LOCUS p416-SHS-TIM 8328 bp DNA linear SYN 30-MAY-2017 DEFINITION LMBP 11196. ACCESSION MF048871 VERSION MF048871.1 KEYWORDS . SOURCE synthetic construct (code 12) ORGANISM synthetic construct (code 12) other sequences; artificial sequences. REFERENCE 1 (bases 1 to 8328) AUTHORS Bijlani,S. and Ganesan,K. TITLE A Cassette for One-Step Gene Deletion and Regulated Gene Expression in Clinical Isolates of Diploid Candida species JOURNAL Unpublished REFERENCE 2 (bases 1 to 8328) AUTHORS Bijlani,S. and Ganesan,K. TITLE Direct Submission JOURNAL Submitted (04-MAY-2017) Yeast Molecular Biology, CSIR- Institute of Microbial Technology, Sector 39-A, Chandigarh, Chandigarh 160036, India COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..8328 /organism="synthetic construct (code 12)" /mol_type="other DNA" /db_xref="taxon:2005482" /clone="p416-SHS-TIM" misc_feature 1..34 /note="FLP recombination target sequence FRT" regulatory 39..586 /regulatory_class="promoter" /note="CaMAL2 promoter; derived from Candida albicans" CDS join(593..858,1023..2028) /note="FLP containing CaTUB2 intron" /codon_start=1 /transl_table=12 /product="site-specific recombinase" /protein_id="ART66091.1" /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWM ITHNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEF TIIPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEK ILNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCL VTETKTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLK DNLVRSYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKR ASAVARTTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKG SAEGSIRYPAWNGIISQEVLDYLSSYINRRI" regulatory 2029..2416 /regulatory_class="terminator" /note="CaACT1 terminator; derived from Candida albicans" regulatory 2423..2920 /regulatory_class="promoter" /note="CaACT1 promoter; derived from Candida albicans" gene 2921..3995 /gene="SAT1" /note="5' region; derived from C. albicans codon-optimized SAT1 gene" repeat_region 3682..3995 /gene="SAT1" /rpt_type=direct gene 4002..6260 /gene="HygB" /note="codon-optimized for C. albicans" regulatory 4002..4642 /regulatory_class="promoter" /gene="HygB" /note="CaTEF2 promoter; derived from C. albicans" CDS 4649..5674 /gene="HygB" /codon_start=1 /transl_table=12 /product="hygromycin resistance marker" /protein_id="ART66092.1" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS TRPRAKE" regulatory 5681..6260 /regulatory_class="terminator" /gene="HygB" /note="CaPFK2 terminator; derived from C. albicans" gene 6261..6726 /gene="SAT1" /note="3' region; derived from C. albicans codon-optimized SAT1 gene" repeat_region 6261..6574 /gene="SAT1" /rpt_type=direct misc_feature 6864..6897 /note="FLP recombination target sequence FRT" regulatory 6926..8325 /regulatory_class="promoter" /note="CaMET3 promoter; derived from Candida albicans" misc_feature 8326..8328 /note="Start codon" ORIGIN 1 gaagttccta tactttctag agaataggaa cttcggatcc aataatgatt ggtttgatat 61 ttttgtctag taccatctgt accattacac ttaaattatc tttatatctg tctaactcga 121 ctgtctggat ttcattgatg tagtcgtatg catcgttagt tccaaaaaat attgtcatca 181 atttgatatt ggtttccgac tctaaaattt ttggaagaat ttgtctagcg tgctctgagt 241 tgtagccact