LOCUS       pC2HB-Crk1             11457 bp    DNA     circular UNA 15-MAY-2019
DEFINITION  LMBP 11401.
SOURCE      
  ORGANISM  
COMMENT     This file is created by Vector NTI
            http://www.invitrogen.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTDATE|-12815212|
COMMENT     VNTDBDATE|-12815212|
COMMENT     LSOWNER|
COMMENT     VNTNAME|pC2HB-Crk1|
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COMMENT     VNTOAUTHORNAME|UNKNOWN|
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FEATURES             Location/Qualifiers
     gene            321..2503
                     /vntifkey="60"
     misc_feature    2741..3854
                     /feature_type="integratie sequentie"
                     /vntifkey="21"
                     /label=IntegSeq
     KanR            4499..5402
                     /vntifkey="21"
                     /label=Misc_Feature_1
                     /note="Unknown feature type:KanR"
     KanR            4528..4530
                     /vntifkey="21"
                     /label=KanR\start\codon
                     /note="Unknown feature type:KanR"
     promoter        5563..7002
                     /vntifkey="30"
                     /label=MET3p
     primer          6932..6950
                     /vntifkey="27"
                     /label=6372
     misc_feature    7009..7011
                     /feature_type="Start codon"
                     /vntifkey="21"
                     /label=Start\codon
     misc_signal     7021..7041
                     /vntifkey="87"
                     /label=SV40
     CDS             7045..7662
                     /cds_type=ORF
                     /vntifkey="4"
                     /label=LexA
     linker          7669..7686
                     /vntifkey="21"
                     /label=glycine\linker
                     /note="Unknown feature type:linker"
     misc_feature    7687..7713
                     /feature_type="Ha tag"
                     /vntifkey="21"
                     /label=HA\tag
     misc_feature    7714..7731
                     /feature_type=Misc.
                     /vntifkey="21"
                     /label=Glycine\linker
     CDS             7744..9984
                     /cds_type=ORF
                     /vntifkey="4"
                     /label=Crk1
     terminator      10013..10526
                     /vntifkey="43"
                     /label=CaACT1term
     rep_origin      10656..11329
                     /vntifkey="33"
                     /label=pUCori
BASE COUNT     3491 a      2227 c      2441 g      3298 t 
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc 
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc 
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa 
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat 
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca 
      301 ctagtaacgg ccgccagtgt gctggaattc gcccttggat ccaatcatca ctggtggggg 
      361 tcaagaaaga ggtttgagat caggtacttt ggcaccacca ttagtagctg ggtttggtga 
      421 agctgcaaga ttaatgaaac aagaatctgc atttgacaag aaacatattg agaggttatc 
      481 tactaaattg aagaacggtt tgttatccat tccatctact cagtttaacg gttgtaataa 
      541 cccaacatat caatacccag gttgtgtgaa tgtttccttt gcatacattg aaggggaatc 
      601 tttgttaatg gcattgaaag atattgcatt gagttctggt tctgcatgta cctctgcatc 
      661 tttggaacca tcgtatgttt tacatgcttt gggtgccgat gatgccttgg ctcattcttc 
