LOCUS pC2HP-Sti1 11585 bp ds-DNA circular SYN 07-AUG-2019 SOURCE synthetic DNA construct ORGANISM synthetic DNA construct REFERENCE 1 (bases 1 to 11585) AUTHORS . TITLE Direct Submission JOURNAL Exported Aug 7, 2019 from SnapGene Viewer 4.2.3 to Vector NTI(R) format http://www.snapgene.com COMMENT VNTDATE|885340800| COMMENT VNTDBDATE|885340800| COMMENT VNTNAME|pC2HP-Sti1| FEATURES Location/Qualifiers misc_feature 1363..2757 /label=cdARG4 misc_feature 3607..4472 /label=Integration sequence misc_feature 5272..6066 /label=KanR promoter 6314..7758 /label=Met3p misc_feature 6314..6319 /label=AatII primer 7688..7706 /label=6372 /note="sequence: CTTCAACTTCCAAGTGTTG" misc_feature 7765..7767 /label=Start codon misc_feature 7777..7797 /label=SV40 NLS misc_feature 7807..7923 /label=VP16 misc_feature 8035..8037 /label=EcoR V 1/2 site misc_feature 8038..8064 /label=Attb1 misc_feature 8065..9834 /label=Sti1 misc_feature complement(9835..9860) /label=Attb2 misc_feature 9861..9863 /label=EcoR V 1/2 site terminator 9903..10416 /label=Act terminator misc_feature 10784..11457 /label=pUCori ORIGIN 1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat 241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca 301 ctagtaacgg ccgccagtgt gctggaattc gcccttaaga acatttctgt accgcaaatg 361 tatcgacttt ttcgatggtg tgttattgtt gttttgttgc gaaatataat agcttctgct 421 ttatatcctt tgtgttgatc cttcccctcc tttcatactt gtaaaattac aattggtatt 481 ttcaaccaat attaccacac gtcatgcact aactcaaatg ggcaaaaaaa agagagatag 541 gaattacgtc attttattat gtttttacgt ggactagaag gttaagataa tttctggtta 601 ttttgttgat aattgagttt tgttcgacct ccaccaatct ttaaactctc gttgagtatt 661 cattttaatg attttttgaa atcagaaggg gtgtttctaa cccaggattg ttttattgag 721 aggttgaaac atcaatgtgt tagtttattg atttgggttg gtttaataga aaaatatcag 781 taggtgttca ataattgtag tttattttct tggctaaaat tgttgcgaaa aattgacaaa 841 ataataacaa aaagtatttt agatgctaaa acataatagt aggaatatct acctgaacca 901 tttacattca caactggcaa gaaccttcct aataatttgc aagagtaatc acaattaaac 961 caaaatcaaa gtttaccgtg tatatagttg gacagaaagc tgtcacattg gtttaataca 1021 tagttgaata tgtatgcatg aaacaacaac aatacaatta cagctcatga tccttcaatt 1081 aatttcaaca ttcaaacaac ataacacctt gtgtaaatga ggttatgagt acatgtgaac 1141 aacaaatctt atgactaaac tcgggtcgtg tacttttgac gattgagtca tttttaccaa 1201 ttaggcaatt aatttttgct aaattttcat taaccgcgct ggccatccca acaaacaata 1261 taaaagctga caacgaaaaa aaaaaaaaaa aaactccaat tctgaaaaat ttttcttctt 1321 cttcatacta aacaaaacaa ctataaagat aatcaattaa taatgtcaca acaacctagt 1381 gaaaataaat tatggggtgg tcgattcact ggcgccactg atccattaat ggatttatac 1441 aatgcttcat taccttatga taaagttatg tatgatgcag atttaactgg tactaaagta 1501 tatactcaag gattgaacaa attaggatta attaccactg aagaattgaa tctgattcat 1561 caaggtttag aacaaattag acaagaatgg catgataata aatttattat taaagctggt 1621 gatgaagata ttcatactgc taatgaacgt agattaggag aaatcattgg gaaaaacatt 1681 tctggtaaag ttcatactgg