LOCUS p416-CtSHS-M 8257 bp DNA linear SYN 30-MAY-2017 DEFINITION LMBP 11198. ACCESSION MF048873 VERSION MF048873.1 KEYWORDS . SOURCE synthetic construct (code 12) ORGANISM synthetic construct (code 12) other sequences; artificial sequences. REFERENCE 1 (bases 1 to 8257) AUTHORS Bijlani,S. and Ganesan,K. TITLE A Cassette for One-Step Gene Deletion and Regulated Gene Expression in Clinical Isolates of Diploid Candida species JOURNAL Unpublished REFERENCE 2 (bases 1 to 8257) AUTHORS Bijlani,S. and Ganesan,K. TITLE Direct Submission JOURNAL Submitted (04-MAY-2017) Yeast Molecular Biology, CSIR- Institute of Microbial Technology, Sector 39-A, Chandigarh, Chandigarh 160036, India COMMENT ##Assembly-Data-START## Sequencing Technology :: Sanger dideoxy sequencing ##Assembly-Data-END## FEATURES Location/Qualifiers source 1..8257 /organism="synthetic construct (code 12)" /mol_type="other DNA" /db_xref="taxon:2005482" /clone="p416-CtSHS-M" misc_feature 1..34 /note="FLP recombination target sequence FRT" regulatory 39..679 /regulatory_class="promoter" /note="CtMAL2 promoter; derived from Candida tropicalis" gene 686..1957 /gene="FLP" CDS 686..1957 /gene="FLP" /codon_start=1 /transl_table=12 /product="site-specific recombinase" /protein_id="ART66095.1" /translation="MPQFDILCKTPPKVLVRQFVERFERPSGEKIALCAAELTYLCWM ITHNGTAIKRATFMSYNTIISNSLSFDIVNKSLQFKYKTQKATILEASLKKLIPAWEF TIIPYYGQKHQSDITDIVSSLQLQFESSEEADKGNSHSKKMLKALLSEGESIWEITEK ILNSFEYTSRFTKTKTLYQFLFLATFINCGRFSDIKNVDPKSFKLVQNKYLGVIIQCL VTETKTSVSRHIYFFSARGRIDPLVYLDEFLRNSEPVLKRVNRTGNSSSNKQEYQLLK DNLVRSYNKALKKNAPYSIFAIKNGPKSHIGRHLMTSFLSMKGLTELTNVVGNWSDKR ASAVARTTYTHQITAIPDHYFALVSRYYAYDPISKEMIALKDETNPIEEWQHIEQLKG SAEGSIRYPAWNGIISQEVLDYLSSYINRRI" regulatory 1958..2345 /regulatory_class="terminator" /note="CaACT1 terminator; derived from Candida albicans" regulatory 2352..2849 /regulatory_class="promoter" /note="CaACT1 promoter; derived from Candida albicans" gene 2850..3924 /gene="SAT1" /note="5' region; derived from C. albicans codon-optimized SAT1 gene" repeat_region 3611..3924 /gene="SAT1" /rpt_type=direct gene 3931..6189 /gene="HygB" /note="codon-optimized for C. albicans" regulatory 3931..4571 /regulatory_class="promoter" /gene="HygB" /note="CaTEF2 promoter; derived from C. albicans" CDS 4578..5603 /gene="HygB" /codon_start=1 /transl_table=12 /product="hygromycin resistance marker" /protein_id="ART66096.1" /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGR GYVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQD LPETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVY HWQTVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWS EAMFGDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQ SLVDGNFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPS TRPRAKE" regulatory 5610..6189 /regulatory_class="terminator" /gene="HygB" /note="CaPFK2 terminator; derived from C. albicans" gene 6190..6655 /gene="SAT1" /note="3' region; derived from C. albicans codon-optimized SAT1 gene" repeat_region 6190..6503 /gene="SAT1" /rpt_type=direct misc_feature 6793..6826 /note="FLP recombination target sequence FRT" regulatory 6856..8254 /regulatory_class="promoter" /note="CaMET3 promoter; derived from Candida albicans" misc_feature 8255..8257 /note="Start codon" ORIGIN 1 gaagttccta tactttctag agaataggaa cttcggatgg tagtttatag agaatataca 61 aacaattggt gatttatatt tataaaagtt aaaagccctg tataccgaat tactaagaaa 121 ttattgtctg atttgtaatt cagaacgcaa agtgtaatgc cctgtatctt attttttttt 181 ttttgcagct atacgataag aatctcattt ttttggtgcc aatgcgatcg tttatacgca 241 attcctaatt gaaacgtctc aacaaaaaaa ttgtaaatca tcttcaattg gtagctttaa 301 taactctaaa ttcaaggggg ggattgccag cgtatctgta taaacgagtg ttcttttcta 361 ctacattgct gtggatacta ttaatagaca aacagtagtg tgtgtataca catacagaat 421 ccatctctct ccgccacatt gtttccaata aaaaaatatt atccagtaat tggtgttttt 481 gtgtttctat ggatcaattc tcggaaaaat tatcgcgaga acaaagagca agtgtgggga 541 gatggggagt caacacagca agagaacaaa agctttaaat agtccatctt ttcaacggac 601 aagctgaaaa aaaagagcaa ttacttgtta ctcttcaatt ttttttttct ctacttttta 661 tatattcttc aaacaaaacg tcgacatgcc acaatttgat atattatgta aaacaccacc 721 taaggtgctt gttcgtcagt ttgtggaaag gtttgaaaga ccttcaggtg agaaaatagc 781 attatgtgct gctgaactaa cctatttatg ttggatgatt acacataacg gaacagcaat 841 caagagagcc acattcatga gctataatac tatcataagc aattcgttga gtttcgatat 901 tgtcaataaa tcactccagt ttaaatacaa gacgcaaaaa gcaacaattt tggaagcctc 961 attaaagaaa ttgattcctg cttgggaatt tacaattatt ccttactatg gacaaaaaca 1021 tcaatctgat atcactgata ttgtaagtag tttgcaatta cagttcgaat catcggaaga 1081 agcagataag ggaaatagcc acagtaaaaa aatgcttaaa gcacttctaa gtgagggtga 1141 aagcatctgg gagatcactg agaaaatact aaattcgttt gagtatactt cgagatttac 1201 aaaaacaaaa actttatacc aattcctctt cctagctact ttcatcaatt gtggaagatt 1261 cagcgatatt aagaacgttg atccgaaatc atttaaatta gtccaaaata agtatttggg 1321 agtaataatc cagtgtttag tgacagagac aaagacaagc gttagtaggc acatatactt 1381 ctttagcgca aggggtagga tcgatccact tgtatatttg gatgaatttt tgaggaattc 1441 tgaaccagtc ctaaaacgag taaataggac cggcaattct tcaagcaata aacaggaata 1501 ccaattatta aaagataact tagtcagatc gtacaataaa gctttgaaga aaaatgcgcc 1561 ttattcaatc tttgctataa aaaatggccc aaaatctcac attggaagac atttgatgac 1621 ctcatttctt tcaatgaagg gcctaacgga gttgactaat