LOCUS       pC2HB-Sti1     10989 bp    DNA     circular UNA 22-feb-2014
DEFINITION  LMBP 11424.
FEATURES             Location/Qualifiers
     gene            321..2503
                     /label=CmLEU2
     misc_feature    2741..3854
                     /feature_type="integratie sequentie"
                     /label=IntegSeq
     Site            3248..3255
                     /site_type="restriction site"
                     /label=NotI
     KanR            4499..5402
     KanR            4528..4530
                     /label="KanR start codon"
     Site            5557..5562
                     /site_type="restriction site"
                     /label=AatII
     promoter        5563..7002
                     /label=MET3p
     primer          6932..6950
                     /label=6372
     Site            join(7003..7008,7003^7004)
                     /site_type="restriction site"
                     /note="Name: BspEI"
                     /note="Pattern: tccgga"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 7008^7009"
                     /note="cut_0_on_negative_strand: 7012^7013"
                     /note="inhibited_by: "
                     /note="site_type: other"
                     /note="restriction site"
                     /label=BspEI
     misc_feature    7009..7011
                     /feature_type="Start codon"
                     /label="Start codon"
     misc_signal     7021..7041
                     /label=SV40
     CDS             7045..7662
                     /cds_type=ORF
                     /label=LexA
     linker          7669..7686
                     /label="glycine linker"
     misc_feature    7687..7713
                     /feature_type="Ha tag"
                     /label="HA tag"
     misc_feature    7714..7731
                     /feature_type=Misc.
                     /label="Glycine linker"
     Site            7732..7737
                     /site_type="restriction site"
                     /label=NheI
     Site            7738..7743
                     /site_type="restriction site"
                     /label=StuI
     CDS             7744..9516
                     /cds_type=ORF
                     /label=Sti1
     Site            9517..9522
                     /site_type="restriction site"
                     /label=MluI
     Site            join(9523..9528,9525^9526)
                     /site_type="restriction site"
                     /note="Name: ScaI"
                     /note="Pattern: agtact"
                     /note="Number_of_matches: 1"
                     /note="cut_0_on_positive_strand: 7772^7773"
                     /note="cut_0_on_negative_strand: 7772^7773"
                     /note="inhibited_by: 5': N4-methylcytosine"
                     /note="star_activity: yes"
                     /note="site_type: other"
                     /note="restriction site"
                     /label=ScaI
     terminator      9545..10058
                     /label=CaACT1term
     rep_origin      10188..10861
                     /label=pUCori
ORIGIN
        1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
       61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
      121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
      181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagctat
      241 ttaggtgaca ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca
      301 ctagtaacgg ccgccagtgt gctggaattc gcccttggat ccaatcatca ctggtggggg
      361 tcaagaaaga ggtttgagat caggtacttt ggcaccacca ttagtagctg ggtttggtga
      421 agctgcaaga ttaatgaaac aagaatctgc atttgacaag aaacatattg agaggttatc
      481 tactaaattg aagaacggtt tgttatccat tccatctact cagtttaacg gttgtaataa
      541 cccaacatat caatacccag gttgtgtgaa tgtttccttt gcatacattg aaggggaatc
      601 tttgttaatg gcattgaaag atattgcatt gagttctggt tctgcatgta cctctgcatc
      661 tttggaacca tcgtatgttt tacatgcttt gggtgccgat gatgccttgg ctcattcttc
      721 cattagattc ggtattggta gattcaccac tgaagcagaa gttgactatg ttattcaagc
      781 aattaatgaa agagttgatt tcttgagaaa gatgtctcca ttatgggaaa tggttcaaga
      841 aggtattgac ttgaactcta tcgaatggag tggacattaa ggttttttgt ttttttgtaa
      901 tttgtgttgt tatgtgtaat tatcgtgtat agagtaattt cggaaattgt actttcgttc
      961 atgtatctga accagatgaa taatacgttc gtattgttga agaatgacta actcctgtaa
     1021 cggcgtgcgt gggttccgca gccgggttca attttttttt tcttgttcac tataaaaata
     1081 tataataaac gaaaaaaatc cgagggactc aggctatgaa atttttcact caagaatttt
     1141 ttttccttca tccttaattc ctttattcac tacccaaacc cacaatgtca gttaaaacta
     1201 aaactattac aattttacca ggtgatcacg tcggtaccga aattgtcaat gaagctatca
     1261 aagttttaga agcaattgaa gctgccactc cttaccaaaa aatccatttc gatttcaaac
     1321 atcatttaat cggtggtgct gccattgatg ccactggtgt cccattgcca gatgacgcac
     1381 ttgaaagtgc caaaaaatct gatgccgtct tattgggtgc tgttggtgga ccaaaatggg
     1441 ggactggcgc cgttcgtcca gaacaaggtt tattaaagat tcgtaaagaa ttgaaccttt
     1501 atgctaacat cagaccatgt aactttgcca gtgactcctt attggaatta tccccattga
     1561 gaccagaagt cgttaaaggt acaaacttga tcattgttcg tgaattagtt ggtggtattt
     1621 atttcggtga tcgtgaagaa caagaagaaa gtgaagataa acaaaccgca tgggatactg
     1681 aaaaatacac cgtcgatgaa gtcaccagaa tcacccgtat ggctgccttc atggctttac
     1741 aacataaccc accattacca atctggtcat tggataaggc taatgtttta gcttcttcta
     1801 gattatggag aagaactgtc gataaagtga tttctgaaga gttcccaact ttgtctgttc
     1861 aacatcaatt gattgactcc gccgccatga ttttaattca aaacccaacc aaattgaatg
     1921 gtataatcat cacttctaac atgtttggtg atatcatttc cgatgaagct tcagttattc
     1981 caggttcctt gggtttatta ccatctgcat ctttggcttc attgccagat accaacaccg
     2041 cttttggtct ttatgaacca tgtcacggtt ctgctcctga tttaccagcc aataaagtta
     2101 acccaattgc tactatttta tctgctgctt ctatgttgag attatctttg gattgtgtga
     2161 aagaagctga agctttagaa gaagccgtga aacaagtctt ggataaaggt atcagaactg
     2221 cagatttaag aggtaccagt tcagctactg aagttggtga cgcgattgtc gaagctgtta
     2281 ctaaaatctt aaaagaaaaa gcttaagtaa agtatacaca aaatttaacg aatattatta
     2341 atacaaaatg aacaccacct aaatactttt ttttaaaaat gtaccgactc ttctaaaaat
     2401 tgcgataatt caatatcgga aatttctctc accggtttac ttggatcttc ggaaataaat
     2461 ctatttgaag taatggtagc ctcacttgat tctgattggc tctttctaga catgggtagg
     2521 taagggcgaa ttctgcagat atccatcaca ctggcggccg gccgctcgag catgcatcta
     2581 gagggcccaa ttcgccctat agtgagtcgt attacaattc actggccgtc gttttacaac
     2641 gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     2701 tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg accagatcaa aatgctagaa
     2761 caaattacgt ctgcatcaac agtcatcttc cttatttgca taaatgctaa aatggcaaac
     2821 ctgtaaaccg tttgaaaagc aaattgagat agttagctcg gttttttttt cacgtggcca
     2881 taatccaaca aaaaaaagga atttaagggg ggagagaagg aggcttgaat ttgtaccaca