tagatccaga aatgatcaag ttgctactga tatgagaatt 1741 tttgttcgtg aatcattact taatttaagt aagattttac atcaattcat tacagcaatt 1801 cttgaacgag ctcataaaga aattgatgta ttaatgcctg gttatactca tttacaaaaa 1861 gctcaaccta ttagatgggc tcattggtta agttcatatg caacttattt cactgaagat 1921 tataaacgtt tacaagaaat cattactcgt gttaatcaat ctccattagg atctggtgct 1981 ttagctggtc atccctacgg tattgatcgt gaatttttag ctaaaggatt aggatttgat 2041 ggtgttattg ggaattcttt aactgcagtt tctgatcgtg attttgttgt tgaaagttta 2101 ttttggagta cattatttat gaatcatatt tctcgatttt cagaagattt aattatttat 2161 tctagtggtg aatttggatt tataaagtta gctgatgctt actctactgg ttcatcatta 2221 atgccacaaa agaaaaatcc tgattcatta gaattattac gtgggaaaag tggcagagtg 2281 tttggtcagt tatctgggtt tttaatgtca attaaatcca ttccatcaac ctataataaa 2341 gatatgcaag aagataaaga accattattt gatgcattga ctaccgtaga acattcaatt 2401 ttaattgcta ctggtgtgat ttctacttta ctgattgata aacaaaatat ggaaaaagct 2461 ttaactatgg atatgttagc tactgattta gcagattatt tagttagaaa aggtgttcca 2521 tttagagaaa ctcatcatat ttctggtgaa tgtgttagaa aagctgaaga agaaaaattg 2581 tcgggaattg accaattatc atttgaacaa tttcaacaaa ttgattcacg ttttgaaaaa 2641 gatgttatgg aaacttttga ttttgaagct agtgtggaaa gaagagatgc tattggtggt 2701 actgctaaga gcgctgtttt aaaacagtta gaaaatttga aatcaatttt aaattaattt 2761 ataaataatc atatagtatt caatgttttg ttttgttttt tttttttttt tatggaaatg 2821 tacaaacagt atataatcaa ttaatagaaa gataccctgt attccattgt atcgcctttg 2881 ggatgtctat aataccaact ttcaagaatg agaaattaaa caacaatttc tttcaccaat 2941 caactcgtct atattcaata aactcattct ggtcttttca aatatttaat gttatcctga 3001 tcaaacttac aaatcaaaaa tgtatcaaaa tcattcatta gttcttcttc ttttgttatc 3061 tttgtcacta tatttctctc catccagacc aaaaatactt ccattgtgaa gatcgtctga 3121 atcataatca gcaactacct cttccatttc agagaattca gatttttcaa aataggtatc 3181 acgggccaag tacacacaag acaaacgata cgagtgctgg gatatttctt tcaatgtatc 3241 actaaattcg ttgagattta aagagagccc tttgactgct ttcattactt tcctcacatc 3301 atgagcatcc acattcaaat ttcctgtagc cctttattct tagttttctt ttcttattct 3361 tttcttcgta cattgaaatc acaaatggtg atatcaaacg aaggtcacac tgacttatgt 3421 ctaatacctc caatgatgat actggtaact taaccaggtc tactcttttc aaacgatttc 3481 tcattagtgt caatttacgt aggttcttca acctttcaaa cgatgcaaat agcttttgta 3541 agggcgaatt ctgcagatat ccatcacact ggcggccggc cgctcgagca tgcatctaga 3601 gggcccttga agaattggaa gaagttagta gttaaaaaaa caatgggaaa gatttaatct 3661 ttttttcttt tttcaaaaat ttttaaccgc tgatgatata ttaatctctc tccaccactc 3721 tctttaaaca ccacaattat taataataag atatctctcc tactagttat ttgttagtaa 3781 gtatattgtt gattgattgg ttgggttaaa aatttttttt ttcataaaga aaataatgaa 3841 agtgagaaaa atttaaaccc cacattacat actcaacaca acactacaca cacgcattta 3901 acaatcatca tcagttgaaa tatattttac aaacaattac gattgtttaa tgaaaactat 3961 ttctgttgtt attattattt aacctttttt ttcttgttgg gttgaccctt tttctattaa 4021 ctatctaact accacataac atttgaagtt gaactattaa accttaattt gcggccgcaa 4081 atctaatcta ctttcttctt cttaccctat ttattctgaa