LOCUS       p416-HAH2-MET3          9973 bp    DNA     circular     15-MAY-2020
DEFINITION  LMBP 11190.
SOURCE      
  ORGANISM  
COMMENT     Accession number: LMBP 11190
            (c) BCCM/GeneCorner Plasmid Collection (Ghent University, Belgium)
            Last modified: 15-MAY-2020
            NGS data implemented
            
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     VNTDATE|-13342293|
COMMENT     VNTDBDATE|-13342310|
COMMENT     LSOWNER|
COMMENT     VNTNAME|p416-HAH2-MET3|
COMMENT     VNTAUTHORNAME|Demo User|
COMMENT     VNTREPLTYPE|Plasmid
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FEATURES             Location/Qualifiers
     rep_origin      4587..4989
                     /vntifkey="33"
                     /label=pMB1\ori
     promoter        5081..5164
                     /vntifkey="30"
                     /label=lac\promoter
     RBS             5166..5202
                     /vntifkey="32"
                     /label=5'\UTR\of\lacZ,\incl.\RBS
     promoter        5228..5244
                     /vntifkey="30"
                     /label=T3\promoter
     promoter        complement(494..510)
                     /vntifkey="30"
                     /label=T7\promoter
     rep_origin      674..1129
                     /vntifkey="33"
                     /label=f1\ori
     CDS             complement(1260..2063)
                     /vntifkey="4"
                     /label=URA3
     rep_origin      complement(2526..3050)
                     /vntifkey="33"
                     /label=CEN6/ARSH4\ori
     CDS             3186..4046
                     /vntifkey="4"
                     /label=ampicillin\resistance
     misc_feature    5839..6192
                     /vntifkey="21"
                     /label=HIS1
     misc_feature    8152..8505
                     /vntifkey="21"
                     /label=HIS1
     misc_feature    5286..5319
                     /vntifkey="21"
                     /label=LoxLE
     misc_feature    9002..9035
                     /vntifkey="21"
                     /label=LoxRE
     promoter        9044..468
                     /vntifkey="29"
                     /label=CaMET3\promoter
     CDS             6654..8060
                     /vntifkey="4"
                     /label=ARG4
BASE COUNT     2995 a      1943 c      1951 g      3084 t 
ORIGIN
        1 gaacgacata gatccattaa tgcgcccctc taaaacaata cccataatgt cttcttaagt 
       61 atgcatctgc ttataaatgt ttctggattt actttagtta gaatgtcgac acgacaatga 
      121 actattgctg tagatcacgt gcgatcactg aattatgagt ttctttttat ttttcttttt 
      181 gggctttttt tttttgcaat tttttttttc cacctttgcc aattgcgtag tcacatataa 
      241 tgtatacaat ttttgcaact ctgacactat aattgatcca ataataaagt atataataga 
      301 tcatcaagta tacgtaatct ccccaaaaaa aaaattgcat aaaataaaat ctataaacct 
      361 tttttagatt tttagttgtt ccttttttct ttctattctt caacttccaa gtgttgtcac 
      421 tttcttcctg ttaactatat ttaaaagtaa ataccctccc cagaaaacat gactagtggt 
      481 acccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg 
      541 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc 
      601 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct 
      661 gaatggcgaa tggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg 
      721 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct 
      781 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta 
      841 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt 
      901 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg 
      961 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat 
     1021 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt 