     2941 gagcgagcaa gaaattaata atatacttgg cttagctgaa atgagaatga aaaagcaaat
     3001 ttcaagtgaa acaccactac tagccaagta aattggccaa tttgctctcg tgctttaatt
     3061 tcctaacaag cttttgagac cgaaaaaaat caaccaatga tgatggaaaa atacttccgg
     3121 gagtcaattt atccataatt tcacgtcgtt ttttttttgc acatggcaaa tatattggaa
     3181 tcctgagatt ttcttggagt cgtgtacaat aacaccaggc gggaacatgc cgttatgatc
     3241 cagcatagcg gccgctatgt gttcaaatat tgaaaccgtg aaaccagttt gatacagctg
     3301 atattgtgtg ctaaaaaaag atgagcaccg aaagagtctt tagtttattg ccaagtaaag
     3361 ggtcaaagaa tacctattct ttatgaacac agacttgctc taatggtgaa ttagtgtgga
     3421 gtaatagaga aggaggcgaa acaaaaataa ttggttaaac actcgaatgt cacacaatcc
     3481 taaataggtt aaattcagtt accgccgtga tgcgggaaca aagccgagcg gtaatatttt
     3541 tggattctat gtttttttgg tgtatattgc acgacttggt gccaacaact ggtgttccaa
     3601 cgcacaaaac taggttcaac tggatactgt tttctagaaa ttatttgcgg caattcatga
     3661 ttacggtcct aaatttgaaa taaaagtttc caattatgtg tcttgacaat ttccattggg
     3721 ttcaaaaaga tctagaatgt atgtaattac gcaacaattt gcatgcaaag aaataaaaac
     3781 aaacctaaaa tccaactgct gttgttctgg tgatgatgta gtaaaataag agcacggcca
     3841 agaacaaggt gatttacgta cggcagttta aggtttacac ctataaaaga gagagccgtt
     3901 atcgtctgtt tgtggatgta cagagtgata ttattgacac gccggggcga cggatggtga
     3961 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg
     4021 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct
     4081 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
     4141 ttaacctgat gttctgggga atataaatgt caggcatgag attatcaaaa aggatcttca
     4201 cctagatcct tttcacgtag aaagccagtc cgcagaaacg gtgctgaccc cggatgaatg
     4261 tcagctactg ggctatctgg acaagggaaa acgcaagcgc aaagagaaag caggtagctt
     4321 gcagtgggct tacatggcga tagctagact gggcggtttt atggacagca agcgaaccgg
     4381 aattgccagc tggggcgccc tctggtaagg ttgggaagcc ctgcaaagta aactggatgg
     4441 ctttcttgcc gccaaggatc tgatggcgca ggggatcaag ctctgatcaa gagacaggat
     4501 gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg gccgcttggg
     4561 tggagaggct attcggctat gactgggcac aacagacaat cggctgctct gatgccgccg
     4621 tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac ctgtccggtg
     4681 ccctgaatga actgcaagac gaggcagcgc ggctatcgtg gctggccacg acgggcgttc
     4741 cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg ctattgggcg
     4801 aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa gtatccatca
     4861 tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca ttcgaccacc
     4921 aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt gtcgatcagg
     4981 atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc aggctcaagg
     5041 cgagcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc ttgccgaata
     5101 tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg ggtgtggcgg
     5161 accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt ggcggcgaat
     5221 gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag cgcatcgcct
     5281 tctatcgcct tcttgacgag ttcttctgaa ttattaacgc ttacaatttc ctgatgcggt
     5341 attttctcct tacgcatctg tgcggtattt cacaccgcat caggtggcac ttttcgggga
     5401 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
     5461 atgagacaat aaccctgata aatgcttcaa taatagcacg tgaggagggc caccatggcc
     5521 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcttccaatt agaggttgtg
