIDSRFEKDVMETFDFEASVERRDALGG TAKSAVLKQLENLKSILS" gene 2889..3738 /gene="HIS1" /note="3'region; derived from Candida albicans HIS1" repeat_region 2889..3242 /gene="HIS1" /rpt_type=direct misc_feature 3739..3772 /note="LoxRE" regulatory 3800..4093 /regulatory_class="promoter" /note="seven copies of tet operator sequences" regulatory 4103..4468 /regulatory_class="promoter" /note="CaOP4 minimal promoter" misc_feature 4479..4481 /note="Start codon" ORIGIN 1 cttcgtacgc tgcaggtcga ctaccgttcg tataatgtat gctatacgaa gttatggatc 61 ctggaggatg aggagacaga agttagtagt aacaatttgg ggacagaaga ggatacagtg 121 acatcagagg aagaagacag cgaaggtata gtcaaaaagc aataggacag caagacaatt 181 tatttattta atagtttttt ataaatgtaa aatctatcac aacatgtgta cttaattttc 241 tgtggtaggg gaggccattt cgttgaaacg tgtcgcgagc ggttgtgaga attgactcat 301 tttgattatt acgcaaccat tgaaattttt tatttttttt tggtgaagat ttttcccaca 361 caacttcttc ttttacttaa ccaccaacta ccgataatgg atttagtcaa tcatttacca 421 gaccgtttgt tatttgctgt tcctaaaaag ggcagattat acgaaaaatg ctgtaactta 481 ttgagtggtg ccgatataca gtttagaaga tctaatagat tagatatagc actttctaca 541 aacttgccaa ttgcattaat cttcttgcct gcagctgata tcccagtttt cgttggagaa 601 ggtaattgtg acttgggtat aactgggtta gaccaaatca aagaagctga acaattcgac 661 aacatcgagg acttgttgga tttgaaattt ggttcatgca aattgcagat ccaagttcca 721 gcagatggcg agtacgaaaa gccagaacag cttgttggaa agaaaattgt gtcttcattt 781 acaaaattga gtaccgacta tttcaaacaa ttgtcagaca aacctactaa tatcagatat 841 gtcggtggtt ccgttgaggc ttcttgtgcc ttgggtgttg ctgatgctat tgtcgatttg 901 gttgaaagtg gtgaaactat gaaagcagga tccccccttt agtaagattt ttcaagagta 961 gtctcaaata aacagaatga aggattgaag gttttattca ataaataatt ggacagaaag 1021 ttattgcaca gttttaacaa caacaatttc aaaatttcaa tttatgacat attgaccgac 1081 ataattcagt catttctaat attcaaataa caagacacct tgtataaatg aatccacaag 1141 cgtatatgaa caaccaaatt ttatgactaa ctgtgggtcg tgtacttttg acgattgagt 1201 catttttacc aattaggtaa ttgaatttta ctaatttgtc attaaccgcg ctggccatcc 1261 caacaacaac aacaacaatc aatttaaaaa cttgacaagg caaaaaaaaa aaatttcatc 1321 tttgaaaaat ttttcttcct tttcttcttc tgttctttac ataacagcta tcaagaataa 1381 tcaattaata atgtcacaac aacaagataa acaacctagt gaaaataaat tatggggtgg 1441 tcgtttcacc ggtgccactg atccattgat ggatttgtat aatgcttcat taccttatga 1501 taaagttatg tatgatgctg atttaactgg tactaaagta tacacacaag gattaaacaa 1561 attaggatta attaccactg aagaattgaa tttgattcat caaggattag aacaaatcag 1621 acaagaatgg catgataata aattcattat taaggctggt gatgaggata ttcatactgc 1681 taatgaacgt cgattaggag aaatcattgg gaaaaacatt tctggtaaag ttcacactgg 1741 tagatcaaga aatgatcaag ttgctaccga tatgagaatt ttcgttcgtg aatcattact 1801 taatttaagt aaaattttac atcaattcat cacggcaatt cttgaacgag cacataaaga 1861 aatcgatgta ttaatgcctg gttatactca tttacaaaaa gctcaaccta ttagatgggc 1921 ccattggtta agttcatatg caacttattt caccgaagat tataaacgtt tacaagaaat 1981 tataactcgt gttaaccaat ctccattagg atctggtgct ttagcaggtc atccttatgg 2041 tattgatcgt gaatttttag