     1081 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaatttc ctgatgcggt 
     1141 attttctcct tacgcatctg tgcggtattt cacaccgcat agggtaataa ctgatataat 
     1201 taaattgaag ctctaatttg tgagtttagt atacatgcat ttacttataa tacagttttt 
     1261 tagttttgct ggccgcatct tctcaaatat gcttcccagc ctgcttttct gtaacgttca 
     1321 ccctctacct tagcatccct tccctttgca aatagtcctc ttccaacaat aataatgtca 
     1381 gatcctgtag agaccacatc atccacggtt ctatactgtt gacccaatgc gtctcccttg 
     1441 tcatctaaac ccacaccggg tgtcataatc aaccaatcgt aaccttcatc tcttccaccc 
     1501 atgtctcttt gagcaataaa gccgataaca aaatctttgt cgctcttcgc aatgtcaaca 
     1561 gtacccttag tatattctcc agtagatagg gagcccttgc atgacaattc tgctaacatc 
     1621 aaaaggcctc taggttcctt tgttacttct tctgccgcct gcttcaaacc gctaacaata 
     1681 cctgggccca ccacaccgtg tgcattcgta atgtctgccc attctgctat tctgtataca 
     1741 cccgcagagt actgcaattt gactgtatta ccaatgtcag caaattttct gtcttcgaag 
     1801 agtaaaaaat tgtacttggc ggataatgcc tttagcggct taactgtgcc ctccatggaa 
     1861 aaatcagtca agatatccac atgtgttttt agtaaacaaa ttttgggacc taatgcttca 
     1921 actaactcca gtaattcctt ggtggtacga acatccaatg aagcacacaa gtttgtttgc 
     1981 ttttcgtgca tgatattaaa tagcttggca gcaacaggac taggatgagt agcagcacgt 
     2041 tccttatatg tagctttcga catgatttat cttcgtttcc tgcaggtttt tgttctgtgc 
     2101 agttgggtta agaatactgg gcaatttcat gtttcttcaa cactacatat gcgtatatat 
     2161 accaatctaa gtctgtgctc cttccttcgt tcttccttct gttcggagat taccgaatca 
     2221 aaaaaatttc aaggaaaccg aaatcaaaaa aaagaataaa aaaaaaatga tgaattgaaa 
     2281 aggtggtatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc 
     2341 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt 
     2401 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac 
     2461 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga 
     2521 taataatggt ttcttagacg gatcgcttgc ctgtaactta cacgcgcctc gtatctttta 
     2581 atgatggaat aatttgggaa tttactctgt gtttatttat ttttatgttt tgtatttgga 
     2641 ttttagaaag taaataaaga aggtagaaga gttacggaat gaagaaaaaa aaataaacaa 
     2701 aggtttaaaa aatttcaaca aaaagcgtac tttacatata tatttattag acaagaaaag 
     2761 cagattaaat agatatacat tcgattaacg ataagtaaaa tgtaaaatca caggattttc 
     2821 gtgtgtggtc ttctacacag acaagatgaa acaattcggc attaatacct gagagcagga 
     2881 agagcaagat aaaaggtagt atttgttggc gatcccccta gagtctttta catcttcgga 
     2941 aaacaaaaac tattttttct ttaatttctt tttttacttt ctatttttaa tttatatatt 
     3001 tatattaaaa aatttaaatt ataattattt ttatagcacg tgatgaaaag gacccaggtg 
     3061 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa 
     3121 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga 
     3181 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc 
     3241 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg 
     3301 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc 
     3361 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat 
     3421 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg 
     3481 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag 
     3541 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa 
     3601 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc 
     3661 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca 
     3721 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc 
     3781 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc 
     3841 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg 
     3901 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta 
     3961 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag 
     4021 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga 
     4081 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc 