gttgtgggaa attggagcga 1681 taagcgtgct tctgccgtgg ccaggacaac gtatactcat cagataacag caatacctga 1741 tcactacttc gcactagttt ctcggtacta tgcatatgat ccaatatcaa aggaaatgat 1801 agcattgaag gatgagacta atccaattga ggagtggcag catatagaac agctaaaggg 1861 tagtgctgaa ggaagcatac gataccccgc atggaatggg ataatatcac aggaggtact 1921 agactacctt tcatcctaca taaatagacg catataagag tgaaattctg gaaatctgga 1981 aatctggttt tgtattcttg ttattcttct ttttgttatt acatatataa cttgttactt 2041 ttttaaaaaa atctttgtat attttataaa tatataaaac taaatttaag aaaaagagaa 2101 aaatgtttta tttgagagat tgaaatttta cttgaattta gcttagcttt tataaagtat 2161 tattatgtaa aaaaacaaaa caaatataca ttaaaaagtt aagactataa aatagccacc 2221 caaggcattt ctatatcttg ttgttgttgt tttcatcttc tgtatcagag gaacttattt 2281 tattattttc gtcacgggta ttttctcttg tttgatgatt catcccattc attccatcat 2341 aaaatgtcga gcgtcaaaac tagagaataa taaagaaaac gatcttttca aaaagaaaaa 2401 accttttagt tttcctttgt tgttgttgtg ggtgtgtgct atttatatta tatagtttac 2461 tcataatacc ataaaatatt cggtttgatt aggttatttt aataagctaa tttgtttcta 2521 atcgtgtaat ttatgctgtg tatattaagt agtgtgtgca ctgcccaaaa atgtttgttg 2581 tttatagtcg gttaaagaga aaaaagaaaa aaagatccat acacacacgt taattagttg 2641 ttcaacgtaa tacactcata ttttgttctt atttgctttc ggtcgctgtt ctcaccaaga 2701 tttattgcca acgaaacaat ttttttttat atattttcag atttttcttt ttttcctttc 2761 ctttcctttt ctaattttca ctcctggttt tctttctttc ttagaaacat tatctcgata 2821 ttaatattaa aaaaatataa tcattcaaaa tggacggtgg tatgttttag tttagcttca 2881 attctaattg attgattaat cagttgattg gtttcaatat gacaaatggg tagggtggga 2941 aaacttcatt ttcaattcag atcaaacttt tttgttgtcg acataatatt tctcgtttgg 3001 gatgttactg tcacattaat aatacacaca catcagctta taattttgaa agtaatttat 3061 cagatatgtt gtgacgatca atggaaatgg ctaacttcaa tgtatctgtt cttccccttt 3121 ttcaaagttc acgttttttg attgattgat tgatctgtcg gcagtggttt caaaaccatt 3181 cggtgagtaa tcctatcaat caatgttacg acaaaaggct caatattcaa aattgcaatg 3241 ttttatgttt tcctacgtgt acttgtgcaa ggcaattgat tcaacattgc ttttggtgtt 3301 tgacgagttt ctagtttgga cttgtgttgt tatctgggct atacagattt cccggctcac 3361 tatgaatttt ttttttcgac gctcagtgca cacaactata aacaacacaa acacaaacac 3421 agcaagaaaa aaaaaaaacg aacattgaat tgaaaccaag ccaactgaaa aattccttat 3481 ttaaatgact gtcatactaa cccattttta tagaagaagt tgctgcttta gttatcgata 3541 acggttctca tatgaaaatt tcggtgatcc ctgagcaggt ggcggaaaca ttggatgctg 3601 agaaccattt cattgttcgt gaagtgttcg atgtgcacct atccgaccaa ggctttgaac 3661 tatctaccag aagtgtgagc ccctaccgga aggattacat ctcggatgat gactctgatg 3721 aagactctgc ttgctatggc gcattcatcg accaagagct tgtcgggaag attgaactca 3781 actcaacatg gaacgatcta gcctctatcg aacacattgt tgtgtcgcac acgcaccgag 3841 gcaaaggagt cgcgcacagt