atgggggggg ggggggggtg 4141 gtgggaactt taggcgtgtt aaatttgtca catctgttct cacttgcttc tattcgtata 4201 attttaattg tcttgtgaga tcaatcttgt tcttgttcga gttttttctt gctttgatta 4261 actactatta ttattagtat gcaatgctgt ttattaccat ttcataactt atattataac 4321 tcattgattt caatacctgc tcaacaatga gtcgttatta ttactgtgct ctgtattctg 4381 tactgctgca cgtacgttat gtccattgaa gacaagcaaa gaaagaccaa cattcaattg 4441 tatctgctta ccagagttgt ttacctctgt taccaatatt tggaaattaa ttttttggtg 4501 gtggacgttt cttcctaaaa cacctagatg tgctagtata attgcacaac cagcacgcac 4561 actgcgtctg gccggtcctg gactacattt tgtctctaac attcgtagct gcacgtacgg 4621 cagtttaagg tttacaccta taaaagagag agccgttatc gtctgtttgt ggatgtacag 4681 agtgatatta ttgacacgcc ggggcgacgg atggtgatcc ccctggccag tgcacgtctg 4741 ctgtcagata aagtctcccg tgaactttac ccggtggtgc atatcgggga tgaaagctgg 4801 cgcatgatga ccaccgatat ggccagtgtg ccggtctccg ttatcgggga agaagtggct 4861 gatctcagcc accgcgaaaa tgacatcaaa aacgccatta acctgatgtt ctggggaata 4921 taaatgtcag gcatgagatt atcaaaaagg atcttcacct agatcctttt cacgtagaaa 4981 gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc tatctggaca 5041 agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac atggcgatag 5101 ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg ggcgccctct 5161 ggtaaggttg ggaagccctg caaagtaaac tggatggctt tcttgccgcc aaggatctga 5221 tggcgcaggg gatcaagctc tgatcaagag acaggatgag gatcgtttcg catgattgaa 5281 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac 5341 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg 5401 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaagacgag 5461 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt 5521 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg 5581 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg 5641 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga 5701 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag 5761 gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga cggcgaggat 5821 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt 5881 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg 5941 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt 6001 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc 6061 ttctgaatta ttaacgctta caatttcctg atgcggtatt ttctccttac gcatctgtgc 6121 ggtatttcac accgcatcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg 6181 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat 6241 gcttcaataa tagcacgtga ggagggccac catggccaag ttgaccagtg ccgttccggt 6301 gctcaccgcg cgcgacgtcg tctattccaa gcctgtggct ttttttggtg attcacaata 6361 cataaattgg caaatacctc ttctatagac tttaaccttt gatgaaatgg tatgtctatt 6421 ccaagcctgt ggcttttttt ggtgattcac aatacataaa ttggcaaata cctcttctat 6481 agactttaac ctttgatgaa atggtatgga taaaccactt ttgtggtcag ggtatatata 6541 tttttattcc aatctggcct ttataaattc aaatttccaa ggggactctg ctaaaccttc 6601 tgatataact ataactctaa taatggctgt aaaaagtgtg