     4141 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa 
     4201 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa 
     4261 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc 
     4321 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt 
     4381 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc 
     4441 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac 
     4501 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca 
     4561 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg 
     4621 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag 
     4681 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt 
     4741 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat 
     4801 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc 
     4861 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt 
     4921 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag 
     4981 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca 
     5041 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga 
     5101 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt 
     5161 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca 
     5221 agctcgaaat taaccctcac taaagggaac aaaagctgga gctccttcgt acgctgcagg 
     5281 tcgactaccg ttcgtataat gtatgctata cgaagttatg gatcctggag gatgaggaga 
     5341 cagaagttag tagtaacaat ttggggacag aagaggatac agtgacatca gaggaagaag 
     5401 acagcgaagg tatagtcaaa aagcaatagg acagcaagac aatttattta tttaatagtt 
     5461 ttttataaat gtaaaatcta tcacaacatg tgtacttaat tttctgtggt aggggaggcc 
     5521 atttcgttga aacgtgtcgc gagcggttgt gagaattgac tcattttgat tattacgcaa 
     5581 ccattgaaat tttttatttt tttttggtga agatttttcc cacacaactt cttcttttac 
     5641 ttaaccacca actaccgata atggatttag tcaatcattt accagaccgt ttgttatttg 
     5701 ctgttcctaa aaagggcaga ttatacgaaa aatgctgtaa cttattgagt ggtgccgata 
     5761 tacagtttag aagatctaat agattagata tagcactttc tacaaacttg ccaattgcat 
     5821 taatcttctt gcctgcagct gatatcccag ttttcgttgg agaaggtaat tgtgacttgg 
     5881 gtataactgg gttagaccaa atcaaagaag ctgaacaatt cgacaacatc gaggacttgt 
     5941 tggatttgaa atttggttca tgcaaattgc agatccaagt tccagcagat ggcgagtacg 
     6001 aaaagccaga acagcttgtt ggaaagaaaa ttgtgtcttc atttacaaaa ttgagtaccg 
     6061 actatttcaa acaattgtca gacaaaccta ctaatatcag atatgtcggt ggttccgttg 
     6121 aggcttcttg tgccttgggt gttgctgatg ctattgtcga tttggttgaa agtggtgaaa 
     6181 ctatgaaagc aggatccccc ctttagtaag atttttcaag agtagtctca aataaaccag 
     6241 aatgaaggat tgaaggtttt attcaataaa taattggaca gaaagttatt gtacagtttt 
     6301 aacaacaaca atttcaaaat ttcaatttat gacatattga ccgacataat tcagtcattt 
     6361 ctaatattca aataacaaga caccttgtat aaatgaatcc acaatcgtat atgaacaacc 
     6421 aaattttatg actaactttg ggtcgtgtac ttttgacgat tgagtcattt ttaccaatta 
     6481 ggtaattgaa ttttgttaat ttgtcattaa ccgcgctggc catcccaaca ataacaacaa 
     6541 tcaatttaaa aacttgacaa ggcaaaaaaa aaaaaatttt atctttgaaa aatttttctt 
     6601 ccttttcttc ttctgttctt tacataacag ctatcaagaa taatcaatta ataatgtcac 
     6661 aacaacaaga taaacaacct agtgaaaata aattatgggg tggtcgtttc accggtgcca 
     6721 ctgatccatt gatggattta tataatgctt cattacctta tgataaagtt atgtatgatg 
     6781 ctgatttaac tggtactaaa gtatacacac aaggattaaa caaattagga ttaattacca 
     6841 ctgaagaatt gcatttgatt catcaaggat tagaacaaat cagacaagaa tggcatgata 
     6901 ataaattcat tattaaggca ggtgatgagg atattcatac tgctaatgaa cgtcgattag 
     6961 gagaaatcat tgggaaaaac atttctggta aagttcacac tggtagatca agaaatgatc 
     7021 aagttgctac cgatatgaga attttcgttc gtgaatcatt acttaattta agtaaaattt 
     7081 tacatcaatt catcacggca attcttgaac gggcacataa agaaatcgat gtattaatgc 