ctcatcgaat ttgcgaaaaa gtgggcacta agcagacagc 3901 tccttggcat acgattagag acacgacgtc gtatagtgct tgctgttcga tattgctaga 3961 ggcaaagaaa aaggattacc tcacgcctaa aatggaattt gaaaattgag gagatgagtt 4021 gagcttttgt cttttacact ttccctattc aattgtgccg caaaaacttc aatatctaac 4081 acagtgtgaa atatttgtta tgtataccga aagccaaata ccatttaaac tggtatttca 4141 agatttcata aatcaagttt ctggttaaat taattgctgt taaacagaac aagagcttca 4201 cattgatgtt ggaataacta ctatagaatt ataaaataca caaaaccagg tcgtgtacgc 4261 aataactttt caatgtatga aaaaattttt tttttttttt tgcttcttct cttcgcttct 4321 tttttctagt tatcaatttg gcaatgaatt gatttgattg ccctttgttt agtttactat 4381 gaaaaaaaat tttttttttg tctctgtcag attttctctc attttttttt ttgcctcata 4441 atgaacaact caaaaaagaa tataaatttc tcatcaaaga cctttccaat tgaaaatttt 4501 ttctttcctt cttttccttt ttttttttct ttctttcttc ttttctaagt atacatttta 4561 tataatcaat cctcgagatg aaaaaaccag aattgactgc tacttctgtt gaaaaatttt 4621 tgattgaaaa atttgattct gtttctgatt tgatgcaatt gtctgaaggt gaagaatcaa 4681 gagctttttc ttttgatgtt ggtggtagag gttatgtttt gagagttaat tcttgtgctg 4741 atggttttta taaagataga tatgtttata gacattttgc ttctgctgct ttgccaattc 4801 cagaagtttt ggatattggt gaattttctg aatctttgac ttattgtatt tcaagaagag 4861 ctcaaggtgt tactttgcaa gatttgccag aaactgaatt gccagctgtt ttgcaaccag 4921 ttgctgaagc tatggatgct attgctgctg ctgatttgtc tcaaacttct ggttttggtc 4981 catttggtcc acaaggtatt ggtcaatata ctacttggag agattttatt tgtgctattg 5041 ctgatccaca tgtttatcat tggcaaactg ttatggatga tactgtttct gcttctgttg 5101 ctcaagcatt ggatgaattg atgttgtggg ctgaagattg tccagaagtt agacatttgg 5161 ttcatgctga ttttggttct aataatgttt tgactgataa tggtagaatt actgctgtta 5221 ttgattggtc tgaagctatg tttggtgatt ctcaatatga agttgctaat attttttttt 5281 ggagaccatg gttggcttgt atggaacaac aaactagata ttttgaaaga agacatccag 5341 aattggctgg ttctccaaga ttgagagctt atatgttgag aattggtttg gatcaattgt 5401 atcaatcttt ggttgatggt aattttgatg atgctgcttg ggctcaaggt agatgtgatg 5461 ctattgttag atcaggtgct ggtactgttg gtagaactca aattgctaga agatcagctg 5521 ctgtttggac tgatggttgt gttgaagttt tggctgattc tggtaataga agaccatcta 5581 ctagaccaag agctaaagaa taaggatccg acgaagttgg tgtatgataa cgaatttata 5641 gtttttatat tgtaaaataa tgaaatattt gttttttttt tactttgatg tatatgctgt 5701 atcccaatcc tcgatacatt gcagaattga atcatttggt ttatctgttg ttttgtgcta 5761 agtaagatat tttccatcta gaagtgatag ctttttatta tcaaattaca atccctatta 5821 catgtggaca tcaactccat ttctttgcgc attttatagt agagttagag ttagcttgtc 5881 acatctcgtt gcttcctttt gctgcaactg tttccgattt caaaatgaaa atagcttaaa 5941 gttacccgga gggcctgcac ataatcaaaa tatagtaaat tcccaaaaga acagtcgtgt 6001 gcagtttcca aagtgaaatt ttttttttgt ttctctcact tccttgtaag gttgtttccc 