gaaaccacgg ttaataacat ccaattttcg gatatacaca ttctgtaatg 301 ctggatgaaa gccatactgg gtacaactaa actgggtgat ggagtcaccg aacaacacaa 361 atttaccgta ttccatgatt gctatggttg agaatttttt ttttttcttg tcccacgcca 421 tttttcaaat tatgcagttg agaatgttag tttttgtgta caccccgttc gctgaatatt 481 tcggaataat tcaaagattg gggagtgggg gaggcgatag acgaagacac ggtataaaaa 541 tgggcaaaat tttccccaac tttttgcagt ggtttaacta ataatcgtcg acatgccaca 601 atttgatata ttatgtaaaa caccacctaa ggtgcttgtt cgtcagtttg tggaaaggtt 661 tgaaagacct tcaggtgaga aaatagcatt atgtgctgct gaactaacct atttatgttg 721 gatgattaca cataacggaa cagcaatcaa gagagccaca ttcatgagct ataatactat 781 cataagcaat tcgttgagtt tcgatattgt caataaatca ctccagttta aatacaagac 841 gcaaaaagca acaattttgt aggtatagac actgaagaaa aaaaaattct atcattgtta 901 tgttgtgatc tttgatcttt agttgtcggg ttaacacctg ccaattggat caatacatca 961 atcaattaat tctaatcttg aaaaaaaaat tcattttact aacaattttt tcttttattt 1021 agggaagcct cattaaagaa attgattcct gcttgggaat ttacaattat tccttactat 1081 ggacaaaaac atcaatctga tatcactgat attgtaagta gtttgcaatt acagttcgaa 1141 tcatcggaag aagcagataa gggaaatagc cacagtaaaa aaatgcttaa agcacttcta 1201 agtgagggtg aaagcatctg ggagatcact gagaaaatac taaattcgtt tgagtatact 1261 tcgagattta caaaaacaaa aactttatac caattcctct tcctagctac tttcatcaat 1321 tgtggaagat tcagcgatat taagaacgtt gatccgaaat catttaaatt agtccaaaat 1381 aagtatttgg gagtaataat ccagtgttta gtgacagaga caaagacaag cgttagtagg 1441 cacatatact tctttagcgc aaggggtagg atcgatccac ttgtatattt ggatgaattt 1501 ttgaggaatt ctgaaccagt cctaaaacga gtaaatagga ccggcaattc ttcaagcaat 1561 aaacaggaat accaattatt aaaagataac ttagtcagat cgtacaataa agctttgaag 1621 aaaaatgcgc cttattcaat ctttgctata aaaaatggcc caaaatctca cattggaaga 1681 catttgatga cctcatttct ttcaatgaag ggcctaacgg agttgactaa tgttgtggga 1741 aattggagcg ataagcgtgc ttctgccgtg gccaggacaa cgtatactca tcagataaca 1801 gcaatacctg atcactactt cgcactagtt tctcggtact atgcatatga tccaatatca 1861 aaggaaatga tagcattgaa ggatgagact aatccaattg aggagtggca gcatatagaa 1921 cagctaaagg gtagtgctga aggaagcata cgataccccg catggaatgg gataatatca 1981 caggaggtac tagactacct ttcatcctac ataaatagac gcatataaga gtgaaattct 2041 ggaaatctgg aaatctggtt ttgtattctt gttattcttc tttttgttat tacatatata 2101 acttgttact tttttaaaaa aatctttgta tattttataa atatataaaa ctaaatttaa 2161 gaaaaagaga aaaatgtttt atttgagaga ttgaaatttt acttgaattt agcttagctt 2221 ttataaagta ttattatgta aaaaaacaaa acaaatatac attaaaaagt taagactata 2281 aaatagccac ccaaggcatt tctatatctt gttgttgttg ttttcatctt ctgtatcaga 2341 ggaacttatt ttattatttt cgtcacgggt attttctctt gtttgatgat tcatcccatt 2401 cattccatca taaaatgtcg agcgtcaaaa ctagagaata ataaagaaaa cgatcttttc 2461 aaaaagaaaa aaccttttag ttttcctttg ttgttgttgt gggtgtgtgc tatttatatt 2521 atatagttta ctcataatac cataaaatat tcggtttgat taggttattt taataagcta 2581 atttgtttct aatcgtgtaa tttatgctgt gtatattaag tagtgtgtgc actgcccaaa 2641 aatgtttgtt gtttatagtc ggttaaagag aaaaaagaaa aaaagatcca tacacacacg 2701 ttaattagtt gttcaacgta atacactcat attttgttct tatttgcttt cggtcgctgt 2761 tctcaccaag atttattgcc aacgaaacaa ttttttttta tatattttca gatttttctt 2821 tttttccttt cctttccttt tctaattttc actcctggtt ttctttcttt cttagaaaca 2881 ttatctcgat attaatatta aaaaaatata atcattcaaa atggacggtg gtatgtttta 2941 gtttagcttc