      721 cattagattc ggtattggta gattcaccac tgaagcagaa gttgactatg ttattcaagc 
      781 aattaatgaa agagttgatt tcttgagaaa gatgtctcca ttatgggaaa tggttcaaga 
      841 aggtattgac ttgaactcta tcgaatggag tggacattaa ggttttttgt ttttttgtaa 
      901 tttgtgttgt tatgtgtaat tatcgtgtat agagtaattt cggaaattgt actttcgttc 
      961 atgtatctga accagatgaa taatacgttc gtattgttga agaatgacta actcctgtaa 
     1021 cggcgtgcgt gggttccgca gccgggttca attttttttt tcttgttcac tataaaaata 
     1081 tataataaac gaaaaaaatc cgagggactc aggctatgaa atttttcact caagaatttt 
     1141 ttttccttca tccttaattc ctttattcac tacccaaacc cacaatgtca gttaaaacta 
     1201 aaactattac aattttacca ggtgatcacg tcggtaccga aattgtcaat gaagctatca 
     1261 aagttttaga agcaattgaa gctgccactc cttaccaaaa aatccatttc gatttcaaac 
     1321 atcatttaat cggtggtgct gccattgatg ccactggtgt cccattgcca gatgacgcac 
     1381 ttgaaagtgc caaaaaatct gatgccgtct tattgggtgc tgttggtgga ccaaaatggg 
     1441 ggactggcgc cgttcgtcca gaacaaggtt tattaaagat tcgtaaagaa ttgaaccttt 
     1501 atgctaacat cagaccatgt aactttgcca gtgactcctt attggaatta tccccattga 
     1561 gaccagaagt cgttaaaggt acaaacttga tcattgttcg tgaattagtt ggtggtattt 
     1621 atttcggtga tcgtgaagaa caagaagaaa gtgaagataa acaaaccgca tgggatactg 
     1681 aaaaatacac cgtcgatgaa gtcaccagaa tcacccgtat ggctgccttc atggctttac 
     1741 aacataaccc accattacca atctggtcat tggataaggc taatgtttta gcttcttcta 
     1801 gattatggag aagaactgtc gataaagtga tttctgaaga gttcccaact ttgtctgttc 
     1861 aacatcaatt gattgactcc gccgccatga ttttaattca aaacccaacc aaattgaatg 
     1921 gtataatcat cacttctaac atgtttggtg atatcatttc cgatgaagct tcagttattc 
     1981 caggttcctt gggtttatta ccatctgcat ctttggcttc attgccagat accaacaccg 
     2041 cttttggtct ttatgaacca tgtcacggtt ctgctcctga tttaccagcc aataaagtta 
     2101 acccaattgc tactatttta tctgctgctt ctatgttgag attatctttg gattgtgtga 
     2161 aagaagctga agctttagaa gaagccgtga aacaagtctt ggataaaggt atcagaactg 
     2221 cagatttaag aggtaccagt tcagctactg aagttggtga cgcgattgtc gaagctgtta 
     2281 ctaaaatctt aaaagaaaaa gcttaagtaa agtatacaca aaatttaacg aatattatta 
     2341 atacaaaatg aacaccacct aaatactttt ttttaaaaat gtaccgactc ttctaaaaat 
     2401 tgcgataatt caatatcgga aatttctctc accggtttac ttggatcttc ggaaataaat 
     2461 ctatttgaag taatggtagc ctcacttgat tctgattggc tctttctaga catgggtagg 
     2521 taagggcgaa ttctgcagat atccatcaca ctggcggccg gccgctcgag catgcatcta 
     2581 gagggcccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac 
     2641 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt 
     2701 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg accagatcaa aatgctagaa 
     2761 caaattacgt ctgcatcaac agtcatcttc cttatttgca taaatgctaa aatggcaaac 
     2821 ctgtaaaccg tttgaaaagc aaattgagat agttagctcg gttttttttt cacgtggcca 
     2881 taatccaaca aaaaaaagga atttaagggg ggagagaagg aggcttgaat ttgtaccaca 
     2941 gagcgagcaa gaaattaata atatacttgg cttagctgaa atgagaatga aaaagcaaat 
     3001 ttcaagtgaa acaccactac tagccaagta aattggccaa tttgctctcg tgctttaatt 
     3061 tcctaacaag cttttgagac cgaaaaaaat caaccaatga tgatggaaaa atacttccgg 
     3121 gagtcaattt atccataatt tcacgtcgtt ttttttttgc acatggcaaa tatattggaa 
     3181 tcctgagatt ttcttggagt cgtgtacaat aacaccaggc gggaacatgc cgttatgatc 
     3241 cagcatagcg gccgctatgt gttcaaatat tgaaaccgtg aaaccagttt gatacagctg 