6061 tcttctttct ctctaaccaa tcctttgtag attgaaaatt ttttcttgca aatttttctt 6121 taactctctt tccctttttt tttcgaatca aggtaccata tcattaccaa attagacatc 6181 atgcctatgc attgttcgtg aagtgttcga tgtgcaccta tccgaccaag gctttgaact 6241 atctaccaga agtgtgagcc cctaccggaa ggattacatc tcggatgatg actctgatga 6301 agactctgct tgctatggcg cattcatcga ccaagagctt gtcgggaaga ttgaactcaa 6361 ctcaacatgg aacgatctag cctctatcga acacattgtt gtgtcgcaca cgcaccgagg 6421 caaaggagtc gcgcacagtc tcatcgaatt tgcgaaaaag tgggcactaa gcagacagct 6481 ccttggcata cgattagaga cacaaacgaa caatgtacct gcctgcaatt tgtacgcaaa 6541 atgtggcttt actctcggcg gcattgacct cttcacgtat aaaactagac ctcaagtctc 6601 gaacgaaaca gcgatgtact ggtactggtt ctcgggagca caggatgacg cctaacatat 6661 gtgaagtgtg aagggggaga ttttcacttt attagatttg tatatatgta taataaataa 6721 ataaataagt taaataaata attagataag ggtggtaatt attactattt acaatcaaag 6781 gtggtcctgc aggaagttcc tatactttct agagaatagg aacttcagat ccactagttc 6841 tagagcggtt taaacaatga ctattggtag cgtgttctat ttttcttatg cgctgaaaaa 6901 ctacgaacaa ttgtctattc caagcctgtg gctttttttg gtgattcaca atacataaat 6961 tggcaaatac ctcttctata gactttaacc tttgatgaaa tggtatggat aaaccacttt 7021 tgtggtcagg gtatatatat ttttattcca atctggcctt tataaattca aatttccaag 7081 gggactctgc taaaccttct gatataacta taactctaat aatggctgta aaaagtgtga 7141 aaaaaagatg atattcacaa aaaagataaa ggggatttca tattgggtat ctagatttaa 7201 acatcgtatt tcaacaatga aactagaatc aatacgaaat ccagggcaca actgtgttca 7261 ttcccttggg aattatactg agtaatggga agaatgtgca aaaaataccc gtgtcttata 7321 taatttccat ttgtgtaggg cttaaaagtg gacaacttca ggttacgaaa ccacgaaaat 7381 gtctaaacgt ttgatcaaat tcgtaactca tggaaaatca cagcaacaaa ttaaggatgg 7441 atatatccgg tacttacaaa taaaaacact aaagaagtat aaattttgaa ctgaaattct 7501 atagaaaatc tttttttttt gggtaaaatt atgcgattgt ggctcatagt aacgtaatca 7561 tacagttcaa caaacatcat tttgtcaaag gatttctaat cctattcttt ttctacttgg 7621 cttttgagta tatatatata tacatccttt gttcatcatc atgttcgatt gaattttttt 7681 cttggttgtt attagattgt ctacttgagt ttttcttggt gcaaattgtg gtgtttttct 7741 aacaaatttt tcaacattta aagtatttct ggaacttttc tacaaggaac gacatagatc 7801 cattaatgcg cccctctaaa acaataccca taatgtcttc ttaagtatgc atctgcttat 7861 aaatgtttct ggatttactt tagttagaat gtcgacacga caatgaacta ttgctgtaga 7921 tcacgtgcga tcactgaatt atgagtttct ttttattttt ctttttgggc tttttttttt 7981 tgcaattttt tttttccacc tttgccaatt gcgtagtcac atataatgta tacaattttt 8041 gcaactctga cactataatt gatccaataa taaagtatat aatagatcat caagtatacg 8101 taatctcccc aaaaaaaaaa ttgcataaaa taaaatctat aaaccttttt tagattttta 8161 gttgttcctt ttttctttct attcttcaac ttccaagtgt tgtcactttc ttcctgttaa 8221 ctatatttaa aagtaaatac cctccccaga aaacatg //