aattctaatt gattgattaa tcagttgatt ggtttcaata tgacaaatgg 3001 gtagggtggg aaaacttcat tttcaattca gatcaaactt ttttgttgtc gacataatat 3061 ttctcgtttg ggatgttact gtcacattaa taatacacac acatcagctt ataattttga 3121 aagtaattta tcagatatgt tgtgacgatc aatggaaatg gctaacttca atgtatctgt 3181 tcttcccctt tttcaaagtt cacgtttttt gattgattga ttgatctgtc ggcagtggtt 3241 tcaaaaccat tcggtgagta atcctatcaa tcaatgttac gacaaaaggc tcaatattca 3301 aaattgcaat gttttatgtt ttcctacgtg tacttgtgca aggcaattga ttcaacattg 3361 cttttggtgt ttgacgagtt tctagtttgg acttgtgttg ttatctgggc tatacagatt 3421 tcccggctca ctatgaattt tttttttcga cgctcagtgc acacaactat aaacaacaca 3481 aacacaaaca cagcaagaaa aaaaaaaaac gaacattgaa ttgaaaccaa gccaactgaa 3541 aaattcctta tttaaatgac tgtcatacta acccattttt atagaagaag ttgctgcttt 3601 agttatcgat aacggttctc atatgaaaat ttcggtgatc cctgagcagg tggcggaaac 3661 attggatgct gagaaccatt tcattgttcg tgaagtgttc gatgtgcacc tatccgacca 3721 aggctttgaa ctatctacca gaagtgtgag cccctaccgg aaggattaca tctcggatga 3781 tgactctgat gaagactctg cttgctatgg cgcattcatc gaccaagagc ttgtcgggaa 3841 gattgaactc aactcaacat ggaacgatct agcctctatc gaacacattg ttgtgtcgca 3901 cacgcaccga ggcaaaggag tcgcgcacag tctcatcgaa tttgcgaaaa agtgggcact 3961 aagcagacag ctccttggca tacgattaga gacacgacgt cgtatagtgc ttgctgttcg 4021 atattgctag aggcaaagaa aaaggattac ctcacgccta aaatggaatt tgaaaattga 4081 ggagatgagt tgagcttttg tcttttacac tttccctatt caattgtgcc gcaaaaactt 4141 caatatctaa cacagtgtga aatatttgtt atgtataccg aaagccaaat accatttaaa 4201 ctggtatttc aagatttcat aaatcaagtt tctggttaaa ttaattgctg ttaaacagaa 4261 caagagcttc acattgatgt tggaataact actatagaat tataaaatac acaaaaccag 4321 gtcgtgtacg caataacttt tcaatgtatg aaaaaatttt tttttttttt ttgcttcttc 4381 tcttcgcttc ttttttctag ttatcaattt ggcaatgaat tgatttgatt gccctttgtt 4441 tagtttacta tgaaaaaaaa tttttttttt gtctctgtca gattttctct catttttttt 4501 tttgcctcat aatgaacaac tcaaaaaaga atataaattt ctcatcaaag acctttccaa 4561 ttgaaaattt tttctttcct tcttttcctt tttttttttc tttctttctt cttttctaag 4621 tatacatttt atataatcaa tcctcgagat gaaaaaacca gaattgactg ctacttctgt 4681 tgaaaaattt ttgattgaaa aatttgattc tgtttctgat ttgatgcaat tgtctgaagg 4741 tgaagaatca agagcttttt cttttgatgt tggtggtaga ggttatgttt tgagagttaa 4801 ttcttgtgct gatggttttt ataaagatag atatgtttat agacattttg cttctgctgc 4861 tttgccaatt ccagaagttt tggatattgg tgaattttct gaatctttga cttattgtat 4921 ttcaagaaga gctcaaggtg ttactttgca agatttgcca gaaactgaat tgccagctgt 4981 tttgcaacca gttgctgaag ctatggatgc tattgctgct gctgatttgt ctcaaacttc 5041 tggttttggt ccatttggtc cacaaggtat tggtcaatat actacttgga gagattttat 5101 ttgtgctatt gctgatccac atgtttatca ttggcaaact gttatggatg atactgtttc 5161 tgcttctgtt gctcaagcat tggatgaatt gatgttgtgg gctgaagatt gtccagaagt 5221 tagacatttg gttcatgctg attttggttc taataatgtt ttgactgata atggtagaat 5281 tactgctgtt attgattggt ctgaagctat gtttggtgat tctcaatatg aagttgctaa 5341 tatttttttt tggagaccat ggttggcttg tatggaacaa caaactagat attttgaaag 5401 aagacatcca gaattggctg gttctccaag attgagagct tatatgttga gaattggttt 5461 ggatcaattg tatcaatctt tggttgatgg taattttgat gatgctgctt gggctcaagg 5521 tagatgtgat gctattgtta gatcaggtgc tggtactgtt ggtagaactc aaattgctag 5581 aagatcagct gctgtttgga ctgatggttg tgttgaagtt ttggctgatt ctggtaatag 5641 