     3301 atattgtgtg ctaaaaaaag atgagcaccg aaagagtctt tagtttattg ccaagtaaag 
     3361 ggtcaaagaa tacctattct ttatgaacac agacttgctc taatggtgaa ttagtgtgga 
     3421 gtaatagaga aggaggcgaa acaaaaataa ttggttaaac actcgaatgt cacacaatcc 
     3481 taaataggtt aaattcagtt accgccgtga tgcgggaaca aagccgagcg gtaatatttt 
     3541 tggattctat gtttttttgg tgtatattgc acgacttggt gccaacaact ggtgttccaa 
     3601 cgcacaaaac taggttcaac tggatactgt tttctagaaa ttatttgcgg caattcatga 
     3661 ttacggtcct aaatttgaaa taaaagtttc caattatgtg tcttgacaat ttccattggg 
     3721 ttcaaaaaga tctagaatgt atgtaattac gcaacaattt gcatgcaaag aaataaaaac 
     3781 aaacctaaaa tccaactgct gttgttctgg tgatgatgta gtaaaataag agcacggcca 
     3841 agaacaaggt gatttacgta cggcagttta aggtttacac ctataaaaga gagagccgtt 
     3901 atcgtctgtt tgtggatgta cagagtgata ttattgacac gccggggcga cggatggtga 
     3961 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg 
     4021 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct 
     4081 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca 
     4141 ttaacctgat gttctgggga atataaatgt caggcatgag attatcaaaa aggatcttca 
     4201 cctagatcct tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg 
     4261 tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt 
     4321 gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg 
     4381 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg 
     4441 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag ctctgatcaa gagacaggat 
     4501 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg 
     4561 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg 
     4621 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg 
     4681 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc 
     4741 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg 
     4801 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca 
     4861 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc 
     4921 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg 
     4981 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg 
     5041 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata 
     5101 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg 
     5161 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat 
     5221 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct 
     5281 tctatcgcct tcttgacgag ttcttctgaa ttattaacgc ttacaatttc ctgatgcggt 
     5341 attttctcct tacgcatctg tgcggtattt cacaccgcat caggtggcac ttttcgggga 
     5401 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc 
     5461 atgagacaat aaccctgata aatgcttcaa taatagcacg tgaggagggc caccatggcc 
     5521 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcttccaatt agaggttgtg 
     5581 gatgcaaaat tgaaaaaaaa atgactattg gtagcgtgtt ctatttttct tatgcgctga 
     5641 aaaactacga acaattgtct attccaagcc tgtggctttt tttggtgatt cacaatacat 
     5701 aaattggcaa atacctcttc tatagacttt aacctttgat gaaatggtat ggataaacca 
     5761 cttttgtggt cagggtatat atatttttat tccaatctgg cctttataaa ttcaaatttc 
     5821 caaggggact ctgctaaacc ttctgatata actataactc taataatggc tgtaaaaagt 
     5881 gtgaaaaaaa gatgatattc acaaaaaaga taaaggggat ttcatattgg gtatctagat 
     5941 ttaaacatcg tatttcaaca atgaaactag aatcaatacg aaatccaggg cacaactgtg 
     6001 ttcattccct tgggaattat actgagtaat gggaagaatg tgcaaaaaat acccgtgtct 
     6061 tatataattt ccatttgtgt agggcttaaa agtggacaac ttcaggttac gaaaccacga 