aaaaaaagat gatattcaca 6661 aaaaagataa aggggatttc atattgggta tctagattta aacatcgtat ttcaacaatg 6721 aaactagaat caatacgaaa tccagggcac aactgtgttc attcccttgg gaattatact 6781 gagtaatggg aagaatgtgc aaaaaatacc cgtgtcttat ataatttcca tttgtgtagg 6841 gcttaaaagt ggacaacttc aggttacgaa accacgaaaa tgtctaaacg tttgatcaaa 6901 ttcgtaactc atggaaaatc acagcaacaa attaaggatg gatatatccg gtacttacaa 6961 ataaaaacac taaagaagta taaattttga actgaaattc tatagaaaat cttttttttt 7021 gggtaaaatt atgcgattgt ggctcatagt aacgtaatca tacagttcaa caaacatcat 7081 tttgtcaaag gatttctaat cctattcttt ttctacttgg cttttgagta tatatatata 7141 tatatataca tcctttgttc atcatcatgt tcgattgaat ttttttcttg gttgttatta 7201 gattgtctac ttgagttttt cttggtgcaa attgtggtgt ttttctaaca aatttttcaa 7261 catttaaagt atttctggac ttttctacaa ggaacgacat agatccatta atgcgcccct 7321 ctaaaacaat acccataatg tcttcttaag tatgcatctg cttataaatg tttctggatt 7381 tactttagtt agaatgtcga cacgacaatg aactattgct gtagatcacg tgcgatcact 7441 gaattatgag tttcttttta tttttctttt tgggcttttt tttttgcaat tttttttttc 7501 cacctttgcc aattgcgtag tcacatataa tgtatacaat ttttgcaact ctgacactat 7561 aattgatcca ataataaagt atataataga tcatcaagta tacgtaatct ccccaaaaaa 7621 aaaattgcat aaaataaaat ctataaacct tttttagatt tttagttgtt ccttttttct 7681 ttctattctt caacttccaa gtgttgtcac tttcttcctg ttaactatat ttaaaagtaa 7741 ataccctccc cagaaaactc cggaatgggt gctcctccaa aaaagaagag aaaggtagct 7801 ccgcggtctc cagggcccgg tttcacccca cacgattctg ctccatacgg tgctttggat 7861 atggctgatt tcgaattcga acaaatgttc accgatgctt tgggtattga tgaatacggt 7921 ggtgctagcg gtgctggtgg tgttggtgac tacaaagacc atgacggtga ttataaagat 7981 catgacatcg attacaagga tgacgatgac aagggagcag gaggcgtcgg aaggatcaca 8041 agtttgtaca aaaaagcagg cttgatgaca acagctgacg aatacaaagc agaaggtaac 8101 aaatattttg ctgctaaaga ttttgaaaag gcgattgaag cattcactaa agcaattgaa 8161 gcatcacctg aaccaaacca tgttctttat tcaaatcgtt ctggatctta tgcctcttta 8221 aaagatttta acaacgcatt aaaagatgct caagaatgtg tcaagatcaa tcctagttgg 8281 gccaaagggt ataatagaat tgctggggct gaatttggtt taggtaattt tgatcaagct 8341 aaatccaatt atgaaaaatg tttggagttg gatccaaata atgccatggc taaagaaggt 8401 ttaaaatcag ttgaatctgc tttagtatct ggtggtggtg atgatgacaa ggatttagga 8461 tttggtaaaa ttttgaatga tcctaatctt tatactaaat tgaaaaataa tccaaaaaca 8521 agtgaattta tgaatgatcc tcaatttgtt gctaaacttg aacgtcttaa aactaatcca 8581 caattgggta atcctgatat gtttagtgat ccaagattat tgacggcttt tgctgcttta 8641 atgggtattg acatggattt accaaatatg ggattcactg ctccaaacga atcacaatcc 8701 aatgcatcag aaccaaaact ggaaccaaaa tcagtaccag aatctaaacc agaaccaaaa 8761 gcagaacaaa aggaagaaga atcaacctca gccaaagatg aagacactcc aatgactgat 8821 gcccaagacg acactaatga taatgatgcc aaaacccaag ctgacaatgc taaagctgaa 8881 ggtaatgcct tatacaagaa acgtcaattt gatgaagcaa ttgccgccta taataaggct 8941 tgggaattac ataaggatat cacttattta aacaatcgtg ctgctgccga atatgaaaaa 9001 ggtgattatg atgctgctat tgctacatgt gaaaaggcca ttgatgaagg tagagacatg 