aagaccatct actagaccaa gagctaaaga ataaggatcc gacgaagttg gtgtatgata 5701 acgaatttat agtttttata ttgtaaaata atgaaatatt tgtttttttt ttactttgat 5761 gtatatgctg tatcccaatc ctcgatacat tgcagaattg aatcatttgg tttatctgtt 5821 gttttgtgct aagtaagata ttttccatct agaagtgata gctttttatt atcaaattac 5881 aatccctatt acatgtggac atcaactcca tttctttgcg cattttatag tagagttaga 5941 gttagcttgt cacatctcgt tgcttccttt tgctgcaact gtttccgatt tcaaaatgaa 6001 aatagcttaa agttacccgg agggcctgca cataatcaaa atatagtaaa ttcccaaaag 6061 aacagtcgtg tgcagtttcc aaagtgaaat tttttttttg tttctctcac ttccttgtaa 6121 ggttgtttcc ctcttctttc tctctaacca atcctttgta gattgaaaat tttttcttgc 6181 aaatttttct ttaactctct ttcccttttt ttttcgaatc aaggtaccat atcattacca 6241 aattagacat catgcctatg cattgttcgt gaagtgttcg atgtgcacct atccgaccaa 6301 ggctttgaac tatctaccag aagtgtgagc ccctaccgga aggattacat ctcggatgat 6361 gactctgatg aagactctgc ttgctatggc gcattcatcg accaagagct tgtcgggaag 6421 attgaactca actcaacatg gaacgatcta gcctctatcg aacacattgt tgtgtcgcac 6481 acgcaccgag gcaaaggagt cgcgcacagt ctcatcgaat ttgcgaaaaa gtgggcacta 6541 agcagacagc tccttggcat acgattagag acacaaacga acaatgtacc tgcctgcaat 6601 ttgtacgcaa aatgtggctt tactctcggc ggcattgacc tcttcacgta taaaactaga 6661 cctcaagtct cgaacgaaac agcgatgtac tggtactggt tctcgggagc acaggatgac 6721 gcctaacata tgtgaagtgt gaagggggag attttcactt tattagattt gtatatatgt 6781 ataataaata aataaataag ttaaataaat aattagataa gggtggtaat tattactatt 6841 tacaatcaaa ggtggtcctg caggaagttc ctatactttc tagagaatag gaacttcaga 6901 tccactagtt ctagagcggt ttaaacaatg actattggta gcgtgttcta tttttcttat 6961 gcgctgaaaa actacgaaca attgtctatt ccaagcctgt ggcttttttt ggtgattcac 7021 aatacataaa ttggcaaata cctcttctat agactttaac ctttgatgaa atggtatgga 7081 taaaccactt ttgtggtcag ggtatatata tttttattcc aatctggcct ttataaattc 7141 aaatttccaa ggggactctg ctaaaccttc tgatataact ataactctaa taatggctgt 7201 aaaaagtgtg aaaaaaagat gatattcaca aaaaagataa aggggatttc atattgggta 7261 tctagattta aacatcgtat ttcaacaatg aaactagaat caatacgaaa tccagggcac 7321 aactgtgttc attcccttgg gaattatact gagtaatggg aagaatgtgc aaaaaatacc 7381 cgtgtcttat ataatttcca tttgtgtagg gcttaaaagt ggacaacttc aggttacgaa 7441 accacgaaaa tgtctaaacg tttgatcaaa ttcgtaactc atggaaaatc acagcaacaa 7501 attaaggatg gatatatccg gtacttacaa ataaaaacac taaagaagta taaattttga 7561 actgaaattc tatagaaaat cttttttttt tgggtaaaat tatgcgattg tggctcatag 7621 taacgtaatc atacagttca acaaacatca ttttgtcaaa ggatttctaa tcctattctt 7681 tttctacttg gcttttgagt atatatatat atacatcctt tgttcatcat catgttcgat 7741 tgaatttttt tcttggttgt tattagattg tctacttgag tttttcttgg tgcaaattgt 7801 ggtgtttttc taacaaattt ttcaacattt aaagtatttc tggaactttt ctacaaggaa 7861 cgacatagat ccattaatgc gcccctctaa aacaataccc ataatgtctt cttaagtatg 7921 catctgctta taaatgtttc tggatttact ttagttagaa tgtcgacacg acaatgaact 7981 attgctgtag atcacgtgcg atcactgaat tatgagtttc tttttatttt tctttttggg 8041 cttttttttt ttgcaatttt ttttttccac ctttgccaat tgcgtagtca catataatgt 8101 atacaatttt tgcaactctg acactataat tgatccaata ataaagtata taatagatca 8161 tcaagtatac gtaatctccc caaaaaaaaa attgcataaa ataaaatcta taaacctttt 8221 ttagattttt agttgttcct tttttctttc tattcttcaa cttccaagtg ttgtcacttt 8281 cttcctgtta actatattta aaagtaaata ccctccccag aaaacatg //