     6121 aaatgtctaa acgtttgatc aaattcgtaa ctcatggaaa atcacagcaa caaattaagg 
     6181 atggatatat ccggtactta caaataaaaa cactaaagaa gtataaattt tgaactgaaa 
     6241 ttctatagaa aatctttttt tttgggtaaa attatgcgat tgtggctcat agtaacgtaa 
     6301 tcatacagtt caacaaacat cattttgtca aaggatttct aatcctattc tttttctact 
     6361 tggcttttga gtatatatat atatatatat acatcctttg ttcatcatca tgttcgattg 
     6421 aatttttttc ttggttgtta ttagattgtc tacttgagtt tttcttggtg caaattgtgg 
     6481 tgtttttcta acaaattttt caacatttaa agtatttctg gaacttttct acaaggaacg 
     6541 acatagatcc attaatgcgc ccctctaaaa caatacccat aatgtcttct taagtatgca 
     6601 tctgcttata aatgtttctg gatttacttt agttagaatg tcgacacgac aatgaactat 
     6661 tgctgtagat cacgtgcgat cactgaatta tgagtttctt tttatttttc tttttgggct 
     6721 tttttttttg caattttttt tttccacctt tgccaattgc gtagtcacat ataatgtata 
     6781 caatttttgc aactctgaca ctataattga tccaataata aagtatataa tagatcatca 
     6841 agtatacgta atctccccaa aaaaaaaatt gcataaaata aaatctataa acctttttta 
     6901 gatttttagt tgttcctttt ttctttctat tcttcaactt ccaagtgttg tcactttctt 
     6961 cctgttaact atatttaaaa gtaaataccc tccccagaaa actccggaat ggctgctcct 
     7021 ccaaaaaaga agagaaaggt agctagagaa ttaacaaaac gacaaagcga aatatataac 
     7081 tatattaaac aagttgttca aatgaaaggt tatccgccta gtgttcgcga aattggtgag 
     7141 gcagttggct tagcatccag ttcaactgtt catggtcacc tttcacgtct tgaagaaaaa 
     7201 ggctatataa gaagagatcc aacgaaacca cgtgctatag aaattgtaag tgatcaaaca 
     7261 aatgataata ttaatatgga agaaacgatt catgtgccag ttattggtaa agtcacagca 
     7321 ggtgttccta ttaccgcagt agaaaatatt gaagaatatt ttccattacc tgaacactta 
     7381 acatcgacac acaatagcga catattcata ttaaacgtcg taggcgacag tatgattgag 
     7441 gctggtatat tagacggaga caaagtaatt gttcgcagtc aaaccatagc agaaaatgga 
     7501 gacattattg ttgctatgac tgaggaagat gaagcaactg tcaaacgctt ctataaagaa 
     7561 aaaaatcgtt atcgattaca acctgaaaat agtacaatgg agccaattta cctcgacaat 
     7621 gttgctgtaa tcgggaaagt aattggtttg taccgcgaaa tgagatctgg tgctggtggt 
     7681 gttggttatc cgtatgatgt gcctgactac gcaggtgctg gtggtgttgg tgctagcagg 
     7741 cctatgtctg ttattgctgg ccatcatgtg ccgaggtcaa atgatcaaag gcaatacgat 
     7801 acaccgagcg taccaataaa tatcgctccg gattcagaag gtcatattca tgaaatgtct 
     7861 cgattgaaag attatgaagt gatagagaaa ctaggtcaag gaacatttgg agttgttcaa 
     7921 aaagcaaaat ccaaaaaaga tggcctgtta gttgcaatta aacaattgat taaccattct 
     7981 gcaaaggaag gattcccaat cactgccatg cgagaaatta ccattttgaa acaattaaat 
     8041 catcaaaata ttttaactat acaggatatg atatttgaag aacccaaaat gagcaatcga 
     8101 acagatatta ttactatgag gggttctttt tatactgtaa ccccatacat gtcgagtgat 
     8161 cttgttggat tactagagaa cccgaaaatt aaacttgaac ttggacaaat aaaatgtatc 
     8221 atgcaacaat tattgaaagg tattcaatat gtacacaacc aaaagttttt gcatcgagac 
     8281 attaaagcag ctaacatttt aatcggtcac gatggtgttt tgaaaattgc agatttcggt 
     8341 ttagcaagaa tatatcatgg taacgtaccg agattgggga tgggtcctgg tggaggagaa 
     8401 aaagcatata ctgggttggt tgtaactaga tggtatcgac caccagaaat attactagga 
     8461 gaaagaaaat atactactgc agttgatctt tgggggattg gatgtgtatt tgccgaatta 
     8521 ttcactggga aaccaatttt ggtagggaaa tcagattctc atcaagcaca aattgtattt 
     8581 gagttggttg gatcaccttt gacatggact gatgctgcaa aactacccaa taaaaatgaa 
     8641 tatagttgtg gcttggcttg taaacgaagt cttgaagcaa agtttgctag tatcatgcct 