9061 agagctgatt ataaattgat tgctaaatca tttgctagat taggtaatat ttatttgaaa 9121 aaagatgaat tacccgaagc agtgaaaaat tttgaaaaat ctttaactga acatcgtacc 9181 cctgatgttt taaataaatt aagatcaact caacgtgaaa ttaaaactag agaattaaat 9241 gcttatatag atccagaaaa ggctgaagaa gcaagattac aaggtaaaga atatttcacc 9301 aaaggagatt ggccaaatgc cgttaaggct tatactgaaa tgattaaaag agcaccagaa 9361 gatgctagag gatattctaa tcgtgctgct gcattggcaa aattgttatc ctttcctgat 9421 gctatacaag attgtaataa agccattgaa aaagatccaa atttcattag agcttatatt 9481 agaaaagcta atgctcaatt ggcaatgaaa gaatatagtc atgtcatgga tactttaacc 9541 gaggcaagaa ctaaagatgt tgaattgggt ggtaaatcaa ttcatgaaat tgatgaatta 9601 atgaataaag ctacttatca aagatttcaa gccattgaag gtgaaactcc tgaacaaact 9661 atggaaagag tttctaaaga tccagaaatt gttcaaattt tacaagatcc agtaatgcaa 9721 ggaattttag ctcaagctag agaaaatcct gctgctttac aagatcatat gaaaaatcct 9781 gaagtttata aaaaaattaa tatgttgatt gctgctggtg ttattcgtac cagagaccca 9841 gctttcttgt acaaagtggt gatcgcgtag tacttaagat cctgaagacg ggatcgttcg 9901 aagagtgaaa ttctggaaat ctggaaatct ggttttgtat tcttgttatt cttctttttg 9961 ttattacata tataacttgt tactttttta aaaaaatctt tgtttatttt ataaatatat 10021 aaaactaaat ttaagaaaaa gagaaaaatg ttttatttga gagattgaaa ttttacttga 10081 atttagctta gcttttataa agtattatta tgtaaaaaaa caaaacaaat atacattaaa 10141 aagttaagac tataaaatag ccacccaagg catttctata tcttgttgtt gttgttttca 10201 tcttctgtat cagaggaact tattttatta ttttcgtcac gggtattttc tcttgtttga 10261 tgattcatcc cattcattcc atcataaaat tctagaagga ccacctttga ttgtaaatag 10321 taataattac cacccttatt taattattta tttaacttat ttatttattt attatacata 10381 tatacaaatc taataaagtg aaaatctccc ccttcacacc ggtgtggtcc gggacgacgt 10441 gaccctgttc atcagcgcgg tccaggacca ggtggtgccg gacaacaccc tggcctgggt 10501 gtgggtgcgc ggcctggacg agctgtacgc cgagtggtcg gaggtcgtgt ccacgaactt 10561 ccgggacgcc tccgggccgg ccatgaccga gatcggcgag cagccgtggg ggcgggagtt 10621 cgccctgcgc gacccggccg gcaactgcgt gcacttcgtg gccgaggagc aggactgaca 10681 cgtgctaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct 10741 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 10801 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 10861 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 10921 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta 10981 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 11041 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 11101 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 11161 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 11221 cacgcctccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 11281 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 11341 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 11401 gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 11461 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 11521 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc 11581 ggaag //