     5581 gatgcaaaat tgaaaaaaaa atgactattg gtagcgtgtt ctatttttct tatgcgctga
     5641 aaaactacga acaattgtct attccaagcc tgtggctttt tttggtgatt cacaatacat
     5701 aaattggcaa atacctcttc tatagacttt aacctttgat gaaatggtat ggataaacca
     5761 cttttgtggt cagggtatat atatttttat tccaatctgg cctttataaa ttcaaatttc
     5821 caaggggact ctgctaaacc ttctgatata actataactc taataatggc tgtaaaaagt
     5881 gtgaaaaaaa gatgatattc acaaaaaaga taaaggggat ttcatattgg gtatctagat
     5941 ttaaacatcg tatttcaaca atgaaactag aatcaatacg aaatccaggg cacaactgtg
     6001 ttcattccct tgggaattat actgagtaat gggaagaatg tgcaaaaaat acccgtgtct
     6061 tatataattt ccatttgtgt agggcttaaa agtggacaac ttcaggttac gaaaccacga
     6121 aaatgtctaa acgtttgatc aaattcgtaa ctcatggaaa atcacagcaa caaattaagg
     6181 atggatatat ccggtactta caaataaaaa cactaaagaa gtataaattt tgaactgaaa
     6241 ttctatagaa aatctttttt tttgggtaaa attatgcgat tgtggctcat agtaacgtaa
     6301 tcatacagtt caacaaacat cattttgtca aaggatttct aatcctattc tttttctact
     6361 tggcttttga gtatatatat atatatatat acatcctttg ttcatcatca tgttcgattg
     6421 aatttttttc ttggttgtta ttagattgtc tacttgagtt tttcttggtg caaattgtgg
     6481 tgtttttcta acaaattttt caacatttaa agtatttctg gaacttttct acaaggaacg
     6541 acatagatcc attaatgcgc ccctctaaaa caatacccat aatgtcttct taagtatgca
     6601 tctgcttata aatgtttctg gatttacttt agttagaatg tcgacacgac aatgaactat
     6661 tgctgtagat cacgtgcgat cactgaatta tgagtttctt tttatttttc tttttgggct
     6721 tttttttttg caattttttt tttccacctt tgccaattgc gtagtcacat ataatgtata
     6781 caatttttgc aactctgaca ctataattga tccaataata aagtatataa tagatcatca
     6841 agtatacgta atctccccaa aaaaaaaatt gcataaaata aaatctataa acctttttta
     6901 gatttttagt tgttcctttt ttctttctat tcttcaactt ccaagtgttg tcactttctt
     6961 cctgttaact atatttaaaa gtaaataccc tccccagaaa actccggaat ggctgctcct
     7021 ccaaaaaaga agagaaaggt agctagagaa ttaacaaaac gacaaagcga aatatataac
     7081 tatattaaac aagttgttca aatgaaaggt tatccgccta gtgttcgcga aattggtgag
     7141 gcagttggct tagcatccag ttcaactgtt catggtcacc tttcacgtct tgaagaaaaa
     7201 ggctatataa gaagagatcc aacgaaacca cgtgctatag aaattgtaag tgatcaaaca
     7261 aatgataata ttaatatgga agaaacgatt catgtgccag ttattggtaa agtcacagca
     7321 ggtgttccta ttaccgcagt agaaaatatt gaagaatatt ttccattacc tgaacactta
     7381 acatcgacac acaatagcga catattcata ttaaacgtcg taggcgacag tatgattgag
     7441 gctggtatat tagacggaga caaagtaatt gttcgcagtc aaaccatagc agaaaatgga
     7501 gacattattg ttgctatgac tgaggaagat gaagcaactg tcaaacgctt ctataaagaa
     7561 aaaaatcgtt atcgattaca acctgaaaat agtacaatgg agccaattta cctcgacaat
     7621 gttgctgtaa tcgggaaagt aattggtttg taccgcgaaa tgagatctgg tgctggtggt
     7681 gttggttatc cgtatgatgt gcctgactac gcaggtgctg gtggtgttgg tgctagcAGG
     7741 CCTATGACAA CAGCTGACGA ATACAAAGCA GAAGGTAACA AATATTTTGC TGCTAAAGAT
     7801 TTTGAAAAGG CGATTGAAGC ATTCACTAAA GCAATTGAAG CATCACCTGA ACCAAACCAT
     7861 GTTCTTTATT CAAATCGTTC TGGATCTTAT GCCTCTTTAA AAGATTTTAA CAACGCATTA
     7921 AAAGATGCTC AAGAATGTGT CAAGATCAAT CCTAGTTGGG CCAAAGGGTA TAATAGAATT
     7981 GCTGGGGCTG AATTTGGTTT AGGTAATTTT GATCAAGCTA AATCCAATTA TGAAAAATGT
     8041 TTGGAGTTGG ATCCAAATAA TGCCATGGCT AAAGAAGGTT TAAAATCAGT TGAATCTGCT
     8101 TTAGTATCTG GTGGTGGTGA TGATGACAAG GATTTAGGAT TTGGTAAAAT TTTGAATGAT
     8161 CCTAATCTTT ATACTAAATT GAAAAATAAT CCAAAAACAA GTGAATTTAT GAATGATCCT