     8701 actgaagcta ttgacttgtt aagtggattg ttaacattag atcctttcaa gagacttaat 
     8761 gccttggatg ctttgaatca caaatttttc agtactgatc cattaccatt attaccaacc 
     8821 caaatgccta aatttgaaga aagtcacgaa attgataaag aacgatttaa aaaattgaaa 
     8881 gataaagaac aagctgttag tgagttgaag cctccaactg aaatacgtta cgacaatcat 
     8941 tcggaatcga ggtataatgc agaccattct acttttggtg gtggtggtgt tggcggtaaa 
     9001 gaaacaagtt ttagttccgg gaaatcagac tatatcgacc attatgaacc aagagcacgt 
     9061 agggaccatt atgaaccgag aatacgaaat gataataaag attccaacga tgttagagga 
     9121 gagtttgaac tggctacaag acaagaacaa agacggaggg atattcaaaa taggcttgat 
     9181 gcaggtggaa tggatactta cattccaaaa actaccacag gaaaacttcg ggaacacagt 
     9241 ggtacagagt ctttgctgca aaagtatgat aactatcaac caataaacgt ctccagagga 
     9301 tcaaaatctc catctccgtc aaaactcctg tctatttcac aatcaaaggc agatctcatt 
     9361 agtaaaccat cggctccaaa ggttgcatct agagaaagtt cgctcgagag gaagcaagtt 
     9421 tcaaatggta tccgtactac taccgatgtg gaaccaccaa gagcaagggc tcgtcgacca 
     9481 actgacatgt ttggaagacc gttgacttca aatagtactc aggcacaacc aacaagaaac 
     9541 aaatcggtgg agcgaccaaa agatcttgag aaacctacaa atggtgtgac tgaggatagg 
     9601 aataagaaac cagttttgga agagaagaaa gaagttgtca aaccaaattt ggcaattcca 
     9661 aaaatcaaaa agagcagttc tctagtgctg ttgctgtcta gatcgtccac aacaccagtt 
     9721 atttctaatc cgtcgaaggt cacaaaaaga gcagcaagta gtgtgacacc accagtgcta 
     9781 ccaaagaaac caaaaatatc caaaactagt tcagaaagtg aagtaagtga tttggaagaa 
     9841 gacagtgatt ttacaggtga gaatgcaact gtatttgaaa ggtttatggc acttgaacaa 
     9901 ttacaaaaat cgccagttta taaaagaatt ataaatgaga aaatgaggtt tgaaaagtta 
     9961 tcgggaggac acaaatctat gtaaacgcgt agtacttaac cgcgccgtcg acgagtgaaa 
    10021 ttctggaaat ctggaaatct ggttttgtat tcttgttatt cttctttttg ttattacata 
    10081 tataacttgt tactttttta aaaaaatctt tgtttatttt ataaatatat aaaactaaat 
    10141 ttaagaaaaa gagaaaaatg ttttatttga gagattgaaa ttttacttga atttagctta 
    10201 gcttttataa agtattatta tgtaaaaaaa caaaacaaat atacattaaa aagttaagac 
    10261 tataaaatag ccacccaagg catttctata tcttgttgtt gttgttttca tcttctgtat 
    10321 cagaggaact tattttatta ttttcgtcac gggtattttc tcttgtttga tgattcatcc 
    10381 cattcattcc atcataaaat tctagaagga ccacctttga ttgtaaatag taataattac 
    10441 cacccttatt taattattta tttaacttat ttatttattt attatacata tatacaaatc 
    10501 taataaagtg aaaatctccc ccttcacacg tggccgagga gcaggactga cacgtgctaa 
    10561 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca 
    10621 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag 
    10681 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac 
    10741 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa 
    10801 ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc 
    10861 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag 
    10921 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac 
    10981 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc 
    11041 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc 
    11101 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca 
    11161 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc 
    11221 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg 
    11281 ccagcaacgc ggccttttta cggttcctgg gcttttgctg gccttttgct cacatgttct 
    11341 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata 
    11401 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaag 
//