ctaaaggatt aggatttgat ggtgtcattg ggaattcttt 2101 aactgcagtt tcagatcgtg attttgttgt tgaaagttta ttttggagta cattatttat 2161 gaatcatatt tctcgattct cagaagatct aattatttat tctagtggtg aatttgggtt 2221 cataaaatta gctgatgctt attctactgg ttcttcattg atgccacaaa agaaaaaccc 2281 tgactcatta gaattattac gtgggaaaag tggtagagta tttggtcagt tatctggatt 2341 tttaatgtcg atcaaatcta ttccatctac ttataataaa gatatgcaag aggataaaga 2401 accattattt gatgcattga ctacagtaga acattcaatt ttaattgcta ctggtgtgat 2461 ttctacttta ctgattgata aacaaaatat ggaaaaagct ttaactatgg atatgttggc 2521 tactgattta gctgattatt tagttagaaa aggagttcca tttagagaaa ctcatcatat 2581 atctggtgaa tgtgttagaa aagctgaaga agaaaaatta tctggaattg atcaattatc 2641 ttttgaacaa tttcaacaaa ttgattctcg ttttgaaaaa gacgttatgg aaacttttga 2701 ttttgaagct agtgtggaaa gaagagatgc tcttggtggt actgctaaaa gtgccgtttt 2761 aaaacaatta gaaaatttaa aatcaatctt aagttaattt ataaatagtc atataataat 2821 cacagtattg tttaacttac ataaaatata atcaattaac acagagatac cttgtactac 2881 aatagtttct gatatcccag ttttcgttgg agaaggtaat tgtgacttgg gtataactgg 2941 gttagaccaa atcaaagaag ctgaacaatt cgacaacatc gaggacttgt tggatttgaa 3001 atttggttca tgcaaattgc agatccaagt tccagcagat ggcgagtacg aaaagccaga 3061 acagcttgtt ggaaagaaaa ttgtgtcttc atttacaaaa ttgagtaccg actatttcaa 3121 acaattgtca gacaaaccta ctaatatcag atatgtcggt ggttccgttg aggcttcttg 3181 tgccttgggt gttgctgatg ctattgtcga tttggttgaa agtggtgaaa ctatgaaagc 3241 agctgggtta aaggccatag aaaccatatt ggagacttcg gctcatttga tttcgtcaaa 3301 aaaaagtaaa ttcccagaaa tggtcaatat aatcgtccaa agacttcaag gtgttttagc 3361 tgcccaggaa tatgtcttgt gtaactacaa tgccccaaaa tccatccaag caaaatgttt 3421 aaccattact ccaggcagaa gggccgctac tgtctctact ttggacaaac acagcgacga 3481 cgaagaggac tgggttgcca tctcatccat ggttaataga aaggaaatcg gtaatgtaat 3541 ggacgaattg aagaaagctg gtgcaaccga tatattggta ctcgagatat caaattgtag 3601 agtttaaaag aagtgatagt ttctcataaa tattatacat atatacttat agatctactt 3661 caatttgact caatctgtct tataccatct ttatctacca ccttgatgta cacaccttta 3721 aagtcaatag gcattctcat aacttcgtat aatgtatgct atacgaacgg tagtttaaac 3781 gagctcggtt cagcaccttg tcgagtttac cactccctat cagtgataga gaaaagtgaa 3841 agtcgagttt accactccct atcagtgata gagaaaagtg aaagtcgagt ttaccactcc 3901 ctatcagtga tagagaaaag tgaaagtcga gtttaccact ccctatcagt gatagagaaa 3961 agtgaaagtc gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac 4021 cactccctat cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata 4081 gagaaaagtg aaagtcgagc tccaaacttc ttttttattt ttcgcaatat tttcgataaa 4141 ttaaatataa actactcgat tgagtaatag attcaataac tatgggaatt actaacaatg 4201 atttaaggaa aaagcttcac ttaaaaataa agccctttca attaaagtgt tgtccatgtt 4261 tgtcgtttct gatgggcttt tctgaaatat aaatatagat tcaaatctct tgtattcaag 4321 ttttagattt ttttttattt cttttatatc attatcaaat caaatcaaca aactctttca 4381 acttctcaga aagaaatcaa tatcaactac aataaaacca actacgtcta ctactacgtt 4441 aactcctaca cacatacaaa tataaatagt cgacaaagat g //