     8221 CAATTTGTTG CTAAACTTGA ACGTCTTAAA ACTAATCCAC AATTGGGTAA TCCTGATATG
     8281 TTTAGTGATC CAAGATTATT GACGGCTTTT GCTGCTTTAA TGGGTATTGA CATGGATTTA
     8341 CCAAATATGG GATTCACTGC TCCAAACGAA TCACAATCCA ATGCATCAGA ACCAAAACTG
     8401 GAACCAAAAT CAGTACCAGA ATCTAAACCA GAACCAAAAG CAGAACAAAA GGAAGAAGAA
     8461 TCAACCTCAG CCAAAGATGA AGACACTCCA ATGACTGATG CCCAAGACGA CACTAATGAT
     8521 AATGATGCCA AAACCCAAGC TGACAATGCT AAAGCTGAAG GTAATGCCTT ATACAAGAAA
     8581 CGTCAATTTG ATGAAGCAAT TGCCGCCTAT AATAAGGCTT GGGAATTACA TAAGGATATC
     8641 ACTTATTTAA ACAATCGTGC TGCTGCCGAA TATGAAAAAG GTGATTATGA TGCTGCTATT
     8701 GCTACATGTG AAAAGGCCAT TGATGAAGGT AGAGACATGA GAGCTGATTA TAAATTGATT
     8761 GCTAAATCAT TTGCTAGATT AGGTAATATT TATTTGAAAA AAGATGAATT ACCCGAAGCA
     8821 GTGAAAAATT TTGAAAAATC TTTAACTGAA CATCGTACCC CTGATGTTTT AAATAAATTA
     8881 AGATCAACTC AACGTGAAAT TAAAACTAGA GAATTAAATG CTTATATAGA TCCAGAAAAG
     8941 GCTGAAGAAG CAAGATTACA AGGTAAAGAA TATTTCACCA AAGGAGATTG GCCAAATGCC
     9001 GTTAAGGCTT ATACTGAAAT GATTAAAAGA GCACCAGAAG ATGCTAGAGG ATATTCTAAT
     9061 CGTGCTGCTG CATTGGCAAA ATTGTTATCC TTTCCTGATG CTATACAAGA TTGTAATAAA
     9121 GCCATTGAAA AAGATCCAAA TTTCATTAGA GCTTATATTA GAAAAGCTAA TGCTCAATTG
     9181 GCAATGAAAG AATATAGTCA TGTCATGGAT ACTTTAACCG AGGCAAGAAC TAAAGATGTT
     9241 GAATTGGGTG GTAAATCAAT TCATGAAATT GATGAATTAA TGAATAAAGC TACTTATCAA
     9301 AGATTTCAAG CCATTGAAGG TGAAACTCCT GAACAAACTA TGGAAAGAGT TTCTAAAGAT
     9361 CCAGAAATTG TTCAAATTTT ACAAGATCCA GTAATGCAAG GAATTTTAGC TCAAGCTAGA
     9421 GAAAATCCTG CTGCTTTACA AGATCATATG AAAAATCCTG AAGTTTATAA AAAAATTAAT
     9481 ATGTTGATTG CTGCTGGTGT TATTCGTACC AGATAAACGC GTagtactta accgcgccgt
     9541 cgacgagtga aattctggaa atctggaaat ctggttttgt attcttgtta ttcttctttt
     9601 tgttattaca tatataactt gttacttttt taaaaaaatc tttgtttatt ttataaatat
     9661 ataaaactaa atttaagaaa aagagaaaaa tgttttattt gagagattga aattttactt
     9721 gaatttagct tagcttttat aaagtattat tatgtaaaaa aacaaaacaa atatacatta
     9781 aaaagttaag actataaaat agccacccaa ggcatttcta tatcttgttg ttgttgtttt
     9841 catcttctgt atcagaggaa cttattttat tattttcgtc acgggtattt tctcttgttt
     9901 gatgattcat cccattcatt ccatcataaa attctagaag gaccaccttt gattgtaaat
     9961 agtaataatt accaccctta tttaattatt tatttaactt atttatttat ttattataca
    10021 tatatacaaa tctaataaag tgaaaatctc ccccttcaca cgtggccgag gagcaggact
    10081 gacacgtgct aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
    10141 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
    10201 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
    10261 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
    10321 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc
    10381 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
    10441 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
    10501 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
    10561 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
    10621 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
    10681 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
    10741 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
    10801 tatggaaaaa cgccagcaac gcggcctttt tacggttcct gggcttttgc tggccttttg
    10861 ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgcctttg
    10921 agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca gtgagcgagg
    10981 aagcggaag
//