     7141 ctggttatac tcatttacaa aaagctcaac ctattagatg ggcacattgg ttaagttcat 
     7201 atgcaactta tttcaccgaa gattataaac gtttacaaga aattataact cgtgttaacc 
     7261 aatctccatt aggatctggt gctttagcag gtcatcctta tggtattgat cgtgaatttt 
     7321 tagctaaagg attaggattt gatggtgtca ttgggaattc tttaactgca gtttcagatc 
     7381 gtgattttgt tgttgaaagt ttattttgga gtacattatt tatgaatcat atttctcgat 
     7441 tctcagaaga tctaattatt tattctagtg gtgaatttgg gttcataaaa ttagctgatg 
     7501 cttattctac tggttcttca ttgatgccac aaaagaaaaa ccctgactca ttagaattat 
     7561 tacgtgggaa aagtggtaga gtatttggtc aattatctgg atttttaatg tcgatcaaat 
     7621 ctattccatc tacttataat aaagatatgc aagaggataa agaaccatta tttgatgcat 
     7681 tgactacagt ggaacattca attttaattg ctactggtgt gatttctact ttactgattg 
     7741 ataaacaaaa tatggaaaaa gctttaacta tggatatgtt ggctactgat ttagctgatt 
     7801 atttagttag aaaaggagtt ccatttagag aaactcatca tatatctggt gaatgtgtta 
     7861 gaaaagctga agaagaaaaa ttatctggaa ttgatcaatt atcttttgaa caatttcaac 
     7921 aaattgattc tcgttttgaa aaagacgtta tggaaacttt tgattttgaa gctagtgtgg 
     7981 aaagaagaga tgctattggt ggtactgcta aaagtgccgt tttaaaacaa ttagaaaatt 
     8041 taaaatcaat tttaagttaa tttataaata gtcatataat aatcacagta ttgtttaact 
     8101 tacataaaat ataatcaatt aacacagaga taccttgtac tacaatagtt tctgatatcc 
     8161 cagttttcgt tggagaaggt aattgtgact tgggtataac tgggttagac caaatcaaag 
     8221 aagctgaaca attcgacaac atcgaggact tgttggattt gaaatttggt tcatgcaaat 
     8281 tgcagatcca agttccagca gatggcgagt acgaaaagcc agaacagctt gttggaaaga 
     8341 aaattgtgtc ttcatttaca aaattgagta ccgactattt caaacaattg tcagacaaac 
     8401 ctactaatat cagatatgtc ggtggttccg ttgaggcttc ttgtgccttg ggtgttgctg 
     8461 atgctattgt cgatttggtt gaaagtggtg aaactatgaa agcagctggg ttaaaggcca 
     8521 tagaaaccat attggagact tcggctcatt tgatttcgtc aaaaaaaagt aaattcccag 
     8581 aaatggtcaa tataatcgtc caaagacttc aaggtgtttt agctgcccag gaatatgtct 
     8641 tgtgtaacta caatgcccca aaatccatcc aagcaaaatg tttaaccatt actccaggca 
     8701 gaagggccgc tactgtctct actttggaca aacacagcga cgacgaagag gactgggttg 
     8761 ccatctcatc catggttaat agaaaggaaa tcggtaatgt aatggacgaa ttgaagaaag 
     8821 ctggtgcaac cgatatattg gtactcgaga tatcaaattg tagagtttaa aagaagtgat 
     8881 agtttctcat aaatattata catatatact tatagatcta cttcaatttg actcaatctg 
     8941 tcttatacca tctttatcta ccaccttgat gtacacacct ttaaagtcaa taggcattct 
     9001 cataacttcg tataatgtat gctatacgaa cggtagttta aacaatgact attggtagcg 
     9061 tgttctattt ttcttatgcg ctgaaaaact acgaacaatt gtctattcca agcctgtggc 
     9121 tttttttggt gattcacaat acataaattg gcaaatacct cttctataga ctttaacctt 
     9181 tgatgaaatg gtatggataa accacttttg tggtcagggt atatatattt ttattccaat 
     9241 ctggccttta taaattcaaa tttccaaggg gactctgcta aaccttctga tataactata 
     9301 actctaataa tggctgtaaa aagtgtgaaa aaaagatgat attcacaaaa aagataaagg 
     9361 ggatttcata ttgggtatct agatttaaac atcgtatttc aacaatgaaa ctagaatcaa 
     9421 tacgaaatcc agggcacaac tgtgttcatt cccttgggaa ttatactgag taatgggaag 
     9481 aatgtgcaaa aaatacccgt gtcttatata atttccattt gtgtagggct taaaagtgga 
     9541 caacttcagg ttacgaaacc acgaaaatgt ctaaacgttt gatcaaattc gtaactcatg 
     9601 gaaaatcaca gcaacaaatt aaggatggat atatccggta cttacaaata aaaacactaa 
     9661 agaagtataa attttgaact gaaattctat agaaaatctt tttttttggg taaaattatg 
     9721 cgattgtggc tcatagtaac gtaatcatac agttcaacaa acatcatttt gtcaaaggat 
     9781 ttctaatcct attctttttc tacttggctt ttgagtatat atatatatac atcctttgtt 
     9841 catcatcatg ttcgattgaa tttttttctt ggttgttatt agattgtcta cttgagtttt 
     9901 tcttggtgca aattgtggtg tttttctaac aaatttttca acatttaaag tatttctgga 
     9961 acttttctac aag 
//
